Preparation and Properties of Nanoparticles, tRNA–Bivalent Metal Cation (Me2+) Complexes, and Prospects of Their Practical Use
The patterns of formation of RNA nanoparticles (NPs) during thermal cycling of bacterial total tRNA in the presence of cations Ca 2+ , Mn 2+ , Ni 2+ , Zn 2+ , Co 2+ , and Cu 2+ were studied. The optimal conditions for the production of NPs were found, and it was revealed that their size depends on t...
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| Veröffentlicht in: | Doklady. Biochemistry and biophysics 2018-03, Vol.479 (1), p.118-122 |
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| container_title | Doklady. Biochemistry and biophysics |
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| creator | Danilevich, V. N. Sorokin, V. V. Moiseev, Ya. P. Sizova, S. V. |
| description | The patterns of formation of RNA nanoparticles (NPs) during thermal cycling of bacterial total tRNA in the presence of cations Ca
2+
, Mn
2+
, Ni
2+
, Zn
2+
, Co
2+
, and Cu
2+
were studied. The optimal conditions for the production of NPs were found, and it was revealed that their size depends on the ratio of the concentrations of Me
2+
and tRNA. The concentration of reagents for obtaining NPs of small size (from 5 to 100 nm) was selected. It was shown that tRNA-based nanoparticles can comprise short (20–50 nt) ribooligonucleotides, including aptamers and siRNAs. The stability of NPs during storage in buffer solutions of various composition was studied. It was found that the initial suspensions of NPs are quite stable, but they are rapidly destroyed in PBS buffer (pH 7.4). A simple and effective stabilizer (polyarginine) was found, the additives of which ensure the preservation of nanoparticles in PBS buffer for more than 5 h. Nanoparticles modified with the stabilizer are resistant to blood serum nucleases and can be used for transfection. |
| doi_str_mv | 10.1134/S1607672918020187 |
| format | Article |
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2+
, Mn
2+
, Ni
2+
, Zn
2+
, Co
2+
, and Cu
2+
were studied. The optimal conditions for the production of NPs were found, and it was revealed that their size depends on the ratio of the concentrations of Me
2+
and tRNA. The concentration of reagents for obtaining NPs of small size (from 5 to 100 nm) was selected. It was shown that tRNA-based nanoparticles can comprise short (20–50 nt) ribooligonucleotides, including aptamers and siRNAs. The stability of NPs during storage in buffer solutions of various composition was studied. It was found that the initial suspensions of NPs are quite stable, but they are rapidly destroyed in PBS buffer (pH 7.4). A simple and effective stabilizer (polyarginine) was found, the additives of which ensure the preservation of nanoparticles in PBS buffer for more than 5 h. Nanoparticles modified with the stabilizer are resistant to blood serum nucleases and can be used for transfection.</description><identifier>ISSN: 1607-6729</identifier><identifier>EISSN: 1608-3091</identifier><identifier>DOI: 10.1134/S1607672918020187</identifier><identifier>PMID: 29779113</identifier><language>eng</language><publisher>Moscow: Pleiades Publishing</publisher><subject>Additives ; Aptamers ; Biochemistry ; Biological and Medical Physics ; Biomedical and Life Sciences ; Biophysics ; Calcium ; Carbon dioxide ; Cations ; Cobalt ; Copper ; Life Sciences ; Metal ions ; Molecular Biology ; Nanoparticles ; Nuclease ; Preservation ; Reagents ; Ribonucleic acid ; RNA ; Shelf life ; siRNA ; Thermal cycling ; Transfection ; tRNA ; Zinc</subject><ispartof>Doklady. Biochemistry and biophysics, 2018-03, Vol.479 (1), p.118-122</ispartof><rights>Pleiades Publishing, Ltd. 2018</rights><rights>Doklady Biochemistry and Biophysics is a copyright of Springer, (2018). All Rights Reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c372t-9b1df3a60ec96f6746791b5a5ddbb1d1cbb466c0b15ec7798dc866da6d5eeab23</citedby><cites>FETCH-LOGICAL-c372t-9b1df3a60ec96f6746791b5a5ddbb1d1cbb466c0b15ec7798dc866da6d5eeab23</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1134/S1607672918020187$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1134/S1607672918020187$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,780,784,27924,27925,41488,42557,51319</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/29779113$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Danilevich, V. N.</creatorcontrib><creatorcontrib>Sorokin, V. V.</creatorcontrib><creatorcontrib>Moiseev, Ya. P.</creatorcontrib><creatorcontrib>Sizova, S. V.</creatorcontrib><title>Preparation and Properties of Nanoparticles, tRNA–Bivalent Metal Cation (Me2+) Complexes, and Prospects of Their Practical Use</title><title>Doklady. Biochemistry and biophysics</title><addtitle>Dokl Biochem Biophys</addtitle><addtitle>Dokl Biochem Biophys</addtitle><description>The patterns of formation of RNA nanoparticles (NPs) during thermal cycling of bacterial total tRNA in the presence of cations Ca
2+
, Mn
2+
, Ni
2+
, Zn
2+
, Co
2+
, and Cu
2+
were studied. The optimal conditions for the production of NPs were found, and it was revealed that their size depends on the ratio of the concentrations of Me
2+
and tRNA. The concentration of reagents for obtaining NPs of small size (from 5 to 100 nm) was selected. It was shown that tRNA-based nanoparticles can comprise short (20–50 nt) ribooligonucleotides, including aptamers and siRNAs. The stability of NPs during storage in buffer solutions of various composition was studied. It was found that the initial suspensions of NPs are quite stable, but they are rapidly destroyed in PBS buffer (pH 7.4). A simple and effective stabilizer (polyarginine) was found, the additives of which ensure the preservation of nanoparticles in PBS buffer for more than 5 h. Nanoparticles modified with the stabilizer are resistant to blood serum nucleases and can be used for transfection.</description><subject>Additives</subject><subject>Aptamers</subject><subject>Biochemistry</subject><subject>Biological and Medical Physics</subject><subject>Biomedical and Life Sciences</subject><subject>Biophysics</subject><subject>Calcium</subject><subject>Carbon dioxide</subject><subject>Cations</subject><subject>Cobalt</subject><subject>Copper</subject><subject>Life Sciences</subject><subject>Metal ions</subject><subject>Molecular Biology</subject><subject>Nanoparticles</subject><subject>Nuclease</subject><subject>Preservation</subject><subject>Reagents</subject><subject>Ribonucleic acid</subject><subject>RNA</subject><subject>Shelf life</subject><subject>siRNA</subject><subject>Thermal cycling</subject><subject>Transfection</subject><subject>tRNA</subject><subject>Zinc</subject><issn>1607-6729</issn><issn>1608-3091</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2018</creationdate><recordtype>article</recordtype><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNp1kU1OwzAQhS0EglI4ABsUiU0RBGwnsZNlqfiTSqn4WUeOM4GgNAm2i2DHHbghJ2FKC0ggVrbmfe_ZT0PIFqMHjAXh4TUTVArJExZTTlksl0gHR7Ef0IQtf96lP9PXyLq1DxQhHkSrZI0nUiYY0SGvYwOtMsqVTe2pOvfGpmnBuBKs1xTeSNUNyq7UFdh9z12N-u-vb0flk6qgdt4FOFV5g7m7dwF8b9cbNJO2gucZvsizLWj3GXdzD6XBkdKYiM5bCxtkpVCVhc3F2SW3J8c3gzN_eHl6PugPfR1I7vwkY3kRKEFBJ6IQMhRYIItUlOcZSkxnWSiEphmLQGO5ONexELkSeQSgMh50SW-e25rmcQrWpZPSaqgqVUMztSmnIedBEDKK6M4v9KGZmhp_N6MYj6RAskvYnNLY0Boo0taUE2VeUkbT2XrSP-tBz_YieZpNIP92fO0DAT4HLEr1HZifp_9P_QCRu5pT</recordid><startdate>20180301</startdate><enddate>20180301</enddate><creator>Danilevich, V. N.</creator><creator>Sorokin, V. V.</creator><creator>Moiseev, Ya. P.</creator><creator>Sizova, S. V.</creator><general>Pleiades Publishing</general><general>Springer Nature B.V</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QP</scope><scope>7QR</scope><scope>7T5</scope><scope>7TK</scope><scope>7TM</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>88I</scope><scope>8AO</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M2P</scope><scope>M7P</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>Q9U</scope><scope>7X8</scope></search><sort><creationdate>20180301</creationdate><title>Preparation and Properties of Nanoparticles, tRNA–Bivalent Metal Cation (Me2+) Complexes, and Prospects of Their Practical Use</title><author>Danilevich, V. N. ; Sorokin, V. V. ; Moiseev, Ya. P. ; Sizova, S. V.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c372t-9b1df3a60ec96f6746791b5a5ddbb1d1cbb466c0b15ec7798dc866da6d5eeab23</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2018</creationdate><topic>Additives</topic><topic>Aptamers</topic><topic>Biochemistry</topic><topic>Biological and Medical Physics</topic><topic>Biomedical and Life Sciences</topic><topic>Biophysics</topic><topic>Calcium</topic><topic>Carbon dioxide</topic><topic>Cations</topic><topic>Cobalt</topic><topic>Copper</topic><topic>Life Sciences</topic><topic>Metal ions</topic><topic>Molecular Biology</topic><topic>Nanoparticles</topic><topic>Nuclease</topic><topic>Preservation</topic><topic>Reagents</topic><topic>Ribonucleic acid</topic><topic>RNA</topic><topic>Shelf life</topic><topic>siRNA</topic><topic>Thermal cycling</topic><topic>Transfection</topic><topic>tRNA</topic><topic>Zinc</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Danilevich, V. N.</creatorcontrib><creatorcontrib>Sorokin, V. V.</creatorcontrib><creatorcontrib>Moiseev, Ya. P.</creatorcontrib><creatorcontrib>Sizova, S. 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Biochemistry and biophysics</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Danilevich, V. N.</au><au>Sorokin, V. V.</au><au>Moiseev, Ya. P.</au><au>Sizova, S. V.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Preparation and Properties of Nanoparticles, tRNA–Bivalent Metal Cation (Me2+) Complexes, and Prospects of Their Practical Use</atitle><jtitle>Doklady. Biochemistry and biophysics</jtitle><stitle>Dokl Biochem Biophys</stitle><addtitle>Dokl Biochem Biophys</addtitle><date>2018-03-01</date><risdate>2018</risdate><volume>479</volume><issue>1</issue><spage>118</spage><epage>122</epage><pages>118-122</pages><issn>1607-6729</issn><eissn>1608-3091</eissn><abstract>The patterns of formation of RNA nanoparticles (NPs) during thermal cycling of bacterial total tRNA in the presence of cations Ca
2+
, Mn
2+
, Ni
2+
, Zn
2+
, Co
2+
, and Cu
2+
were studied. The optimal conditions for the production of NPs were found, and it was revealed that their size depends on the ratio of the concentrations of Me
2+
and tRNA. The concentration of reagents for obtaining NPs of small size (from 5 to 100 nm) was selected. It was shown that tRNA-based nanoparticles can comprise short (20–50 nt) ribooligonucleotides, including aptamers and siRNAs. The stability of NPs during storage in buffer solutions of various composition was studied. It was found that the initial suspensions of NPs are quite stable, but they are rapidly destroyed in PBS buffer (pH 7.4). A simple and effective stabilizer (polyarginine) was found, the additives of which ensure the preservation of nanoparticles in PBS buffer for more than 5 h. Nanoparticles modified with the stabilizer are resistant to blood serum nucleases and can be used for transfection.</abstract><cop>Moscow</cop><pub>Pleiades Publishing</pub><pmid>29779113</pmid><doi>10.1134/S1607672918020187</doi><tpages>5</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1607-6729 |
| ispartof | Doklady. Biochemistry and biophysics, 2018-03, Vol.479 (1), p.118-122 |
| issn | 1607-6729 1608-3091 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_2042233410 |
| source | SpringerLink Journals - AutoHoldings |
| subjects | Additives Aptamers Biochemistry Biological and Medical Physics Biomedical and Life Sciences Biophysics Calcium Carbon dioxide Cations Cobalt Copper Life Sciences Metal ions Molecular Biology Nanoparticles Nuclease Preservation Reagents Ribonucleic acid RNA Shelf life siRNA Thermal cycling Transfection tRNA Zinc |
| title | Preparation and Properties of Nanoparticles, tRNA–Bivalent Metal Cation (Me2+) Complexes, and Prospects of Their Practical Use |
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