Evaluation of Seed Storage Conditions and Genetic Diversity of Four Crucifers Endemic to Spain

Traditional assays (germination and vigour), as well as biochemical assays using isozyme loci, were performed to evaluate the effect of different seed storage conditions in Coincya rupestris, Iberis pectinata, Moricandia moricandiodes and Vella pseudocytisus. Seeds had been stored for 24-30 years un...

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Veröffentlicht in:Annals of botany 1999-08, Vol.84 (2), p.207-212
Hauptverfasser: MASELLI, S., PÉREZ-GARCÍA, F., AGUINAGALDE, I.
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container_title Annals of botany
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creator MASELLI, S.
PÉREZ-GARCÍA, F.
AGUINAGALDE, I.
description Traditional assays (germination and vigour), as well as biochemical assays using isozyme loci, were performed to evaluate the effect of different seed storage conditions in Coincya rupestris, Iberis pectinata, Moricandia moricandiodes and Vella pseudocytisus. Seeds had been stored for 24-30 years under long-term (LT) and short-term (ST) conditions. Fresh seeds of the same species from natural populations (NP) were also collected in 1996 and used for comparison. Isozyme loci were used as markers of genetic deterioration and to determine the genetic diversity of samples. The germinability, vigour and enzyme activity of LT samples were comparable to values obtained using freshly collected NP samples, showing the effectiveness of the long-term storage conditions.
doi_str_mv 10.1006/anbo.1999.0909
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source JSTOR Archive Collection A-Z Listing; Oxford University Press Journals All Titles (1996-Current); EZB-FREE-00999 freely available EZB journals; PubMed Central; Alma/SFX Local Collection
subjects assays
biochemical techniques
biomarkers
Brassicaceae
coincya rupestris
cold storage
Endemic species
Endemics
Endemics, genetic diversity, germination, isozymes, seed ageing, seed storage, vigour
enzyme activity
Genetic diversity
Genetic loci
genetic variation
Germination
Iberis
iberis pectinata
isozymes
loci
Moricandia
moricandia moricandioides
Plants
population
seed ageing
seed germination
seed longevity
seed moisture
Seed storage
Seeds
Species
storage
Storage conditions
temperature
vella pseudocytisus
Viability
vigor
vigour
title Evaluation of Seed Storage Conditions and Genetic Diversity of Four Crucifers Endemic to Spain
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