Highly sensitive visual detection of amantadine residues in poultry at the ppb level: A colorimetric immunoassay based on a Fenton reaction and gold nanoparticles aggregation

Colorimetric biosensors for the on-site visual detection of veterinary drug residues are required for food control in developing countries and other resource-constrained areas, where sophisticated instruments may not be available. In this study, we developed a highly sensitive immunoassay for amanta...

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Veröffentlicht in:Analytica chimica acta 2018-10, Vol.1027, p.130-136
Hauptverfasser: Yu, Wenbo, Zhang, Tingting, Ma, Mingfang, Chen, Chaochao, Liang, Xiao, Wen, Kai, Wang, Zhanhui, Shen, Jianzhong
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container_title Analytica chimica acta
container_volume 1027
creator Yu, Wenbo
Zhang, Tingting
Ma, Mingfang
Chen, Chaochao
Liang, Xiao
Wen, Kai
Wang, Zhanhui
Shen, Jianzhong
description Colorimetric biosensors for the on-site visual detection of veterinary drug residues are required for food control in developing countries and other resource-constrained areas, where sophisticated instruments may not be available. In this study, we developed a highly sensitive immunoassay for amantadine residues in poultry. By introducing a novel signal generation strategy into an indirect competitive immunoassay, a highly sensitive assay for amantadine residues in chicken was achieved for naked eye readout at the part per billion (ppb) level. Signal amplification was achieved in the designed immunoassay by combining conventional indirect competitive enzyme-linked immunosorbent assay, Fenton reaction-regulated oxidation of cysteine, and gold nanoparticle aggregation. Therefore, the cascade reaction remarkably enhanced the assay sensitivity and led to a pronounced color change from red to dark purple in the solution, which could be easily distinguished with the naked eye even at approximately 1 μg kg−1 in poultry muscle. Moreover, the color change can be quantitatively assayed with a classic high-throughput plate reader for contaminated poultry samples. The limit of detection (LOD) was 0.51 nM (0.095 ng mL−1). The recovery rates for spiked chicken samples ranged from 78% to 84% with relative standard deviations
doi_str_mv 10.1016/j.aca.2018.04.035
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In this study, we developed a highly sensitive immunoassay for amantadine residues in poultry. By introducing a novel signal generation strategy into an indirect competitive immunoassay, a highly sensitive assay for amantadine residues in chicken was achieved for naked eye readout at the part per billion (ppb) level. Signal amplification was achieved in the designed immunoassay by combining conventional indirect competitive enzyme-linked immunosorbent assay, Fenton reaction-regulated oxidation of cysteine, and gold nanoparticle aggregation. Therefore, the cascade reaction remarkably enhanced the assay sensitivity and led to a pronounced color change from red to dark purple in the solution, which could be easily distinguished with the naked eye even at approximately 1 μg kg−1 in poultry muscle. Moreover, the color change can be quantitatively assayed with a classic high-throughput plate reader for contaminated poultry samples. The limit of detection (LOD) was 0.51 nM (0.095 ng mL−1). The recovery rates for spiked chicken samples ranged from 78% to 84% with relative standard deviations &lt;15%. Therefore, we propose that this immunoassay could be generally applicable for on-site detection in the field of food control. 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In this study, we developed a highly sensitive immunoassay for amantadine residues in poultry. By introducing a novel signal generation strategy into an indirect competitive immunoassay, a highly sensitive assay for amantadine residues in chicken was achieved for naked eye readout at the part per billion (ppb) level. Signal amplification was achieved in the designed immunoassay by combining conventional indirect competitive enzyme-linked immunosorbent assay, Fenton reaction-regulated oxidation of cysteine, and gold nanoparticle aggregation. Therefore, the cascade reaction remarkably enhanced the assay sensitivity and led to a pronounced color change from red to dark purple in the solution, which could be easily distinguished with the naked eye even at approximately 1 μg kg−1 in poultry muscle. Moreover, the color change can be quantitatively assayed with a classic high-throughput plate reader for contaminated poultry samples. The limit of detection (LOD) was 0.51 nM (0.095 ng mL−1). The recovery rates for spiked chicken samples ranged from 78% to 84% with relative standard deviations &lt;15%. Therefore, we propose that this immunoassay could be generally applicable for on-site detection in the field of food control. [Display omitted] •Combining ELISA with Fenton reaction and AuNPs aggregation.•Assay sensitivity remarkably enhanced by a cascade reaction.•Visual and quantitative detection of amantadine in poultry at the ppb level.</abstract><cop>Netherlands</cop><pub>Elsevier B.V</pub><pmid>29866262</pmid><doi>10.1016/j.aca.2018.04.035</doi><tpages>7</tpages><orcidid>https://orcid.org/0000-0002-0167-9559</orcidid><oa>free_for_read</oa></addata></record>
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subjects Agglomeration
Agricultural chemicals
Amantadine
Biosensors
Business competition
Cascade chemical reactions
Chickens
Color
Colorimetry
Control equipment
Developing countries
Drugs
Enzyme-linked immunosorbent assay
Fenton reaction
Food
Gold
Gold nanoparticles
Immunoassay
LDCs
Muscles
Nanoparticles
Oxidation
Poultry
Residues
Sensitivity enhancement
Signal generation
Veterinary drug residues
Veterinary medicine
Visual detection
title Highly sensitive visual detection of amantadine residues in poultry at the ppb level: A colorimetric immunoassay based on a Fenton reaction and gold nanoparticles aggregation
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