Evidence for the participation of  -hexosaminidase in human sperm-zona pellucida interaction in vitro

Evidence for the participation of  -hexosaminidase in human sperm-zona pellucida interaction in vitro

Mammalian sperm-zona pellucida (ZP) interaction is mediated by sperm lectin-like proteins and ZP glycoproteins. We have previously reported the participation of binding sites for N-acetylglucosamine (GlcNAc) residues in human sperm function, including sperm interaction with the ZP. Additionally, pre...

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Veröffentlicht in:Molecular human reproduction 2000-08, Vol.6 (8), p.699-706
Hauptverfasser: Miranda, Patricia V, Gonzalez-Echeverria, Fernanda, Blaquier, Jorge A, Mahuran, Don J, Tezon, Jorge G
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container_end_page 706
container_issue 8
container_start_page 699
container_title Molecular human reproduction
container_volume 6
creator Miranda, Patricia V
Gonzalez-Echeverria, Fernanda
Blaquier, Jorge A
Mahuran, Don J
Tezon, Jorge G
description Mammalian sperm-zona pellucida (ZP) interaction is mediated by sperm lectin-like proteins and ZP glycoproteins. We have previously reported the participation of binding sites for N-acetylglucosamine (GlcNAc) residues in human sperm function, including sperm interaction with the ZP. Additionally, previous results from our laboratory suggested that some of these events may be mediated by the glycosidase N-acetylglucosaminidase ([beta]-hexosaminidase, Hex, in mammals). In this study, we report the possible participation of Hex in human sperm-ZP interaction. Human recombinant Hex (hrHex) was obtained by expression in a stable transfected CHO cell line. When the recombinant enzyme was present during hemizona (HZ) assays, the number of sperm bound per HZ was significantly reduced. The same result was obtained when HZ were preincubated with hrHex. Additionally, the presence of a Hex-specific substrate during the HZ assay produced the same inhibitory effect. These results suggest the participation of a sperm Hex in the interaction with human ZP in vitro.
doi_str_mv 10.1093/molehr/6.8.699
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title Evidence for the participation of  -hexosaminidase in human sperm-zona pellucida interaction in vitro
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