CRISPR‐Based Targeted Epigenetic Editing Enables Gene Expression Modulation of the Silenced Beta‐Galactoside Alpha‐2,6‐Sialyltransferase 1 in CHO Cells

Despite great efforts to control and modify gene expression of Chinese Hamster Ovary (CHO) cells by conventional genetic engineering approaches, i.e. overexpression or knockdown/‐out, subclonal variation, induced unknown regulatory effects as well as overexpression stress are still a major hurdle fo...

Ausführliche Beschreibung

Gespeichert in:

Bibliographische Detailangaben
Veröffentlicht in:	Biotechnology journal 2018-10, Vol.13 (10), p.e1700217-n/a
Hauptverfasser:	Marx, Nicolas, Grünwald‐Gruber, Clemens, Bydlinski, Nina, Dhiman, Heena, Ngoc Nguyen, Ly, Klanert, Gerald, Borth, Nicole
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Biocatalysis CHO CHO Cells Clustered Regularly Interspaced Short Palindromic Repeats - genetics Cricetulus CRISPR DNA methylation DNA Modification Methylases - metabolism epigenetic editing Epigenomics - methods Escherichia coli Galactosides - genetics Gene Editing - methods Gene Expression Genetic Engineering - methods Genomics glycosylation Promoter Regions, Genetic - genetics Recombinant Proteins - genetics Sialyltransferases - genetics
Online-Zugang:	Volltext
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!

Schreiben Sie den ersten Kommentar!