Sandwich fusion of CBM9_2 to enhance xylanase thermostability and activity

Used as model for sandwich fusion, a mesophilic Aspergillus niger GH11 xylanase (Xyn) was fused into C2-Xyn-C2 with a thermophilic Thermotaga maritima GH10 xylanase carbohydrate-binding module CBM9_2 (C2). Linearized plasmids C2-pET20b-C2-Xyn were amplified from template pET20b-Xyn-C2 with a 4.3 kb...

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Veröffentlicht in:	International journal of biological macromolecules 2018-10, Vol.117, p.586-591
Hauptverfasser:	Yang, Ang, Cheng, Jinsheng, Liu, Meng, Shangguan, Yunjie, Liu, Liangwei
Format:	Artikel
Sprache:	eng
Schlagworte:	Activity Sandwich fusion Thermostability Xylanase
Online-Zugang:	Volltext
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