Responses to 5-HT in morphologically identified neurons in the rat substantia gelatinosa in vitro
Abstract Bath application of 5-HT (1-1000 μM) induced a tetrodotoxin (TTX)-resistant outward current at the holding membrane potential (VH ) of −50 mV in 104/162 (64.2%) of substantia gelatinosa (SG) neurons from the rat spinal cord in vitro . The 5-HT-induced outward current was suppressed by an ex...
Gespeichert in:
| Veröffentlicht in: | Neuroscience 2009-03, Vol.159 (1), p.316-324 |
|---|---|
| Hauptverfasser: | , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 324 |
|---|---|
| container_issue | 1 |
| container_start_page | 316 |
| container_title | Neuroscience |
| container_volume | 159 |
| creator | Abe, K Kato, G Katafuchi, T Tamae, A Furue, H Yoshimura, M |
| description | Abstract Bath application of 5-HT (1-1000 μM) induced a tetrodotoxin (TTX)-resistant outward current at the holding membrane potential (VH ) of −50 mV in 104/162 (64.2%) of substantia gelatinosa (SG) neurons from the rat spinal cord in vitro . The 5-HT-induced outward current was suppressed by an external solution containing Ba2+ , or a pipette solution containing Cs2 SO4 and tetraethylammonium. It was reversed near the equilibrium potential of the K+ channel. The response to 5-HT was abolished 30 min after patch formation with a pipette solution containing guanosine-5-O-(2-thiodiphosphate)-S. The 5-HT-induced outward current was mimicked by a 5-HT1A agonist, (±)-8-hydroxy-2-(di-n-propylamino) tetralin hydrobromide, and suppressed by a 5-HT1A antagonist, WAY100635, suggesting the 5HT1A receptor-mediated activation of K+ channels in the outward current. In 11/162 (6.8%) SG neurons, 5-HT produced an inward current, which was mimicked by a 5-HT3 agonist, 1-( m -chlorophenyl)-biguanide (mCPBG). The 5-HT-induced outward currents were observed in vertical cells (21/34) and small islet cells (11/34), while inward currents were induced in islet cells (1/5) and small islet (4/5) cells, but not in vertical cells. It is known that most vertical cells and islet cells in the SG are excitatory (glutamatergic) and inhibitory interneurons, respectively, while small islet cells consist of both excitatory and inhibitory neurons. Bath application of 5-HT or mCPBG increased the amplitude and the frequency of spontaneous inhibitory postsynaptic currents (sIPSCs), but no neurons showed a decrease in sIPSC. Furthermore, frequency, but not amplitude, of miniature IPSCs increased with perfusion with 5-HT in the presence of TTX. These findings, taken together, suggest that 5-HT induces outward currents through 5-HT1A receptors in excitatory SG neurons. These findings also suggest that the inward currents are post- and presynaptically evoked through 5-HT3 receptors, probably in inhibitory neurons. |
| doi_str_mv | 10.1016/j.neuroscience.2008.12.021 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_20480116</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>S0306452208017983</els_id><sourcerecordid>20480116</sourcerecordid><originalsourceid>FETCH-LOGICAL-c560t-f58403c981037c6c262b6625fc8f3a6b5a33b6f6604b55ed48eb9e7569a763</originalsourceid><addsrcrecordid>eNqNks1u1DAURi0EokPhFVCEBLsEXzt2EhZIVfkpUiWktnvLcW5aDxl7sJ1K8_Z1OhEgVvXGm_Pdzzq-hLwDWgEF-XFbOZyDj8aiM1gxStsKWEUZPCMbaBteNqKun5MN5VSWtWDshLyKcUvzETV_SU6ggxo48A3RVxj33kWMRfKFKC9uCuuKnQ_7Oz_5W2v0NB0KO6BLdrQ4FI_VLi5UusMi6FTEuY9JZ0AXtzjpZJ2PegHubQr-NXkx6inim_U-JVffvt6cX5SXP7__OD-7LI2QNJWjaGvKTdcC5Y2RhknWS8nEaNqRa9kLzXkvRylp3QuBQ91i32EjZKcbyU_Jh-PQffC_Z4xJ7Ww0OE3aoZ-jYrRuKcACfjqCJguMAUe1D3anw0EBVYtdtVX_2lWLXQVMZbs5_HZtmfsdDn-jq84MvF8BHbO5MWhnbPzDMWDQglwGfTlymIXcWwxqrRtsQJPU4O3T3vP5vzFmsm75s194wLj1c3BZuQIVc0BdL_uwrAPNLpqu5fwB9tO0tg</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>20480116</pqid></control><display><type>article</type><title>Responses to 5-HT in morphologically identified neurons in the rat substantia gelatinosa in vitro</title><source>MEDLINE</source><source>Access via ScienceDirect (Elsevier)</source><creator>Abe, K ; Kato, G ; Katafuchi, T ; Tamae, A ; Furue, H ; Yoshimura, M</creator><creatorcontrib>Abe, K ; Kato, G ; Katafuchi, T ; Tamae, A ; Furue, H ; Yoshimura, M</creatorcontrib><description>Abstract Bath application of 5-HT (1-1000 μM) induced a tetrodotoxin (TTX)-resistant outward current at the holding membrane potential (VH ) of −50 mV in 104/162 (64.2%) of substantia gelatinosa (SG) neurons from the rat spinal cord in vitro . The 5-HT-induced outward current was suppressed by an external solution containing Ba2+ , or a pipette solution containing Cs2 SO4 and tetraethylammonium. It was reversed near the equilibrium potential of the K+ channel. The response to 5-HT was abolished 30 min after patch formation with a pipette solution containing guanosine-5-O-(2-thiodiphosphate)-S. The 5-HT-induced outward current was mimicked by a 5-HT1A agonist, (±)-8-hydroxy-2-(di-n-propylamino) tetralin hydrobromide, and suppressed by a 5-HT1A antagonist, WAY100635, suggesting the 5HT1A receptor-mediated activation of K+ channels in the outward current. In 11/162 (6.8%) SG neurons, 5-HT produced an inward current, which was mimicked by a 5-HT3 agonist, 1-( m -chlorophenyl)-biguanide (mCPBG). The 5-HT-induced outward currents were observed in vertical cells (21/34) and small islet cells (11/34), while inward currents were induced in islet cells (1/5) and small islet (4/5) cells, but not in vertical cells. It is known that most vertical cells and islet cells in the SG are excitatory (glutamatergic) and inhibitory interneurons, respectively, while small islet cells consist of both excitatory and inhibitory neurons. Bath application of 5-HT or mCPBG increased the amplitude and the frequency of spontaneous inhibitory postsynaptic currents (sIPSCs), but no neurons showed a decrease in sIPSC. Furthermore, frequency, but not amplitude, of miniature IPSCs increased with perfusion with 5-HT in the presence of TTX. These findings, taken together, suggest that 5-HT induces outward currents through 5-HT1A receptors in excitatory SG neurons. These findings also suggest that the inward currents are post- and presynaptically evoked through 5-HT3 receptors, probably in inhibitory neurons.</description><identifier>ISSN: 0306-4522</identifier><identifier>EISSN: 1873-7544</identifier><identifier>DOI: 10.1016/j.neuroscience.2008.12.021</identifier><identifier>PMID: 19141313</identifier><identifier>CODEN: NRSCDN</identifier><language>eng</language><publisher>Amsterdam: Elsevier Ltd</publisher><subject>5-HT ; Animals ; Biological and medical sciences ; Biophysical Phenomena - drug effects ; Biophysics ; Biotin - analogs & derivatives ; Biotin - metabolism ; Cell Size - drug effects ; Dose-Response Relationship, Drug ; Electric Stimulation - methods ; Fundamental and applied biological sciences. Psychology ; Guanosine 5'-O-(3-Thiotriphosphate) - analogs & derivatives ; Guanosine 5'-O-(3-Thiotriphosphate) - pharmacology ; In Vitro Techniques ; inhibitory postsynaptic current ; Inhibitory Postsynaptic Potentials - drug effects ; IPSC ; Membrane Potentials - drug effects ; Neural Inhibition - drug effects ; Neurology ; Neurons - cytology ; Neurons - drug effects ; outward current ; patch-clamp ; Patch-Clamp Techniques - methods ; Potassium Channel Blockers - pharmacology ; Rats ; Serotonin - pharmacology ; Serotonin Antagonists - pharmacology ; Serotonin Receptor Agonists - pharmacology ; Sodium Channel Blockers - pharmacology ; Spinal Cord - anatomy & histology ; substantia gelatinosa ; Substantia Gelatinosa - cytology ; Tetraethylammonium - pharmacology ; Tetrodotoxin - pharmacology ; Vertebrates: nervous system and sense organs</subject><ispartof>Neuroscience, 2009-03, Vol.159 (1), p.316-324</ispartof><rights>IBRO</rights><rights>2009 IBRO</rights><rights>2009 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c560t-f58403c981037c6c262b6625fc8f3a6b5a33b6f6604b55ed48eb9e7569a763</citedby><cites>FETCH-LOGICAL-c560t-f58403c981037c6c262b6625fc8f3a6b5a33b6f6604b55ed48eb9e7569a763</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.neuroscience.2008.12.021$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=21218161$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/19141313$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Abe, K</creatorcontrib><creatorcontrib>Kato, G</creatorcontrib><creatorcontrib>Katafuchi, T</creatorcontrib><creatorcontrib>Tamae, A</creatorcontrib><creatorcontrib>Furue, H</creatorcontrib><creatorcontrib>Yoshimura, M</creatorcontrib><title>Responses to 5-HT in morphologically identified neurons in the rat substantia gelatinosa in vitro</title><title>Neuroscience</title><addtitle>Neuroscience</addtitle><description>Abstract Bath application of 5-HT (1-1000 μM) induced a tetrodotoxin (TTX)-resistant outward current at the holding membrane potential (VH ) of −50 mV in 104/162 (64.2%) of substantia gelatinosa (SG) neurons from the rat spinal cord in vitro . The 5-HT-induced outward current was suppressed by an external solution containing Ba2+ , or a pipette solution containing Cs2 SO4 and tetraethylammonium. It was reversed near the equilibrium potential of the K+ channel. The response to 5-HT was abolished 30 min after patch formation with a pipette solution containing guanosine-5-O-(2-thiodiphosphate)-S. The 5-HT-induced outward current was mimicked by a 5-HT1A agonist, (±)-8-hydroxy-2-(di-n-propylamino) tetralin hydrobromide, and suppressed by a 5-HT1A antagonist, WAY100635, suggesting the 5HT1A receptor-mediated activation of K+ channels in the outward current. In 11/162 (6.8%) SG neurons, 5-HT produced an inward current, which was mimicked by a 5-HT3 agonist, 1-( m -chlorophenyl)-biguanide (mCPBG). The 5-HT-induced outward currents were observed in vertical cells (21/34) and small islet cells (11/34), while inward currents were induced in islet cells (1/5) and small islet (4/5) cells, but not in vertical cells. It is known that most vertical cells and islet cells in the SG are excitatory (glutamatergic) and inhibitory interneurons, respectively, while small islet cells consist of both excitatory and inhibitory neurons. Bath application of 5-HT or mCPBG increased the amplitude and the frequency of spontaneous inhibitory postsynaptic currents (sIPSCs), but no neurons showed a decrease in sIPSC. Furthermore, frequency, but not amplitude, of miniature IPSCs increased with perfusion with 5-HT in the presence of TTX. These findings, taken together, suggest that 5-HT induces outward currents through 5-HT1A receptors in excitatory SG neurons. These findings also suggest that the inward currents are post- and presynaptically evoked through 5-HT3 receptors, probably in inhibitory neurons.</description><subject>5-HT</subject><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Biophysical Phenomena - drug effects</subject><subject>Biophysics</subject><subject>Biotin - analogs & derivatives</subject><subject>Biotin - metabolism</subject><subject>Cell Size - drug effects</subject><subject>Dose-Response Relationship, Drug</subject><subject>Electric Stimulation - methods</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Guanosine 5'-O-(3-Thiotriphosphate) - analogs & derivatives</subject><subject>Guanosine 5'-O-(3-Thiotriphosphate) - pharmacology</subject><subject>In Vitro Techniques</subject><subject>inhibitory postsynaptic current</subject><subject>Inhibitory Postsynaptic Potentials - drug effects</subject><subject>IPSC</subject><subject>Membrane Potentials - drug effects</subject><subject>Neural Inhibition - drug effects</subject><subject>Neurology</subject><subject>Neurons - cytology</subject><subject>Neurons - drug effects</subject><subject>outward current</subject><subject>patch-clamp</subject><subject>Patch-Clamp Techniques - methods</subject><subject>Potassium Channel Blockers - pharmacology</subject><subject>Rats</subject><subject>Serotonin - pharmacology</subject><subject>Serotonin Antagonists - pharmacology</subject><subject>Serotonin Receptor Agonists - pharmacology</subject><subject>Sodium Channel Blockers - pharmacology</subject><subject>Spinal Cord - anatomy & histology</subject><subject>substantia gelatinosa</subject><subject>Substantia Gelatinosa - cytology</subject><subject>Tetraethylammonium - pharmacology</subject><subject>Tetrodotoxin - pharmacology</subject><subject>Vertebrates: nervous system and sense organs</subject><issn>0306-4522</issn><issn>1873-7544</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2009</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNks1u1DAURi0EokPhFVCEBLsEXzt2EhZIVfkpUiWktnvLcW5aDxl7sJ1K8_Z1OhEgVvXGm_Pdzzq-hLwDWgEF-XFbOZyDj8aiM1gxStsKWEUZPCMbaBteNqKun5MN5VSWtWDshLyKcUvzETV_SU6ggxo48A3RVxj33kWMRfKFKC9uCuuKnQ_7Oz_5W2v0NB0KO6BLdrQ4FI_VLi5UusMi6FTEuY9JZ0AXtzjpZJ2PegHubQr-NXkx6inim_U-JVffvt6cX5SXP7__OD-7LI2QNJWjaGvKTdcC5Y2RhknWS8nEaNqRa9kLzXkvRylp3QuBQ91i32EjZKcbyU_Jh-PQffC_Z4xJ7Ww0OE3aoZ-jYrRuKcACfjqCJguMAUe1D3anw0EBVYtdtVX_2lWLXQVMZbs5_HZtmfsdDn-jq84MvF8BHbO5MWhnbPzDMWDQglwGfTlymIXcWwxqrRtsQJPU4O3T3vP5vzFmsm75s194wLj1c3BZuQIVc0BdL_uwrAPNLpqu5fwB9tO0tg</recordid><startdate>20090303</startdate><enddate>20090303</enddate><creator>Abe, K</creator><creator>Kato, G</creator><creator>Katafuchi, T</creator><creator>Tamae, A</creator><creator>Furue, H</creator><creator>Yoshimura, M</creator><general>Elsevier Ltd</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TK</scope></search><sort><creationdate>20090303</creationdate><title>Responses to 5-HT in morphologically identified neurons in the rat substantia gelatinosa in vitro</title><author>Abe, K ; Kato, G ; Katafuchi, T ; Tamae, A ; Furue, H ; Yoshimura, M</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c560t-f58403c981037c6c262b6625fc8f3a6b5a33b6f6604b55ed48eb9e7569a763</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2009</creationdate><topic>5-HT</topic><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Biophysical Phenomena - drug effects</topic><topic>Biophysics</topic><topic>Biotin - analogs & derivatives</topic><topic>Biotin - metabolism</topic><topic>Cell Size - drug effects</topic><topic>Dose-Response Relationship, Drug</topic><topic>Electric Stimulation - methods</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Guanosine 5'-O-(3-Thiotriphosphate) - analogs & derivatives</topic><topic>Guanosine 5'-O-(3-Thiotriphosphate) - pharmacology</topic><topic>In Vitro Techniques</topic><topic>inhibitory postsynaptic current</topic><topic>Inhibitory Postsynaptic Potentials - drug effects</topic><topic>IPSC</topic><topic>Membrane Potentials - drug effects</topic><topic>Neural Inhibition - drug effects</topic><topic>Neurology</topic><topic>Neurons - cytology</topic><topic>Neurons - drug effects</topic><topic>outward current</topic><topic>patch-clamp</topic><topic>Patch-Clamp Techniques - methods</topic><topic>Potassium Channel Blockers - pharmacology</topic><topic>Rats</topic><topic>Serotonin - pharmacology</topic><topic>Serotonin Antagonists - pharmacology</topic><topic>Serotonin Receptor Agonists - pharmacology</topic><topic>Sodium Channel Blockers - pharmacology</topic><topic>Spinal Cord - anatomy & histology</topic><topic>substantia gelatinosa</topic><topic>Substantia Gelatinosa - cytology</topic><topic>Tetraethylammonium - pharmacology</topic><topic>Tetrodotoxin - pharmacology</topic><topic>Vertebrates: nervous system and sense organs</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Abe, K</creatorcontrib><creatorcontrib>Kato, G</creatorcontrib><creatorcontrib>Katafuchi, T</creatorcontrib><creatorcontrib>Tamae, A</creatorcontrib><creatorcontrib>Furue, H</creatorcontrib><creatorcontrib>Yoshimura, M</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Neurosciences Abstracts</collection><jtitle>Neuroscience</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Abe, K</au><au>Kato, G</au><au>Katafuchi, T</au><au>Tamae, A</au><au>Furue, H</au><au>Yoshimura, M</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Responses to 5-HT in morphologically identified neurons in the rat substantia gelatinosa in vitro</atitle><jtitle>Neuroscience</jtitle><addtitle>Neuroscience</addtitle><date>2009-03-03</date><risdate>2009</risdate><volume>159</volume><issue>1</issue><spage>316</spage><epage>324</epage><pages>316-324</pages><issn>0306-4522</issn><eissn>1873-7544</eissn><coden>NRSCDN</coden><abstract>Abstract Bath application of 5-HT (1-1000 μM) induced a tetrodotoxin (TTX)-resistant outward current at the holding membrane potential (VH ) of −50 mV in 104/162 (64.2%) of substantia gelatinosa (SG) neurons from the rat spinal cord in vitro . The 5-HT-induced outward current was suppressed by an external solution containing Ba2+ , or a pipette solution containing Cs2 SO4 and tetraethylammonium. It was reversed near the equilibrium potential of the K+ channel. The response to 5-HT was abolished 30 min after patch formation with a pipette solution containing guanosine-5-O-(2-thiodiphosphate)-S. The 5-HT-induced outward current was mimicked by a 5-HT1A agonist, (±)-8-hydroxy-2-(di-n-propylamino) tetralin hydrobromide, and suppressed by a 5-HT1A antagonist, WAY100635, suggesting the 5HT1A receptor-mediated activation of K+ channels in the outward current. In 11/162 (6.8%) SG neurons, 5-HT produced an inward current, which was mimicked by a 5-HT3 agonist, 1-( m -chlorophenyl)-biguanide (mCPBG). The 5-HT-induced outward currents were observed in vertical cells (21/34) and small islet cells (11/34), while inward currents were induced in islet cells (1/5) and small islet (4/5) cells, but not in vertical cells. It is known that most vertical cells and islet cells in the SG are excitatory (glutamatergic) and inhibitory interneurons, respectively, while small islet cells consist of both excitatory and inhibitory neurons. Bath application of 5-HT or mCPBG increased the amplitude and the frequency of spontaneous inhibitory postsynaptic currents (sIPSCs), but no neurons showed a decrease in sIPSC. Furthermore, frequency, but not amplitude, of miniature IPSCs increased with perfusion with 5-HT in the presence of TTX. These findings, taken together, suggest that 5-HT induces outward currents through 5-HT1A receptors in excitatory SG neurons. These findings also suggest that the inward currents are post- and presynaptically evoked through 5-HT3 receptors, probably in inhibitory neurons.</abstract><cop>Amsterdam</cop><pub>Elsevier Ltd</pub><pmid>19141313</pmid><doi>10.1016/j.neuroscience.2008.12.021</doi><tpages>9</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0306-4522 |
| ispartof | Neuroscience, 2009-03, Vol.159 (1), p.316-324 |
| issn | 0306-4522 1873-7544 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_20480116 |
| source | MEDLINE; Access via ScienceDirect (Elsevier) |
| subjects | 5-HT Animals Biological and medical sciences Biophysical Phenomena - drug effects Biophysics Biotin - analogs & derivatives Biotin - metabolism Cell Size - drug effects Dose-Response Relationship, Drug Electric Stimulation - methods Fundamental and applied biological sciences. Psychology Guanosine 5'-O-(3-Thiotriphosphate) - analogs & derivatives Guanosine 5'-O-(3-Thiotriphosphate) - pharmacology In Vitro Techniques inhibitory postsynaptic current Inhibitory Postsynaptic Potentials - drug effects IPSC Membrane Potentials - drug effects Neural Inhibition - drug effects Neurology Neurons - cytology Neurons - drug effects outward current patch-clamp Patch-Clamp Techniques - methods Potassium Channel Blockers - pharmacology Rats Serotonin - pharmacology Serotonin Antagonists - pharmacology Serotonin Receptor Agonists - pharmacology Sodium Channel Blockers - pharmacology Spinal Cord - anatomy & histology substantia gelatinosa Substantia Gelatinosa - cytology Tetraethylammonium - pharmacology Tetrodotoxin - pharmacology Vertebrates: nervous system and sense organs |
| title | Responses to 5-HT in morphologically identified neurons in the rat substantia gelatinosa in vitro |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-19T10%3A46%3A38IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Responses%20to%205-HT%20in%20morphologically%20identified%20neurons%20in%20the%20rat%20substantia%20gelatinosa%20in%20vitro&rft.jtitle=Neuroscience&rft.au=Abe,%20K&rft.date=2009-03-03&rft.volume=159&rft.issue=1&rft.spage=316&rft.epage=324&rft.pages=316-324&rft.issn=0306-4522&rft.eissn=1873-7544&rft.coden=NRSCDN&rft_id=info:doi/10.1016/j.neuroscience.2008.12.021&rft_dat=%3Cproquest_cross%3E20480116%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=20480116&rft_id=info:pmid/19141313&rft_els_id=S0306452208017983&rfr_iscdi=true |