The addition of docosahexaenoic acid (DHA) and antioxidants (glutathione peroxidase and superoxide dismutase) in extenders to epididymal sperm cryopreservation in bulls
The cryopreservation of epididymal sperm is an important technique that allows genetic material to be preserved, even post mortem. However, cryopreservation leads to increased oxidative stress and impaired sperm viability. Polyunsaturated fatty acid (PUFA) supplementation may improve certain sperm c...
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| Veröffentlicht in: | Zygote (Cambridge) 2018-06, Vol.26 (3), p.199-206 |
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| creator | Losano, João D.A. Angrimani, Daniel S.R. Rui, Bruno R. Bicudo, Luana C. Dalmazzo, Andressa Silva, Bárbara C.S. Viana, Carlos H.C. Mendes, Camilla M. Assumpção, Mayra E.O.A. Barnabe, Valquiria H. Nichi, Marcilio |
| description | The cryopreservation of epididymal sperm is an important technique that allows genetic material to be preserved, even post mortem. However, cryopreservation leads to increased oxidative stress and impaired sperm viability. Polyunsaturated fatty acid (PUFA) supplementation may improve certain sperm characteristics, but it also makes sperm more susceptible to oxidative stress, therefore adding antioxidants that counteract oxidative stress has become an option. In this context, this study aimed to evaluate the effect of the interaction between docosahexaenoic acid (DHA) and antioxidants on the quality after the cryopreservation of epididymal bull sperm. Twenty epididymides were collected after slaughter, and epididymal sperm was cryopreserved with bovine extender supplemented with docosahexaenoic acid (DHA), glutathione peroxidase (GPx) and superoxide dismutase (SOD). We verified an improvement in motility in the group that was treated only with DHA 5 µM and a concentration-dependent effect on susceptibility to lipid peroxidation that was associated with DHA concentration (1 µM, 5 µM or 10 µM). Moreover, treatment with DHA (5 µM) and SOD (20 IU/ml) resulted in higher sperm motility. Thus, the association between DHA (5 µM) and SOD (20 IU/ml) appears to be an option for increased epididymal sperm features in bulls. |
| doi_str_mv | 10.1017/S0967199418000096 |
| format | Article |
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However, cryopreservation leads to increased oxidative stress and impaired sperm viability. Polyunsaturated fatty acid (PUFA) supplementation may improve certain sperm characteristics, but it also makes sperm more susceptible to oxidative stress, therefore adding antioxidants that counteract oxidative stress has become an option. In this context, this study aimed to evaluate the effect of the interaction between docosahexaenoic acid (DHA) and antioxidants on the quality after the cryopreservation of epididymal bull sperm. Twenty epididymides were collected after slaughter, and epididymal sperm was cryopreserved with bovine extender supplemented with docosahexaenoic acid (DHA), glutathione peroxidase (GPx) and superoxide dismutase (SOD). We verified an improvement in motility in the group that was treated only with DHA 5 µM and a concentration-dependent effect on susceptibility to lipid peroxidation that was associated with DHA concentration (1 µM, 5 µM or 10 µM). Moreover, treatment with DHA (5 µM) and SOD (20 IU/ml) resulted in higher sperm motility. 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However, cryopreservation leads to increased oxidative stress and impaired sperm viability. Polyunsaturated fatty acid (PUFA) supplementation may improve certain sperm characteristics, but it also makes sperm more susceptible to oxidative stress, therefore adding antioxidants that counteract oxidative stress has become an option. In this context, this study aimed to evaluate the effect of the interaction between docosahexaenoic acid (DHA) and antioxidants on the quality after the cryopreservation of epididymal bull sperm. Twenty epididymides were collected after slaughter, and epididymal sperm was cryopreserved with bovine extender supplemented with docosahexaenoic acid (DHA), glutathione peroxidase (GPx) and superoxide dismutase (SOD). We verified an improvement in motility in the group that was treated only with DHA 5 µM and a concentration-dependent effect on susceptibility to lipid peroxidation that was associated with DHA concentration (1 µM, 5 µM or 10 µM). 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Thus, the association between DHA (5 µM) and SOD (20 IU/ml) appears to be an option for increased epididymal sperm features in bulls.</description><subject>Animal sciences</subject><subject>Antioxidants</subject><subject>Conflicts of interest</subject><subject>Cryopreservation</subject><subject>Deoxyribonucleic acid</subject><subject>DNA</subject><subject>Docosahexaenoic acid</subject><subject>Experiments</subject><subject>Glutathione</subject><subject>Glutathione peroxidase</subject><subject>Lipid peroxidation</subject><subject>Lipids</subject><subject>Microscopy</subject><subject>Motility</subject><subject>Oxidative stress</subject><subject>Peroxidase</subject><subject>Peroxidation</subject><subject>Polyunsaturated fatty acids</subject><subject>Sperm</subject><subject>Superoxide dismutase</subject><subject>Supplements</subject><subject>Veterinary colleges</subject><subject>Veterinary 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(Cambridge)</jtitle><addtitle>Zygote</addtitle><date>2018-06</date><risdate>2018</risdate><volume>26</volume><issue>3</issue><spage>199</spage><epage>206</epage><pages>199-206</pages><issn>0967-1994</issn><eissn>1469-8730</eissn><abstract>The cryopreservation of epididymal sperm is an important technique that allows genetic material to be preserved, even post mortem. However, cryopreservation leads to increased oxidative stress and impaired sperm viability. Polyunsaturated fatty acid (PUFA) supplementation may improve certain sperm characteristics, but it also makes sperm more susceptible to oxidative stress, therefore adding antioxidants that counteract oxidative stress has become an option. In this context, this study aimed to evaluate the effect of the interaction between docosahexaenoic acid (DHA) and antioxidants on the quality after the cryopreservation of epididymal bull sperm. Twenty epididymides were collected after slaughter, and epididymal sperm was cryopreserved with bovine extender supplemented with docosahexaenoic acid (DHA), glutathione peroxidase (GPx) and superoxide dismutase (SOD). We verified an improvement in motility in the group that was treated only with DHA 5 µM and a concentration-dependent effect on susceptibility to lipid peroxidation that was associated with DHA concentration (1 µM, 5 µM or 10 µM). Moreover, treatment with DHA (5 µM) and SOD (20 IU/ml) resulted in higher sperm motility. Thus, the association between DHA (5 µM) and SOD (20 IU/ml) appears to be an option for increased epididymal sperm features in bulls.</abstract><cop>Cambridge, UK</cop><pub>Cambridge University Press</pub><pmid>29781410</pmid><doi>10.1017/S0967199418000096</doi><tpages>8</tpages></addata></record> |
| fulltext | fulltext |
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| ispartof | Zygote (Cambridge), 2018-06, Vol.26 (3), p.199-206 |
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| source | Cambridge Journals |
| subjects | Animal sciences Antioxidants Conflicts of interest Cryopreservation Deoxyribonucleic acid DNA Docosahexaenoic acid Experiments Glutathione Glutathione peroxidase Lipid peroxidation Lipids Microscopy Motility Oxidative stress Peroxidase Peroxidation Polyunsaturated fatty acids Sperm Superoxide dismutase Supplements Veterinary colleges Veterinary medicine Viability Zoology |
| title | The addition of docosahexaenoic acid (DHA) and antioxidants (glutathione peroxidase and superoxide dismutase) in extenders to epididymal sperm cryopreservation in bulls |
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