Platelet characteristics in patients with essential thrombocytosis
Background Essential thrombocytosis (ET) is a myeloproliferative disorder characterized by an increased platelet count. ET is associated with an increased risk of thrombosis, and procoagulant features of the disease may include an increased number of reactive reticulated platelets and an increased a...
Gespeichert in:
| Veröffentlicht in: | Cytometry. Part B, Clinical cytometry Clinical cytometry, 2018-11, Vol.94 (6), p.918-927 |
|---|---|
| Hauptverfasser: | , , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 927 |
|---|---|
| container_issue | 6 |
| container_start_page | 918 |
| container_title | Cytometry. Part B, Clinical cytometry |
| container_volume | 94 |
| creator | Pedersen, Oliver Heidmann Larsen, Mads Lamm Grove, Erik Lerkevang van Kooten Niekerk, Peter Buur Bønløkke, Søren Nissen, Peter H. Kristensen, Steen Dalby Hvas, Anne‐Mette |
| description | Background
Essential thrombocytosis (ET) is a myeloproliferative disorder characterized by an increased platelet count. ET is associated with an increased risk of thrombosis, and procoagulant features of the disease may include an increased number of reactive reticulated platelets and an increased aggregation potential. We aimed to explore the association between platelet count, platelet turnover, and platelet aggregation in patients with ET.
Methods
We included 24 ET patients who discontinued antiplatelet therapy prior to blood sampling. Reticulated platelets were assessed as immature platelet count (IPC) and immature platelet fraction by automated flow cytometry (Sysmex XE‐5000). Platelet aggregation was investigated by impedance aggregometry (Multiplate® Analyzer) and aggregation potential by flow cytometry (NAVIOS).
Results
Our results showed that ET patients had increased IPC compared to healthy individuals (median 12.3 vs. median 6.9, P |
| doi_str_mv | 10.1002/cyto.b.21642 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_2043172568</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2043172568</sourcerecordid><originalsourceid>FETCH-LOGICAL-c4022-c37a37dde002604e1e937e402c92692d3e38b3a0adbfefc6178a9fd59481f5543</originalsourceid><addsrcrecordid>eNp9kL1PwzAQxS0EoqWwMaNILAyk-DOJR1rxJVUqQxmYLMe5qK7SpNiuqv73uLR0YGC6O91P7949hK4JHhKM6YPZhm5YDinJOD1BfSIETbkU-emx57KHLrxfYMwEz_Jz1KMyl5iKrI9G740O0EBIzFw7bQI464M1PrFtstLBQht8srFhnoD3cbC6ScLcdcuy21321l-is1o3Hq4OdYA-np9m49d0Mn15Gz9OUsMxpalhuWZ5VUE0nWEOBCTLIa6MpJmkFQNWlExjXZU11CYjeaFlXQnJC1ILwdkA3e11V677WoMPamm9gabRLXRrryjmjOTxqyKit3_QRbd2bXSnKBGs4IJiFqn7PWVc572DWq2cXWq3VQSrXbZq96Eq1U-2Eb85iK7LJVRH-DfMCPA9sLENbP8VU-PP2XS01_0Gw_uFxg</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2153845203</pqid></control><display><type>article</type><title>Platelet characteristics in patients with essential thrombocytosis</title><source>Wiley Online Library Journals Frontfile Complete</source><source>Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals</source><source>Wiley Free Content</source><source>Alma/SFX Local Collection</source><creator>Pedersen, Oliver Heidmann ; Larsen, Mads Lamm ; Grove, Erik Lerkevang ; van Kooten Niekerk, Peter Buur ; Bønløkke, Søren ; Nissen, Peter H. ; Kristensen, Steen Dalby ; Hvas, Anne‐Mette</creator><creatorcontrib>Pedersen, Oliver Heidmann ; Larsen, Mads Lamm ; Grove, Erik Lerkevang ; van Kooten Niekerk, Peter Buur ; Bønløkke, Søren ; Nissen, Peter H. ; Kristensen, Steen Dalby ; Hvas, Anne‐Mette</creatorcontrib><description>Background
Essential thrombocytosis (ET) is a myeloproliferative disorder characterized by an increased platelet count. ET is associated with an increased risk of thrombosis, and procoagulant features of the disease may include an increased number of reactive reticulated platelets and an increased aggregation potential. We aimed to explore the association between platelet count, platelet turnover, and platelet aggregation in patients with ET.
Methods
We included 24 ET patients who discontinued antiplatelet therapy prior to blood sampling. Reticulated platelets were assessed as immature platelet count (IPC) and immature platelet fraction by automated flow cytometry (Sysmex XE‐5000). Platelet aggregation was investigated by impedance aggregometry (Multiplate® Analyzer) and aggregation potential by flow cytometry (NAVIOS).
Results
Our results showed that ET patients had increased IPC compared to healthy individuals (median 12.3 vs. median 6.9, P < 0.0001). Furthermore, a positive correlation between platelet count and impedance aggregation was demonstrated using arachidonic acid (r = 0.48, P = 0.02), thrombin‐receptor‐activating‐peptide (r = 0.46, P = 0.03) and adenosine diphosphate (r = 0.56, P = 0.007) as agonists. Finally, an increased aggregation potential was demonstrated in ET patients compared to healthy individuals.
Conclusions
The study showed that ET patients compared to healthy individuals have an increased amount of reticulated platelets and increased aggregation potential. These findings might in part explain the increased thromboembolic risk in patients with ET. © 2018 International Clinical Cytometry Society</description><identifier>ISSN: 1552-4949</identifier><identifier>EISSN: 1552-4957</identifier><identifier>DOI: 10.1002/cyto.b.21642</identifier><identifier>PMID: 29790256</identifier><language>eng</language><publisher>United States: Wiley Subscription Services, Inc</publisher><subject>Adenosine ; Adenosine diphosphate ; Agglomeration ; Antiplatelet therapy ; Arachidonic acid ; essential thrombocytosis ; Flow cytometry ; Impedance ; Myeloproliferative diseases ; Patients ; Platelet aggregation ; platelet function testing ; Platelets ; Thrombin ; Thrombocytosis ; Thromboembolism ; Thrombosis</subject><ispartof>Cytometry. Part B, Clinical cytometry, 2018-11, Vol.94 (6), p.918-927</ispartof><rights>2018 International Clinical Cytometry Society</rights><rights>2018 International Clinical Cytometry Society.</rights><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4022-c37a37dde002604e1e937e402c92692d3e38b3a0adbfefc6178a9fd59481f5543</citedby><cites>FETCH-LOGICAL-c4022-c37a37dde002604e1e937e402c92692d3e38b3a0adbfefc6178a9fd59481f5543</cites><orcidid>0000-0002-7437-270X</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Fcyto.b.21642$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Fcyto.b.21642$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1417,1433,27924,27925,45574,45575,46409,46833</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/29790256$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Pedersen, Oliver Heidmann</creatorcontrib><creatorcontrib>Larsen, Mads Lamm</creatorcontrib><creatorcontrib>Grove, Erik Lerkevang</creatorcontrib><creatorcontrib>van Kooten Niekerk, Peter Buur</creatorcontrib><creatorcontrib>Bønløkke, Søren</creatorcontrib><creatorcontrib>Nissen, Peter H.</creatorcontrib><creatorcontrib>Kristensen, Steen Dalby</creatorcontrib><creatorcontrib>Hvas, Anne‐Mette</creatorcontrib><title>Platelet characteristics in patients with essential thrombocytosis</title><title>Cytometry. Part B, Clinical cytometry</title><addtitle>Cytometry B Clin Cytom</addtitle><description>Background
Essential thrombocytosis (ET) is a myeloproliferative disorder characterized by an increased platelet count. ET is associated with an increased risk of thrombosis, and procoagulant features of the disease may include an increased number of reactive reticulated platelets and an increased aggregation potential. We aimed to explore the association between platelet count, platelet turnover, and platelet aggregation in patients with ET.
Methods
We included 24 ET patients who discontinued antiplatelet therapy prior to blood sampling. Reticulated platelets were assessed as immature platelet count (IPC) and immature platelet fraction by automated flow cytometry (Sysmex XE‐5000). Platelet aggregation was investigated by impedance aggregometry (Multiplate® Analyzer) and aggregation potential by flow cytometry (NAVIOS).
Results
Our results showed that ET patients had increased IPC compared to healthy individuals (median 12.3 vs. median 6.9, P < 0.0001). Furthermore, a positive correlation between platelet count and impedance aggregation was demonstrated using arachidonic acid (r = 0.48, P = 0.02), thrombin‐receptor‐activating‐peptide (r = 0.46, P = 0.03) and adenosine diphosphate (r = 0.56, P = 0.007) as agonists. Finally, an increased aggregation potential was demonstrated in ET patients compared to healthy individuals.
Conclusions
The study showed that ET patients compared to healthy individuals have an increased amount of reticulated platelets and increased aggregation potential. These findings might in part explain the increased thromboembolic risk in patients with ET. © 2018 International Clinical Cytometry Society</description><subject>Adenosine</subject><subject>Adenosine diphosphate</subject><subject>Agglomeration</subject><subject>Antiplatelet therapy</subject><subject>Arachidonic acid</subject><subject>essential thrombocytosis</subject><subject>Flow cytometry</subject><subject>Impedance</subject><subject>Myeloproliferative diseases</subject><subject>Patients</subject><subject>Platelet aggregation</subject><subject>platelet function testing</subject><subject>Platelets</subject><subject>Thrombin</subject><subject>Thrombocytosis</subject><subject>Thromboembolism</subject><subject>Thrombosis</subject><issn>1552-4949</issn><issn>1552-4957</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2018</creationdate><recordtype>article</recordtype><recordid>eNp9kL1PwzAQxS0EoqWwMaNILAyk-DOJR1rxJVUqQxmYLMe5qK7SpNiuqv73uLR0YGC6O91P7949hK4JHhKM6YPZhm5YDinJOD1BfSIETbkU-emx57KHLrxfYMwEz_Jz1KMyl5iKrI9G740O0EBIzFw7bQI464M1PrFtstLBQht8srFhnoD3cbC6ScLcdcuy21321l-is1o3Hq4OdYA-np9m49d0Mn15Gz9OUsMxpalhuWZ5VUE0nWEOBCTLIa6MpJmkFQNWlExjXZU11CYjeaFlXQnJC1ILwdkA3e11V677WoMPamm9gabRLXRrryjmjOTxqyKit3_QRbd2bXSnKBGs4IJiFqn7PWVc572DWq2cXWq3VQSrXbZq96Eq1U-2Eb85iK7LJVRH-DfMCPA9sLENbP8VU-PP2XS01_0Gw_uFxg</recordid><startdate>201811</startdate><enddate>201811</enddate><creator>Pedersen, Oliver Heidmann</creator><creator>Larsen, Mads Lamm</creator><creator>Grove, Erik Lerkevang</creator><creator>van Kooten Niekerk, Peter Buur</creator><creator>Bønløkke, Søren</creator><creator>Nissen, Peter H.</creator><creator>Kristensen, Steen Dalby</creator><creator>Hvas, Anne‐Mette</creator><general>Wiley Subscription Services, Inc</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>7T5</scope><scope>8FD</scope><scope>FR3</scope><scope>H94</scope><scope>P64</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0002-7437-270X</orcidid></search><sort><creationdate>201811</creationdate><title>Platelet characteristics in patients with essential thrombocytosis</title><author>Pedersen, Oliver Heidmann ; Larsen, Mads Lamm ; Grove, Erik Lerkevang ; van Kooten Niekerk, Peter Buur ; Bønløkke, Søren ; Nissen, Peter H. ; Kristensen, Steen Dalby ; Hvas, Anne‐Mette</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4022-c37a37dde002604e1e937e402c92692d3e38b3a0adbfefc6178a9fd59481f5543</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2018</creationdate><topic>Adenosine</topic><topic>Adenosine diphosphate</topic><topic>Agglomeration</topic><topic>Antiplatelet therapy</topic><topic>Arachidonic acid</topic><topic>essential thrombocytosis</topic><topic>Flow cytometry</topic><topic>Impedance</topic><topic>Myeloproliferative diseases</topic><topic>Patients</topic><topic>Platelet aggregation</topic><topic>platelet function testing</topic><topic>Platelets</topic><topic>Thrombin</topic><topic>Thrombocytosis</topic><topic>Thromboembolism</topic><topic>Thrombosis</topic><toplevel>online_resources</toplevel><creatorcontrib>Pedersen, Oliver Heidmann</creatorcontrib><creatorcontrib>Larsen, Mads Lamm</creatorcontrib><creatorcontrib>Grove, Erik Lerkevang</creatorcontrib><creatorcontrib>van Kooten Niekerk, Peter Buur</creatorcontrib><creatorcontrib>Bønløkke, Søren</creatorcontrib><creatorcontrib>Nissen, Peter H.</creatorcontrib><creatorcontrib>Kristensen, Steen Dalby</creatorcontrib><creatorcontrib>Hvas, Anne‐Mette</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>Biotechnology Research Abstracts</collection><collection>Immunology Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Cytometry. Part B, Clinical cytometry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Pedersen, Oliver Heidmann</au><au>Larsen, Mads Lamm</au><au>Grove, Erik Lerkevang</au><au>van Kooten Niekerk, Peter Buur</au><au>Bønløkke, Søren</au><au>Nissen, Peter H.</au><au>Kristensen, Steen Dalby</au><au>Hvas, Anne‐Mette</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Platelet characteristics in patients with essential thrombocytosis</atitle><jtitle>Cytometry. Part B, Clinical cytometry</jtitle><addtitle>Cytometry B Clin Cytom</addtitle><date>2018-11</date><risdate>2018</risdate><volume>94</volume><issue>6</issue><spage>918</spage><epage>927</epage><pages>918-927</pages><issn>1552-4949</issn><eissn>1552-4957</eissn><abstract>Background
Essential thrombocytosis (ET) is a myeloproliferative disorder characterized by an increased platelet count. ET is associated with an increased risk of thrombosis, and procoagulant features of the disease may include an increased number of reactive reticulated platelets and an increased aggregation potential. We aimed to explore the association between platelet count, platelet turnover, and platelet aggregation in patients with ET.
Methods
We included 24 ET patients who discontinued antiplatelet therapy prior to blood sampling. Reticulated platelets were assessed as immature platelet count (IPC) and immature platelet fraction by automated flow cytometry (Sysmex XE‐5000). Platelet aggregation was investigated by impedance aggregometry (Multiplate® Analyzer) and aggregation potential by flow cytometry (NAVIOS).
Results
Our results showed that ET patients had increased IPC compared to healthy individuals (median 12.3 vs. median 6.9, P < 0.0001). Furthermore, a positive correlation between platelet count and impedance aggregation was demonstrated using arachidonic acid (r = 0.48, P = 0.02), thrombin‐receptor‐activating‐peptide (r = 0.46, P = 0.03) and adenosine diphosphate (r = 0.56, P = 0.007) as agonists. Finally, an increased aggregation potential was demonstrated in ET patients compared to healthy individuals.
Conclusions
The study showed that ET patients compared to healthy individuals have an increased amount of reticulated platelets and increased aggregation potential. These findings might in part explain the increased thromboembolic risk in patients with ET. © 2018 International Clinical Cytometry Society</abstract><cop>United States</cop><pub>Wiley Subscription Services, Inc</pub><pmid>29790256</pmid><doi>10.1002/cyto.b.21642</doi><tpages>10</tpages><orcidid>https://orcid.org/0000-0002-7437-270X</orcidid><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1552-4949 |
| ispartof | Cytometry. Part B, Clinical cytometry, 2018-11, Vol.94 (6), p.918-927 |
| issn | 1552-4949 1552-4957 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_2043172568 |
| source | Wiley Online Library Journals Frontfile Complete; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Wiley Free Content; Alma/SFX Local Collection |
| subjects | Adenosine Adenosine diphosphate Agglomeration Antiplatelet therapy Arachidonic acid essential thrombocytosis Flow cytometry Impedance Myeloproliferative diseases Patients Platelet aggregation platelet function testing Platelets Thrombin Thrombocytosis Thromboembolism Thrombosis |
| title | Platelet characteristics in patients with essential thrombocytosis |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-06T19%3A06%3A57IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Platelet%20characteristics%20in%20patients%20with%20essential%20thrombocytosis&rft.jtitle=Cytometry.%20Part%20B,%20Clinical%20cytometry&rft.au=Pedersen,%20Oliver%20Heidmann&rft.date=2018-11&rft.volume=94&rft.issue=6&rft.spage=918&rft.epage=927&rft.pages=918-927&rft.issn=1552-4949&rft.eissn=1552-4957&rft_id=info:doi/10.1002/cyto.b.21642&rft_dat=%3Cproquest_cross%3E2043172568%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2153845203&rft_id=info:pmid/29790256&rfr_iscdi=true |