Expression of the Arabidopsis metallothionein 2b enhances arsenite sensitivity and root to shoot translocation in tobacco
We expressed the AtMT2b gene under the 35 S cauliflower mosaic virus promoter in Nicotiana tabacum (Sr1), using leaf disc transformation. Arsenite tolerance and uptake, as well as arsenite-induced phytochelatin (PC) accumulation in roots were measured in transgenic lines, and compared to untransform...
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| Veröffentlicht in: | Environmental and experimental botany 2009-04, Vol.66 (1), p.69-73 |
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| creator | Grispen, Veerle M.J. Irtelli, Barbara Hakvoort, Henk W.J. Vooijs, Riet Bliek, Tijs ten Bookum, Wilma M. Verkleij, Jos A.C. Schat, Henk |
| description | We expressed the
AtMT2b gene under the 35 S cauliflower mosaic virus promoter in
Nicotiana tabacum (Sr1), using leaf disc transformation. Arsenite tolerance and uptake, as well as arsenite-induced phytochelatin (PC) accumulation in roots were measured in transgenic lines, and compared to untransformed (‘wild type’) tobacco. Measured after 5 days of exposure, arsenite tolerance was slightly but significantly decreased in the transgenic lines compared to wild type. The highest
AtMT2b expressing line exhibited a significantly decreased arsenic accumulation in roots, but an increased accumulation in shoots, while the total amount of arsenic taken up remained unchanged, suggesting that
AtMT2b expression enhanced the arsenic root to shoot transport. The same transformant line also exhibited a decreased rate of phytochelatin accumulation in the roots, but the phytochelatin-SH to As molar ratio was higher than in wild type, suggesting that the lower arsenite tolerance in the transformant lines was not due to a potential shortage of cysteine for PC synthesis, imposed by expression of the transgene. |
| doi_str_mv | 10.1016/j.envexpbot.2008.12.021 |
| format | Article |
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AtMT2b gene under the 35 S cauliflower mosaic virus promoter in
Nicotiana tabacum (Sr1), using leaf disc transformation. Arsenite tolerance and uptake, as well as arsenite-induced phytochelatin (PC) accumulation in roots were measured in transgenic lines, and compared to untransformed (‘wild type’) tobacco. Measured after 5 days of exposure, arsenite tolerance was slightly but significantly decreased in the transgenic lines compared to wild type. The highest
AtMT2b expressing line exhibited a significantly decreased arsenic accumulation in roots, but an increased accumulation in shoots, while the total amount of arsenic taken up remained unchanged, suggesting that
AtMT2b expression enhanced the arsenic root to shoot transport. The same transformant line also exhibited a decreased rate of phytochelatin accumulation in the roots, but the phytochelatin-SH to As molar ratio was higher than in wild type, suggesting that the lower arsenite tolerance in the transformant lines was not due to a potential shortage of cysteine for PC synthesis, imposed by expression of the transgene.</description><identifier>ISSN: 0098-8472</identifier><identifier>EISSN: 1873-7307</identifier><identifier>DOI: 10.1016/j.envexpbot.2008.12.021</identifier><identifier>CODEN: EEBODM</identifier><language>eng</language><publisher>Amsterdam: Elsevier B.V</publisher><subject>Arabidopsis ; Arabidopsis thaliana ; Arabidopsis thaliana metallothionein 2b ; Arsenite toxicity ; arsenites ; Biological and medical sciences ; Cauliflower mosaic virus ; chelating agents ; cysteine ; Fundamental and applied biological sciences. Psychology ; genes ; genetic transformation ; metal tolerance ; metallothionein ; Nicotiana tabacum ; Nicotiana tabacum SR1 ; Phytochelatins ; roots ; shoots ; tobacco ; transgenic plants ; translocation (plant physiology) ; uptake mechanisms</subject><ispartof>Environmental and experimental botany, 2009-04, Vol.66 (1), p.69-73</ispartof><rights>2009 Elsevier B.V.</rights><rights>2009 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c431t-a86c7e236929acfbb5bb1b979eb24693df814f83b6d0ed74c1f9371bffb0aed63</citedby><cites>FETCH-LOGICAL-c431t-a86c7e236929acfbb5bb1b979eb24693df814f83b6d0ed74c1f9371bffb0aed63</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.envexpbot.2008.12.021$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,777,781,3537,27905,27906,45976</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=21300487$$DView record in Pascal Francis$$Hfree_for_read</backlink></links><search><creatorcontrib>Grispen, Veerle M.J.</creatorcontrib><creatorcontrib>Irtelli, Barbara</creatorcontrib><creatorcontrib>Hakvoort, Henk W.J.</creatorcontrib><creatorcontrib>Vooijs, Riet</creatorcontrib><creatorcontrib>Bliek, Tijs</creatorcontrib><creatorcontrib>ten Bookum, Wilma M.</creatorcontrib><creatorcontrib>Verkleij, Jos A.C.</creatorcontrib><creatorcontrib>Schat, Henk</creatorcontrib><title>Expression of the Arabidopsis metallothionein 2b enhances arsenite sensitivity and root to shoot translocation in tobacco</title><title>Environmental and experimental botany</title><description>We expressed the
AtMT2b gene under the 35 S cauliflower mosaic virus promoter in
Nicotiana tabacum (Sr1), using leaf disc transformation. Arsenite tolerance and uptake, as well as arsenite-induced phytochelatin (PC) accumulation in roots were measured in transgenic lines, and compared to untransformed (‘wild type’) tobacco. Measured after 5 days of exposure, arsenite tolerance was slightly but significantly decreased in the transgenic lines compared to wild type. The highest
AtMT2b expressing line exhibited a significantly decreased arsenic accumulation in roots, but an increased accumulation in shoots, while the total amount of arsenic taken up remained unchanged, suggesting that
AtMT2b expression enhanced the arsenic root to shoot transport. The same transformant line also exhibited a decreased rate of phytochelatin accumulation in the roots, but the phytochelatin-SH to As molar ratio was higher than in wild type, suggesting that the lower arsenite tolerance in the transformant lines was not due to a potential shortage of cysteine for PC synthesis, imposed by expression of the transgene.</description><subject>Arabidopsis</subject><subject>Arabidopsis thaliana</subject><subject>Arabidopsis thaliana metallothionein 2b</subject><subject>Arsenite toxicity</subject><subject>arsenites</subject><subject>Biological and medical sciences</subject><subject>Cauliflower mosaic virus</subject><subject>chelating agents</subject><subject>cysteine</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>genes</subject><subject>genetic transformation</subject><subject>metal tolerance</subject><subject>metallothionein</subject><subject>Nicotiana tabacum</subject><subject>Nicotiana tabacum SR1</subject><subject>Phytochelatins</subject><subject>roots</subject><subject>shoots</subject><subject>tobacco</subject><subject>transgenic plants</subject><subject>translocation (plant physiology)</subject><subject>uptake mechanisms</subject><issn>0098-8472</issn><issn>1873-7307</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2009</creationdate><recordtype>article</recordtype><recordid>eNqFkU9r3DAQxUVpodttP0N0aW929S-WfVxCmhYCPbQ5C0kedbV4LVejLNlvX7kbcg0IRjC_eW-YR8gVZy1nvPt6aGE-wdPiUmkFY33LRcsEf0M2vNey0ZLpt2TD2NA3vdLiPfmAeGCMaam7DTnfPi0ZEGOaaQq07IHusnVxTAtGpEcodppS2dc-xJkKR2He29kDUpsR5liA1oKxxFMsZ2rnkeaUCi2J4v7_J9sZp-RtWT2qRknOep8-knfBTgifnuuWPHy7_X3zvbn_effjZnffeCV5aWzfeQ1CdoMYrA_OXTvH3aAHcEJ1gxxDz1XopetGBqNWnodBau5CcMzC2Mkt-XLRXXL6-whYzDGih2myM6RHNIIprdUgXwW5YkLL-rZEX0CfE2KGYJYcjzafDWdmzcQczEsmZs3EcGFqJnXy87OFRW-nUE_jI76MV4Ix1a8OVxcu2GTsn1yZh1-C1Tbv2LVQq9LuQkA93SlCNugj1FzGmMEXM6b46jb_AHDis6Y</recordid><startdate>20090401</startdate><enddate>20090401</enddate><creator>Grispen, Veerle M.J.</creator><creator>Irtelli, Barbara</creator><creator>Hakvoort, Henk W.J.</creator><creator>Vooijs, Riet</creator><creator>Bliek, Tijs</creator><creator>ten Bookum, Wilma M.</creator><creator>Verkleij, Jos A.C.</creator><creator>Schat, Henk</creator><general>Elsevier B.V</general><general>Oxford; New York, NY: Elsevier Science</general><general>Elsevier</general><scope>FBQ</scope><scope>IQODW</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7ST</scope><scope>C1K</scope><scope>SOI</scope><scope>7TV</scope><scope>7U6</scope><scope>7U9</scope><scope>H94</scope></search><sort><creationdate>20090401</creationdate><title>Expression of the Arabidopsis metallothionein 2b enhances arsenite sensitivity and root to shoot translocation in tobacco</title><author>Grispen, Veerle M.J. ; Irtelli, Barbara ; Hakvoort, Henk W.J. ; Vooijs, Riet ; Bliek, Tijs ; ten Bookum, Wilma M. ; Verkleij, Jos A.C. ; Schat, Henk</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c431t-a86c7e236929acfbb5bb1b979eb24693df814f83b6d0ed74c1f9371bffb0aed63</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2009</creationdate><topic>Arabidopsis</topic><topic>Arabidopsis thaliana</topic><topic>Arabidopsis thaliana metallothionein 2b</topic><topic>Arsenite toxicity</topic><topic>arsenites</topic><topic>Biological and medical sciences</topic><topic>Cauliflower mosaic virus</topic><topic>chelating agents</topic><topic>cysteine</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>genes</topic><topic>genetic transformation</topic><topic>metal tolerance</topic><topic>metallothionein</topic><topic>Nicotiana tabacum</topic><topic>Nicotiana tabacum SR1</topic><topic>Phytochelatins</topic><topic>roots</topic><topic>shoots</topic><topic>tobacco</topic><topic>transgenic plants</topic><topic>translocation (plant physiology)</topic><topic>uptake mechanisms</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Grispen, Veerle M.J.</creatorcontrib><creatorcontrib>Irtelli, Barbara</creatorcontrib><creatorcontrib>Hakvoort, Henk W.J.</creatorcontrib><creatorcontrib>Vooijs, Riet</creatorcontrib><creatorcontrib>Bliek, Tijs</creatorcontrib><creatorcontrib>ten Bookum, Wilma M.</creatorcontrib><creatorcontrib>Verkleij, Jos A.C.</creatorcontrib><creatorcontrib>Schat, Henk</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>CrossRef</collection><collection>Environment Abstracts</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Environment Abstracts</collection><collection>Pollution Abstracts</collection><collection>Sustainability Science Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><jtitle>Environmental and experimental botany</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Grispen, Veerle M.J.</au><au>Irtelli, Barbara</au><au>Hakvoort, Henk W.J.</au><au>Vooijs, Riet</au><au>Bliek, Tijs</au><au>ten Bookum, Wilma M.</au><au>Verkleij, Jos A.C.</au><au>Schat, Henk</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Expression of the Arabidopsis metallothionein 2b enhances arsenite sensitivity and root to shoot translocation in tobacco</atitle><jtitle>Environmental and experimental botany</jtitle><date>2009-04-01</date><risdate>2009</risdate><volume>66</volume><issue>1</issue><spage>69</spage><epage>73</epage><pages>69-73</pages><issn>0098-8472</issn><eissn>1873-7307</eissn><coden>EEBODM</coden><abstract>We expressed the
AtMT2b gene under the 35 S cauliflower mosaic virus promoter in
Nicotiana tabacum (Sr1), using leaf disc transformation. Arsenite tolerance and uptake, as well as arsenite-induced phytochelatin (PC) accumulation in roots were measured in transgenic lines, and compared to untransformed (‘wild type’) tobacco. Measured after 5 days of exposure, arsenite tolerance was slightly but significantly decreased in the transgenic lines compared to wild type. The highest
AtMT2b expressing line exhibited a significantly decreased arsenic accumulation in roots, but an increased accumulation in shoots, while the total amount of arsenic taken up remained unchanged, suggesting that
AtMT2b expression enhanced the arsenic root to shoot transport. The same transformant line also exhibited a decreased rate of phytochelatin accumulation in the roots, but the phytochelatin-SH to As molar ratio was higher than in wild type, suggesting that the lower arsenite tolerance in the transformant lines was not due to a potential shortage of cysteine for PC synthesis, imposed by expression of the transgene.</abstract><cop>Amsterdam</cop><pub>Elsevier B.V</pub><doi>10.1016/j.envexpbot.2008.12.021</doi><tpages>5</tpages></addata></record> |
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| subjects | Arabidopsis Arabidopsis thaliana Arabidopsis thaliana metallothionein 2b Arsenite toxicity arsenites Biological and medical sciences Cauliflower mosaic virus chelating agents cysteine Fundamental and applied biological sciences. Psychology genes genetic transformation metal tolerance metallothionein Nicotiana tabacum Nicotiana tabacum SR1 Phytochelatins roots shoots tobacco transgenic plants translocation (plant physiology) uptake mechanisms |
| title | Expression of the Arabidopsis metallothionein 2b enhances arsenite sensitivity and root to shoot translocation in tobacco |
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