An Investigation of the Effects of Testosterone and Behavioral Expressions of Pain on Sex/Gender Differences in Pain Perception

Research indicates that women are more susceptible to pain than men, but the reason for this difference is unclear. While estrogen and progesterone have been implicated, testosterone has not received adequate consideration in the literature. Additionally, incorporating behavioral expressions, or exaggerations, of pain as an important aspect of pain perception is receiving increasing attention. The current study examined the role of testosterone in female pain expression and perception via the cold pressor test. Following all participant exclusions, 46 healthy participants (32 women) provided saliva samples for testosterone analysis using enzyme-linked immunosorbent assay before and after rating their pain during the cold pressor test. Participants used a visual analog scale to indicate how the 2℃ water was perceived, ranging from “worst pain imaginable” to “no pain.” The researcher also noted whether a participant displayed overt behavioral expressions of pain such as jumping and cursing. The results revealed that men reported lower visual analog scale scores than women, indicating less perceived pain. A subgroup of women who displayed overt behavioral responses to pain seemed to be driving this sex/gender difference. It was expected that this subgroup of females would have corresponding changes in testosterone that would further explain the observed sex/gender differences, but this was not supported. Collectively, these data add to the previous literature investigating sex/gender differences in pain perception and highlight the importance of studying overt behavioral expressions of pain. Testosterone may alter this behavior and subsequent pain perception, but the contributions of testosterone are likely subtle and were not detected in this study.