Oral absorption, tissue distribution and excretion of a radiolabelled analog of a milk-derived antihypertensive peptide, Ile-Pro-Pro, in rats
Milk-derived bioactive peptides have been shown to attenuate the development of hypertension in rats and reduce blood pressure in humans. The aim of the present study was to examine the absorption, tissue distribution and excretion of intact Ile-Pro-[ 14C]Pro in rats. Radioactivity was determined in...
Gespeichert in:
| Veröffentlicht in: | International dairy journal 2007-10, Vol.17 (10), p.1216-1223 |
|---|---|
| Hauptverfasser: | , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 1223 |
|---|---|
| container_issue | 10 |
| container_start_page | 1216 |
| container_title | International dairy journal |
| container_volume | 17 |
| creator | Jauhiainen, Tiina Wuolle, Katariina Vapaatalo, Heikki Kerojoki, Outi Nurmela, Kari Lowrie, Chris Korpela, Riitta |
| description | Milk-derived bioactive peptides have been shown to attenuate the development of hypertension in rats and reduce blood pressure in humans. The aim of the present study was to examine the absorption, tissue distribution and excretion of intact Ile-Pro-[
14C]Pro in rats. Radioactivity was determined in blood and tissues. After oral administration, Ile-Pro-[
14C]Pro was absorbed from the intestine and the peak level was observed after 2
h. Considerable amounts of radioactivity were found by liquid scintillation in tissues, e.g., in the liver, kidneys, adrenals and aorta. Chromatographic and mass spectrometric analyses of plasma samples proved that Ile-Pro-[
14C]Pro was present in all samples analysed. The majority of the radioactivity was associated with plasma proteins and
in vitro tests showed that Pro-Pro attaches to plasma proteins (over 98%) whereas Ile-Pro-Pro is not bound. In conclusion, Ile-Pro-[
14C]Pro is partly absorbed intact and the distribution of radioactivity in tissues may be related to the blood-pressure-lowering effect of the peptide. |
| doi_str_mv | 10.1016/j.idairyj.2007.02.004 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_20469067</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>S0958694607000404</els_id><sourcerecordid>20469067</sourcerecordid><originalsourceid>FETCH-LOGICAL-c460t-4ae12208cce2a87a9ac623c926f8c4feb26596f261addfec9d7cd81e44265cf33</originalsourceid><addsrcrecordid>eNqFkM9u1DAQxiMEUpfCI1T4AqdNGDtZJz4hVPGnUqUilZ6tWXtcvGTjYGer7kP0nXGalThysCzP95tvxl9RXHCoOHD5cVd5iz4ed5UAaCsQFUDzoljxrlUl8KZ-WaxAbbpSqkaeFa9T2gHwFmq1Kp5uIvYMtynEcfJhWLPJp3QgZn2aot8e5iLDwTJ6NJGeX8ExZBGtDz1uqe_JZgD7cL8oe9__Li1F__AsTP7XcaQ40ZByhY2U51has6ueyh8xzGfN_JANp_SmeOWwT_T2dJ8Xd1-__Lz8Xl7ffLu6_HxdmkbCVDZIXAjojCGBXYsKjRS1UUK6zjSOtkJulHRCcrTWkVG2Nbbj1DRZMK6uz4sPi-8Yw58DpUnvfTL5KzhQOCQtoJEKZJvBzQKaGFKK5PQY_R7jUXPQc_h6p0_h6zl8DULn8HPf-9MATAZ7F3EwPv1r7lT2r-dF3i2cw6DxPmbm7lYArwE6XivJM_FpISjn8eAp6mQ8DYasj2QmbYP_zy5_AZs5qZk</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>20469067</pqid></control><display><type>article</type><title>Oral absorption, tissue distribution and excretion of a radiolabelled analog of a milk-derived antihypertensive peptide, Ile-Pro-Pro, in rats</title><source>Elsevier ScienceDirect Journals</source><creator>Jauhiainen, Tiina ; Wuolle, Katariina ; Vapaatalo, Heikki ; Kerojoki, Outi ; Nurmela, Kari ; Lowrie, Chris ; Korpela, Riitta</creator><creatorcontrib>Jauhiainen, Tiina ; Wuolle, Katariina ; Vapaatalo, Heikki ; Kerojoki, Outi ; Nurmela, Kari ; Lowrie, Chris ; Korpela, Riitta</creatorcontrib><description>Milk-derived bioactive peptides have been shown to attenuate the development of hypertension in rats and reduce blood pressure in humans. The aim of the present study was to examine the absorption, tissue distribution and excretion of intact Ile-Pro-[
14C]Pro in rats. Radioactivity was determined in blood and tissues. After oral administration, Ile-Pro-[
14C]Pro was absorbed from the intestine and the peak level was observed after 2
h. Considerable amounts of radioactivity were found by liquid scintillation in tissues, e.g., in the liver, kidneys, adrenals and aorta. Chromatographic and mass spectrometric analyses of plasma samples proved that Ile-Pro-[
14C]Pro was present in all samples analysed. The majority of the radioactivity was associated with plasma proteins and
in vitro tests showed that Pro-Pro attaches to plasma proteins (over 98%) whereas Ile-Pro-Pro is not bound. In conclusion, Ile-Pro-[
14C]Pro is partly absorbed intact and the distribution of radioactivity in tissues may be related to the blood-pressure-lowering effect of the peptide.</description><identifier>ISSN: 0958-6946</identifier><identifier>EISSN: 1879-0143</identifier><identifier>DOI: 10.1016/j.idairyj.2007.02.004</identifier><language>eng</language><publisher>Oxford: Elsevier Ltd</publisher><subject>Absorption ; animal models ; antihypertensive agents ; bioactive properties ; Biological and medical sciences ; excretion ; Food industries ; Fundamental and applied biological sciences. Psychology ; human nutrition ; Ile-Pro-Pro ; Kinetics ; milk ; Milk and cheese industries. Ice creams ; Milk-derived peptides ; oral administration ; Radioactive label ; radiolabeling ; rats ; tissue distribution ; tripeptides</subject><ispartof>International dairy journal, 2007-10, Vol.17 (10), p.1216-1223</ispartof><rights>2007 Elsevier Ltd</rights><rights>2007 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c460t-4ae12208cce2a87a9ac623c926f8c4feb26596f261addfec9d7cd81e44265cf33</citedby><cites>FETCH-LOGICAL-c460t-4ae12208cce2a87a9ac623c926f8c4feb26596f261addfec9d7cd81e44265cf33</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0958694607000404$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3536,27903,27904,65309</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=18969033$$DView record in Pascal Francis$$Hfree_for_read</backlink></links><search><creatorcontrib>Jauhiainen, Tiina</creatorcontrib><creatorcontrib>Wuolle, Katariina</creatorcontrib><creatorcontrib>Vapaatalo, Heikki</creatorcontrib><creatorcontrib>Kerojoki, Outi</creatorcontrib><creatorcontrib>Nurmela, Kari</creatorcontrib><creatorcontrib>Lowrie, Chris</creatorcontrib><creatorcontrib>Korpela, Riitta</creatorcontrib><title>Oral absorption, tissue distribution and excretion of a radiolabelled analog of a milk-derived antihypertensive peptide, Ile-Pro-Pro, in rats</title><title>International dairy journal</title><description>Milk-derived bioactive peptides have been shown to attenuate the development of hypertension in rats and reduce blood pressure in humans. The aim of the present study was to examine the absorption, tissue distribution and excretion of intact Ile-Pro-[
14C]Pro in rats. Radioactivity was determined in blood and tissues. After oral administration, Ile-Pro-[
14C]Pro was absorbed from the intestine and the peak level was observed after 2
h. Considerable amounts of radioactivity were found by liquid scintillation in tissues, e.g., in the liver, kidneys, adrenals and aorta. Chromatographic and mass spectrometric analyses of plasma samples proved that Ile-Pro-[
14C]Pro was present in all samples analysed. The majority of the radioactivity was associated with plasma proteins and
in vitro tests showed that Pro-Pro attaches to plasma proteins (over 98%) whereas Ile-Pro-Pro is not bound. In conclusion, Ile-Pro-[
14C]Pro is partly absorbed intact and the distribution of radioactivity in tissues may be related to the blood-pressure-lowering effect of the peptide.</description><subject>Absorption</subject><subject>animal models</subject><subject>antihypertensive agents</subject><subject>bioactive properties</subject><subject>Biological and medical sciences</subject><subject>excretion</subject><subject>Food industries</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>human nutrition</subject><subject>Ile-Pro-Pro</subject><subject>Kinetics</subject><subject>milk</subject><subject>Milk and cheese industries. Ice creams</subject><subject>Milk-derived peptides</subject><subject>oral administration</subject><subject>Radioactive label</subject><subject>radiolabeling</subject><subject>rats</subject><subject>tissue distribution</subject><subject>tripeptides</subject><issn>0958-6946</issn><issn>1879-0143</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2007</creationdate><recordtype>article</recordtype><recordid>eNqFkM9u1DAQxiMEUpfCI1T4AqdNGDtZJz4hVPGnUqUilZ6tWXtcvGTjYGer7kP0nXGalThysCzP95tvxl9RXHCoOHD5cVd5iz4ed5UAaCsQFUDzoljxrlUl8KZ-WaxAbbpSqkaeFa9T2gHwFmq1Kp5uIvYMtynEcfJhWLPJp3QgZn2aot8e5iLDwTJ6NJGeX8ExZBGtDz1uqe_JZgD7cL8oe9__Li1F__AsTP7XcaQ40ZByhY2U51has6ueyh8xzGfN_JANp_SmeOWwT_T2dJ8Xd1-__Lz8Xl7ffLu6_HxdmkbCVDZIXAjojCGBXYsKjRS1UUK6zjSOtkJulHRCcrTWkVG2Nbbj1DRZMK6uz4sPi-8Yw58DpUnvfTL5KzhQOCQtoJEKZJvBzQKaGFKK5PQY_R7jUXPQc_h6p0_h6zl8DULn8HPf-9MATAZ7F3EwPv1r7lT2r-dF3i2cw6DxPmbm7lYArwE6XivJM_FpISjn8eAp6mQ8DYasj2QmbYP_zy5_AZs5qZk</recordid><startdate>20071001</startdate><enddate>20071001</enddate><creator>Jauhiainen, Tiina</creator><creator>Wuolle, Katariina</creator><creator>Vapaatalo, Heikki</creator><creator>Kerojoki, Outi</creator><creator>Nurmela, Kari</creator><creator>Lowrie, Chris</creator><creator>Korpela, Riitta</creator><general>Elsevier Ltd</general><general>Elsevier Science</general><scope>FBQ</scope><scope>IQODW</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope></search><sort><creationdate>20071001</creationdate><title>Oral absorption, tissue distribution and excretion of a radiolabelled analog of a milk-derived antihypertensive peptide, Ile-Pro-Pro, in rats</title><author>Jauhiainen, Tiina ; Wuolle, Katariina ; Vapaatalo, Heikki ; Kerojoki, Outi ; Nurmela, Kari ; Lowrie, Chris ; Korpela, Riitta</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c460t-4ae12208cce2a87a9ac623c926f8c4feb26596f261addfec9d7cd81e44265cf33</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2007</creationdate><topic>Absorption</topic><topic>animal models</topic><topic>antihypertensive agents</topic><topic>bioactive properties</topic><topic>Biological and medical sciences</topic><topic>excretion</topic><topic>Food industries</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>human nutrition</topic><topic>Ile-Pro-Pro</topic><topic>Kinetics</topic><topic>milk</topic><topic>Milk and cheese industries. Ice creams</topic><topic>Milk-derived peptides</topic><topic>oral administration</topic><topic>Radioactive label</topic><topic>radiolabeling</topic><topic>rats</topic><topic>tissue distribution</topic><topic>tripeptides</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Jauhiainen, Tiina</creatorcontrib><creatorcontrib>Wuolle, Katariina</creatorcontrib><creatorcontrib>Vapaatalo, Heikki</creatorcontrib><creatorcontrib>Kerojoki, Outi</creatorcontrib><creatorcontrib>Nurmela, Kari</creatorcontrib><creatorcontrib>Lowrie, Chris</creatorcontrib><creatorcontrib>Korpela, Riitta</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>CrossRef</collection><collection>Biotechnology Research Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><jtitle>International dairy journal</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Jauhiainen, Tiina</au><au>Wuolle, Katariina</au><au>Vapaatalo, Heikki</au><au>Kerojoki, Outi</au><au>Nurmela, Kari</au><au>Lowrie, Chris</au><au>Korpela, Riitta</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Oral absorption, tissue distribution and excretion of a radiolabelled analog of a milk-derived antihypertensive peptide, Ile-Pro-Pro, in rats</atitle><jtitle>International dairy journal</jtitle><date>2007-10-01</date><risdate>2007</risdate><volume>17</volume><issue>10</issue><spage>1216</spage><epage>1223</epage><pages>1216-1223</pages><issn>0958-6946</issn><eissn>1879-0143</eissn><abstract>Milk-derived bioactive peptides have been shown to attenuate the development of hypertension in rats and reduce blood pressure in humans. The aim of the present study was to examine the absorption, tissue distribution and excretion of intact Ile-Pro-[
14C]Pro in rats. Radioactivity was determined in blood and tissues. After oral administration, Ile-Pro-[
14C]Pro was absorbed from the intestine and the peak level was observed after 2
h. Considerable amounts of radioactivity were found by liquid scintillation in tissues, e.g., in the liver, kidneys, adrenals and aorta. Chromatographic and mass spectrometric analyses of plasma samples proved that Ile-Pro-[
14C]Pro was present in all samples analysed. The majority of the radioactivity was associated with plasma proteins and
in vitro tests showed that Pro-Pro attaches to plasma proteins (over 98%) whereas Ile-Pro-Pro is not bound. In conclusion, Ile-Pro-[
14C]Pro is partly absorbed intact and the distribution of radioactivity in tissues may be related to the blood-pressure-lowering effect of the peptide.</abstract><cop>Oxford</cop><pub>Elsevier Ltd</pub><doi>10.1016/j.idairyj.2007.02.004</doi><tpages>8</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0958-6946 |
| ispartof | International dairy journal, 2007-10, Vol.17 (10), p.1216-1223 |
| issn | 0958-6946 1879-0143 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_20469067 |
| source | Elsevier ScienceDirect Journals |
| subjects | Absorption animal models antihypertensive agents bioactive properties Biological and medical sciences excretion Food industries Fundamental and applied biological sciences. Psychology human nutrition Ile-Pro-Pro Kinetics milk Milk and cheese industries. Ice creams Milk-derived peptides oral administration Radioactive label radiolabeling rats tissue distribution tripeptides |
| title | Oral absorption, tissue distribution and excretion of a radiolabelled analog of a milk-derived antihypertensive peptide, Ile-Pro-Pro, in rats |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-26T20%3A50%3A27IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Oral%20absorption,%20tissue%20distribution%20and%20excretion%20of%20a%20radiolabelled%20analog%20of%20a%20milk-derived%20antihypertensive%20peptide,%20Ile-Pro-Pro,%20in%20rats&rft.jtitle=International%20dairy%20journal&rft.au=Jauhiainen,%20Tiina&rft.date=2007-10-01&rft.volume=17&rft.issue=10&rft.spage=1216&rft.epage=1223&rft.pages=1216-1223&rft.issn=0958-6946&rft.eissn=1879-0143&rft_id=info:doi/10.1016/j.idairyj.2007.02.004&rft_dat=%3Cproquest_cross%3E20469067%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=20469067&rft_id=info:pmid/&rft_els_id=S0958694607000404&rfr_iscdi=true |