Cells over-expressing EAAT2 protect motoneurons from excitotoxic death in vitro
Amyotrophic lateral sclerosis is an incurable disease in which cerebral and spinal motoneurons degenerate, causing paralysis and death within 2–5 years. One of the pathogenic factors of motoneuron death is a chronic excess of glutamate, which exceeds its removal by astrocytes, i.e. excitotoxicity. E...
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| Veröffentlicht in: | Neuroreport 2003-10, Vol.14 (15), p.1967-1970 |
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| container_end_page | 1970 |
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| container_issue | 15 |
| container_start_page | 1967 |
| container_title | Neuroreport |
| container_volume | 14 |
| creator | Wisman, Liselijn A. B van Muiswinkel, Freek L de Graan, Pierre N. E Hol, Elly M Bär, Peter R |
| description | Amyotrophic lateral sclerosis is an incurable disease in which cerebral and spinal motoneurons degenerate, causing paralysis and death within 2–5 years. One of the pathogenic factors of motoneuron death is a chronic excess of glutamate, which exceeds its removal by astrocytes, i.e. excitotoxicity. Extra glutamate uptake in the spinal cord may slow down or prevent motoneuron death. We have engineered cells over-expressing the main glutamate transporter and tested their potential to rescue motoneurons exposed to high levels of glutamate in vitro. The engineered cells protected motoneurons in a motoneuron–astrocyte co-culture at glutamate concentrations when astrocytes were no longer capable of removing glutamate. This suggests that engineered cells, introduced into the spinal column, can help remove glutamate, thereby preventing motoneuron death. |
| doi_str_mv | 10.1097/00001756-200310270-00017 |
| format | Article |
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Psychology</subject><subject>Genetic Engineering</subject><subject>Glutamic Acid - metabolism</subject><subject>Glutamic Acid - toxicity</subject><subject>Humans</subject><subject>Motor Neurons - physiology</subject><subject>Neurotransmitter Uptake Inhibitors - pharmacology</subject><subject>Pyrrolidines - pharmacology</subject><subject>Rats</subject><subject>Rats, Sprague-Dawley</subject><subject>Vertebrates: nervous system and sense organs</subject><issn>0959-4965</issn><issn>1473-558X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2003</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1kclOwzAQhi0EgrK8AvIFbgGvcXysqrJISFxA4mYZZ0IDSVxspy1vT7oAJ-Yy0q9vtn8QwpRcUaLVNRmCKplnjBBOCVMk2yh7aESF4pmUxcs-GhEtdSZ0Lo_QcYzvA6IJLQ7RERUyp5qTEXqcQNNE7BcQMljNA8RYd294Oh4_MTwPPoFLuPXJd9AH30VcBd9iWLk6DeKqdrgEm2a47vCiTsGfooPKNhHOdvkEPd9MnyZ32cPj7f1k_JA5QZXKKmq5ZoWlXFAmhFTWcWUJE-xVlJxaB5KVSlkpKksqB8Tm4KRmoMuS50ryE3S57Tvs-NlDTKatoxtusR34PhpGhC6EZgNYbEEXfIwBKjMPdWvDl6HErM00P2aaXzPNRhlKz3cz-tcWyr_CnXsDcLEDbHS2qYLtXB3_OCllXtD1smLLLX2TIMSPpl9CMDOwTZqZ_57JvwGgc4vN</recordid><startdate>20031027</startdate><enddate>20031027</enddate><creator>Wisman, Liselijn A. 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Psychology</topic><topic>Genetic Engineering</topic><topic>Glutamic Acid - metabolism</topic><topic>Glutamic Acid - toxicity</topic><topic>Humans</topic><topic>Motor Neurons - physiology</topic><topic>Neurotransmitter Uptake Inhibitors - pharmacology</topic><topic>Pyrrolidines - pharmacology</topic><topic>Rats</topic><topic>Rats, Sprague-Dawley</topic><topic>Vertebrates: nervous system and sense organs</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Wisman, Liselijn A. B</creatorcontrib><creatorcontrib>van Muiswinkel, Freek L</creatorcontrib><creatorcontrib>de Graan, Pierre N. 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| ispartof | Neuroreport, 2003-10, Vol.14 (15), p.1967-1970 |
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| source | MEDLINE; Journals@Ovid Complete |
| subjects | Animals Astrocytes - drug effects Astrocytes - metabolism Biological and medical sciences Blotting, Western Cell Communication - genetics Cell Death - drug effects Cell Death - genetics Coculture Techniques Dicarboxylic Acids - pharmacology Fundamental and applied biological sciences. Psychology Genetic Engineering Glutamic Acid - metabolism Glutamic Acid - toxicity Humans Motor Neurons - physiology Neurotransmitter Uptake Inhibitors - pharmacology Pyrrolidines - pharmacology Rats Rats, Sprague-Dawley Vertebrates: nervous system and sense organs |
| title | Cells over-expressing EAAT2 protect motoneurons from excitotoxic death in vitro |
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