Optimization of activation, collection, dilution, and storage methods for zebrafish sperm
In the present study, we optimized activation, collection, dilution, and storage methods for zebrafish ( Danio rerio) sperm. Our findings revealed that zebrafish sperm was motile in Hank's balanced salt solution (HBSS), glucose, and sucrose at an osmolality range of 25–270 mOsm/kg with the high...
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| Veröffentlicht in: | Aquaculture 2009-05, Vol.290 (1), p.165-171 |
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| creator | Jing, Rongyan Huang, Changjiang Bai, Chenglian Tanguay, Robert Dong, Qiaoxiang |
| description | In the present study, we optimized activation, collection, dilution, and storage methods for zebrafish (
Danio rerio) sperm. Our findings revealed that zebrafish sperm was motile in Hank's balanced salt solution (HBSS), glucose, and sucrose at an osmolality range of 25–270 mOsm/kg with the highest motility observed at 150–210 mOsm/kg. We compared three activation solutions: 0.3% NaCl, de-ionized water, and HBSS at 170 mOsm/kg. Our results indicate that the longevity of sperm motility depends on the final osmolality of the sperm suspension medium (extender) and activation solution combined. Evaluation of sperm collection methods suggested that higher percent motility is obtained when collected through dissecting without crushing (89
±
3%, mean
±
SD) or abdominal massage (90
±
4%) than dissecting with crushing (65
±
13%). The total number of motile sperm was higher for dissecting without crushing (147.0
±
102.3
×
10
5/male) than abdominal massage (7.1
±
11.9
×
10
5/male). Sperm suspended in HBSS had higher motility than those suspended in buffered sperm motility-inhibiting solution (BSMIS). Sperm retained motility longer when samples were stored at 4 °C than those stored at room temperature (25 °C). Motility which could be activated after storage decreased with increased dilution ratio and it is the resultant sperm density rather than the dilution factor that determines sperm motility. Our findings provide useful information on handling procedures of zebrafish sperm. |
| doi_str_mv | 10.1016/j.aquaculture.2009.02.027 |
| format | Article |
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Danio rerio) sperm. Our findings revealed that zebrafish sperm was motile in Hank's balanced salt solution (HBSS), glucose, and sucrose at an osmolality range of 25–270 mOsm/kg with the highest motility observed at 150–210 mOsm/kg. We compared three activation solutions: 0.3% NaCl, de-ionized water, and HBSS at 170 mOsm/kg. Our results indicate that the longevity of sperm motility depends on the final osmolality of the sperm suspension medium (extender) and activation solution combined. Evaluation of sperm collection methods suggested that higher percent motility is obtained when collected through dissecting without crushing (89
±
3%, mean
±
SD) or abdominal massage (90
±
4%) than dissecting with crushing (65
±
13%). The total number of motile sperm was higher for dissecting without crushing (147.0
±
102.3
×
10
5/male) than abdominal massage (7.1
±
11.9
×
10
5/male). Sperm suspended in HBSS had higher motility than those suspended in buffered sperm motility-inhibiting solution (BSMIS). Sperm retained motility longer when samples were stored at 4 °C than those stored at room temperature (25 °C). Motility which could be activated after storage decreased with increased dilution ratio and it is the resultant sperm density rather than the dilution factor that determines sperm motility. Our findings provide useful information on handling procedures of zebrafish sperm.</description><identifier>ISSN: 0044-8486</identifier><identifier>EISSN: 1873-5622</identifier><identifier>DOI: 10.1016/j.aquaculture.2009.02.027</identifier><identifier>CODEN: AQCLAL</identifier><language>eng</language><publisher>Amsterdam: Elsevier B.V</publisher><subject>Animal aquaculture ; Animal productions ; Animal reproduction ; Aquaculture ; Biological and medical sciences ; Danio rerio ; fish culture ; Freshwater ; freshwater aquaculture ; freshwater fish ; Fundamental and applied biological sciences. Psychology ; General aspects ; Glucose ; Motility ; Optimization ; Osmolality ; Salt ; semen extenders ; Sperm collection ; sperm motility ; spermatozoa ; storage temperature ; Studies ; Temperature ; Zebrafish</subject><ispartof>Aquaculture, 2009-05, Vol.290 (1), p.165-171</ispartof><rights>2009 Elsevier B.V.</rights><rights>2009 INIST-CNRS</rights><rights>Copyright Elsevier Sequoia S.A. May 4, 2009</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c499t-407b4a2c2cbaef4a0f539941cb25bd0c8be20626310fcaa3950f818ae138cecc3</citedby><cites>FETCH-LOGICAL-c499t-407b4a2c2cbaef4a0f539941cb25bd0c8be20626310fcaa3950f818ae138cecc3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.aquaculture.2009.02.027$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3549,27923,27924,45994</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=21378000$$DView record in Pascal Francis$$Hfree_for_read</backlink></links><search><creatorcontrib>Jing, Rongyan</creatorcontrib><creatorcontrib>Huang, Changjiang</creatorcontrib><creatorcontrib>Bai, Chenglian</creatorcontrib><creatorcontrib>Tanguay, Robert</creatorcontrib><creatorcontrib>Dong, Qiaoxiang</creatorcontrib><title>Optimization of activation, collection, dilution, and storage methods for zebrafish sperm</title><title>Aquaculture</title><description>In the present study, we optimized activation, collection, dilution, and storage methods for zebrafish (
Danio rerio) sperm. Our findings revealed that zebrafish sperm was motile in Hank's balanced salt solution (HBSS), glucose, and sucrose at an osmolality range of 25–270 mOsm/kg with the highest motility observed at 150–210 mOsm/kg. We compared three activation solutions: 0.3% NaCl, de-ionized water, and HBSS at 170 mOsm/kg. Our results indicate that the longevity of sperm motility depends on the final osmolality of the sperm suspension medium (extender) and activation solution combined. Evaluation of sperm collection methods suggested that higher percent motility is obtained when collected through dissecting without crushing (89
±
3%, mean
±
SD) or abdominal massage (90
±
4%) than dissecting with crushing (65
±
13%). The total number of motile sperm was higher for dissecting without crushing (147.0
±
102.3
×
10
5/male) than abdominal massage (7.1
±
11.9
×
10
5/male). Sperm suspended in HBSS had higher motility than those suspended in buffered sperm motility-inhibiting solution (BSMIS). Sperm retained motility longer when samples were stored at 4 °C than those stored at room temperature (25 °C). Motility which could be activated after storage decreased with increased dilution ratio and it is the resultant sperm density rather than the dilution factor that determines sperm motility. Our findings provide useful information on handling procedures of zebrafish sperm.</description><subject>Animal aquaculture</subject><subject>Animal productions</subject><subject>Animal reproduction</subject><subject>Aquaculture</subject><subject>Biological and medical sciences</subject><subject>Danio rerio</subject><subject>fish culture</subject><subject>Freshwater</subject><subject>freshwater aquaculture</subject><subject>freshwater fish</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>General aspects</subject><subject>Glucose</subject><subject>Motility</subject><subject>Optimization</subject><subject>Osmolality</subject><subject>Salt</subject><subject>semen extenders</subject><subject>Sperm collection</subject><subject>sperm motility</subject><subject>spermatozoa</subject><subject>storage temperature</subject><subject>Studies</subject><subject>Temperature</subject><subject>Zebrafish</subject><issn>0044-8486</issn><issn>1873-5622</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2009</creationdate><recordtype>article</recordtype><recordid>eNqNkVFr1UAQhYNY8Nr6G4yCPjXX2c0m2TzKRatQ6EPtg0_LZDLb7iXJ3u4mBfvr3WuKiE-FgZmBjzOHM1n2TsBWgKg_7bd4vyAtw7wE3kqAdgsyVfMi2wjdlEVVS_ky2wAoVWil61fZ6xj3AFDXldhkP68OsxvdI87OT7m3OdLsHv5s5zn5YWBa594Nyzrh1Odx9gFvOR95vvN9zK0P-SN3Aa2Ld3k8cBjPshOLQ-Q3T_00u_n65cfuW3F5dfF99_myINW2c6Gg6RRKktQhW4Vgq7JtlaBOVl0PpDuWUMu6FGAJsWwrsFpoZFFqYqLyNPu46h6Cv184zmZ0kXgYcGK_RCOhErqsmwS-_w_c-yVMyVtiVJM4rRLUrhAFH2Ngaw7BjRh-GQHmmLjZm38SN8fEDchUxwMfng5gJBxswIlc_CsgRdnoFHzi3q6cRW_wNiTm5lqCKJO8qJPXROxWglNwD46DieR4Iu5dSA8xvXfP8PMbfp2ncA</recordid><startdate>20090504</startdate><enddate>20090504</enddate><creator>Jing, Rongyan</creator><creator>Huang, Changjiang</creator><creator>Bai, Chenglian</creator><creator>Tanguay, Robert</creator><creator>Dong, Qiaoxiang</creator><general>Elsevier B.V</general><general>Amsterdam: Elsevier Science</general><general>Elsevier</general><general>Elsevier Sequoia S.A</general><scope>FBQ</scope><scope>IQODW</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7QR</scope><scope>7ST</scope><scope>7TN</scope><scope>7U7</scope><scope>7U9</scope><scope>8FD</scope><scope>C1K</scope><scope>F1W</scope><scope>FR3</scope><scope>H94</scope><scope>H95</scope><scope>H98</scope><scope>H99</scope><scope>L.F</scope><scope>L.G</scope><scope>M7N</scope><scope>P64</scope><scope>SOI</scope></search><sort><creationdate>20090504</creationdate><title>Optimization of activation, collection, dilution, and storage methods for zebrafish sperm</title><author>Jing, Rongyan ; Huang, Changjiang ; Bai, Chenglian ; Tanguay, Robert ; Dong, Qiaoxiang</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c499t-407b4a2c2cbaef4a0f539941cb25bd0c8be20626310fcaa3950f818ae138cecc3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2009</creationdate><topic>Animal aquaculture</topic><topic>Animal productions</topic><topic>Animal reproduction</topic><topic>Aquaculture</topic><topic>Biological and medical sciences</topic><topic>Danio rerio</topic><topic>fish culture</topic><topic>Freshwater</topic><topic>freshwater aquaculture</topic><topic>freshwater fish</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>General aspects</topic><topic>Glucose</topic><topic>Motility</topic><topic>Optimization</topic><topic>Osmolality</topic><topic>Salt</topic><topic>semen extenders</topic><topic>Sperm collection</topic><topic>sperm motility</topic><topic>spermatozoa</topic><topic>storage temperature</topic><topic>Studies</topic><topic>Temperature</topic><topic>Zebrafish</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Jing, Rongyan</creatorcontrib><creatorcontrib>Huang, Changjiang</creatorcontrib><creatorcontrib>Bai, Chenglian</creatorcontrib><creatorcontrib>Tanguay, Robert</creatorcontrib><creatorcontrib>Dong, Qiaoxiang</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Chemoreception Abstracts</collection><collection>Environment Abstracts</collection><collection>Oceanic Abstracts</collection><collection>Toxicology Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ASFA: Aquatic Sciences and Fisheries Abstracts</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) 1: Biological Sciences & Living Resources</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Aquaculture Abstracts</collection><collection>ASFA: Marine Biotechnology Abstracts</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Marine Biotechnology Abstracts</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Professional</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Environment Abstracts</collection><jtitle>Aquaculture</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Jing, Rongyan</au><au>Huang, Changjiang</au><au>Bai, Chenglian</au><au>Tanguay, Robert</au><au>Dong, Qiaoxiang</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Optimization of activation, collection, dilution, and storage methods for zebrafish sperm</atitle><jtitle>Aquaculture</jtitle><date>2009-05-04</date><risdate>2009</risdate><volume>290</volume><issue>1</issue><spage>165</spage><epage>171</epage><pages>165-171</pages><issn>0044-8486</issn><eissn>1873-5622</eissn><coden>AQCLAL</coden><abstract>In the present study, we optimized activation, collection, dilution, and storage methods for zebrafish (
Danio rerio) sperm. Our findings revealed that zebrafish sperm was motile in Hank's balanced salt solution (HBSS), glucose, and sucrose at an osmolality range of 25–270 mOsm/kg with the highest motility observed at 150–210 mOsm/kg. We compared three activation solutions: 0.3% NaCl, de-ionized water, and HBSS at 170 mOsm/kg. Our results indicate that the longevity of sperm motility depends on the final osmolality of the sperm suspension medium (extender) and activation solution combined. Evaluation of sperm collection methods suggested that higher percent motility is obtained when collected through dissecting without crushing (89
±
3%, mean
±
SD) or abdominal massage (90
±
4%) than dissecting with crushing (65
±
13%). The total number of motile sperm was higher for dissecting without crushing (147.0
±
102.3
×
10
5/male) than abdominal massage (7.1
±
11.9
×
10
5/male). Sperm suspended in HBSS had higher motility than those suspended in buffered sperm motility-inhibiting solution (BSMIS). Sperm retained motility longer when samples were stored at 4 °C than those stored at room temperature (25 °C). Motility which could be activated after storage decreased with increased dilution ratio and it is the resultant sperm density rather than the dilution factor that determines sperm motility. Our findings provide useful information on handling procedures of zebrafish sperm.</abstract><cop>Amsterdam</cop><pub>Elsevier B.V</pub><doi>10.1016/j.aquaculture.2009.02.027</doi><tpages>7</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0044-8486 |
| ispartof | Aquaculture, 2009-05, Vol.290 (1), p.165-171 |
| issn | 0044-8486 1873-5622 |
| language | eng |
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| source | ScienceDirect Journals (5 years ago - present) |
| subjects | Animal aquaculture Animal productions Animal reproduction Aquaculture Biological and medical sciences Danio rerio fish culture Freshwater freshwater aquaculture freshwater fish Fundamental and applied biological sciences. Psychology General aspects Glucose Motility Optimization Osmolality Salt semen extenders Sperm collection sperm motility spermatozoa storage temperature Studies Temperature Zebrafish |
| title | Optimization of activation, collection, dilution, and storage methods for zebrafish sperm |
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