Acylphosphatase expression during macrophage differentiation and activation of U-937 cell line
The two acylphosphatase isoenzymes (muscle type and common type) are differently involved in cell differentiation processes. In this paper we investigate the expression of the two isoenzymes during macrophage differentiation and activation. The U-937 human promonocytic cell line is a model for cell...
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| Veröffentlicht in: | Biochimie 1999-11, Vol.81 (11), p.1031-1035 |
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| creator | Degl'Innocenti, Donatella Marzocchini, Riccardo Rosati, Fabiana Cellini, Elena Raugei, Giovanni Ramponi, Giampietro |
| description | The two acylphosphatase isoenzymes (muscle type and common type) are differently involved in cell differentiation processes. In this paper we investigate the expression of the two isoenzymes during macrophage differentiation and activation. The U-937 human promonocytic cell line is a model for cell differentiation induced by the tumor promoter phorbol myristic acetate (PMA). Here we show that only the expression of the muscle type acylphosphatase increases during U-937 differentiation and macrophage activation, confirming that the two isoenzymes are differently regulated. Moreover, we determined, in the same conditions, the level of specific mRNA. Results show that after an initial two-fold decrease during PMA stimulation, the muscle type acylphosphatase mRNA levels remain constant also after the treatment with lipopolysaccharide and γ-interferon, treatments that lead to macrophage activation. It is possible that post-transcription regulation is responsible for the regulation of muscle type acylphosphatase in the cell during differentiation and macrophage activation. |
| doi_str_mv | 10.1016/S0300-9084(99)00333-8 |
| format | Article |
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In this paper we investigate the expression of the two isoenzymes during macrophage differentiation and activation. The U-937 human promonocytic cell line is a model for cell differentiation induced by the tumor promoter phorbol myristic acetate (PMA). Here we show that only the expression of the muscle type acylphosphatase increases during U-937 differentiation and macrophage activation, confirming that the two isoenzymes are differently regulated. Moreover, we determined, in the same conditions, the level of specific mRNA. Results show that after an initial two-fold decrease during PMA stimulation, the muscle type acylphosphatase mRNA levels remain constant also after the treatment with lipopolysaccharide and γ-interferon, treatments that lead to macrophage activation. 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In this paper we investigate the expression of the two isoenzymes during macrophage differentiation and activation. The U-937 human promonocytic cell line is a model for cell differentiation induced by the tumor promoter phorbol myristic acetate (PMA). Here we show that only the expression of the muscle type acylphosphatase increases during U-937 differentiation and macrophage activation, confirming that the two isoenzymes are differently regulated. Moreover, we determined, in the same conditions, the level of specific mRNA. Results show that after an initial two-fold decrease during PMA stimulation, the muscle type acylphosphatase mRNA levels remain constant also after the treatment with lipopolysaccharide and γ-interferon, treatments that lead to macrophage activation. It is possible that post-transcription regulation is responsible for the regulation of muscle type acylphosphatase in the cell during differentiation and macrophage activation.</description><subject>Acid Anhydride Hydrolases - genetics</subject><subject>Acid Anhydride Hydrolases - metabolism</subject><subject>Acylphosphatase</subject><subject>Base Sequence</subject><subject>Cell Differentiation - drug effects</subject><subject>differentiation</subject><subject>DNA Primers - genetics</subject><subject>Gene Expression</subject><subject>Humans</subject><subject>Isoenzymes - genetics</subject><subject>Isoenzymes - metabolism</subject><subject>Macrophage Activation - physiology</subject><subject>Macrophages - cytology</subject><subject>Macrophages - drug effects</subject><subject>Macrophages - enzymology</subject><subject>Nitric Oxide - biosynthesis</subject><subject>post-transcription regulation</subject><subject>RNA, Messenger - genetics</subject><subject>RNA, Messenger - metabolism</subject><subject>Tetradecanoylphorbol Acetate - pharmacology</subject><subject>U-937</subject><subject>U937 Cells</subject><issn>0300-9084</issn><issn>1638-6183</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1999</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkEtLxDAQgIMouj5-gtKT6KE6aZomPckivkDwoF4NaTLZjXTbmnQX99_btSLePA3DfPP6CDmmcEGBFpfPwADSEmR-VpbnAIyxVG6RCS2YTAsq2TaZ_CJ7ZD_GdwDgkJW7ZI8CF5xxOSFvU7Ouu3kbu7nudcQEP7uAMfq2Sewy-GaWLLQJ7VCeYWK9cxiw6b3uN4RubKJN71dj2rrkNS2ZSAzWdVL7Bg_JjtN1xKOfeEBeb29eru_Tx6e7h-vpY2ryPO_TSmiNlAmkleMOZCWFyKgQjtvMWgeGC1tUglOswFiUmXRGZsC4kAXHnLIDcjrO7UL7scTYq4WPmyt0g-0yqgzykgLLBpCP4PBTjAGd6oJf6LBWFNRGrPoWqzbWVFmqb7FKDn0nPwuW1QLtn67R5ABcjQAOb648BhWNx8ag9QFNr2zr_1nxBduYiNo</recordid><startdate>19991101</startdate><enddate>19991101</enddate><creator>Degl'Innocenti, Donatella</creator><creator>Marzocchini, Riccardo</creator><creator>Rosati, Fabiana</creator><creator>Cellini, Elena</creator><creator>Raugei, Giovanni</creator><creator>Ramponi, Giampietro</creator><general>Elsevier Masson SAS</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7T5</scope><scope>H94</scope></search><sort><creationdate>19991101</creationdate><title>Acylphosphatase expression during macrophage differentiation and activation of U-937 cell line</title><author>Degl'Innocenti, Donatella ; Marzocchini, Riccardo ; Rosati, Fabiana ; Cellini, Elena ; Raugei, Giovanni ; Ramponi, Giampietro</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c444t-b7aae137e1bf5f08b8772177f5d2ddf0c57d6b751eb0cde828fc820357865e413</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1999</creationdate><topic>Acid Anhydride Hydrolases - genetics</topic><topic>Acid Anhydride Hydrolases - metabolism</topic><topic>Acylphosphatase</topic><topic>Base Sequence</topic><topic>Cell Differentiation - drug effects</topic><topic>differentiation</topic><topic>DNA Primers - genetics</topic><topic>Gene Expression</topic><topic>Humans</topic><topic>Isoenzymes - genetics</topic><topic>Isoenzymes - metabolism</topic><topic>Macrophage Activation - physiology</topic><topic>Macrophages - cytology</topic><topic>Macrophages - drug effects</topic><topic>Macrophages - enzymology</topic><topic>Nitric Oxide - biosynthesis</topic><topic>post-transcription regulation</topic><topic>RNA, Messenger - genetics</topic><topic>RNA, Messenger - metabolism</topic><topic>Tetradecanoylphorbol Acetate - pharmacology</topic><topic>U-937</topic><topic>U937 Cells</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Degl'Innocenti, Donatella</creatorcontrib><creatorcontrib>Marzocchini, Riccardo</creatorcontrib><creatorcontrib>Rosati, Fabiana</creatorcontrib><creatorcontrib>Cellini, Elena</creatorcontrib><creatorcontrib>Raugei, Giovanni</creatorcontrib><creatorcontrib>Ramponi, Giampietro</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Immunology Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><jtitle>Biochimie</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Degl'Innocenti, Donatella</au><au>Marzocchini, Riccardo</au><au>Rosati, Fabiana</au><au>Cellini, Elena</au><au>Raugei, Giovanni</au><au>Ramponi, Giampietro</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Acylphosphatase expression during macrophage differentiation and activation of U-937 cell line</atitle><jtitle>Biochimie</jtitle><addtitle>Biochimie</addtitle><date>1999-11-01</date><risdate>1999</risdate><volume>81</volume><issue>11</issue><spage>1031</spage><epage>1035</epage><pages>1031-1035</pages><issn>0300-9084</issn><eissn>1638-6183</eissn><abstract>The two acylphosphatase isoenzymes (muscle type and common type) are differently involved in cell differentiation processes. In this paper we investigate the expression of the two isoenzymes during macrophage differentiation and activation. The U-937 human promonocytic cell line is a model for cell differentiation induced by the tumor promoter phorbol myristic acetate (PMA). Here we show that only the expression of the muscle type acylphosphatase increases during U-937 differentiation and macrophage activation, confirming that the two isoenzymes are differently regulated. Moreover, we determined, in the same conditions, the level of specific mRNA. Results show that after an initial two-fold decrease during PMA stimulation, the muscle type acylphosphatase mRNA levels remain constant also after the treatment with lipopolysaccharide and γ-interferon, treatments that lead to macrophage activation. 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| subjects | Acid Anhydride Hydrolases - genetics Acid Anhydride Hydrolases - metabolism Acylphosphatase Base Sequence Cell Differentiation - drug effects differentiation DNA Primers - genetics Gene Expression Humans Isoenzymes - genetics Isoenzymes - metabolism Macrophage Activation - physiology Macrophages - cytology Macrophages - drug effects Macrophages - enzymology Nitric Oxide - biosynthesis post-transcription regulation RNA, Messenger - genetics RNA, Messenger - metabolism Tetradecanoylphorbol Acetate - pharmacology U-937 U937 Cells |
| title | Acylphosphatase expression during macrophage differentiation and activation of U-937 cell line |
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