Protoplast Fusion and Gene Recombination in the Uncommon Actinomycete Planobispora rosea Producing GE2270
An efficient method for protoplast generation for the uncommon actinomycete Planobispora rosea , the producer of the thiazolylpeptide antibiotic GE2270, was developed using a combination of hen egg white lysozyme and Streptomyces globisporus mutanolysin. This method converted more than 70% of vegeta...
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| Veröffentlicht in: | Journal of antibiotics 2007-07, Vol.60 (7), p.447-454 |
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| creator | Beltrametti, Fabrizio Barucco, Daniele Rossi, Roberta Selva, Enrico Marinelli, Flavia |
| description | An efficient method for protoplast generation for the uncommon actinomycete
Planobispora rosea
, the producer of the thiazolylpeptide antibiotic GE2270, was developed using a combination of hen egg white lysozyme and
Streptomyces globisporus
mutanolysin. This method converted more than 70% of vegetative mycelium to protoplasts, which were then regenerated with 50% efficiency in an optimized medium. When
P. rosea
protoplasts were efficiently fused, recombination between different antibiotic (streptomycin and gentamicin) resistance markers originated sensitive strains (str
s
gen
s
) at frequencies as high as 18% and double resistant fusants (str
r
gen
r
) at frequencies as high as 29%. Double resistant fusants showed GE2270 productivity intermediate between the productivity of the parental strains. Protoplast generation and fusion in
P. rosea
makes whole genome shuffling feasible as an approach to be used alternately with classical random mutagenesis in industrial strain improvement programs. |
| doi_str_mv | 10.1038/ja.2007.57 |
| format | Article |
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Planobispora rosea
, the producer of the thiazolylpeptide antibiotic GE2270, was developed using a combination of hen egg white lysozyme and
Streptomyces globisporus
mutanolysin. This method converted more than 70% of vegetative mycelium to protoplasts, which were then regenerated with 50% efficiency in an optimized medium. When
P. rosea
protoplasts were efficiently fused, recombination between different antibiotic (streptomycin and gentamicin) resistance markers originated sensitive strains (str
s
gen
s
) at frequencies as high as 18% and double resistant fusants (str
r
gen
r
) at frequencies as high as 29%. Double resistant fusants showed GE2270 productivity intermediate between the productivity of the parental strains. Protoplast generation and fusion in
P. rosea
makes whole genome shuffling feasible as an approach to be used alternately with classical random mutagenesis in industrial strain improvement programs.</description><identifier>ISSN: 0021-8820</identifier><identifier>EISSN: 1881-1469</identifier><identifier>DOI: 10.1038/ja.2007.57</identifier><identifier>PMID: 17721003</identifier><language>eng</language><publisher>London: Nature Publishing Group UK</publisher><subject>Acne ; Actinomycetales - genetics ; Actinomycetales - metabolism ; Animals ; Anti-Bacterial Agents - biosynthesis ; Anti-Bacterial Agents - pharmacology ; Antibiotics ; Bacteriology ; Bioorganic Chemistry ; Cloning ; Efficiency ; Fermentation ; Genes ; Genetic engineering ; Genomes ; Humans ; Life Sciences ; Medicinal Chemistry ; Microbial Sensitivity Tests ; Microbiology ; Organic Chemistry ; original-article ; Peptides, Cyclic - chemical synthesis ; Peptides, Cyclic - pharmacology ; Protoplasts ; Recombination, Genetic ; Streptomyces globisporus ; Thiazoles - chemical synthesis ; Thiazoles - pharmacology</subject><ispartof>Journal of antibiotics, 2007-07, Vol.60 (7), p.447-454</ispartof><rights>Japan Antibiotics Research Association 2007</rights><rights>Copyright Nature Publishing Group Jul 2007</rights><rights>Japan Antibiotics Research Association 2007.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c473t-4717e0c279915f83f3862ec4e3d14c66c43716408056f8ba5a26cba1fb89fc863</citedby><cites>FETCH-LOGICAL-c473t-4717e0c279915f83f3862ec4e3d14c66c43716408056f8ba5a26cba1fb89fc863</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27922,27923</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/17721003$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Beltrametti, Fabrizio</creatorcontrib><creatorcontrib>Barucco, Daniele</creatorcontrib><creatorcontrib>Rossi, Roberta</creatorcontrib><creatorcontrib>Selva, Enrico</creatorcontrib><creatorcontrib>Marinelli, Flavia</creatorcontrib><title>Protoplast Fusion and Gene Recombination in the Uncommon Actinomycete Planobispora rosea Producing GE2270</title><title>Journal of antibiotics</title><addtitle>J Antibiot</addtitle><addtitle>J Antibiot (Tokyo)</addtitle><description>An efficient method for protoplast generation for the uncommon actinomycete
Planobispora rosea
, the producer of the thiazolylpeptide antibiotic GE2270, was developed using a combination of hen egg white lysozyme and
Streptomyces globisporus
mutanolysin. This method converted more than 70% of vegetative mycelium to protoplasts, which were then regenerated with 50% efficiency in an optimized medium. When
P. rosea
protoplasts were efficiently fused, recombination between different antibiotic (streptomycin and gentamicin) resistance markers originated sensitive strains (str
s
gen
s
) at frequencies as high as 18% and double resistant fusants (str
r
gen
r
) at frequencies as high as 29%. Double resistant fusants showed GE2270 productivity intermediate between the productivity of the parental strains. Protoplast generation and fusion in
P. rosea
makes whole genome shuffling feasible as an approach to be used alternately with classical random mutagenesis in industrial strain improvement programs.</description><subject>Acne</subject><subject>Actinomycetales - genetics</subject><subject>Actinomycetales - metabolism</subject><subject>Animals</subject><subject>Anti-Bacterial Agents - biosynthesis</subject><subject>Anti-Bacterial Agents - pharmacology</subject><subject>Antibiotics</subject><subject>Bacteriology</subject><subject>Bioorganic Chemistry</subject><subject>Cloning</subject><subject>Efficiency</subject><subject>Fermentation</subject><subject>Genes</subject><subject>Genetic engineering</subject><subject>Genomes</subject><subject>Humans</subject><subject>Life Sciences</subject><subject>Medicinal Chemistry</subject><subject>Microbial Sensitivity Tests</subject><subject>Microbiology</subject><subject>Organic Chemistry</subject><subject>original-article</subject><subject>Peptides, Cyclic - chemical synthesis</subject><subject>Peptides, Cyclic - pharmacology</subject><subject>Protoplasts</subject><subject>Recombination, Genetic</subject><subject>Streptomyces globisporus</subject><subject>Thiazoles - chemical synthesis</subject><subject>Thiazoles - pharmacology</subject><issn>0021-8820</issn><issn>1881-1469</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2007</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNp9kU1rGzEQhkVpaZy0l_6AIij00LLu6GMl7TGExC0EEkJzFlp5NpXxSq60e8i_r1wbUhrIaZiZh3c-XkI-MFgyEObbxi05gF62-hVZMGNYw6TqXpMFAGeNMRxOyGkpGwChhTZvyQnTmrOaLki4zWlKu60rE72aS0iRurimK4xI79CnsQ_RTftyiHT6hfQ-1uJY83M_hZjGR48T0tuti6kPZZeyozkVdLQKr2cf4gNdXXKu4R15M7htwffHeEbury5_Xnxvrm9WPy7OrxsvtZgaqZlG8Fx3HWsHIwZhFEcvUayZ9Ep5KTRTEgy0ajC9ax1Xvnds6E03eKPEGfl80N3l9HvGMtkxFI_buiGmuVgOsq1_4BX89B-4SXOOdTfLZSd4KxV0L1FMG22gg1ZX6suB8vX4knGwuxxGlx8tA7s3yW6c3Ztk_8Ifj5JzP-L6CT26UoGvB6DUVnzA_M_M53J_AHQpmMQ</recordid><startdate>20070701</startdate><enddate>20070701</enddate><creator>Beltrametti, Fabrizio</creator><creator>Barucco, Daniele</creator><creator>Rossi, Roberta</creator><creator>Selva, Enrico</creator><creator>Marinelli, Flavia</creator><general>Nature Publishing Group UK</general><general>Nature Publishing Group</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QL</scope><scope>7T5</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>88I</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M2P</scope><scope>M7P</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>Q9U</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope></search><sort><creationdate>20070701</creationdate><title>Protoplast Fusion and Gene Recombination in the Uncommon Actinomycete Planobispora rosea Producing GE2270</title><author>Beltrametti, Fabrizio ; Barucco, Daniele ; Rossi, Roberta ; Selva, Enrico ; Marinelli, Flavia</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c473t-4717e0c279915f83f3862ec4e3d14c66c43716408056f8ba5a26cba1fb89fc863</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2007</creationdate><topic>Acne</topic><topic>Actinomycetales - 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Planobispora rosea
, the producer of the thiazolylpeptide antibiotic GE2270, was developed using a combination of hen egg white lysozyme and
Streptomyces globisporus
mutanolysin. This method converted more than 70% of vegetative mycelium to protoplasts, which were then regenerated with 50% efficiency in an optimized medium. When
P. rosea
protoplasts were efficiently fused, recombination between different antibiotic (streptomycin and gentamicin) resistance markers originated sensitive strains (str
s
gen
s
) at frequencies as high as 18% and double resistant fusants (str
r
gen
r
) at frequencies as high as 29%. Double resistant fusants showed GE2270 productivity intermediate between the productivity of the parental strains. Protoplast generation and fusion in
P. rosea
makes whole genome shuffling feasible as an approach to be used alternately with classical random mutagenesis in industrial strain improvement programs.</abstract><cop>London</cop><pub>Nature Publishing Group UK</pub><pmid>17721003</pmid><doi>10.1038/ja.2007.57</doi><tpages>8</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0021-8820 |
| ispartof | Journal of antibiotics, 2007-07, Vol.60 (7), p.447-454 |
| issn | 0021-8820 1881-1469 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_20453732 |
| source | MEDLINE; EZB-FREE-00999 freely available EZB journals |
| subjects | Acne Actinomycetales - genetics Actinomycetales - metabolism Animals Anti-Bacterial Agents - biosynthesis Anti-Bacterial Agents - pharmacology Antibiotics Bacteriology Bioorganic Chemistry Cloning Efficiency Fermentation Genes Genetic engineering Genomes Humans Life Sciences Medicinal Chemistry Microbial Sensitivity Tests Microbiology Organic Chemistry original-article Peptides, Cyclic - chemical synthesis Peptides, Cyclic - pharmacology Protoplasts Recombination, Genetic Streptomyces globisporus Thiazoles - chemical synthesis Thiazoles - pharmacology |
| title | Protoplast Fusion and Gene Recombination in the Uncommon Actinomycete Planobispora rosea Producing GE2270 |
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