Atrial natriuretic peptide accelerates human endothelial progenitor cell–stimulated cutaneous wound healing and angiogenesis
ABSTRACT Atrial natriuretic peptide (ANP) is a powerful vasodilating peptide secreted by cardiac muscle cells, and endothelial progenitor cells (EPCs) have been reported to stimulate cutaneous wound healing by mediating angiogenesis. To determine whether ANP can promote the EPC‐mediated repair of in...
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| Veröffentlicht in: | Wound repair and regeneration 2018-03, Vol.26 (2), p.116-126 |
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| container_title | Wound repair and regeneration |
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| creator | Lee, Tae Wook Kwon, Yang Woo Park, Gyu Tae Do, Eun Kyoung Yoon, Jung Won Kim, Seung‐Chul Ko, Hyun‐Chang Kim, Moon‐Bum Kim, Jae Ho |
| description | ABSTRACT
Atrial natriuretic peptide (ANP) is a powerful vasodilating peptide secreted by cardiac muscle cells, and endothelial progenitor cells (EPCs) have been reported to stimulate cutaneous wound healing by mediating angiogenesis. To determine whether ANP can promote the EPC‐mediated repair of injured tissues, we examined the effects of ANP on the angiogenic properties of EPCs and on cutaneous wound healing. In vitro, ANP treatment enhanced the migration, proliferation, and endothelial tube‐forming abilities of EPCs. Furthermore, small interfering RNA‐mediated silencing of natriuretic peptide receptor‐1, which is a receptor for ANP, abrogated ANP‐induced migration, tube formation, and proliferation of EPCs. In a murine cutaneous wound model, administration of either ANP or EPCs had no significant effect on cutaneous wound healing or angiogenesis in vivo, whereas the coadministration of ANP and EPCs synergistically potentiated wound healing and angiogenesis. In addition, ANP promoted the survival and incorporation of transplanted EPCs into newly formed blood vessels in wounds. These results suggest ANP accelerates EPC‐mediated cutaneous wound healing by promoting the angiogenic properties and survival of transplanted EPCs. |
| doi_str_mv | 10.1111/wrr.12641 |
| format | Article |
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Atrial natriuretic peptide (ANP) is a powerful vasodilating peptide secreted by cardiac muscle cells, and endothelial progenitor cells (EPCs) have been reported to stimulate cutaneous wound healing by mediating angiogenesis. To determine whether ANP can promote the EPC‐mediated repair of injured tissues, we examined the effects of ANP on the angiogenic properties of EPCs and on cutaneous wound healing. In vitro, ANP treatment enhanced the migration, proliferation, and endothelial tube‐forming abilities of EPCs. Furthermore, small interfering RNA‐mediated silencing of natriuretic peptide receptor‐1, which is a receptor for ANP, abrogated ANP‐induced migration, tube formation, and proliferation of EPCs. In a murine cutaneous wound model, administration of either ANP or EPCs had no significant effect on cutaneous wound healing or angiogenesis in vivo, whereas the coadministration of ANP and EPCs synergistically potentiated wound healing and angiogenesis. In addition, ANP promoted the survival and incorporation of transplanted EPCs into newly formed blood vessels in wounds. These results suggest ANP accelerates EPC‐mediated cutaneous wound healing by promoting the angiogenic properties and survival of transplanted EPCs.</description><identifier>ISSN: 1067-1927</identifier><identifier>EISSN: 1524-475X</identifier><identifier>DOI: 10.1111/wrr.12641</identifier><identifier>PMID: 29802745</identifier><language>eng</language><publisher>United States</publisher><subject>Animals ; Atrial Natriuretic Factor - pharmacology ; Cell Proliferation - physiology ; Cells, Cultured ; Disease Models, Animal ; Endothelial Progenitor Cells - drug effects ; Endothelial Progenitor Cells - physiology ; Humans ; Immunohistochemistry ; In Vitro Techniques ; Mice ; Mice, Inbred BALB C ; Neovascularization, Physiologic - drug effects ; Real-Time Polymerase Chain Reaction ; Wound Healing - drug effects ; Wounds and Injuries - pathology</subject><ispartof>Wound repair and regeneration, 2018-03, Vol.26 (2), p.116-126</ispartof><rights>2018 by the Wound Healing Society</rights><rights>2018 by the Wound Healing Society.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3251-189423cbc41c350f0b5c1076234be3b79ca46c9a142d01c05a93ff320b36ad3e3</citedby><cites>FETCH-LOGICAL-c3251-189423cbc41c350f0b5c1076234be3b79ca46c9a142d01c05a93ff320b36ad3e3</cites><orcidid>0000-0003-4323-4790</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fwrr.12641$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fwrr.12641$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1416,27923,27924,45573,45574</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/29802745$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Lee, Tae Wook</creatorcontrib><creatorcontrib>Kwon, Yang Woo</creatorcontrib><creatorcontrib>Park, Gyu Tae</creatorcontrib><creatorcontrib>Do, Eun Kyoung</creatorcontrib><creatorcontrib>Yoon, Jung Won</creatorcontrib><creatorcontrib>Kim, Seung‐Chul</creatorcontrib><creatorcontrib>Ko, Hyun‐Chang</creatorcontrib><creatorcontrib>Kim, Moon‐Bum</creatorcontrib><creatorcontrib>Kim, Jae Ho</creatorcontrib><title>Atrial natriuretic peptide accelerates human endothelial progenitor cell–stimulated cutaneous wound healing and angiogenesis</title><title>Wound repair and regeneration</title><addtitle>Wound Repair Regen</addtitle><description>ABSTRACT
Atrial natriuretic peptide (ANP) is a powerful vasodilating peptide secreted by cardiac muscle cells, and endothelial progenitor cells (EPCs) have been reported to stimulate cutaneous wound healing by mediating angiogenesis. To determine whether ANP can promote the EPC‐mediated repair of injured tissues, we examined the effects of ANP on the angiogenic properties of EPCs and on cutaneous wound healing. In vitro, ANP treatment enhanced the migration, proliferation, and endothelial tube‐forming abilities of EPCs. Furthermore, small interfering RNA‐mediated silencing of natriuretic peptide receptor‐1, which is a receptor for ANP, abrogated ANP‐induced migration, tube formation, and proliferation of EPCs. In a murine cutaneous wound model, administration of either ANP or EPCs had no significant effect on cutaneous wound healing or angiogenesis in vivo, whereas the coadministration of ANP and EPCs synergistically potentiated wound healing and angiogenesis. In addition, ANP promoted the survival and incorporation of transplanted EPCs into newly formed blood vessels in wounds. These results suggest ANP accelerates EPC‐mediated cutaneous wound healing by promoting the angiogenic properties and survival of transplanted EPCs.</description><subject>Animals</subject><subject>Atrial Natriuretic Factor - pharmacology</subject><subject>Cell Proliferation - physiology</subject><subject>Cells, Cultured</subject><subject>Disease Models, Animal</subject><subject>Endothelial Progenitor Cells - drug effects</subject><subject>Endothelial Progenitor Cells - physiology</subject><subject>Humans</subject><subject>Immunohistochemistry</subject><subject>In Vitro Techniques</subject><subject>Mice</subject><subject>Mice, Inbred BALB C</subject><subject>Neovascularization, Physiologic - drug effects</subject><subject>Real-Time Polymerase Chain Reaction</subject><subject>Wound Healing - drug effects</subject><subject>Wounds and Injuries - pathology</subject><issn>1067-1927</issn><issn>1524-475X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2018</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1kMtKw0AYhQdRbK0ufAGZpS7SzjVplkW8QUEQRXdhMvnTjuRSZyaUbsR38A19EqemuvPfnH_xncPhIHRKyZiGm6ytHVMWC7qHhlQyEYlEvuyHn8RJRFOWDNCRc6-EECnT6SEasHRKWCLkEL3PvDWqwo0K2lnwRuMVrLwpACutoQKrPDi87GrVYGiK1i-h2jpWtl1AY3xrccCqr49P503dVQEvsO68aqDtHF63XVPgJajKNAuswq-ahdlawRl3jA5KVTk42ekIPV1fPV7eRvP7m7vL2TzSnEka0WkqGNe5FlRzSUqSS01JEjMucuB5kmolYp0qKlhBqCZSpbwsOSM5j1XBgY_QeZ8bWr914HxWG7et3bfMGBGSJVRORUAvelTb1jkLZbayplZ2k1GSbefOwtzZz9yBPdvFdnkNxR_5u28AJj2wNhVs_k_Knh8e-shvvCWN9A</recordid><startdate>201803</startdate><enddate>201803</enddate><creator>Lee, Tae Wook</creator><creator>Kwon, Yang Woo</creator><creator>Park, Gyu Tae</creator><creator>Do, Eun Kyoung</creator><creator>Yoon, Jung Won</creator><creator>Kim, Seung‐Chul</creator><creator>Ko, Hyun‐Chang</creator><creator>Kim, Moon‐Bum</creator><creator>Kim, Jae Ho</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0003-4323-4790</orcidid></search><sort><creationdate>201803</creationdate><title>Atrial natriuretic peptide accelerates human endothelial progenitor cell–stimulated cutaneous wound healing and angiogenesis</title><author>Lee, Tae Wook ; Kwon, Yang Woo ; Park, Gyu Tae ; Do, Eun Kyoung ; Yoon, Jung Won ; Kim, Seung‐Chul ; Ko, Hyun‐Chang ; Kim, Moon‐Bum ; Kim, Jae Ho</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3251-189423cbc41c350f0b5c1076234be3b79ca46c9a142d01c05a93ff320b36ad3e3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2018</creationdate><topic>Animals</topic><topic>Atrial Natriuretic Factor - pharmacology</topic><topic>Cell Proliferation - physiology</topic><topic>Cells, Cultured</topic><topic>Disease Models, Animal</topic><topic>Endothelial Progenitor Cells - drug effects</topic><topic>Endothelial Progenitor Cells - physiology</topic><topic>Humans</topic><topic>Immunohistochemistry</topic><topic>In Vitro Techniques</topic><topic>Mice</topic><topic>Mice, Inbred BALB C</topic><topic>Neovascularization, Physiologic - drug effects</topic><topic>Real-Time Polymerase Chain Reaction</topic><topic>Wound Healing - drug effects</topic><topic>Wounds and Injuries - pathology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Lee, Tae Wook</creatorcontrib><creatorcontrib>Kwon, Yang Woo</creatorcontrib><creatorcontrib>Park, Gyu Tae</creatorcontrib><creatorcontrib>Do, Eun Kyoung</creatorcontrib><creatorcontrib>Yoon, Jung Won</creatorcontrib><creatorcontrib>Kim, Seung‐Chul</creatorcontrib><creatorcontrib>Ko, Hyun‐Chang</creatorcontrib><creatorcontrib>Kim, Moon‐Bum</creatorcontrib><creatorcontrib>Kim, Jae Ho</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Wound repair and regeneration</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Lee, Tae Wook</au><au>Kwon, Yang Woo</au><au>Park, Gyu Tae</au><au>Do, Eun Kyoung</au><au>Yoon, Jung Won</au><au>Kim, Seung‐Chul</au><au>Ko, Hyun‐Chang</au><au>Kim, Moon‐Bum</au><au>Kim, Jae Ho</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Atrial natriuretic peptide accelerates human endothelial progenitor cell–stimulated cutaneous wound healing and angiogenesis</atitle><jtitle>Wound repair and regeneration</jtitle><addtitle>Wound Repair Regen</addtitle><date>2018-03</date><risdate>2018</risdate><volume>26</volume><issue>2</issue><spage>116</spage><epage>126</epage><pages>116-126</pages><issn>1067-1927</issn><eissn>1524-475X</eissn><abstract>ABSTRACT
Atrial natriuretic peptide (ANP) is a powerful vasodilating peptide secreted by cardiac muscle cells, and endothelial progenitor cells (EPCs) have been reported to stimulate cutaneous wound healing by mediating angiogenesis. To determine whether ANP can promote the EPC‐mediated repair of injured tissues, we examined the effects of ANP on the angiogenic properties of EPCs and on cutaneous wound healing. In vitro, ANP treatment enhanced the migration, proliferation, and endothelial tube‐forming abilities of EPCs. Furthermore, small interfering RNA‐mediated silencing of natriuretic peptide receptor‐1, which is a receptor for ANP, abrogated ANP‐induced migration, tube formation, and proliferation of EPCs. In a murine cutaneous wound model, administration of either ANP or EPCs had no significant effect on cutaneous wound healing or angiogenesis in vivo, whereas the coadministration of ANP and EPCs synergistically potentiated wound healing and angiogenesis. In addition, ANP promoted the survival and incorporation of transplanted EPCs into newly formed blood vessels in wounds. These results suggest ANP accelerates EPC‐mediated cutaneous wound healing by promoting the angiogenic properties and survival of transplanted EPCs.</abstract><cop>United States</cop><pmid>29802745</pmid><doi>10.1111/wrr.12641</doi><tpages>11</tpages><orcidid>https://orcid.org/0000-0003-4323-4790</orcidid></addata></record> |
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| subjects | Animals Atrial Natriuretic Factor - pharmacology Cell Proliferation - physiology Cells, Cultured Disease Models, Animal Endothelial Progenitor Cells - drug effects Endothelial Progenitor Cells - physiology Humans Immunohistochemistry In Vitro Techniques Mice Mice, Inbred BALB C Neovascularization, Physiologic - drug effects Real-Time Polymerase Chain Reaction Wound Healing - drug effects Wounds and Injuries - pathology |
| title | Atrial natriuretic peptide accelerates human endothelial progenitor cell–stimulated cutaneous wound healing and angiogenesis |
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