Rhizobium etli opt operon is required for symbiosis and stress resistance
Rhizobium etli is a Gram-negative root-colonizing soil bacterium capable of fixing nitrogen while living in symbiosis with its leguminous host Phaseolus vulgaris. A genome-wide screening for R. etli symbiotic mutants revealed a R. etli operon encoding an oligopeptide ABC-transporter (Opt), two redA...
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| Veröffentlicht in: | Environmental microbiology 2007-07, Vol.9 (7), p.1665-1674 |
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| creator | Vos, K Braeken, K Fauvart, M Ndayizeye, M Verhaert, J Zachurzok, S Lambrichts, I Michiels, J |
| description | Rhizobium etli is a Gram-negative root-colonizing soil bacterium capable of fixing nitrogen while living in symbiosis with its leguminous host Phaseolus vulgaris. A genome-wide screening for R. etli symbiotic mutants revealed a R. etli operon encoding an oligopeptide ABC-transporter (Opt), two redA homologous genes and one redB gene. Expression analysis showed this opt operon to be transcribed both under free-living and symbiotic conditions and expression levels were demonstrated to be growth-phase-dependent. Plants nodulated by R. etli opt mutants showed a reduced symbiotic nitrogen fixation activity (approximately 50% reduction). Growth experiments with opt mutants in the presence of oligopeptides as the sole nitrogen source confirmed the involvement of the opt genes in oligopeptide uptake. Further phenotypic analysis of the opt mutants revealed them to display an enhanced resistance to the oligopeptide antibiotic bacitracin, an increased susceptibility to the β-lactam antibiotic ampicillin and a decreased osmotolerance. In conclusion, our results demonstrate that the opt operon plays a crucial role during symbiosis and stress resistance. |
| doi_str_mv | 10.1111/j.1462-2920.2007.01284.x |
| format | Article |
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A genome-wide screening for R. etli symbiotic mutants revealed a R. etli operon encoding an oligopeptide ABC-transporter (Opt), two redA homologous genes and one redB gene. Expression analysis showed this opt operon to be transcribed both under free-living and symbiotic conditions and expression levels were demonstrated to be growth-phase-dependent. Plants nodulated by R. etli opt mutants showed a reduced symbiotic nitrogen fixation activity (approximately 50% reduction). Growth experiments with opt mutants in the presence of oligopeptides as the sole nitrogen source confirmed the involvement of the opt genes in oligopeptide uptake. Further phenotypic analysis of the opt mutants revealed them to display an enhanced resistance to the oligopeptide antibiotic bacitracin, an increased susceptibility to the β-lactam antibiotic ampicillin and a decreased osmotolerance. 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A genome-wide screening for R. etli symbiotic mutants revealed a R. etli operon encoding an oligopeptide ABC-transporter (Opt), two redA homologous genes and one redB gene. Expression analysis showed this opt operon to be transcribed both under free-living and symbiotic conditions and expression levels were demonstrated to be growth-phase-dependent. Plants nodulated by R. etli opt mutants showed a reduced symbiotic nitrogen fixation activity (approximately 50% reduction). Growth experiments with opt mutants in the presence of oligopeptides as the sole nitrogen source confirmed the involvement of the opt genes in oligopeptide uptake. Further phenotypic analysis of the opt mutants revealed them to display an enhanced resistance to the oligopeptide antibiotic bacitracin, an increased susceptibility to the β-lactam antibiotic ampicillin and a decreased osmotolerance. In conclusion, our results demonstrate that the opt operon plays a crucial role during symbiosis and stress resistance.</description><subject>Ampicillin - toxicity</subject><subject>ATP-Binding Cassette Transporters - genetics</subject><subject>ATP-Binding Cassette Transporters - metabolism</subject><subject>Bacitracin - toxicity</subject><subject>Base Sequence</subject><subject>DNA Primers - genetics</subject><subject>Gene Components</subject><subject>Gene Expression Regulation, Bacterial</subject><subject>Glucuronidase - metabolism</subject><subject>Microscopy, Electron, Transmission</subject><subject>Molecular Sequence Data</subject><subject>Nitrogen Fixation</subject><subject>Oligopeptides - metabolism</subject><subject>Operon - genetics</subject><subject>Operon - physiology</subject><subject>Phaseolus - microbiology</subject><subject>Phaseolus vulgaris</subject><subject>Reverse Transcriptase Polymerase Chain Reaction</subject><subject>Rhizobium etli</subject><subject>Rhizobium etli - drug effects</subject><subject>Rhizobium etli - genetics</subject><subject>Rhizobium etli - growth & development</subject><subject>Rhizobium etli - ultrastructure</subject><subject>Sequence Analysis, DNA</subject><subject>Symbiosis</subject><issn>1462-2912</issn><issn>1462-2920</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2007</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkUFv1DAQhS0EoqXwFyAnbgkex0mcAwdU2rJSKYhSehw5zgS8JOutnYhdfj0OWS1XLFl-Gr83Y31mLAGeQVxv1hnIUqSiFjwTnFcZB6FktnvETo8Xj48axAl7FsKac6jyij9lJ1AVpSy5OGWrLz_sb9fYaUho7G3itmPc5N0msSHx9DBZT23SOZ-E_dBYF2JZb9okjJ7C7IiFUW8MPWdPOt0HenE4z9jd5cXX8w_p9aer1fm769TEiTKtmxYMz2UHtTG8iY-QNVRglCy6htq8qJQqSwNa511OUoKqRC0LY0pSvFWQn7HXS9-tdw8ThREHGwz1vd6QmwIKLos6BxGNajEa70Lw1OHW20H7PQLHGSOucSaEMy2cMeJfjLiL0ZeHGVMzUPsveOAWDW8Xwy_b0_6_G-PFx9WsYj5d8hEe7Y557X9iGf-owPubK7z89vnm_f1tgSr6Xy3-TjvU370NeHcrOOSxuYIyij94k5ir</recordid><startdate>200707</startdate><enddate>200707</enddate><creator>Vos, K</creator><creator>Braeken, K</creator><creator>Fauvart, M</creator><creator>Ndayizeye, M</creator><creator>Verhaert, J</creator><creator>Zachurzok, S</creator><creator>Lambrichts, I</creator><creator>Michiels, J</creator><general>Oxford, UK : Blackwell Publishing Ltd</general><general>Blackwell Publishing Ltd</general><scope>FBQ</scope><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>C1K</scope></search><sort><creationdate>200707</creationdate><title>Rhizobium etli opt operon is required for symbiosis and stress resistance</title><author>Vos, K ; Braeken, K ; Fauvart, M ; Ndayizeye, M ; Verhaert, J ; Zachurzok, S ; Lambrichts, I ; Michiels, J</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4604-9bd1c034f19cc0b60249171c845fbed3578866c1aa3f3e441872945cc6e80d813</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2007</creationdate><topic>Ampicillin - toxicity</topic><topic>ATP-Binding Cassette Transporters - genetics</topic><topic>ATP-Binding Cassette Transporters - metabolism</topic><topic>Bacitracin - toxicity</topic><topic>Base Sequence</topic><topic>DNA Primers - genetics</topic><topic>Gene Components</topic><topic>Gene Expression Regulation, Bacterial</topic><topic>Glucuronidase - metabolism</topic><topic>Microscopy, Electron, Transmission</topic><topic>Molecular Sequence Data</topic><topic>Nitrogen Fixation</topic><topic>Oligopeptides - metabolism</topic><topic>Operon - genetics</topic><topic>Operon - physiology</topic><topic>Phaseolus - microbiology</topic><topic>Phaseolus vulgaris</topic><topic>Reverse Transcriptase Polymerase Chain Reaction</topic><topic>Rhizobium etli</topic><topic>Rhizobium etli - drug effects</topic><topic>Rhizobium etli - genetics</topic><topic>Rhizobium etli - growth & development</topic><topic>Rhizobium etli - ultrastructure</topic><topic>Sequence Analysis, DNA</topic><topic>Symbiosis</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Vos, K</creatorcontrib><creatorcontrib>Braeken, K</creatorcontrib><creatorcontrib>Fauvart, M</creatorcontrib><creatorcontrib>Ndayizeye, M</creatorcontrib><creatorcontrib>Verhaert, J</creatorcontrib><creatorcontrib>Zachurzok, S</creatorcontrib><creatorcontrib>Lambrichts, I</creatorcontrib><creatorcontrib>Michiels, J</creatorcontrib><collection>AGRIS</collection><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Environmental Sciences and Pollution Management</collection><jtitle>Environmental microbiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Vos, K</au><au>Braeken, K</au><au>Fauvart, M</au><au>Ndayizeye, M</au><au>Verhaert, J</au><au>Zachurzok, S</au><au>Lambrichts, I</au><au>Michiels, J</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Rhizobium etli opt operon is required for symbiosis and stress resistance</atitle><jtitle>Environmental microbiology</jtitle><addtitle>Environ Microbiol</addtitle><date>2007-07</date><risdate>2007</risdate><volume>9</volume><issue>7</issue><spage>1665</spage><epage>1674</epage><pages>1665-1674</pages><issn>1462-2912</issn><eissn>1462-2920</eissn><abstract>Rhizobium etli is a Gram-negative root-colonizing soil bacterium capable of fixing nitrogen while living in symbiosis with its leguminous host Phaseolus vulgaris. A genome-wide screening for R. etli symbiotic mutants revealed a R. etli operon encoding an oligopeptide ABC-transporter (Opt), two redA homologous genes and one redB gene. Expression analysis showed this opt operon to be transcribed both under free-living and symbiotic conditions and expression levels were demonstrated to be growth-phase-dependent. Plants nodulated by R. etli opt mutants showed a reduced symbiotic nitrogen fixation activity (approximately 50% reduction). Growth experiments with opt mutants in the presence of oligopeptides as the sole nitrogen source confirmed the involvement of the opt genes in oligopeptide uptake. Further phenotypic analysis of the opt mutants revealed them to display an enhanced resistance to the oligopeptide antibiotic bacitracin, an increased susceptibility to the β-lactam antibiotic ampicillin and a decreased osmotolerance. In conclusion, our results demonstrate that the opt operon plays a crucial role during symbiosis and stress resistance.</abstract><cop>Oxford, UK</cop><pub>Oxford, UK : Blackwell Publishing Ltd</pub><pmid>17564602</pmid><doi>10.1111/j.1462-2920.2007.01284.x</doi><tpages>10</tpages></addata></record> |
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| subjects | Ampicillin - toxicity ATP-Binding Cassette Transporters - genetics ATP-Binding Cassette Transporters - metabolism Bacitracin - toxicity Base Sequence DNA Primers - genetics Gene Components Gene Expression Regulation, Bacterial Glucuronidase - metabolism Microscopy, Electron, Transmission Molecular Sequence Data Nitrogen Fixation Oligopeptides - metabolism Operon - genetics Operon - physiology Phaseolus - microbiology Phaseolus vulgaris Reverse Transcriptase Polymerase Chain Reaction Rhizobium etli Rhizobium etli - drug effects Rhizobium etli - genetics Rhizobium etli - growth & development Rhizobium etli - ultrastructure Sequence Analysis, DNA Symbiosis |
| title | Rhizobium etli opt operon is required for symbiosis and stress resistance |
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