Changes in mitochondrial DNA levels during early embryogenesis in Torenia fournieri and Arabidopsis thaliana
Summary Changes in the amount of mitochondrial DNA (mtDNA) have never been investigated in plant zygotes or early plant embryos due to the difficulty in isolating these cells, although such changes have been investigated in mammalian embryos. Using the single‐cell quantitative real‐time polymerase c...
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| Veröffentlicht in: | The Plant journal : for cell and molecular biology 2018-09, Vol.95 (5), p.785-795 |
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| container_title | The Plant journal : for cell and molecular biology |
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| creator | Gao, Long Guo, Xue Liu, Xue‐Qiong Zhang, Li Huang, Jilei Tan, Li Lin, Zhen Nagawa, Shingo Wang, Dan‐Yang |
| description | Summary
Changes in the amount of mitochondrial DNA (mtDNA) have never been investigated in plant zygotes or early plant embryos due to the difficulty in isolating these cells, although such changes have been investigated in mammalian embryos. Using the single‐cell quantitative real‐time polymerase chain reaction (PCR) and laser confocal microscopy, we surveyed the changes in mtDNA levels during early embryogenesis in Torenia fournieri and Arabidopsis thaliana. In contrast with the amount of mtDNA in early mammalian embryos, which does not change, we found that mtDNA doubling occurred during zygotic development in T. fournieri and during two‐cell proembryo development in A. thaliana. These findings reveal that mtDNA doubling occurs during early embryogenesis in T. fournieri and A. thaliana, indicating that the dynamics of mtDNA in early plant embryos differs from that in early mammalian embryos.
Significance Statement
Replication of mtDNA occurs during zygotic development in Torenia fournieri and during two‐cell proembryo development in Arabidopsis thaliana. Given that the mtDNA levels do not change during early mammalian embryogenesis, our results reveal that early plant embryos have different mtDNA dynamics from early mammalian embryos. |
| doi_str_mv | 10.1111/tpj.13987 |
| format | Article |
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Changes in the amount of mitochondrial DNA (mtDNA) have never been investigated in plant zygotes or early plant embryos due to the difficulty in isolating these cells, although such changes have been investigated in mammalian embryos. Using the single‐cell quantitative real‐time polymerase chain reaction (PCR) and laser confocal microscopy, we surveyed the changes in mtDNA levels during early embryogenesis in Torenia fournieri and Arabidopsis thaliana. In contrast with the amount of mtDNA in early mammalian embryos, which does not change, we found that mtDNA doubling occurred during zygotic development in T. fournieri and during two‐cell proembryo development in A. thaliana. These findings reveal that mtDNA doubling occurs during early embryogenesis in T. fournieri and A. thaliana, indicating that the dynamics of mtDNA in early plant embryos differs from that in early mammalian embryos.
Significance Statement
Replication of mtDNA occurs during zygotic development in Torenia fournieri and during two‐cell proembryo development in Arabidopsis thaliana. Given that the mtDNA levels do not change during early mammalian embryogenesis, our results reveal that early plant embryos have different mtDNA dynamics from early mammalian embryos.</description><identifier>ISSN: 0960-7412</identifier><identifier>EISSN: 1365-313X</identifier><identifier>DOI: 10.1111/tpj.13987</identifier><identifier>PMID: 29882345</identifier><language>eng</language><publisher>England: Blackwell Publishing Ltd</publisher><subject>Arabidopsis thaliana ; Confocal microscopy ; Deoxyribonucleic acid ; DNA ; early embryogenesis ; Embryogenesis ; Embryonic growth stage ; Embryos ; Genotype & phenotype ; Mammals ; Microscopy ; Mitochondrial DNA ; mtDNA ; Polymerase chain reaction ; replication ; Torenia fournieri ; Zygotes</subject><ispartof>The Plant journal : for cell and molecular biology, 2018-09, Vol.95 (5), p.785-795</ispartof><rights>2018 The Authors The Plant Journal © 2018 John Wiley & Sons Ltd</rights><rights>2018 The Authors The Plant Journal © 2018 John Wiley & Sons Ltd.</rights><rights>Copyright © 2018 John Wiley & Sons Ltd and the Society for Experimental Biology</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4547-ffea37e72739178c2a9d02dc2a5687a9de209fb5f12ad16f7d3f1fd11743661f3</citedby><cites>FETCH-LOGICAL-c4547-ffea37e72739178c2a9d02dc2a5687a9de209fb5f12ad16f7d3f1fd11743661f3</cites><orcidid>0000-0001-8735-0422</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Ftpj.13987$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Ftpj.13987$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1417,1433,27924,27925,45574,45575,46409,46833</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/29882345$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Gao, Long</creatorcontrib><creatorcontrib>Guo, Xue</creatorcontrib><creatorcontrib>Liu, Xue‐Qiong</creatorcontrib><creatorcontrib>Zhang, Li</creatorcontrib><creatorcontrib>Huang, Jilei</creatorcontrib><creatorcontrib>Tan, Li</creatorcontrib><creatorcontrib>Lin, Zhen</creatorcontrib><creatorcontrib>Nagawa, Shingo</creatorcontrib><creatorcontrib>Wang, Dan‐Yang</creatorcontrib><title>Changes in mitochondrial DNA levels during early embryogenesis in Torenia fournieri and Arabidopsis thaliana</title><title>The Plant journal : for cell and molecular biology</title><addtitle>Plant J</addtitle><description>Summary
Changes in the amount of mitochondrial DNA (mtDNA) have never been investigated in plant zygotes or early plant embryos due to the difficulty in isolating these cells, although such changes have been investigated in mammalian embryos. Using the single‐cell quantitative real‐time polymerase chain reaction (PCR) and laser confocal microscopy, we surveyed the changes in mtDNA levels during early embryogenesis in Torenia fournieri and Arabidopsis thaliana. In contrast with the amount of mtDNA in early mammalian embryos, which does not change, we found that mtDNA doubling occurred during zygotic development in T. fournieri and during two‐cell proembryo development in A. thaliana. These findings reveal that mtDNA doubling occurs during early embryogenesis in T. fournieri and A. thaliana, indicating that the dynamics of mtDNA in early plant embryos differs from that in early mammalian embryos.
Significance Statement
Replication of mtDNA occurs during zygotic development in Torenia fournieri and during two‐cell proembryo development in Arabidopsis thaliana. Given that the mtDNA levels do not change during early mammalian embryogenesis, our results reveal that early plant embryos have different mtDNA dynamics from early mammalian embryos.</description><subject>Arabidopsis thaliana</subject><subject>Confocal microscopy</subject><subject>Deoxyribonucleic acid</subject><subject>DNA</subject><subject>early embryogenesis</subject><subject>Embryogenesis</subject><subject>Embryonic growth stage</subject><subject>Embryos</subject><subject>Genotype & phenotype</subject><subject>Mammals</subject><subject>Microscopy</subject><subject>Mitochondrial DNA</subject><subject>mtDNA</subject><subject>Polymerase chain reaction</subject><subject>replication</subject><subject>Torenia fournieri</subject><subject>Zygotes</subject><issn>0960-7412</issn><issn>1365-313X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2018</creationdate><recordtype>article</recordtype><recordid>eNp10U1PGzEQBmALgUqgHPgDlSUucFjwx-7ae4xC-RKiPaQSt5WzHieOvHZqZ4vy73EI5YCELyNLj195ZhA6peSS5nO1Xi0vKW-k2EMjyuuq4JQ_76MRaWpSiJKyQ3SU0pIQKnhdfkOHrJGS8bIaITdZKD-HhK3HvV2HbhG8jlY5fP00xg7-gUtYD9H6OQYV3QZDP4ubMAcPyb49m4YI3ipswhC9hWix8hqPo5pZHVZbtF4oZ5VX39GBUS7ByXs9Rn9ufk4nd8Xjr9v7yfix6MqqFIUxoLgAwQRvqJAdU40mTOda1VLkCzDSmFllKFOa1kZobqjRlIqS1zU1_Bid73JXMfwdIK3b3qYOnFMewpBaRiomKatklenZJ7rctpF_l5WUomR5fFld7FQXQ0oRTLuKtldx01LSblfQ5hW0byvI9sd74jDrQX_I_zPP4GoHXqyDzddJ7fT3wy7yFWMBkPE</recordid><startdate>201809</startdate><enddate>201809</enddate><creator>Gao, Long</creator><creator>Guo, Xue</creator><creator>Liu, Xue‐Qiong</creator><creator>Zhang, Li</creator><creator>Huang, Jilei</creator><creator>Tan, Li</creator><creator>Lin, Zhen</creator><creator>Nagawa, Shingo</creator><creator>Wang, Dan‐Yang</creator><general>Blackwell Publishing Ltd</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>7QP</scope><scope>7QR</scope><scope>7TM</scope><scope>8FD</scope><scope>FR3</scope><scope>M7N</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0001-8735-0422</orcidid></search><sort><creationdate>201809</creationdate><title>Changes in mitochondrial DNA levels during early embryogenesis in Torenia fournieri and Arabidopsis thaliana</title><author>Gao, Long ; Guo, Xue ; Liu, Xue‐Qiong ; Zhang, Li ; Huang, Jilei ; Tan, Li ; Lin, Zhen ; Nagawa, Shingo ; Wang, Dan‐Yang</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4547-ffea37e72739178c2a9d02dc2a5687a9de209fb5f12ad16f7d3f1fd11743661f3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2018</creationdate><topic>Arabidopsis thaliana</topic><topic>Confocal microscopy</topic><topic>Deoxyribonucleic acid</topic><topic>DNA</topic><topic>early embryogenesis</topic><topic>Embryogenesis</topic><topic>Embryonic growth stage</topic><topic>Embryos</topic><topic>Genotype & phenotype</topic><topic>Mammals</topic><topic>Microscopy</topic><topic>Mitochondrial DNA</topic><topic>mtDNA</topic><topic>Polymerase chain reaction</topic><topic>replication</topic><topic>Torenia fournieri</topic><topic>Zygotes</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Gao, Long</creatorcontrib><creatorcontrib>Guo, Xue</creatorcontrib><creatorcontrib>Liu, Xue‐Qiong</creatorcontrib><creatorcontrib>Zhang, Li</creatorcontrib><creatorcontrib>Huang, Jilei</creatorcontrib><creatorcontrib>Tan, Li</creatorcontrib><creatorcontrib>Lin, Zhen</creatorcontrib><creatorcontrib>Nagawa, Shingo</creatorcontrib><creatorcontrib>Wang, Dan‐Yang</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>Biotechnology Research Abstracts</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Chemoreception Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>The Plant journal : for cell and molecular biology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Gao, Long</au><au>Guo, Xue</au><au>Liu, Xue‐Qiong</au><au>Zhang, Li</au><au>Huang, Jilei</au><au>Tan, Li</au><au>Lin, Zhen</au><au>Nagawa, Shingo</au><au>Wang, Dan‐Yang</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Changes in mitochondrial DNA levels during early embryogenesis in Torenia fournieri and Arabidopsis thaliana</atitle><jtitle>The Plant journal : for cell and molecular biology</jtitle><addtitle>Plant J</addtitle><date>2018-09</date><risdate>2018</risdate><volume>95</volume><issue>5</issue><spage>785</spage><epage>795</epage><pages>785-795</pages><issn>0960-7412</issn><eissn>1365-313X</eissn><abstract>Summary
Changes in the amount of mitochondrial DNA (mtDNA) have never been investigated in plant zygotes or early plant embryos due to the difficulty in isolating these cells, although such changes have been investigated in mammalian embryos. Using the single‐cell quantitative real‐time polymerase chain reaction (PCR) and laser confocal microscopy, we surveyed the changes in mtDNA levels during early embryogenesis in Torenia fournieri and Arabidopsis thaliana. In contrast with the amount of mtDNA in early mammalian embryos, which does not change, we found that mtDNA doubling occurred during zygotic development in T. fournieri and during two‐cell proembryo development in A. thaliana. These findings reveal that mtDNA doubling occurs during early embryogenesis in T. fournieri and A. thaliana, indicating that the dynamics of mtDNA in early plant embryos differs from that in early mammalian embryos.
Significance Statement
Replication of mtDNA occurs during zygotic development in Torenia fournieri and during two‐cell proembryo development in Arabidopsis thaliana. Given that the mtDNA levels do not change during early mammalian embryogenesis, our results reveal that early plant embryos have different mtDNA dynamics from early mammalian embryos.</abstract><cop>England</cop><pub>Blackwell Publishing Ltd</pub><pmid>29882345</pmid><doi>10.1111/tpj.13987</doi><tpages>11</tpages><orcidid>https://orcid.org/0000-0001-8735-0422</orcidid><oa>free_for_read</oa></addata></record> |
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| subjects | Arabidopsis thaliana Confocal microscopy Deoxyribonucleic acid DNA early embryogenesis Embryogenesis Embryonic growth stage Embryos Genotype & phenotype Mammals Microscopy Mitochondrial DNA mtDNA Polymerase chain reaction replication Torenia fournieri Zygotes |
| title | Changes in mitochondrial DNA levels during early embryogenesis in Torenia fournieri and Arabidopsis thaliana |
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