Rapid purification of billions of circulating CD19+ B cells directly from leukophoresis samples
•Purification of large numbers of CD19+ B cells directly from leukophoresis samples.•Excellent source of V genes to create in vitro antibody repertoires or study humoral immune responses.•Unbiased enrichment of natural B cell subpopulations. The study of the biology and function of B cells, or the d...
Gespeichert in:
| Veröffentlicht in: | New biotechnology 2018-11, Vol.46, p.14-21 |
|---|---|
| Hauptverfasser: | , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 21 |
|---|---|
| container_issue | |
| container_start_page | 14 |
| container_title | New biotechnology |
| container_volume | 46 |
| creator | Ferrara, Fortunato Kolnik, Martin D’Angelo, Sara Erasmus, Frank M. Vorholt, Daniela Bradbury, Andrew R.M. |
| description | •Purification of large numbers of CD19+ B cells directly from leukophoresis samples.•Excellent source of V genes to create in vitro antibody repertoires or study humoral immune responses.•Unbiased enrichment of natural B cell subpopulations.
The study of the biology and function of B cells, or the dissection and in vitro creation of enormous recombinant antibody repertoires, requires the isolation of large numbers of pure CD19+ B cells. The StraightFrom® Leukopak CD19 MicroBead Kit was recently introduced as a fast and robust kit to isolate human CD19+ B cells. This uses paramagnetic microbeads conjugated to high-affinity anti-CD19 monoclonal antibodies to bind B cells in leukapheresis (Leukopak) samples. The overall purity of the isolated cells, together with the characterization of the different CD19+ subclasses, was assessed by flow cytometry using a recombinant (REAffinity) antibody panel, revealing that the method allowed the recovery of over 93% of CD19+ B cells without any pre-purification step. This enables the relatively straightforward purification of all the circulating CD19+ B cells in a single donor. |
| doi_str_mv | 10.1016/j.nbt.2018.05.006 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_2051072243</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>S1871678418302541</els_id><sourcerecordid>2051072243</sourcerecordid><originalsourceid>FETCH-LOGICAL-c418t-39aff83fc0b3a3eca82b501b95e30d7454cdf92c83682303f4a6836049872b5f3</originalsourceid><addsrcrecordid>eNp9kMtKxDAUhoMo3h_AjQTcCNKaW9sUVzpeQRBE1yFNE82YNjVpBd_ejDO6cOEqP-Q7P-d8ABxglGOEy9N53jdjThDmOSpyhMo1sI15VWaMsmr9O-OsrDjbAjsxzhOA6xJvgi1S8wpVvNgG4lEOtoXDFKyxSo7W99Ab2FjnUoyLrGxQk0tf_QucXeL6BF5ApZ2LsLVBq9F9QhN8B52e3vzw6oOONsIou8HpuAc2jHRR76_eXfB8ffU0u83uH27uZuf3mWKYjxmtpTGcGoUaKqlWkpOmQLipC01RW7GCqdbURHFackIRNUyWKSOWDkmkobvgeNk7BP8-6TiKzsbFlrLXfoqCoAKjihBGE3r0B537KfRpO0EwpkXNWF0mCi8pFXyMQRsxBNvJ8CkwEgv7Yi6SfbGwL1Ahktw0c7hqnppOt78TP7oTcLYEdFLxYXUQUVndK700KVpv_6n_Am_nlCw</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2113594496</pqid></control><display><type>article</type><title>Rapid purification of billions of circulating CD19+ B cells directly from leukophoresis samples</title><source>Elsevier ScienceDirect Journals</source><creator>Ferrara, Fortunato ; Kolnik, Martin ; D’Angelo, Sara ; Erasmus, Frank M. ; Vorholt, Daniela ; Bradbury, Andrew R.M.</creator><creatorcontrib>Ferrara, Fortunato ; Kolnik, Martin ; D’Angelo, Sara ; Erasmus, Frank M. ; Vorholt, Daniela ; Bradbury, Andrew R.M.</creatorcontrib><description>•Purification of large numbers of CD19+ B cells directly from leukophoresis samples.•Excellent source of V genes to create in vitro antibody repertoires or study humoral immune responses.•Unbiased enrichment of natural B cell subpopulations.
The study of the biology and function of B cells, or the dissection and in vitro creation of enormous recombinant antibody repertoires, requires the isolation of large numbers of pure CD19+ B cells. The StraightFrom® Leukopak CD19 MicroBead Kit was recently introduced as a fast and robust kit to isolate human CD19+ B cells. This uses paramagnetic microbeads conjugated to high-affinity anti-CD19 monoclonal antibodies to bind B cells in leukapheresis (Leukopak) samples. The overall purity of the isolated cells, together with the characterization of the different CD19+ subclasses, was assessed by flow cytometry using a recombinant (REAffinity) antibody panel, revealing that the method allowed the recovery of over 93% of CD19+ B cells without any pre-purification step. This enables the relatively straightforward purification of all the circulating CD19+ B cells in a single donor.</description><identifier>ISSN: 1871-6784</identifier><identifier>EISSN: 1876-4347</identifier><identifier>DOI: 10.1016/j.nbt.2018.05.006</identifier><identifier>PMID: 29870785</identifier><language>eng</language><publisher>Netherlands: Elsevier B.V</publisher><subject>B cells ; Biotechnology ; CD19 antigen ; Cells ; Flow cytometry ; Immunoglobulins ; Leukapheresis ; Lymphocytes B ; Magnetic bead purification ; Magnetism ; Microspheres ; Monoclonal antibodies ; Nanoparticles ; Purification ; Recombinant antibodies</subject><ispartof>New biotechnology, 2018-11, Vol.46, p.14-21</ispartof><rights>2018</rights><rights>Copyright © 2018. Published by Elsevier B.V.</rights><rights>Copyright Elsevier Science Ltd. Nov 25, 2018</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c418t-39aff83fc0b3a3eca82b501b95e30d7454cdf92c83682303f4a6836049872b5f3</citedby><cites>FETCH-LOGICAL-c418t-39aff83fc0b3a3eca82b501b95e30d7454cdf92c83682303f4a6836049872b5f3</cites><orcidid>0000-0002-8046-5048</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.nbt.2018.05.006$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3536,27903,27904,45974</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/29870785$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Ferrara, Fortunato</creatorcontrib><creatorcontrib>Kolnik, Martin</creatorcontrib><creatorcontrib>D’Angelo, Sara</creatorcontrib><creatorcontrib>Erasmus, Frank M.</creatorcontrib><creatorcontrib>Vorholt, Daniela</creatorcontrib><creatorcontrib>Bradbury, Andrew R.M.</creatorcontrib><title>Rapid purification of billions of circulating CD19+ B cells directly from leukophoresis samples</title><title>New biotechnology</title><addtitle>N Biotechnol</addtitle><description>•Purification of large numbers of CD19+ B cells directly from leukophoresis samples.•Excellent source of V genes to create in vitro antibody repertoires or study humoral immune responses.•Unbiased enrichment of natural B cell subpopulations.
The study of the biology and function of B cells, or the dissection and in vitro creation of enormous recombinant antibody repertoires, requires the isolation of large numbers of pure CD19+ B cells. The StraightFrom® Leukopak CD19 MicroBead Kit was recently introduced as a fast and robust kit to isolate human CD19+ B cells. This uses paramagnetic microbeads conjugated to high-affinity anti-CD19 monoclonal antibodies to bind B cells in leukapheresis (Leukopak) samples. The overall purity of the isolated cells, together with the characterization of the different CD19+ subclasses, was assessed by flow cytometry using a recombinant (REAffinity) antibody panel, revealing that the method allowed the recovery of over 93% of CD19+ B cells without any pre-purification step. This enables the relatively straightforward purification of all the circulating CD19+ B cells in a single donor.</description><subject>B cells</subject><subject>Biotechnology</subject><subject>CD19 antigen</subject><subject>Cells</subject><subject>Flow cytometry</subject><subject>Immunoglobulins</subject><subject>Leukapheresis</subject><subject>Lymphocytes B</subject><subject>Magnetic bead purification</subject><subject>Magnetism</subject><subject>Microspheres</subject><subject>Monoclonal antibodies</subject><subject>Nanoparticles</subject><subject>Purification</subject><subject>Recombinant antibodies</subject><issn>1871-6784</issn><issn>1876-4347</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2018</creationdate><recordtype>article</recordtype><recordid>eNp9kMtKxDAUhoMo3h_AjQTcCNKaW9sUVzpeQRBE1yFNE82YNjVpBd_ejDO6cOEqP-Q7P-d8ABxglGOEy9N53jdjThDmOSpyhMo1sI15VWaMsmr9O-OsrDjbAjsxzhOA6xJvgi1S8wpVvNgG4lEOtoXDFKyxSo7W99Ab2FjnUoyLrGxQk0tf_QucXeL6BF5ApZ2LsLVBq9F9QhN8B52e3vzw6oOONsIou8HpuAc2jHRR76_eXfB8ffU0u83uH27uZuf3mWKYjxmtpTGcGoUaKqlWkpOmQLipC01RW7GCqdbURHFackIRNUyWKSOWDkmkobvgeNk7BP8-6TiKzsbFlrLXfoqCoAKjihBGE3r0B537KfRpO0EwpkXNWF0mCi8pFXyMQRsxBNvJ8CkwEgv7Yi6SfbGwL1Ahktw0c7hqnppOt78TP7oTcLYEdFLxYXUQUVndK700KVpv_6n_Am_nlCw</recordid><startdate>20181125</startdate><enddate>20181125</enddate><creator>Ferrara, Fortunato</creator><creator>Kolnik, Martin</creator><creator>D’Angelo, Sara</creator><creator>Erasmus, Frank M.</creator><creator>Vorholt, Daniela</creator><creator>Bradbury, Andrew R.M.</creator><general>Elsevier B.V</general><general>Elsevier Science Ltd</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0002-8046-5048</orcidid></search><sort><creationdate>20181125</creationdate><title>Rapid purification of billions of circulating CD19+ B cells directly from leukophoresis samples</title><author>Ferrara, Fortunato ; Kolnik, Martin ; D’Angelo, Sara ; Erasmus, Frank M. ; Vorholt, Daniela ; Bradbury, Andrew R.M.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c418t-39aff83fc0b3a3eca82b501b95e30d7454cdf92c83682303f4a6836049872b5f3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2018</creationdate><topic>B cells</topic><topic>Biotechnology</topic><topic>CD19 antigen</topic><topic>Cells</topic><topic>Flow cytometry</topic><topic>Immunoglobulins</topic><topic>Leukapheresis</topic><topic>Lymphocytes B</topic><topic>Magnetic bead purification</topic><topic>Magnetism</topic><topic>Microspheres</topic><topic>Monoclonal antibodies</topic><topic>Nanoparticles</topic><topic>Purification</topic><topic>Recombinant antibodies</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Ferrara, Fortunato</creatorcontrib><creatorcontrib>Kolnik, Martin</creatorcontrib><creatorcontrib>D’Angelo, Sara</creatorcontrib><creatorcontrib>Erasmus, Frank M.</creatorcontrib><creatorcontrib>Vorholt, Daniela</creatorcontrib><creatorcontrib>Bradbury, Andrew R.M.</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>Biotechnology Research Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>New biotechnology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ferrara, Fortunato</au><au>Kolnik, Martin</au><au>D’Angelo, Sara</au><au>Erasmus, Frank M.</au><au>Vorholt, Daniela</au><au>Bradbury, Andrew R.M.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Rapid purification of billions of circulating CD19+ B cells directly from leukophoresis samples</atitle><jtitle>New biotechnology</jtitle><addtitle>N Biotechnol</addtitle><date>2018-11-25</date><risdate>2018</risdate><volume>46</volume><spage>14</spage><epage>21</epage><pages>14-21</pages><issn>1871-6784</issn><eissn>1876-4347</eissn><abstract>•Purification of large numbers of CD19+ B cells directly from leukophoresis samples.•Excellent source of V genes to create in vitro antibody repertoires or study humoral immune responses.•Unbiased enrichment of natural B cell subpopulations.
The study of the biology and function of B cells, or the dissection and in vitro creation of enormous recombinant antibody repertoires, requires the isolation of large numbers of pure CD19+ B cells. The StraightFrom® Leukopak CD19 MicroBead Kit was recently introduced as a fast and robust kit to isolate human CD19+ B cells. This uses paramagnetic microbeads conjugated to high-affinity anti-CD19 monoclonal antibodies to bind B cells in leukapheresis (Leukopak) samples. The overall purity of the isolated cells, together with the characterization of the different CD19+ subclasses, was assessed by flow cytometry using a recombinant (REAffinity) antibody panel, revealing that the method allowed the recovery of over 93% of CD19+ B cells without any pre-purification step. This enables the relatively straightforward purification of all the circulating CD19+ B cells in a single donor.</abstract><cop>Netherlands</cop><pub>Elsevier B.V</pub><pmid>29870785</pmid><doi>10.1016/j.nbt.2018.05.006</doi><tpages>8</tpages><orcidid>https://orcid.org/0000-0002-8046-5048</orcidid></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1871-6784 |
| ispartof | New biotechnology, 2018-11, Vol.46, p.14-21 |
| issn | 1871-6784 1876-4347 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_2051072243 |
| source | Elsevier ScienceDirect Journals |
| subjects | B cells Biotechnology CD19 antigen Cells Flow cytometry Immunoglobulins Leukapheresis Lymphocytes B Magnetic bead purification Magnetism Microspheres Monoclonal antibodies Nanoparticles Purification Recombinant antibodies |
| title | Rapid purification of billions of circulating CD19+ B cells directly from leukophoresis samples |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-23T10%3A41%3A47IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Rapid%20purification%20of%20billions%20of%20circulating%20CD19+%20B%20cells%20directly%20from%20leukophoresis%20samples&rft.jtitle=New%20biotechnology&rft.au=Ferrara,%20Fortunato&rft.date=2018-11-25&rft.volume=46&rft.spage=14&rft.epage=21&rft.pages=14-21&rft.issn=1871-6784&rft.eissn=1876-4347&rft_id=info:doi/10.1016/j.nbt.2018.05.006&rft_dat=%3Cproquest_cross%3E2051072243%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2113594496&rft_id=info:pmid/29870785&rft_els_id=S1871678418302541&rfr_iscdi=true |