Salinivibrio siamensis sp. nov., from fermented fish (pla-ra) in Thailand

1 Department of Microbiology, Faculty of Pharmaceutical Sciences, Chulalongkorn University, Bangkok 10330, Thailand 2 Department of Microbiology, Faculty of Science, Chulalongkorn University, Bangkok 10330, Thailand 3 National Center for Genetic Engineering and Biotechnology, Pathumthani 12120, Thailand 4 Japan Collection of Microorganisms, RIKEN BioResource Center, 2-1 Hirosawa, Wako-shi, Saitama 351-0198, Japan Correspondence Somboon Tanasupawat Somboon.T{at}chula.ac.th A Gram-negative, facultatively anaerobic, moderately halophilic bacterium, strain ND1-1 T , was isolated from fermented fish ( pla-ra ) in Thailand. The cells were curved rods, motile and non-endospore-forming. The novel strain grew optimally at 37 °C, at pH 8 and in the presence of 9–10 % (w/v) NaCl. The predominant respiratory lipoquinone was Q-8. The major cellular fatty acids were C 16 : 0 and C 12 : 0 . Polar lipid analysis revealed the presence of phosphatidylethanolamine, phosphatidylglycerol and diphosphatidylglycerol. The DNA G+C content was 49.0 mol%. Comparative 16S rRNA gene sequence analyses indicated that strain ND1-1 T was closely related to Salinivibrio costicola , which comprises three subspecies, and Salinivibrio proteolyticus with gene sequence similarities of 98.3–98.6 %. Strain ND1-1 T showed low levels of DNA–DNA relatedness with S. costicola subsp. costicola JCM 15095 T (33.2 %), S. costicola subsp. alcaliphilus DSM 16359 T (38.4 %), S. costicola subsp. vallismortis JCM 15096 T (59.7 %), and S. proteolyticus AF-2004 T (42.1 %). On the basis of the physiological and biochemical characteristics and the molecular data presented, strain ND1-1 T should be classified as a novel species of the genus Salinivibrio for which the name Salinivibrio siamensis sp. nov. is proposed. The type strain is ND1-1 T (=JCM 14472 T =PCU 301 T =TISTR 1810 T ). Abbreviations: DPG, diphosphatidylglycerol; PE, phosphatidylethanolamine; PG, phosphatidylglycerol The GenBank/EMBL/DDBJ accession number for the 16S rRNA gene sequence of strain ND1-1 T is AB285018 . Additional phylogenetic trees constructed by the maximum-parsimony and maximum-likelihood methods are available with the online version of this paper.