The protein kinase ImeB is required for light‐mediated inhibition of sexual development and for mycotoxin production in Aspergillus nidulans
Summary Spore formation is a common process in the developmental cycle of fungi. In the yeast Saccharomyces cerevisiae, Ime2 is a key protein kinase for the meiotic cell cycle, which precedes ascospore formation. Here, we analysed the IME2‐related imeB gene of the filamentous ascomycete Aspergillus...
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| Veröffentlicht in: | Molecular microbiology 2009-03, Vol.71 (5), p.1278-1295 |
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| creator | Bayram, Özgür Sari, Fatih Braus, Gerhard H. Irniger, Stefan |
| description | Summary
Spore formation is a common process in the developmental cycle of fungi. In the yeast Saccharomyces cerevisiae, Ime2 is a key protein kinase for the meiotic cell cycle, which precedes ascospore formation. Here, we analysed the IME2‐related imeB gene of the filamentous ascomycete Aspergillus nidulans. imeB deletion strains are retarded in growth and overproduce fertile sexual fruiting bodies in the presence of light, which normally represses sexual development. imeB mutants also display abnormal differentiation of sexual Hülle cells in submerged cultures. Increased sexual development of imeB mutants is dependent on VeA, a component of the heterotrimeric velvet complex. A combined deletion of imeB with the phytochrome fphA, a red light receptor, results in a complete loss of light response, suggesting that ImeB and FphA cooperate in light‐mediated inhibition of sexual development. Furthermore, we found that imeB mutants fail to produce the mycotoxin sterigmatocystin, an aflatoxin precursor, and show that ImeB is needed for expression of the sterigmatocystin gene cluster. ImeB contains a TXY motif conserved in mitogen‐activated protein kinases. This sequence element is essential for ImeB function. We conclude that ImeB is a mitogen‐activated protein kinase‐related protein kinase required for the co‐ordinated control of light‐dependent development with mycotoxin production. |
| doi_str_mv | 10.1111/j.1365-2958.2009.06606.x |
| format | Article |
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Spore formation is a common process in the developmental cycle of fungi. In the yeast Saccharomyces cerevisiae, Ime2 is a key protein kinase for the meiotic cell cycle, which precedes ascospore formation. Here, we analysed the IME2‐related imeB gene of the filamentous ascomycete Aspergillus nidulans. imeB deletion strains are retarded in growth and overproduce fertile sexual fruiting bodies in the presence of light, which normally represses sexual development. imeB mutants also display abnormal differentiation of sexual Hülle cells in submerged cultures. Increased sexual development of imeB mutants is dependent on VeA, a component of the heterotrimeric velvet complex. A combined deletion of imeB with the phytochrome fphA, a red light receptor, results in a complete loss of light response, suggesting that ImeB and FphA cooperate in light‐mediated inhibition of sexual development. Furthermore, we found that imeB mutants fail to produce the mycotoxin sterigmatocystin, an aflatoxin precursor, and show that ImeB is needed for expression of the sterigmatocystin gene cluster. ImeB contains a TXY motif conserved in mitogen‐activated protein kinases. This sequence element is essential for ImeB function. We conclude that ImeB is a mitogen‐activated protein kinase‐related protein kinase required for the co‐ordinated control of light‐dependent development with mycotoxin production.</description><identifier>ISSN: 0950-382X</identifier><identifier>EISSN: 1365-2958</identifier><identifier>DOI: 10.1111/j.1365-2958.2009.06606.x</identifier><identifier>PMID: 19210625</identifier><language>eng</language><publisher>Oxford, UK: Blackwell Publishing Ltd</publisher><subject>Amino Acid Sequence ; Ascomycetes ; Aspergillus nidulans ; Aspergillus nidulans - genetics ; Aspergillus nidulans - growth & development ; Aspergillus nidulans - metabolism ; Biological and medical sciences ; Fundamental and applied biological sciences. Psychology ; Fungal Proteins - genetics ; Fungal Proteins - metabolism ; Gene Deletion ; Gene Expression Regulation, Developmental ; Gene Expression Regulation, Fungal ; Genes ; Genetic Complementation Test ; Kinases ; Light ; Microbiology ; Miscellaneous ; Molecular Sequence Data ; Multigene Family ; Mutation ; Mycology ; Mycotoxins - biosynthesis ; Protein Kinases - genetics ; Protein Kinases - metabolism ; RNA, Fungal - metabolism ; Saccharomyces cerevisiae ; Sequence Alignment ; Spores, Fungal - genetics ; Spores, Fungal - growth & development ; Spores, Fungal - metabolism ; Toxins ; Yeast</subject><ispartof>Molecular microbiology, 2009-03, Vol.71 (5), p.1278-1295</ispartof><rights>2009 The Authors. Journal compilation © 2009 Blackwell Publishing Ltd</rights><rights>2009 INIST-CNRS</rights><rights>Copyright Blackwell Publishing Ltd. Mar 2009</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c5216-50c43852970f4f2784b63a4fd0a5a88b5c7eba3c8c8f45fd39d91fb470b92d6f3</citedby><cites>FETCH-LOGICAL-c5216-50c43852970f4f2784b63a4fd0a5a88b5c7eba3c8c8f45fd39d91fb470b92d6f3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fj.1365-2958.2009.06606.x$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fj.1365-2958.2009.06606.x$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1416,1432,27922,27923,45572,45573,46407,46831</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=21177138$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/19210625$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Bayram, Özgür</creatorcontrib><creatorcontrib>Sari, Fatih</creatorcontrib><creatorcontrib>Braus, Gerhard H.</creatorcontrib><creatorcontrib>Irniger, Stefan</creatorcontrib><title>The protein kinase ImeB is required for light‐mediated inhibition of sexual development and for mycotoxin production in Aspergillus nidulans</title><title>Molecular microbiology</title><addtitle>Mol Microbiol</addtitle><description>Summary
Spore formation is a common process in the developmental cycle of fungi. In the yeast Saccharomyces cerevisiae, Ime2 is a key protein kinase for the meiotic cell cycle, which precedes ascospore formation. Here, we analysed the IME2‐related imeB gene of the filamentous ascomycete Aspergillus nidulans. imeB deletion strains are retarded in growth and overproduce fertile sexual fruiting bodies in the presence of light, which normally represses sexual development. imeB mutants also display abnormal differentiation of sexual Hülle cells in submerged cultures. Increased sexual development of imeB mutants is dependent on VeA, a component of the heterotrimeric velvet complex. A combined deletion of imeB with the phytochrome fphA, a red light receptor, results in a complete loss of light response, suggesting that ImeB and FphA cooperate in light‐mediated inhibition of sexual development. Furthermore, we found that imeB mutants fail to produce the mycotoxin sterigmatocystin, an aflatoxin precursor, and show that ImeB is needed for expression of the sterigmatocystin gene cluster. ImeB contains a TXY motif conserved in mitogen‐activated protein kinases. This sequence element is essential for ImeB function. We conclude that ImeB is a mitogen‐activated protein kinase‐related protein kinase required for the co‐ordinated control of light‐dependent development with mycotoxin production.</description><subject>Amino Acid Sequence</subject><subject>Ascomycetes</subject><subject>Aspergillus nidulans</subject><subject>Aspergillus nidulans - genetics</subject><subject>Aspergillus nidulans - growth & development</subject><subject>Aspergillus nidulans - metabolism</subject><subject>Biological and medical sciences</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Fungal Proteins - genetics</subject><subject>Fungal Proteins - metabolism</subject><subject>Gene Deletion</subject><subject>Gene Expression Regulation, Developmental</subject><subject>Gene Expression Regulation, Fungal</subject><subject>Genes</subject><subject>Genetic Complementation Test</subject><subject>Kinases</subject><subject>Light</subject><subject>Microbiology</subject><subject>Miscellaneous</subject><subject>Molecular Sequence Data</subject><subject>Multigene Family</subject><subject>Mutation</subject><subject>Mycology</subject><subject>Mycotoxins - biosynthesis</subject><subject>Protein Kinases - genetics</subject><subject>Protein Kinases - metabolism</subject><subject>RNA, Fungal - metabolism</subject><subject>Saccharomyces cerevisiae</subject><subject>Sequence Alignment</subject><subject>Spores, Fungal - genetics</subject><subject>Spores, Fungal - growth & development</subject><subject>Spores, Fungal - metabolism</subject><subject>Toxins</subject><subject>Yeast</subject><issn>0950-382X</issn><issn>1365-2958</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2009</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkU9vFCEYxonR2G31KxhiordZ-TMwzMFDbaxu0sZLTbwRhoEuKwNbmNHdm5_A-Bn9JDLdTU08yQVe-D0vT94HAIjREpf1ZrPElLOKtEwsCULtEnGO-HL3CCweHh6DBWoZqqggX07Aac4bhDBFnD4FJ7glGHHCFuDnzdrAbYqjcQF-dUFlA1eDeQddhsncTS6ZHtqYoHe36_H3j1-D6Z0ay6ULa9e50cUAo4XZ7CblYW--GR-3gwkjVOGgHPY6jnFX-pd_-knfS0p1nrcm3TrvpwyD6yevQn4Gnljls3l-3M_A58v3Nxcfq6tPH1YX51eVZgTziiFdU8FI2yBbW9KIuuNU1bZHiikhOqYb0ymqhRa2Zranbd9i29UN6lrSc0vPwOtD32LpbjJ5lIPL2vjiwcQpS4JqjpCoC_jyH3ATpxSKN4lbzlAZOy2QOEA6xZyTsXKb3KDSXmIk58DkRs65yDkXOQcm7wOTuyJ9cew_dWW0f4XHhArw6giorJW3SQXt8gNHMG4aTEXh3h64786b_X8bkNfXq_lE_wDvy7TE</recordid><startdate>200903</startdate><enddate>200903</enddate><creator>Bayram, Özgür</creator><creator>Sari, Fatih</creator><creator>Braus, Gerhard H.</creator><creator>Irniger, Stefan</creator><general>Blackwell Publishing Ltd</general><general>Blackwell</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7QP</scope><scope>7QR</scope><scope>7TK</scope><scope>7TM</scope><scope>7U9</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>H94</scope><scope>M7N</scope><scope>P64</scope><scope>RC3</scope><scope>7T7</scope><scope>7U7</scope></search><sort><creationdate>200903</creationdate><title>The protein kinase ImeB is required for light‐mediated inhibition of sexual development and for mycotoxin production in Aspergillus nidulans</title><author>Bayram, Özgür ; Sari, Fatih ; Braus, Gerhard H. ; Irniger, Stefan</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c5216-50c43852970f4f2784b63a4fd0a5a88b5c7eba3c8c8f45fd39d91fb470b92d6f3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2009</creationdate><topic>Amino Acid Sequence</topic><topic>Ascomycetes</topic><topic>Aspergillus nidulans</topic><topic>Aspergillus nidulans - genetics</topic><topic>Aspergillus nidulans - growth & development</topic><topic>Aspergillus nidulans - metabolism</topic><topic>Biological and medical sciences</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Fungal Proteins - genetics</topic><topic>Fungal Proteins - metabolism</topic><topic>Gene Deletion</topic><topic>Gene Expression Regulation, Developmental</topic><topic>Gene Expression Regulation, Fungal</topic><topic>Genes</topic><topic>Genetic Complementation Test</topic><topic>Kinases</topic><topic>Light</topic><topic>Microbiology</topic><topic>Miscellaneous</topic><topic>Molecular Sequence Data</topic><topic>Multigene Family</topic><topic>Mutation</topic><topic>Mycology</topic><topic>Mycotoxins - biosynthesis</topic><topic>Protein Kinases - genetics</topic><topic>Protein Kinases - metabolism</topic><topic>RNA, Fungal - metabolism</topic><topic>Saccharomyces cerevisiae</topic><topic>Sequence Alignment</topic><topic>Spores, Fungal - genetics</topic><topic>Spores, Fungal - growth & development</topic><topic>Spores, Fungal - metabolism</topic><topic>Toxins</topic><topic>Yeast</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Bayram, Özgür</creatorcontrib><creatorcontrib>Sari, Fatih</creatorcontrib><creatorcontrib>Braus, Gerhard H.</creatorcontrib><creatorcontrib>Irniger, Stefan</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Chemoreception Abstracts</collection><collection>Neurosciences Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Toxicology Abstracts</collection><jtitle>Molecular microbiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Bayram, Özgür</au><au>Sari, Fatih</au><au>Braus, Gerhard H.</au><au>Irniger, Stefan</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The protein kinase ImeB is required for light‐mediated inhibition of sexual development and for mycotoxin production in Aspergillus nidulans</atitle><jtitle>Molecular microbiology</jtitle><addtitle>Mol Microbiol</addtitle><date>2009-03</date><risdate>2009</risdate><volume>71</volume><issue>5</issue><spage>1278</spage><epage>1295</epage><pages>1278-1295</pages><issn>0950-382X</issn><eissn>1365-2958</eissn><abstract>Summary
Spore formation is a common process in the developmental cycle of fungi. In the yeast Saccharomyces cerevisiae, Ime2 is a key protein kinase for the meiotic cell cycle, which precedes ascospore formation. Here, we analysed the IME2‐related imeB gene of the filamentous ascomycete Aspergillus nidulans. imeB deletion strains are retarded in growth and overproduce fertile sexual fruiting bodies in the presence of light, which normally represses sexual development. imeB mutants also display abnormal differentiation of sexual Hülle cells in submerged cultures. Increased sexual development of imeB mutants is dependent on VeA, a component of the heterotrimeric velvet complex. A combined deletion of imeB with the phytochrome fphA, a red light receptor, results in a complete loss of light response, suggesting that ImeB and FphA cooperate in light‐mediated inhibition of sexual development. Furthermore, we found that imeB mutants fail to produce the mycotoxin sterigmatocystin, an aflatoxin precursor, and show that ImeB is needed for expression of the sterigmatocystin gene cluster. ImeB contains a TXY motif conserved in mitogen‐activated protein kinases. This sequence element is essential for ImeB function. We conclude that ImeB is a mitogen‐activated protein kinase‐related protein kinase required for the co‐ordinated control of light‐dependent development with mycotoxin production.</abstract><cop>Oxford, UK</cop><pub>Blackwell Publishing Ltd</pub><pmid>19210625</pmid><doi>10.1111/j.1365-2958.2009.06606.x</doi><tpages>18</tpages></addata></record> |
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| subjects | Amino Acid Sequence Ascomycetes Aspergillus nidulans Aspergillus nidulans - genetics Aspergillus nidulans - growth & development Aspergillus nidulans - metabolism Biological and medical sciences Fundamental and applied biological sciences. Psychology Fungal Proteins - genetics Fungal Proteins - metabolism Gene Deletion Gene Expression Regulation, Developmental Gene Expression Regulation, Fungal Genes Genetic Complementation Test Kinases Light Microbiology Miscellaneous Molecular Sequence Data Multigene Family Mutation Mycology Mycotoxins - biosynthesis Protein Kinases - genetics Protein Kinases - metabolism RNA, Fungal - metabolism Saccharomyces cerevisiae Sequence Alignment Spores, Fungal - genetics Spores, Fungal - growth & development Spores, Fungal - metabolism Toxins Yeast |
| title | The protein kinase ImeB is required for light‐mediated inhibition of sexual development and for mycotoxin production in Aspergillus nidulans |
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