High-Throughput Screening Enhances Kidney Organoid Differentiation from Human Pluripotent Stem Cells and Enables Automated Multidimensional Phenotyping

Organoids derived from human pluripotent stem cells are a potentially powerful tool for high-throughput screening (HTS), but the complexity of organoid cultures poses a significant challenge for miniaturization and automation. Here, we present a fully automated, HTS-compatible platform for enhanced...

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Veröffentlicht in:	Cell stem cell 2018-06, Vol.22 (6), p.929-940.e4
Hauptverfasser:	Czerniecki, Stefan M., Cruz, Nelly M., Harder, Jennifer L., Menon, Rajasree, Annis, James, Otto, Edgar A., Gulieva, Ramila E., Islas, Laura V., Kim, Yong Kyun, Tran, Linh M., Martins, Timothy J., Pippin, Jeffrey W., Fu, Hongxia, Kretzler, Matthias, Shankland, Stuart J., Himmelfarb, Jonathan, Moon, Randall T., Paragas, Neal, Freedman, Benjamin S.
Format:	Artikel
Sprache:	eng
Schlagworte:	Automation Cell Culture Techniques Cell Differentiation High-Throughput Screening Assays Humans Kidney - cytology Organoids - cytology Phenotype Pluripotent Stem Cells - cytology Sequence Analysis, RNA
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