Preparation and protective efficacy of a chicken embryo kidney cell-attenuation GI-19/QX-like avian infectious bronchitis virus vaccine
•A CEK-attenuated QX-like IBV vaccine SczyC100 was prepared.•SczyC100 is effective against prevalent Chinese IBV strains.•The vaccine efficacy of SczyC100 is significantly better than H120. Avian infectious bronchitis (IB) is a highly contagious disease, and hazardous to the poultry industry. Immune...
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| Veröffentlicht in: | Vaccine 2018-06, Vol.36 (28), p.4087-4094 |
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| container_title | Vaccine |
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| creator | Xia, Jing He, Xiao Du, Li-Jing Liu, Yue-Yue You, Guo-Jin Li, Shu-Yun Liu, Ping Cao, San-Jie Han, Xin-Feng Huang, Yong |
| description | •A CEK-attenuated QX-like IBV vaccine SczyC100 was prepared.•SczyC100 is effective against prevalent Chinese IBV strains.•The vaccine efficacy of SczyC100 is significantly better than H120.
Avian infectious bronchitis (IB) is a highly contagious disease, and hazardous to the poultry industry. Immune failure often occurs due to the emergence of new serotypes or field strains antigenically different from the vaccine strains. To prepare a candidate vaccine against the prevalent avian infectious bronchitis virus (IBV) in China, the GI-19/QX-like field isolate Sczy3 was selected as the progenitor strain and attenuated via passaging in chicken embryo kidney (CEK) cells for 100 times. The 100th generation of CEK-adapted strain, designated SczyC100, was safe to use on one-day old specific pathogen-free (SPF) chicken as determined by pathogenicity and virulence reversion test. The efficacies of SczyC100 and two commonly used commercial vaccines (H120 and 4/91) against prevalent GI-19/QX and GI-7/TWI type virulent strains were evaluated. Sczy3C100 effectively reduced the morbidity, mortality, mean lesion scores (MLSs), and viral load of trachea of chickens challenged by GI-19/QX and GI-7/TWI strains. CEK-adapted SczyC100 is therefore a potential vaccine candidate for the control of IB in China. |
| doi_str_mv | 10.1016/j.vaccine.2018.05.094 |
| format | Article |
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Avian infectious bronchitis (IB) is a highly contagious disease, and hazardous to the poultry industry. Immune failure often occurs due to the emergence of new serotypes or field strains antigenically different from the vaccine strains. To prepare a candidate vaccine against the prevalent avian infectious bronchitis virus (IBV) in China, the GI-19/QX-like field isolate Sczy3 was selected as the progenitor strain and attenuated via passaging in chicken embryo kidney (CEK) cells for 100 times. The 100th generation of CEK-adapted strain, designated SczyC100, was safe to use on one-day old specific pathogen-free (SPF) chicken as determined by pathogenicity and virulence reversion test. The efficacies of SczyC100 and two commonly used commercial vaccines (H120 and 4/91) against prevalent GI-19/QX and GI-7/TWI type virulent strains were evaluated. Sczy3C100 effectively reduced the morbidity, mortality, mean lesion scores (MLSs), and viral load of trachea of chickens challenged by GI-19/QX and GI-7/TWI strains. CEK-adapted SczyC100 is therefore a potential vaccine candidate for the control of IB in China.</description><identifier>ISSN: 0264-410X</identifier><identifier>EISSN: 1873-2518</identifier><identifier>DOI: 10.1016/j.vaccine.2018.05.094</identifier><identifier>PMID: 29859801</identifier><language>eng</language><publisher>Netherlands: Elsevier Ltd</publisher><subject><![CDATA[Animals ; Attenuation ; Avian infectious bronchitis ; Bronchitis ; CEK adaptation ; Cell Line ; Chickens ; China ; Coronavirus Infections - immunology ; Coronavirus Infections - pathology ; Coronavirus Infections - prevention & control ; Coronavirus Infections - veterinary ; Design ; Disease ; Eggs ; Embryos ; Epithelial Cells - virology ; Infections ; Infectious bronchitis disease ; Infectious bronchitis virus - growth & development ; Infectious bronchitis virus - immunology ; Infectious bronchitis virus - pathogenicity ; Influenza ; Kidneys ; Laboratory animals ; Morbidity ; Mortality ; Pathogenicity ; Pathogens ; Poultry ; Poultry Diseases - immunology ; Poultry Diseases - prevention & control ; Protective ; Reversion ; RNA polymerase ; Safety ; Serial Passage ; Serotypes ; Strains (organisms) ; Survival Analysis ; Thermal cycling ; Trachea ; Trachea - virology ; Vaccine ; Vaccines ; Vaccines, Attenuated - administration & dosage ; Vaccines, Attenuated - immunology ; Vaccines, Attenuated - isolation & purification ; Veterinary medicine ; Viral Load ; Viral Vaccines - administration & dosage ; Viral Vaccines - immunology ; Viral Vaccines - isolation & purification ; Virulence ; Virus Cultivation - methods ; Viruses]]></subject><ispartof>Vaccine, 2018-06, Vol.36 (28), p.4087-4094</ispartof><rights>2018 Elsevier Ltd</rights><rights>Copyright © 2018 Elsevier Ltd. All rights reserved.</rights><rights>2018. Elsevier Ltd</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c393t-a4df0549f4bd40575a6c79bc93195a5214682b61240318f7cbb80d21818ec56d3</citedby><cites>FETCH-LOGICAL-c393t-a4df0549f4bd40575a6c79bc93195a5214682b61240318f7cbb80d21818ec56d3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0264410X18307606$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/29859801$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Xia, Jing</creatorcontrib><creatorcontrib>He, Xiao</creatorcontrib><creatorcontrib>Du, Li-Jing</creatorcontrib><creatorcontrib>Liu, Yue-Yue</creatorcontrib><creatorcontrib>You, Guo-Jin</creatorcontrib><creatorcontrib>Li, Shu-Yun</creatorcontrib><creatorcontrib>Liu, Ping</creatorcontrib><creatorcontrib>Cao, San-Jie</creatorcontrib><creatorcontrib>Han, Xin-Feng</creatorcontrib><creatorcontrib>Huang, Yong</creatorcontrib><title>Preparation and protective efficacy of a chicken embryo kidney cell-attenuation GI-19/QX-like avian infectious bronchitis virus vaccine</title><title>Vaccine</title><addtitle>Vaccine</addtitle><description>•A CEK-attenuated QX-like IBV vaccine SczyC100 was prepared.•SczyC100 is effective against prevalent Chinese IBV strains.•The vaccine efficacy of SczyC100 is significantly better than H120.
Avian infectious bronchitis (IB) is a highly contagious disease, and hazardous to the poultry industry. Immune failure often occurs due to the emergence of new serotypes or field strains antigenically different from the vaccine strains. To prepare a candidate vaccine against the prevalent avian infectious bronchitis virus (IBV) in China, the GI-19/QX-like field isolate Sczy3 was selected as the progenitor strain and attenuated via passaging in chicken embryo kidney (CEK) cells for 100 times. The 100th generation of CEK-adapted strain, designated SczyC100, was safe to use on one-day old specific pathogen-free (SPF) chicken as determined by pathogenicity and virulence reversion test. The efficacies of SczyC100 and two commonly used commercial vaccines (H120 and 4/91) against prevalent GI-19/QX and GI-7/TWI type virulent strains were evaluated. Sczy3C100 effectively reduced the morbidity, mortality, mean lesion scores (MLSs), and viral load of trachea of chickens challenged by GI-19/QX and GI-7/TWI strains. CEK-adapted SczyC100 is therefore a potential vaccine candidate for the control of IB in China.</description><subject>Animals</subject><subject>Attenuation</subject><subject>Avian infectious bronchitis</subject><subject>Bronchitis</subject><subject>CEK adaptation</subject><subject>Cell Line</subject><subject>Chickens</subject><subject>China</subject><subject>Coronavirus Infections - immunology</subject><subject>Coronavirus Infections - pathology</subject><subject>Coronavirus Infections - prevention & control</subject><subject>Coronavirus Infections - veterinary</subject><subject>Design</subject><subject>Disease</subject><subject>Eggs</subject><subject>Embryos</subject><subject>Epithelial Cells - virology</subject><subject>Infections</subject><subject>Infectious bronchitis disease</subject><subject>Infectious bronchitis virus - growth & development</subject><subject>Infectious bronchitis virus - immunology</subject><subject>Infectious bronchitis virus - pathogenicity</subject><subject>Influenza</subject><subject>Kidneys</subject><subject>Laboratory animals</subject><subject>Morbidity</subject><subject>Mortality</subject><subject>Pathogenicity</subject><subject>Pathogens</subject><subject>Poultry</subject><subject>Poultry Diseases - immunology</subject><subject>Poultry Diseases - prevention & control</subject><subject>Protective</subject><subject>Reversion</subject><subject>RNA polymerase</subject><subject>Safety</subject><subject>Serial Passage</subject><subject>Serotypes</subject><subject>Strains (organisms)</subject><subject>Survival Analysis</subject><subject>Thermal cycling</subject><subject>Trachea</subject><subject>Trachea - virology</subject><subject>Vaccine</subject><subject>Vaccines</subject><subject>Vaccines, Attenuated - administration & dosage</subject><subject>Vaccines, Attenuated - immunology</subject><subject>Vaccines, Attenuated - isolation & purification</subject><subject>Veterinary medicine</subject><subject>Viral Load</subject><subject>Viral Vaccines - administration & dosage</subject><subject>Viral Vaccines - immunology</subject><subject>Viral Vaccines - isolation & purification</subject><subject>Virulence</subject><subject>Virus Cultivation - methods</subject><subject>Viruses</subject><issn>0264-410X</issn><issn>1873-2518</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2018</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>8G5</sourceid><sourceid>BENPR</sourceid><sourceid>GUQSH</sourceid><sourceid>M2O</sourceid><recordid>eNqFkc2KFDEUhYOMOO3oIyiB2bipmtxUUp2sRJpxZmBABYXZhVRyC9M_qTapKugn8LVN0z0u3LgKXL57cs85hLwDVgOD9mZdz9a5ELHmDFTNZM20eEEWoJZNxSWoC7JgvBWVAPZ0SV7nvGaMyQb0K3LJtZJaMViQ318T7m2yYxgitdHTfRpGdGOYkWLfB2fdgQ49tdT9DG6DkeKuS4eBboKPeKAOt9vKjiPG6aRx91CBvvn2VG3DBqmdg400xP4oOUyZdmmIRWkMmc4hlcHZxRvysrfbjG_P7xX58fn2--q-evxy97D69Fi5RjdjZYXvmRS6F50XTC6lbd1Sd04XX9JKDqJVvGuBC9aA6peu6xTzHBQodLL1zRX5cNItPn9NmEezC_lowkYs9xnOhNaN4NAW9PofdD1MKZbrCiVBlgRbKJQ8US4NOSfszT6FnU0HA8wcmzJrc_Zojk0ZJk1pquy9P6tP3Q79363nagrw8QRgiWMOmEx2AaNDH1JJ0_gh_OeLP4U5p8Y</recordid><startdate>20180627</startdate><enddate>20180627</enddate><creator>Xia, Jing</creator><creator>He, Xiao</creator><creator>Du, Li-Jing</creator><creator>Liu, Yue-Yue</creator><creator>You, Guo-Jin</creator><creator>Li, Shu-Yun</creator><creator>Liu, Ping</creator><creator>Cao, San-Jie</creator><creator>Han, Xin-Feng</creator><creator>Huang, Yong</creator><general>Elsevier Ltd</general><general>Elsevier Limited</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QL</scope><scope>7RV</scope><scope>7T2</scope><scope>7T5</scope><scope>7U9</scope><scope>7X7</scope><scope>7XB</scope><scope>88C</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>8G5</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>GUQSH</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9-</scope><scope>K9.</scope><scope>KB0</scope><scope>LK8</scope><scope>M0R</scope><scope>M0S</scope><scope>M0T</scope><scope>M1P</scope><scope>M2O</scope><scope>M7N</scope><scope>M7P</scope><scope>MBDVC</scope><scope>NAPCQ</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>Q9U</scope><scope>7X8</scope></search><sort><creationdate>20180627</creationdate><title>Preparation and protective efficacy of a chicken embryo kidney cell-attenuation GI-19/QX-like avian infectious bronchitis virus vaccine</title><author>Xia, Jing ; He, Xiao ; Du, Li-Jing ; Liu, Yue-Yue ; You, Guo-Jin ; Li, Shu-Yun ; Liu, Ping ; Cao, San-Jie ; Han, Xin-Feng ; Huang, Yong</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c393t-a4df0549f4bd40575a6c79bc93195a5214682b61240318f7cbb80d21818ec56d3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2018</creationdate><topic>Animals</topic><topic>Attenuation</topic><topic>Avian infectious bronchitis</topic><topic>Bronchitis</topic><topic>CEK adaptation</topic><topic>Cell Line</topic><topic>Chickens</topic><topic>China</topic><topic>Coronavirus Infections - immunology</topic><topic>Coronavirus Infections - pathology</topic><topic>Coronavirus Infections - prevention & control</topic><topic>Coronavirus Infections - veterinary</topic><topic>Design</topic><topic>Disease</topic><topic>Eggs</topic><topic>Embryos</topic><topic>Epithelial Cells - virology</topic><topic>Infections</topic><topic>Infectious bronchitis disease</topic><topic>Infectious bronchitis virus - growth & development</topic><topic>Infectious bronchitis virus - immunology</topic><topic>Infectious bronchitis virus - pathogenicity</topic><topic>Influenza</topic><topic>Kidneys</topic><topic>Laboratory animals</topic><topic>Morbidity</topic><topic>Mortality</topic><topic>Pathogenicity</topic><topic>Pathogens</topic><topic>Poultry</topic><topic>Poultry Diseases - immunology</topic><topic>Poultry Diseases - prevention & control</topic><topic>Protective</topic><topic>Reversion</topic><topic>RNA polymerase</topic><topic>Safety</topic><topic>Serial Passage</topic><topic>Serotypes</topic><topic>Strains (organisms)</topic><topic>Survival Analysis</topic><topic>Thermal cycling</topic><topic>Trachea</topic><topic>Trachea - virology</topic><topic>Vaccine</topic><topic>Vaccines</topic><topic>Vaccines, Attenuated - administration & dosage</topic><topic>Vaccines, Attenuated - immunology</topic><topic>Vaccines, Attenuated - isolation & purification</topic><topic>Veterinary medicine</topic><topic>Viral Load</topic><topic>Viral Vaccines - administration & dosage</topic><topic>Viral Vaccines - immunology</topic><topic>Viral Vaccines - isolation & purification</topic><topic>Virulence</topic><topic>Virus Cultivation - methods</topic><topic>Viruses</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Xia, Jing</creatorcontrib><creatorcontrib>He, Xiao</creatorcontrib><creatorcontrib>Du, Li-Jing</creatorcontrib><creatorcontrib>Liu, Yue-Yue</creatorcontrib><creatorcontrib>You, Guo-Jin</creatorcontrib><creatorcontrib>Li, Shu-Yun</creatorcontrib><creatorcontrib>Liu, Ping</creatorcontrib><creatorcontrib>Cao, San-Jie</creatorcontrib><creatorcontrib>Han, Xin-Feng</creatorcontrib><creatorcontrib>Huang, Yong</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Nursing & Allied Health Database</collection><collection>Health and Safety Science Abstracts (Full archive)</collection><collection>Immunology Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Healthcare Administration Database (Alumni)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Public Health Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Research Library (Alumni Edition)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>Research Library Prep</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>Consumer Health Database (Alumni Edition)</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Nursing & Allied Health Database (Alumni Edition)</collection><collection>ProQuest Biological Science Collection</collection><collection>Consumer Health Database</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Healthcare Administration Database</collection><collection>Medical Database</collection><collection>Research Library</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biological Science Database</collection><collection>Research Library (Corporate)</collection><collection>Nursing & Allied Health Premium</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central Basic</collection><collection>MEDLINE - Academic</collection><jtitle>Vaccine</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Xia, Jing</au><au>He, Xiao</au><au>Du, Li-Jing</au><au>Liu, Yue-Yue</au><au>You, Guo-Jin</au><au>Li, Shu-Yun</au><au>Liu, Ping</au><au>Cao, San-Jie</au><au>Han, Xin-Feng</au><au>Huang, Yong</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Preparation and protective efficacy of a chicken embryo kidney cell-attenuation GI-19/QX-like avian infectious bronchitis virus vaccine</atitle><jtitle>Vaccine</jtitle><addtitle>Vaccine</addtitle><date>2018-06-27</date><risdate>2018</risdate><volume>36</volume><issue>28</issue><spage>4087</spage><epage>4094</epage><pages>4087-4094</pages><issn>0264-410X</issn><eissn>1873-2518</eissn><abstract>•A CEK-attenuated QX-like IBV vaccine SczyC100 was prepared.•SczyC100 is effective against prevalent Chinese IBV strains.•The vaccine efficacy of SczyC100 is significantly better than H120.
Avian infectious bronchitis (IB) is a highly contagious disease, and hazardous to the poultry industry. Immune failure often occurs due to the emergence of new serotypes or field strains antigenically different from the vaccine strains. To prepare a candidate vaccine against the prevalent avian infectious bronchitis virus (IBV) in China, the GI-19/QX-like field isolate Sczy3 was selected as the progenitor strain and attenuated via passaging in chicken embryo kidney (CEK) cells for 100 times. The 100th generation of CEK-adapted strain, designated SczyC100, was safe to use on one-day old specific pathogen-free (SPF) chicken as determined by pathogenicity and virulence reversion test. The efficacies of SczyC100 and two commonly used commercial vaccines (H120 and 4/91) against prevalent GI-19/QX and GI-7/TWI type virulent strains were evaluated. Sczy3C100 effectively reduced the morbidity, mortality, mean lesion scores (MLSs), and viral load of trachea of chickens challenged by GI-19/QX and GI-7/TWI strains. CEK-adapted SczyC100 is therefore a potential vaccine candidate for the control of IB in China.</abstract><cop>Netherlands</cop><pub>Elsevier Ltd</pub><pmid>29859801</pmid><doi>10.1016/j.vaccine.2018.05.094</doi><tpages>8</tpages></addata></record> |
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| subjects | Animals Attenuation Avian infectious bronchitis Bronchitis CEK adaptation Cell Line Chickens China Coronavirus Infections - immunology Coronavirus Infections - pathology Coronavirus Infections - prevention & control Coronavirus Infections - veterinary Design Disease Eggs Embryos Epithelial Cells - virology Infections Infectious bronchitis disease Infectious bronchitis virus - growth & development Infectious bronchitis virus - immunology Infectious bronchitis virus - pathogenicity Influenza Kidneys Laboratory animals Morbidity Mortality Pathogenicity Pathogens Poultry Poultry Diseases - immunology Poultry Diseases - prevention & control Protective Reversion RNA polymerase Safety Serial Passage Serotypes Strains (organisms) Survival Analysis Thermal cycling Trachea Trachea - virology Vaccine Vaccines Vaccines, Attenuated - administration & dosage Vaccines, Attenuated - immunology Vaccines, Attenuated - isolation & purification Veterinary medicine Viral Load Viral Vaccines - administration & dosage Viral Vaccines - immunology Viral Vaccines - isolation & purification Virulence Virus Cultivation - methods Viruses |
| title | Preparation and protective efficacy of a chicken embryo kidney cell-attenuation GI-19/QX-like avian infectious bronchitis virus vaccine |
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