Enhancement of glutathione production in a coupled system of adenosine deaminase-deficient recombinant Escherichia coli and Saccharomyces cerevisiae
Adenosine triphosphate (ATP) is necessary in the enzymatic production of glutathione (GSH). Our aim was to improve GSH production by increasing the efficiency of ATP regeneration in a coupled system. Previous results suggested that low GSH production in the coupled system is due to the irreversible...
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| Veröffentlicht in: | Enzyme and microbial technology 2009-05, Vol.44 (5), p.269-273 |
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| creator | Lin, Jun Liao, Xianyan Du, Guocheng Chen, Jian |
| description | Adenosine triphosphate (ATP) is necessary in the enzymatic production of glutathione (GSH). Our aim was to improve GSH production by increasing the efficiency of ATP regeneration in a coupled system. Previous results suggested that low GSH production in the coupled system is due to the irreversible transformation of adenosine (Ado) to hypoxanthine (Hx) via inosine (Ino) pathway in
Escherichia coli JM109 (pBV03). In this study, to block the transformation of Ado into Hx and enhance GSH production, a coupled ATP regeneration system was constructed with adenosine deaminase-deficient recombinant
E. coli JW1615 (pBV03) and
Saccharomyces cerevisiae WSH2. GSH production was improved (2.94-fold of the control), and ATP regeneration reaction was established in the coupled system. The results are applicable to ATP regeneration in other microbial processes. |
| doi_str_mv | 10.1016/j.enzmictec.2009.01.003 |
| format | Article |
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Escherichia coli JM109 (pBV03). In this study, to block the transformation of Ado into Hx and enhance GSH production, a coupled ATP regeneration system was constructed with adenosine deaminase-deficient recombinant
E. coli JW1615 (pBV03) and
Saccharomyces cerevisiae WSH2. GSH production was improved (2.94-fold of the control), and ATP regeneration reaction was established in the coupled system. The results are applicable to ATP regeneration in other microbial processes.</description><identifier>ISSN: 0141-0229</identifier><identifier>EISSN: 1879-0909</identifier><identifier>DOI: 10.1016/j.enzmictec.2009.01.003</identifier><identifier>CODEN: EMTED2</identifier><language>eng</language><publisher>Amsterdam: Elsevier Inc</publisher><subject>Adenine deaminase ; Adenosine deaminase ; ATP ; Biological and medical sciences ; Biotechnology ; Escherichia coli ; Fundamental and applied biological sciences. Psychology ; Glutathione production ; Saccharomyces cerevisiae</subject><ispartof>Enzyme and microbial technology, 2009-05, Vol.44 (5), p.269-273</ispartof><rights>2009 Elsevier Inc.</rights><rights>2009 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c425t-87315a291ec14c3ab22163bcf2a7be2db8c180766259f0f5d4e927aedb934aa43</citedby><cites>FETCH-LOGICAL-c425t-87315a291ec14c3ab22163bcf2a7be2db8c180766259f0f5d4e927aedb934aa43</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.enzmictec.2009.01.003$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=21326472$$DView record in Pascal Francis$$Hfree_for_read</backlink></links><search><creatorcontrib>Lin, Jun</creatorcontrib><creatorcontrib>Liao, Xianyan</creatorcontrib><creatorcontrib>Du, Guocheng</creatorcontrib><creatorcontrib>Chen, Jian</creatorcontrib><title>Enhancement of glutathione production in a coupled system of adenosine deaminase-deficient recombinant Escherichia coli and Saccharomyces cerevisiae</title><title>Enzyme and microbial technology</title><description>Adenosine triphosphate (ATP) is necessary in the enzymatic production of glutathione (GSH). Our aim was to improve GSH production by increasing the efficiency of ATP regeneration in a coupled system. Previous results suggested that low GSH production in the coupled system is due to the irreversible transformation of adenosine (Ado) to hypoxanthine (Hx) via inosine (Ino) pathway in
Escherichia coli JM109 (pBV03). In this study, to block the transformation of Ado into Hx and enhance GSH production, a coupled ATP regeneration system was constructed with adenosine deaminase-deficient recombinant
E. coli JW1615 (pBV03) and
Saccharomyces cerevisiae WSH2. GSH production was improved (2.94-fold of the control), and ATP regeneration reaction was established in the coupled system. The results are applicable to ATP regeneration in other microbial processes.</description><subject>Adenine deaminase</subject><subject>Adenosine deaminase</subject><subject>ATP</subject><subject>Biological and medical sciences</subject><subject>Biotechnology</subject><subject>Escherichia coli</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Glutathione production</subject><subject>Saccharomyces cerevisiae</subject><issn>0141-0229</issn><issn>1879-0909</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2009</creationdate><recordtype>article</recordtype><recordid>eNqFkcGO1DAMhisEEsPCM5AL3FqctNNOjqvVsCCtxAE4R67j0ozaZEjalYbn4IFJNau9crJlff4t_39RvJdQSZDtp1PF_s_saGGqFICuQFYA9YtiJw-dLkGDflnsQDayBKX06-JNSieAPGhgV_w9-hE98cx-EWEQv6Z1wWV0wbM4x2BXWnIvnBcoKKznia1Il7TwvNFo2YfkMmsZZ-cxcWl5cOQ2ucgU5j5Pc39MNHJ0NLpNZ3ICvRXfkWjEGOYLcRLEkR9dcshvi1cDTonfPdWb4ufn44-7L-XDt_uvd7cPJTVqv5SHrpZ7VFoyyYZq7JWSbd3ToLDrWdn-QPIAXduqvR5g2NuGteqQba_rBrGpb4qPV9386e-V02Jml4inCT2HNRkFTQdStxnsriDFkFLkwZyjmzFejASzpWBO5jkFs6VgQJqcQt788HQCE-E0xGy2S8_rStaqbTqVudsrx_nfR8fRpM1EYuuyjYuxwf331j-GYqY1</recordid><startdate>20090506</startdate><enddate>20090506</enddate><creator>Lin, Jun</creator><creator>Liao, Xianyan</creator><creator>Du, Guocheng</creator><creator>Chen, Jian</creator><general>Elsevier Inc</general><general>Elsevier</general><scope>IQODW</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7QO</scope><scope>7T7</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>M7N</scope><scope>P64</scope></search><sort><creationdate>20090506</creationdate><title>Enhancement of glutathione production in a coupled system of adenosine deaminase-deficient recombinant Escherichia coli and Saccharomyces cerevisiae</title><author>Lin, Jun ; Liao, Xianyan ; Du, Guocheng ; Chen, Jian</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c425t-87315a291ec14c3ab22163bcf2a7be2db8c180766259f0f5d4e927aedb934aa43</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2009</creationdate><topic>Adenine deaminase</topic><topic>Adenosine deaminase</topic><topic>ATP</topic><topic>Biological and medical sciences</topic><topic>Biotechnology</topic><topic>Escherichia coli</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Glutathione production</topic><topic>Saccharomyces cerevisiae</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Lin, Jun</creatorcontrib><creatorcontrib>Liao, Xianyan</creatorcontrib><creatorcontrib>Du, Guocheng</creatorcontrib><creatorcontrib>Chen, Jian</creatorcontrib><collection>Pascal-Francis</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Biotechnology Research Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><jtitle>Enzyme and microbial technology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Lin, Jun</au><au>Liao, Xianyan</au><au>Du, Guocheng</au><au>Chen, Jian</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Enhancement of glutathione production in a coupled system of adenosine deaminase-deficient recombinant Escherichia coli and Saccharomyces cerevisiae</atitle><jtitle>Enzyme and microbial technology</jtitle><date>2009-05-06</date><risdate>2009</risdate><volume>44</volume><issue>5</issue><spage>269</spage><epage>273</epage><pages>269-273</pages><issn>0141-0229</issn><eissn>1879-0909</eissn><coden>EMTED2</coden><abstract>Adenosine triphosphate (ATP) is necessary in the enzymatic production of glutathione (GSH). Our aim was to improve GSH production by increasing the efficiency of ATP regeneration in a coupled system. Previous results suggested that low GSH production in the coupled system is due to the irreversible transformation of adenosine (Ado) to hypoxanthine (Hx) via inosine (Ino) pathway in
Escherichia coli JM109 (pBV03). In this study, to block the transformation of Ado into Hx and enhance GSH production, a coupled ATP regeneration system was constructed with adenosine deaminase-deficient recombinant
E. coli JW1615 (pBV03) and
Saccharomyces cerevisiae WSH2. GSH production was improved (2.94-fold of the control), and ATP regeneration reaction was established in the coupled system. The results are applicable to ATP regeneration in other microbial processes.</abstract><cop>Amsterdam</cop><pub>Elsevier Inc</pub><doi>10.1016/j.enzmictec.2009.01.003</doi><tpages>5</tpages><oa>free_for_read</oa></addata></record> |
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| identifier | ISSN: 0141-0229 |
| ispartof | Enzyme and microbial technology, 2009-05, Vol.44 (5), p.269-273 |
| issn | 0141-0229 1879-0909 |
| language | eng |
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| source | Elsevier ScienceDirect Journals Complete |
| subjects | Adenine deaminase Adenosine deaminase ATP Biological and medical sciences Biotechnology Escherichia coli Fundamental and applied biological sciences. Psychology Glutathione production Saccharomyces cerevisiae |
| title | Enhancement of glutathione production in a coupled system of adenosine deaminase-deficient recombinant Escherichia coli and Saccharomyces cerevisiae |
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