Modulation in light utilization by a microalga Asteracys sp. under mixotrophic growth regimes
This study is the first to explore the influence of incident light intensity on the photosynthetic responses under mixotrophic growth of microalga Asteracys sp. When grown mixotrophically, there was an enhanced regulation of non-photochemical quenching (NPQ) of the excited state of chlorophyll (Chl)...
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| Veröffentlicht in: | Photosynthesis research 2019-03, Vol.139 (1-3), p.553-567 |
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| creator | Agarwal, Akanksha Patil, Smita Gharat, Krushna Pandit, Reena A. Lali, Arvind M. |
| description | This study is the first to explore the influence of incident light intensity on the photosynthetic responses under mixotrophic growth of microalga
Asteracys
sp. When grown mixotrophically, there was an enhanced regulation of non-photochemical quenching (NPQ) of the excited state of chlorophyll (Chl)
a
within the cells in response to white cool fluorescent high light (HL; 600 µmol photons m
−2
s
−1
). Simultaneous measurement of reactive oxygen species (ROS) production as malondialdehyde (MDA) and ascorbate peroxidase (APX), an ROS scavenger, showed improved management of stress within mixotrophic cells under HL. Despite the observed decrease in quantum yield of photosynthesis measured through the Chl
a
fluorescence transient, no reduction in biomass accumulation was observed under HL for mixotrophy. However, biomass loss owing to photoinhibition was observed in cells grown phototrophically under the same irradiance. The measurements of dark recovery of NPQ suggested that “state transitions” may be partly responsible for regulating overall photosynthesis in
Asteracys
sp. The partitioning of photochemical and non-photochemical processes to sustain HL stress was analysed. Collectively, this study proposes that mixotrophy using glucose leads to a change in the photosynthetic abilities of
Asteracys
sp. while enhancing the adaptability of the alga to high irradiances.
Graphical Abstract |
| doi_str_mv | 10.1007/s11120-018-0526-8 |
| format | Article |
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Asteracys
sp. When grown mixotrophically, there was an enhanced regulation of non-photochemical quenching (NPQ) of the excited state of chlorophyll (Chl)
a
within the cells in response to white cool fluorescent high light (HL; 600 µmol photons m
−2
s
−1
). Simultaneous measurement of reactive oxygen species (ROS) production as malondialdehyde (MDA) and ascorbate peroxidase (APX), an ROS scavenger, showed improved management of stress within mixotrophic cells under HL. Despite the observed decrease in quantum yield of photosynthesis measured through the Chl
a
fluorescence transient, no reduction in biomass accumulation was observed under HL for mixotrophy. However, biomass loss owing to photoinhibition was observed in cells grown phototrophically under the same irradiance. The measurements of dark recovery of NPQ suggested that “state transitions” may be partly responsible for regulating overall photosynthesis in
Asteracys
sp. The partitioning of photochemical and non-photochemical processes to sustain HL stress was analysed. Collectively, this study proposes that mixotrophy using glucose leads to a change in the photosynthetic abilities of
Asteracys
sp. while enhancing the adaptability of the alga to high irradiances.
Graphical Abstract</description><identifier>ISSN: 0166-8595</identifier><identifier>EISSN: 1573-5079</identifier><identifier>DOI: 10.1007/s11120-018-0526-8</identifier><identifier>PMID: 29860703</identifier><language>eng</language><publisher>Dordrecht: Springer Netherlands</publisher><subject>Adaptability ; Ascorbic acid ; Biochemistry ; Biomass ; Biomedical and Life Sciences ; Chlorophyll ; Fluorescence ; Glucose ; Growth ; L-Ascorbate peroxidase ; Life Sciences ; Light intensity ; Malondialdehyde ; Mixotrophy ; Original Article ; Peroxidase ; Photoinhibition ; Photons ; Photosynthesis ; Plant biochemistry ; Plant Genetics and Genomics ; Plant Physiology ; Plant Sciences ; Reactive oxygen species ; Stress management</subject><ispartof>Photosynthesis research, 2019-03, Vol.139 (1-3), p.553-567</ispartof><rights>Springer Science+Business Media B.V., part of Springer Nature 2018</rights><rights>COPYRIGHT 2019 Springer</rights><rights>Photosynthesis Research is a copyright of Springer, (2018). All Rights Reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c445t-44f34c0bc23bdd60690035d9d9436ee563e4ecc184dd3767508f04e746525a333</citedby><cites>FETCH-LOGICAL-c445t-44f34c0bc23bdd60690035d9d9436ee563e4ecc184dd3767508f04e746525a333</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s11120-018-0526-8$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s11120-018-0526-8$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,776,780,27901,27902,41464,42533,51294</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/29860703$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Agarwal, Akanksha</creatorcontrib><creatorcontrib>Patil, Smita</creatorcontrib><creatorcontrib>Gharat, Krushna</creatorcontrib><creatorcontrib>Pandit, Reena A.</creatorcontrib><creatorcontrib>Lali, Arvind M.</creatorcontrib><title>Modulation in light utilization by a microalga Asteracys sp. under mixotrophic growth regimes</title><title>Photosynthesis research</title><addtitle>Photosynth Res</addtitle><addtitle>Photosynth Res</addtitle><description>This study is the first to explore the influence of incident light intensity on the photosynthetic responses under mixotrophic growth of microalga
Asteracys
sp. When grown mixotrophically, there was an enhanced regulation of non-photochemical quenching (NPQ) of the excited state of chlorophyll (Chl)
a
within the cells in response to white cool fluorescent high light (HL; 600 µmol photons m
−2
s
−1
). Simultaneous measurement of reactive oxygen species (ROS) production as malondialdehyde (MDA) and ascorbate peroxidase (APX), an ROS scavenger, showed improved management of stress within mixotrophic cells under HL. Despite the observed decrease in quantum yield of photosynthesis measured through the Chl
a
fluorescence transient, no reduction in biomass accumulation was observed under HL for mixotrophy. However, biomass loss owing to photoinhibition was observed in cells grown phototrophically under the same irradiance. The measurements of dark recovery of NPQ suggested that “state transitions” may be partly responsible for regulating overall photosynthesis in
Asteracys
sp. The partitioning of photochemical and non-photochemical processes to sustain HL stress was analysed. Collectively, this study proposes that mixotrophy using glucose leads to a change in the photosynthetic abilities of
Asteracys
sp. while enhancing the adaptability of the alga to high irradiances.
Graphical Abstract</description><subject>Adaptability</subject><subject>Ascorbic acid</subject><subject>Biochemistry</subject><subject>Biomass</subject><subject>Biomedical and Life Sciences</subject><subject>Chlorophyll</subject><subject>Fluorescence</subject><subject>Glucose</subject><subject>Growth</subject><subject>L-Ascorbate peroxidase</subject><subject>Life Sciences</subject><subject>Light intensity</subject><subject>Malondialdehyde</subject><subject>Mixotrophy</subject><subject>Original Article</subject><subject>Peroxidase</subject><subject>Photoinhibition</subject><subject>Photons</subject><subject>Photosynthesis</subject><subject>Plant biochemistry</subject><subject>Plant Genetics and Genomics</subject><subject>Plant Physiology</subject><subject>Plant Sciences</subject><subject>Reactive oxygen species</subject><subject>Stress management</subject><issn>0166-8595</issn><issn>1573-5079</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2019</creationdate><recordtype>article</recordtype><sourceid>BENPR</sourceid><recordid>eNp1kVuLFDEQhYMo7rj6A3yRgC_60GOlc-n047B4WVgRvDxKyCTVPVl6OmPSze74683Qq7KC5CFQ9Z2i6hxCnjNYM4DmTWaM1VAB0xXIWlX6AVkx2fBKQtM-JCtgqhRlK8_Ik5yvAUArxh-Ts7rVChrgK_L9Y_TzYKcQRxpGOoR-N9F5CkP4uRS3R2rpPrgU7dBbuskTJuuOmebDms6jx1S6t3FK8bALjvYp3kw7mrAPe8xPyaPODhmf3f3n5Nu7t18vPlRXn95fXmyuKieEnCohOi4cbF3Nt94rUC0Al771reAKUSqOAp1jWnjPG9VI0B0IbISStbSc83Pyapl7SPHHjHky-5AdDoMdMc7Z1CDaVkjW1gV9-Q96Hec0lu1OlNas4ZwVar1QvR3QhLErB1pXnsfiRRyxC6W-kU1xUtdKFMHre4LCTHg79XbO2Vx--XyfZQtbTM05YWcOKextOhoG5hSsWYI1JVhzCtboonlxt_a83aP_o_idZAHqBcilNfaY_t71_6m_AHozq8s</recordid><startdate>20190301</startdate><enddate>20190301</enddate><creator>Agarwal, Akanksha</creator><creator>Patil, Smita</creator><creator>Gharat, Krushna</creator><creator>Pandit, Reena A.</creator><creator>Lali, Arvind M.</creator><general>Springer Netherlands</general><general>Springer</general><general>Springer Nature B.V</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>ISR</scope><scope>3V.</scope><scope>7QP</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>88I</scope><scope>8AO</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M2P</scope><scope>M7N</scope><scope>M7P</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>Q9U</scope><scope>7X8</scope></search><sort><creationdate>20190301</creationdate><title>Modulation in light utilization by a microalga Asteracys sp. under mixotrophic growth regimes</title><author>Agarwal, Akanksha ; Patil, Smita ; Gharat, Krushna ; Pandit, Reena A. ; Lali, Arvind M.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c445t-44f34c0bc23bdd60690035d9d9436ee563e4ecc184dd3767508f04e746525a333</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2019</creationdate><topic>Adaptability</topic><topic>Ascorbic acid</topic><topic>Biochemistry</topic><topic>Biomass</topic><topic>Biomedical and Life Sciences</topic><topic>Chlorophyll</topic><topic>Fluorescence</topic><topic>Glucose</topic><topic>Growth</topic><topic>L-Ascorbate peroxidase</topic><topic>Life Sciences</topic><topic>Light intensity</topic><topic>Malondialdehyde</topic><topic>Mixotrophy</topic><topic>Original Article</topic><topic>Peroxidase</topic><topic>Photoinhibition</topic><topic>Photons</topic><topic>Photosynthesis</topic><topic>Plant biochemistry</topic><topic>Plant Genetics and Genomics</topic><topic>Plant Physiology</topic><topic>Plant Sciences</topic><topic>Reactive oxygen species</topic><topic>Stress management</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Agarwal, Akanksha</creatorcontrib><creatorcontrib>Patil, Smita</creatorcontrib><creatorcontrib>Gharat, Krushna</creatorcontrib><creatorcontrib>Pandit, Reena A.</creatorcontrib><creatorcontrib>Lali, Arvind M.</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>Gale In Context: Science</collection><collection>ProQuest Central (Corporate)</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Science Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Science Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biological Science Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>ProQuest Central Basic</collection><collection>MEDLINE - Academic</collection><jtitle>Photosynthesis research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Agarwal, Akanksha</au><au>Patil, Smita</au><au>Gharat, Krushna</au><au>Pandit, Reena A.</au><au>Lali, Arvind M.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Modulation in light utilization by a microalga Asteracys sp. under mixotrophic growth regimes</atitle><jtitle>Photosynthesis research</jtitle><stitle>Photosynth Res</stitle><addtitle>Photosynth Res</addtitle><date>2019-03-01</date><risdate>2019</risdate><volume>139</volume><issue>1-3</issue><spage>553</spage><epage>567</epage><pages>553-567</pages><issn>0166-8595</issn><eissn>1573-5079</eissn><abstract>This study is the first to explore the influence of incident light intensity on the photosynthetic responses under mixotrophic growth of microalga
Asteracys
sp. When grown mixotrophically, there was an enhanced regulation of non-photochemical quenching (NPQ) of the excited state of chlorophyll (Chl)
a
within the cells in response to white cool fluorescent high light (HL; 600 µmol photons m
−2
s
−1
). Simultaneous measurement of reactive oxygen species (ROS) production as malondialdehyde (MDA) and ascorbate peroxidase (APX), an ROS scavenger, showed improved management of stress within mixotrophic cells under HL. Despite the observed decrease in quantum yield of photosynthesis measured through the Chl
a
fluorescence transient, no reduction in biomass accumulation was observed under HL for mixotrophy. However, biomass loss owing to photoinhibition was observed in cells grown phototrophically under the same irradiance. The measurements of dark recovery of NPQ suggested that “state transitions” may be partly responsible for regulating overall photosynthesis in
Asteracys
sp. The partitioning of photochemical and non-photochemical processes to sustain HL stress was analysed. Collectively, this study proposes that mixotrophy using glucose leads to a change in the photosynthetic abilities of
Asteracys
sp. while enhancing the adaptability of the alga to high irradiances.
Graphical Abstract</abstract><cop>Dordrecht</cop><pub>Springer Netherlands</pub><pmid>29860703</pmid><doi>10.1007/s11120-018-0526-8</doi><tpages>15</tpages></addata></record> |
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| ispartof | Photosynthesis research, 2019-03, Vol.139 (1-3), p.553-567 |
| issn | 0166-8595 1573-5079 |
| language | eng |
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| source | Springer Nature - Complete Springer Journals |
| subjects | Adaptability Ascorbic acid Biochemistry Biomass Biomedical and Life Sciences Chlorophyll Fluorescence Glucose Growth L-Ascorbate peroxidase Life Sciences Light intensity Malondialdehyde Mixotrophy Original Article Peroxidase Photoinhibition Photons Photosynthesis Plant biochemistry Plant Genetics and Genomics Plant Physiology Plant Sciences Reactive oxygen species Stress management |
| title | Modulation in light utilization by a microalga Asteracys sp. under mixotrophic growth regimes |
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