Composition of the Stabilizer and Conditions of Lyophilization for Preserving Infectious Activity of Influenza Virus
For stabilization of vaccine preparations, they are lyophilized. The composition of the protective medium is an important parameter affecting the quality of the vaccine after drying. In view of the risk of spreading prion diseases, the use of media containing animal proteins is not recommended. In t...
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| Veröffentlicht in: | Bulletin of experimental biology and medicine 2018-05, Vol.165 (1), p.52-56 |
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| creator | Dubrovina, I. A. Kiseleva, I. V. Kireeva, E. V. Rudenko, L. G. |
| description | For stabilization of vaccine preparations, they are lyophilized. The composition of the protective medium is an important parameter affecting the quality of the vaccine after drying. In view of the risk of spreading prion diseases, the use of media containing animal proteins is not recommended. In this study, protective media containing no animal proteins and lyophilization regimen were determined. The optimum lyophilization regimen consisted of three stages: freezing at -70°C, main stage at -35°C, and drying at 24°C. Protective medium containing 4% trehalose or protective medium with 10% sucrose and 5% soy peptone ensured highest stability of the lyophilized vaccine preparation in temperature range of 4-24°C. This can help to overcome possible break in the cold chain, which is important during transporting or storage of vaccine preparations. |
| doi_str_mv | 10.1007/s10517-018-4097-7 |
| format | Article |
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Protective medium containing 4% trehalose or protective medium with 10% sucrose and 5% soy peptone ensured highest stability of the lyophilized vaccine preparation in temperature range of 4-24°C. 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| subjects | Animals Biomedical and Life Sciences Biomedicine Cell Biology Disease transmission Dogs Drug Stability Freeze Drying - methods Influenza Influenza Vaccines Influenza viruses Internal Medicine Laboratory Medicine Madin Darby Canine Kidney Cells Orthomyxoviridae - drug effects Orthomyxoviridae - immunology Pathology Peptones - pharmacology Sucrose - pharmacology Virology |
| title | Composition of the Stabilizer and Conditions of Lyophilization for Preserving Infectious Activity of Influenza Virus |
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