A lentiviral vaccine expressing KMP11-HASPB fusion protein increases immune response to Leishmania major in BALB/C
Hydrophilic acylated surface protein B (HASPB) is an immunogenic Leishmania -specific protein that antibodies are produced against it in the sera of Leishmania -infected individuals. Kinetoplastid membrane protein 11 (KMP11) is another Leishmania antigen and considered as the suitable candidate for...
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| Veröffentlicht in: | Parasitology research (1987) 2018-07, Vol.117 (7), p.2265-2273 |
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| container_title | Parasitology research (1987) |
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| creator | Mortazavidehkordi, Nahid Fallah, Ali Abdollahi, Abbas Kia, Vahid Khanahmad, Hossein Najafabadi, Zahra Ghayour Hashemi, Nooshin Estiri, Bahareh Roudbari, Zahra Najafi, Ali Farjadfar, Akbar Hejazi, Seyed Hossein |
| description | Hydrophilic acylated surface protein B (HASPB) is an immunogenic
Leishmania
-specific protein that antibodies are produced against it in the sera of
Leishmania
-infected individuals. Kinetoplastid membrane protein 11 (KMP11) is another
Leishmania
antigen and considered as the suitable candidate for vaccine development Leishmaniasis. It is a highly conserved surface protein expressed in both promastigotes and amastigotes. In this study, KMP11 and HASPB coding sequences were cloned into a pCDH-cGFP lentiviral vector as a fusion protein to be used as a DNA vaccine against
L. major
. The KMP11-HASPB fusion protein was successfully expressed as evidenced by RT-PCR and Western blot assays. The effect of the vaccine was determined by evaluating the level of IFN-γ, IL-10, IgG1, and IgG2a performed using ELISA as well as determining the parasite load after challenge with
L. major
in vaccinated mice. The results revealed that IFN-γ, IL-10, IgG1, and IgG2a significantly increased after vaccination using KMP11-HASPB-expressing lentiviruses in BALB/c mice. It is noteworthy that the level of IFN-γ and IgG2a was higher than that of IL-10 and IgG1, respectively, which indicates the activation Th1 cells, macrophages, and cellular immunity. Moreover, the parasite load in the spleen and lymph node of vaccinated mice after challenge was significantly lower than that of controls. |
| doi_str_mv | 10.1007/s00436-018-5915-6 |
| format | Article |
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Leishmania
-specific protein that antibodies are produced against it in the sera of
Leishmania
-infected individuals. Kinetoplastid membrane protein 11 (KMP11) is another
Leishmania
antigen and considered as the suitable candidate for vaccine development Leishmaniasis. It is a highly conserved surface protein expressed in both promastigotes and amastigotes. In this study, KMP11 and HASPB coding sequences were cloned into a pCDH-cGFP lentiviral vector as a fusion protein to be used as a DNA vaccine against
L. major
. The KMP11-HASPB fusion protein was successfully expressed as evidenced by RT-PCR and Western blot assays. The effect of the vaccine was determined by evaluating the level of IFN-γ, IL-10, IgG1, and IgG2a performed using ELISA as well as determining the parasite load after challenge with
L. major
in vaccinated mice. The results revealed that IFN-γ, IL-10, IgG1, and IgG2a significantly increased after vaccination using KMP11-HASPB-expressing lentiviruses in BALB/c mice. It is noteworthy that the level of IFN-γ and IgG2a was higher than that of IL-10 and IgG1, respectively, which indicates the activation Th1 cells, macrophages, and cellular immunity. Moreover, the parasite load in the spleen and lymph node of vaccinated mice after challenge was significantly lower than that of controls.</description><identifier>ISSN: 0932-0113</identifier><identifier>EISSN: 1432-1955</identifier><identifier>DOI: 10.1007/s00436-018-5915-6</identifier><identifier>PMID: 29845415</identifier><language>eng</language><publisher>Berlin/Heidelberg: Springer Berlin Heidelberg</publisher><subject>Amastigotes ; Animals ; Antibodies, Protozoan - blood ; Antibodies, Protozoan - immunology ; Antigens ; Antigens, Protozoan - genetics ; Antigens, Protozoan - immunology ; Biomedical and Life Sciences ; Biomedicine ; Cell activation ; Cell Line ; Cell-mediated immunity ; DNA vaccines ; Enzyme-linked immunosorbent assay ; Female ; Fusion protein ; HEK293 Cells ; Humans ; Immune response ; Immunogenicity ; Immunoglobulin G ; Immunoglobulin G - blood ; Immunology ; Interferon-gamma - blood ; Interleukin 10 ; Interleukin-10 - blood ; Leishmania ; Leishmania major - immunology ; Leishmaniasis ; Leishmaniasis - immunology ; Leishmaniasis - prevention & control ; Leishmaniasis Vaccines - immunology ; Lentivirus - genetics ; Lymph nodes ; Lymphocyte Activation - immunology ; Lymphocytes T ; Macrophage Activation - immunology ; Macrophages ; Macrophages - immunology ; Medical Microbiology ; Membrane proteins ; Membrane Proteins - genetics ; Membrane Proteins - immunology ; Mice ; Mice, Inbred BALB C ; Microbiology ; Original Paper ; Parasite Load ; Parasitic diseases ; Polymerase chain reaction ; Promastigotes ; Protein B ; Proteins ; Protozoan Proteins - genetics ; Protozoan Proteins - immunology ; Recombinant Fusion Proteins - genetics ; Recombinant Fusion Proteins - immunology ; Spleen ; Spleen - immunology ; Th1 Cells - immunology ; Vaccination ; Vaccine development ; Vaccines ; γ-Interferon</subject><ispartof>Parasitology research (1987), 2018-07, Vol.117 (7), p.2265-2273</ispartof><rights>Springer-Verlag GmbH Germany, part of Springer Nature 2018</rights><rights>COPYRIGHT 2018 Springer</rights><rights>Springer-Verlag GmbH Germany, part of Springer Nature 2018.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c482t-d6cedd03eaf6aa8382e7e67c8cc6a95b053ec5554b560ab3faf15455aa41e4013</citedby><cites>FETCH-LOGICAL-c482t-d6cedd03eaf6aa8382e7e67c8cc6a95b053ec5554b560ab3faf15455aa41e4013</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s00436-018-5915-6$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s00436-018-5915-6$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,776,780,27903,27904,41467,42536,51298</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/29845415$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Mortazavidehkordi, Nahid</creatorcontrib><creatorcontrib>Fallah, Ali</creatorcontrib><creatorcontrib>Abdollahi, Abbas</creatorcontrib><creatorcontrib>Kia, Vahid</creatorcontrib><creatorcontrib>Khanahmad, Hossein</creatorcontrib><creatorcontrib>Najafabadi, Zahra Ghayour</creatorcontrib><creatorcontrib>Hashemi, Nooshin</creatorcontrib><creatorcontrib>Estiri, Bahareh</creatorcontrib><creatorcontrib>Roudbari, Zahra</creatorcontrib><creatorcontrib>Najafi, Ali</creatorcontrib><creatorcontrib>Farjadfar, Akbar</creatorcontrib><creatorcontrib>Hejazi, Seyed Hossein</creatorcontrib><title>A lentiviral vaccine expressing KMP11-HASPB fusion protein increases immune response to Leishmania major in BALB/C</title><title>Parasitology research (1987)</title><addtitle>Parasitol Res</addtitle><addtitle>Parasitol Res</addtitle><description>Hydrophilic acylated surface protein B (HASPB) is an immunogenic
Leishmania
-specific protein that antibodies are produced against it in the sera of
Leishmania
-infected individuals. Kinetoplastid membrane protein 11 (KMP11) is another
Leishmania
antigen and considered as the suitable candidate for vaccine development Leishmaniasis. It is a highly conserved surface protein expressed in both promastigotes and amastigotes. In this study, KMP11 and HASPB coding sequences were cloned into a pCDH-cGFP lentiviral vector as a fusion protein to be used as a DNA vaccine against
L. major
. The KMP11-HASPB fusion protein was successfully expressed as evidenced by RT-PCR and Western blot assays. The effect of the vaccine was determined by evaluating the level of IFN-γ, IL-10, IgG1, and IgG2a performed using ELISA as well as determining the parasite load after challenge with
L. major
in vaccinated mice. The results revealed that IFN-γ, IL-10, IgG1, and IgG2a significantly increased after vaccination using KMP11-HASPB-expressing lentiviruses in BALB/c mice. It is noteworthy that the level of IFN-γ and IgG2a was higher than that of IL-10 and IgG1, respectively, which indicates the activation Th1 cells, macrophages, and cellular immunity. Moreover, the parasite load in the spleen and lymph node of vaccinated mice after challenge was significantly lower than that of controls.</description><subject>Amastigotes</subject><subject>Animals</subject><subject>Antibodies, Protozoan - blood</subject><subject>Antibodies, Protozoan - immunology</subject><subject>Antigens</subject><subject>Antigens, Protozoan - genetics</subject><subject>Antigens, Protozoan - immunology</subject><subject>Biomedical and Life Sciences</subject><subject>Biomedicine</subject><subject>Cell activation</subject><subject>Cell Line</subject><subject>Cell-mediated immunity</subject><subject>DNA vaccines</subject><subject>Enzyme-linked immunosorbent assay</subject><subject>Female</subject><subject>Fusion protein</subject><subject>HEK293 Cells</subject><subject>Humans</subject><subject>Immune response</subject><subject>Immunogenicity</subject><subject>Immunoglobulin G</subject><subject>Immunoglobulin G - blood</subject><subject>Immunology</subject><subject>Interferon-gamma - blood</subject><subject>Interleukin 10</subject><subject>Interleukin-10 - blood</subject><subject>Leishmania</subject><subject>Leishmania major - immunology</subject><subject>Leishmaniasis</subject><subject>Leishmaniasis - immunology</subject><subject>Leishmaniasis - prevention & control</subject><subject>Leishmaniasis Vaccines - immunology</subject><subject>Lentivirus - genetics</subject><subject>Lymph nodes</subject><subject>Lymphocyte Activation - immunology</subject><subject>Lymphocytes T</subject><subject>Macrophage Activation - immunology</subject><subject>Macrophages</subject><subject>Macrophages - immunology</subject><subject>Medical Microbiology</subject><subject>Membrane proteins</subject><subject>Membrane Proteins - genetics</subject><subject>Membrane Proteins - immunology</subject><subject>Mice</subject><subject>Mice, Inbred BALB C</subject><subject>Microbiology</subject><subject>Original Paper</subject><subject>Parasite Load</subject><subject>Parasitic diseases</subject><subject>Polymerase chain reaction</subject><subject>Promastigotes</subject><subject>Protein B</subject><subject>Proteins</subject><subject>Protozoan Proteins - genetics</subject><subject>Protozoan Proteins - immunology</subject><subject>Recombinant Fusion Proteins - genetics</subject><subject>Recombinant Fusion Proteins - immunology</subject><subject>Spleen</subject><subject>Spleen - immunology</subject><subject>Th1 Cells - immunology</subject><subject>Vaccination</subject><subject>Vaccine development</subject><subject>Vaccines</subject><subject>γ-Interferon</subject><issn>0932-0113</issn><issn>1432-1955</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2018</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1kdFu0zAUhi0EYmXwANwgS9xwk80nsZ3ksq0YQxQxCbi2Tp2T4iqxi51M8PZz1cEEAvnClv19v471M_YSxAUIUV8mIWSlCwFNoVpQhX7EFiCrsoBWqcdsIdp8FgDVGXuW0l4IqLWUT9lZ2TZSSVALFpd8ID-5Wxdx4LdorfPE6cchUkrO7_iHjzcAxfXy882K93NywfNDDBM5z523kTBR4m4c56xl5xB8Ij4FviGXvo3oHfIR9yFmmq-Wm9Xl-jl70uOQ6MX9fs6-Xr39sr4uNp_evV8vN4WVTTkVnbbUdaIi7DViUzUl1aRr21irsVVboSqySim5VVrgtuqxByWVQpRAUkB1zt6ccvO832dKkxldsjQM6CnMyZRC1mXT1kpm9PVf6D7M0efpMqV03YJu4IHa4UDG-T5MEe0x1CxziAJVliJTF_-g8upodDZ46l2-_0OAk2BjSClSbw7RjRh_GhDm2LM59Wxyz-bYs9HZeXU_8Lwdqftt_Co2A-UJSPnJ7yg-_Oj_qXfca7B-</recordid><startdate>20180701</startdate><enddate>20180701</enddate><creator>Mortazavidehkordi, Nahid</creator><creator>Fallah, Ali</creator><creator>Abdollahi, Abbas</creator><creator>Kia, Vahid</creator><creator>Khanahmad, Hossein</creator><creator>Najafabadi, Zahra Ghayour</creator><creator>Hashemi, Nooshin</creator><creator>Estiri, Bahareh</creator><creator>Roudbari, Zahra</creator><creator>Najafi, Ali</creator><creator>Farjadfar, Akbar</creator><creator>Hejazi, Seyed Hossein</creator><general>Springer Berlin Heidelberg</general><general>Springer</general><general>Springer Nature B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20180701</creationdate><title>A lentiviral vaccine expressing KMP11-HASPB fusion protein increases immune response to Leishmania major in BALB/C</title><author>Mortazavidehkordi, Nahid ; Fallah, Ali ; Abdollahi, Abbas ; Kia, Vahid ; Khanahmad, Hossein ; Najafabadi, Zahra Ghayour ; Hashemi, Nooshin ; Estiri, Bahareh ; Roudbari, Zahra ; Najafi, Ali ; Farjadfar, Akbar ; Hejazi, Seyed Hossein</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c482t-d6cedd03eaf6aa8382e7e67c8cc6a95b053ec5554b560ab3faf15455aa41e4013</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2018</creationdate><topic>Amastigotes</topic><topic>Animals</topic><topic>Antibodies, Protozoan - blood</topic><topic>Antibodies, Protozoan - immunology</topic><topic>Antigens</topic><topic>Antigens, Protozoan - genetics</topic><topic>Antigens, Protozoan - immunology</topic><topic>Biomedical and Life Sciences</topic><topic>Biomedicine</topic><topic>Cell activation</topic><topic>Cell Line</topic><topic>Cell-mediated immunity</topic><topic>DNA vaccines</topic><topic>Enzyme-linked immunosorbent assay</topic><topic>Female</topic><topic>Fusion protein</topic><topic>HEK293 Cells</topic><topic>Humans</topic><topic>Immune response</topic><topic>Immunogenicity</topic><topic>Immunoglobulin G</topic><topic>Immunoglobulin G - blood</topic><topic>Immunology</topic><topic>Interferon-gamma - blood</topic><topic>Interleukin 10</topic><topic>Interleukin-10 - blood</topic><topic>Leishmania</topic><topic>Leishmania major - immunology</topic><topic>Leishmaniasis</topic><topic>Leishmaniasis - immunology</topic><topic>Leishmaniasis - prevention & control</topic><topic>Leishmaniasis Vaccines - immunology</topic><topic>Lentivirus - genetics</topic><topic>Lymph nodes</topic><topic>Lymphocyte Activation - immunology</topic><topic>Lymphocytes T</topic><topic>Macrophage Activation - immunology</topic><topic>Macrophages</topic><topic>Macrophages - immunology</topic><topic>Medical Microbiology</topic><topic>Membrane proteins</topic><topic>Membrane Proteins - genetics</topic><topic>Membrane Proteins - immunology</topic><topic>Mice</topic><topic>Mice, Inbred BALB C</topic><topic>Microbiology</topic><topic>Original Paper</topic><topic>Parasite Load</topic><topic>Parasitic diseases</topic><topic>Polymerase chain reaction</topic><topic>Promastigotes</topic><topic>Protein B</topic><topic>Proteins</topic><topic>Protozoan Proteins - genetics</topic><topic>Protozoan Proteins - immunology</topic><topic>Recombinant Fusion Proteins - genetics</topic><topic>Recombinant Fusion Proteins - immunology</topic><topic>Spleen</topic><topic>Spleen - immunology</topic><topic>Th1 Cells - immunology</topic><topic>Vaccination</topic><topic>Vaccine development</topic><topic>Vaccines</topic><topic>γ-Interferon</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Mortazavidehkordi, Nahid</creatorcontrib><creatorcontrib>Fallah, Ali</creatorcontrib><creatorcontrib>Abdollahi, Abbas</creatorcontrib><creatorcontrib>Kia, Vahid</creatorcontrib><creatorcontrib>Khanahmad, Hossein</creatorcontrib><creatorcontrib>Najafabadi, Zahra Ghayour</creatorcontrib><creatorcontrib>Hashemi, Nooshin</creatorcontrib><creatorcontrib>Estiri, Bahareh</creatorcontrib><creatorcontrib>Roudbari, Zahra</creatorcontrib><creatorcontrib>Najafi, Ali</creatorcontrib><creatorcontrib>Farjadfar, Akbar</creatorcontrib><creatorcontrib>Hejazi, Seyed Hossein</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Parasitology research (1987)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Mortazavidehkordi, Nahid</au><au>Fallah, Ali</au><au>Abdollahi, Abbas</au><au>Kia, Vahid</au><au>Khanahmad, Hossein</au><au>Najafabadi, Zahra Ghayour</au><au>Hashemi, Nooshin</au><au>Estiri, Bahareh</au><au>Roudbari, Zahra</au><au>Najafi, Ali</au><au>Farjadfar, Akbar</au><au>Hejazi, Seyed Hossein</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A lentiviral vaccine expressing KMP11-HASPB fusion protein increases immune response to Leishmania major in BALB/C</atitle><jtitle>Parasitology research (1987)</jtitle><stitle>Parasitol Res</stitle><addtitle>Parasitol Res</addtitle><date>2018-07-01</date><risdate>2018</risdate><volume>117</volume><issue>7</issue><spage>2265</spage><epage>2273</epage><pages>2265-2273</pages><issn>0932-0113</issn><eissn>1432-1955</eissn><abstract>Hydrophilic acylated surface protein B (HASPB) is an immunogenic
Leishmania
-specific protein that antibodies are produced against it in the sera of
Leishmania
-infected individuals. Kinetoplastid membrane protein 11 (KMP11) is another
Leishmania
antigen and considered as the suitable candidate for vaccine development Leishmaniasis. It is a highly conserved surface protein expressed in both promastigotes and amastigotes. In this study, KMP11 and HASPB coding sequences were cloned into a pCDH-cGFP lentiviral vector as a fusion protein to be used as a DNA vaccine against
L. major
. The KMP11-HASPB fusion protein was successfully expressed as evidenced by RT-PCR and Western blot assays. The effect of the vaccine was determined by evaluating the level of IFN-γ, IL-10, IgG1, and IgG2a performed using ELISA as well as determining the parasite load after challenge with
L. major
in vaccinated mice. The results revealed that IFN-γ, IL-10, IgG1, and IgG2a significantly increased after vaccination using KMP11-HASPB-expressing lentiviruses in BALB/c mice. It is noteworthy that the level of IFN-γ and IgG2a was higher than that of IL-10 and IgG1, respectively, which indicates the activation Th1 cells, macrophages, and cellular immunity. Moreover, the parasite load in the spleen and lymph node of vaccinated mice after challenge was significantly lower than that of controls.</abstract><cop>Berlin/Heidelberg</cop><pub>Springer Berlin Heidelberg</pub><pmid>29845415</pmid><doi>10.1007/s00436-018-5915-6</doi><tpages>9</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0932-0113 |
| ispartof | Parasitology research (1987), 2018-07, Vol.117 (7), p.2265-2273 |
| issn | 0932-0113 1432-1955 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_2047289754 |
| source | MEDLINE; Springer Nature - Complete Springer Journals |
| subjects | Amastigotes Animals Antibodies, Protozoan - blood Antibodies, Protozoan - immunology Antigens Antigens, Protozoan - genetics Antigens, Protozoan - immunology Biomedical and Life Sciences Biomedicine Cell activation Cell Line Cell-mediated immunity DNA vaccines Enzyme-linked immunosorbent assay Female Fusion protein HEK293 Cells Humans Immune response Immunogenicity Immunoglobulin G Immunoglobulin G - blood Immunology Interferon-gamma - blood Interleukin 10 Interleukin-10 - blood Leishmania Leishmania major - immunology Leishmaniasis Leishmaniasis - immunology Leishmaniasis - prevention & control Leishmaniasis Vaccines - immunology Lentivirus - genetics Lymph nodes Lymphocyte Activation - immunology Lymphocytes T Macrophage Activation - immunology Macrophages Macrophages - immunology Medical Microbiology Membrane proteins Membrane Proteins - genetics Membrane Proteins - immunology Mice Mice, Inbred BALB C Microbiology Original Paper Parasite Load Parasitic diseases Polymerase chain reaction Promastigotes Protein B Proteins Protozoan Proteins - genetics Protozoan Proteins - immunology Recombinant Fusion Proteins - genetics Recombinant Fusion Proteins - immunology Spleen Spleen - immunology Th1 Cells - immunology Vaccination Vaccine development Vaccines γ-Interferon |
| title | A lentiviral vaccine expressing KMP11-HASPB fusion protein increases immune response to Leishmania major in BALB/C |
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