Effect of different electrical stimulation waves on orientation and alignment of adipose derived mesenchymal stem cells
To investigate the effect of different electrical stimulation waves on orientation and alignment of adipose derived mesenchymal stem cells (ADSCs). ADSCs were isolated from 5-week-old Sprague Dawley rats (weight, 100-150 g) and cultivated. The cells at passages 3-5 were inoculated to prepare cell cl...
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| Veröffentlicht in: | Zhongguo xiu fu chong jian wai ke za zhi 2017-07, Vol.31 (7), p.853 |
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| container_title | Zhongguo xiu fu chong jian wai ke za zhi |
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| creator | Long, Haiyan Yang, Gang Ma, Kunlong Xiao, Zhenghua Ren, Xiaomei |
| description | To investigate the effect of different electrical stimulation waves on orientation and alignment of adipose derived mesenchymal stem cells (ADSCs).
ADSCs were isolated from 5-week-old Sprague Dawley rats (weight, 100-150 g) and cultivated. The cells at passages 3-5 were inoculated to prepare cell climbing slices, subsequently was exposed to direct-current electrical stimulations (ES) at electric field strengths of 1, 2, 3, 4, 5, and 6 V/cm on a homemade electric field bioreactor (groups A1, A2, A3, A4, A5, and A6); at electric field strength of 6 V/cm, at 50% duty cycle, and at frequency of 1 and 2 Hz (groups B1 and B2) of square wave ES; at electric field strength of 6 V/cm, at pulse width of 2 ms, and at frequency of 1 and 2 Hz (groups C1 and C2) of biphasic pulse wave ES; and no ES was given as a control (group D). The changes of cellular morphology affected by applied ES were evaluated by time-lapse micropho-tography via inverted microscope. The cell alignment was evaluated via average orientation factor |
| doi_str_mv | 10.7507/1002-1892.201702027 |
| format | Article |
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ADSCs were isolated from 5-week-old Sprague Dawley rats (weight, 100-150 g) and cultivated. The cells at passages 3-5 were inoculated to prepare cell climbing slices, subsequently was exposed to direct-current electrical stimulations (ES) at electric field strengths of 1, 2, 3, 4, 5, and 6 V/cm on a homemade electric field bioreactor (groups A1, A2, A3, A4, A5, and A6); at electric field strength of 6 V/cm, at 50% duty cycle, and at frequency of 1 and 2 Hz (groups B1 and B2) of square wave ES; at electric field strength of 6 V/cm, at pulse width of 2 ms, and at frequency of 1 and 2 Hz (groups C1 and C2) of biphasic pulse wave ES; and no ES was given as a control (group D). The changes of cellular morphology affected by applied ES were evaluated by time-lapse micropho-tography via inverted microscope. The cell alignment was evaluated via average orientation factor</description><identifier>ISSN: 1002-1892</identifier><identifier>DOI: 10.7507/1002-1892.201702027</identifier><identifier>PMID: 29798532</identifier><language>chi</language><publisher>China</publisher><subject>Adipose Tissue - cytology ; Adipose Tissue - physiology ; Animals ; Cells, Cultured ; Electric Stimulation ; Mesenchymal Stem Cells - physiology ; Mosquito Vectors ; Rats ; Rats, Sprague-Dawley</subject><ispartof>Zhongguo xiu fu chong jian wai ke za zhi, 2017-07, Vol.31 (7), p.853</ispartof><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27904,27905</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/29798532$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Long, Haiyan</creatorcontrib><creatorcontrib>Yang, Gang</creatorcontrib><creatorcontrib>Ma, Kunlong</creatorcontrib><creatorcontrib>Xiao, Zhenghua</creatorcontrib><creatorcontrib>Ren, Xiaomei</creatorcontrib><title>Effect of different electrical stimulation waves on orientation and alignment of adipose derived mesenchymal stem cells</title><title>Zhongguo xiu fu chong jian wai ke za zhi</title><addtitle>Zhongguo Xiu Fu Chong Jian Wai Ke Za Zhi</addtitle><description>To investigate the effect of different electrical stimulation waves on orientation and alignment of adipose derived mesenchymal stem cells (ADSCs).
ADSCs were isolated from 5-week-old Sprague Dawley rats (weight, 100-150 g) and cultivated. The cells at passages 3-5 were inoculated to prepare cell climbing slices, subsequently was exposed to direct-current electrical stimulations (ES) at electric field strengths of 1, 2, 3, 4, 5, and 6 V/cm on a homemade electric field bioreactor (groups A1, A2, A3, A4, A5, and A6); at electric field strength of 6 V/cm, at 50% duty cycle, and at frequency of 1 and 2 Hz (groups B1 and B2) of square wave ES; at electric field strength of 6 V/cm, at pulse width of 2 ms, and at frequency of 1 and 2 Hz (groups C1 and C2) of biphasic pulse wave ES; and no ES was given as a control (group D). The changes of cellular morphology affected by applied ES were evaluated by time-lapse micropho-tography via inverted microscope. The cell alignment was evaluated via average orientation factor</description><subject>Adipose Tissue - cytology</subject><subject>Adipose Tissue - physiology</subject><subject>Animals</subject><subject>Cells, Cultured</subject><subject>Electric Stimulation</subject><subject>Mesenchymal Stem Cells - physiology</subject><subject>Mosquito Vectors</subject><subject>Rats</subject><subject>Rats, Sprague-Dawley</subject><issn>1002-1892</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2017</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNo9kLFOwzAQhj2AaFX6BEjII0vK2Ylje0RVoUiVWGCOHPsCluIkxEmrvj0pLUz369N_n05HyB2DlRQgHxkAT5jSfMWBSeDA5RWZ_9MZWcboSxAszVUm0hsy41pqJVI-J4dNVaEdaFtR56fYYzNQrCfUe2tqGgcfxtoMvm3owewx0im0vZ9qZ2gaR03tP5tw2pw0xvmujUgd9n6PjgaM2NivY_i1YaAW6zrekuvK1BGXl7kgH8-b9_U22b29vK6fdknHeD4kCmzpdAZOWpcjNygdF05V0mhkKgcuUqu1EXlWKYBScqV0CZlmVmJuuUkX5OHs7fr2e8Q4FMHH0wWmwXaMBYdM8FxpkFP1_lIdy4Cu6HofTH8s_p6V_gDJam1t</recordid><startdate>20170715</startdate><enddate>20170715</enddate><creator>Long, Haiyan</creator><creator>Yang, Gang</creator><creator>Ma, Kunlong</creator><creator>Xiao, Zhenghua</creator><creator>Ren, Xiaomei</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7X8</scope></search><sort><creationdate>20170715</creationdate><title>Effect of different electrical stimulation waves on orientation and alignment of adipose derived mesenchymal stem cells</title><author>Long, Haiyan ; Yang, Gang ; Ma, Kunlong ; Xiao, Zhenghua ; Ren, Xiaomei</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-p126t-80cbd940d7cd6e2ae7d25d8f7a9e1860253c99a564f800b72889b0491c7e6c2a3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>chi</language><creationdate>2017</creationdate><topic>Adipose Tissue - cytology</topic><topic>Adipose Tissue - physiology</topic><topic>Animals</topic><topic>Cells, Cultured</topic><topic>Electric Stimulation</topic><topic>Mesenchymal Stem Cells - physiology</topic><topic>Mosquito Vectors</topic><topic>Rats</topic><topic>Rats, Sprague-Dawley</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Long, Haiyan</creatorcontrib><creatorcontrib>Yang, Gang</creatorcontrib><creatorcontrib>Ma, Kunlong</creatorcontrib><creatorcontrib>Xiao, Zhenghua</creatorcontrib><creatorcontrib>Ren, Xiaomei</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>MEDLINE - Academic</collection><jtitle>Zhongguo xiu fu chong jian wai ke za zhi</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Long, Haiyan</au><au>Yang, Gang</au><au>Ma, Kunlong</au><au>Xiao, Zhenghua</au><au>Ren, Xiaomei</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Effect of different electrical stimulation waves on orientation and alignment of adipose derived mesenchymal stem cells</atitle><jtitle>Zhongguo xiu fu chong jian wai ke za zhi</jtitle><addtitle>Zhongguo Xiu Fu Chong Jian Wai Ke Za Zhi</addtitle><date>2017-07-15</date><risdate>2017</risdate><volume>31</volume><issue>7</issue><spage>853</spage><pages>853-</pages><issn>1002-1892</issn><abstract>To investigate the effect of different electrical stimulation waves on orientation and alignment of adipose derived mesenchymal stem cells (ADSCs).
ADSCs were isolated from 5-week-old Sprague Dawley rats (weight, 100-150 g) and cultivated. The cells at passages 3-5 were inoculated to prepare cell climbing slices, subsequently was exposed to direct-current electrical stimulations (ES) at electric field strengths of 1, 2, 3, 4, 5, and 6 V/cm on a homemade electric field bioreactor (groups A1, A2, A3, A4, A5, and A6); at electric field strength of 6 V/cm, at 50% duty cycle, and at frequency of 1 and 2 Hz (groups B1 and B2) of square wave ES; at electric field strength of 6 V/cm, at pulse width of 2 ms, and at frequency of 1 and 2 Hz (groups C1 and C2) of biphasic pulse wave ES; and no ES was given as a control (group D). The changes of cellular morphology affected by applied ES were evaluated by time-lapse micropho-tography via inverted microscope. The cell alignment was evaluated via average orientation factor</abstract><cop>China</cop><pmid>29798532</pmid><doi>10.7507/1002-1892.201702027</doi></addata></record> |
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| ispartof | Zhongguo xiu fu chong jian wai ke za zhi, 2017-07, Vol.31 (7), p.853 |
| issn | 1002-1892 |
| language | chi |
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| source | MEDLINE; EZB-FREE-00999 freely available EZB journals; PubMed Central |
| subjects | Adipose Tissue - cytology Adipose Tissue - physiology Animals Cells, Cultured Electric Stimulation Mesenchymal Stem Cells - physiology Mosquito Vectors Rats Rats, Sprague-Dawley |
| title | Effect of different electrical stimulation waves on orientation and alignment of adipose derived mesenchymal stem cells |
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