Plant regeneration of common ash (Fraxinus excelsior L.) by somatic embryogenesis
This is the first report on somatic embryogenesis in common ash (Fraxinus excelsior L.). Experiments on somatic embryogenesis induction were carried out on zygotic embryos at different phases of development and maturation. The embryo axes were isolated and cultured on media containing different plan...
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| Veröffentlicht in: | In vitro cellular & developmental biology. Plant 2007-03, Vol.43 (2), p.101-110 |
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| creator | Capuana, M. Petrini, G. Di Marco, A. Giannini, R. |
| description | This is the first report on somatic embryogenesis in common ash (Fraxinus excelsior L.). Experiments on somatic embryogenesis induction were carried out on zygotic embryos at different phases of development and maturation. The embryo axes were isolated and cultured on media containing different plant growth regulators (PGRs). Embryogenic tissues were obtained from embryos collected at an incomplete maturation phase and cultured on a modified Murashige and Skoog medium containing 8.8 μM 2, 4-dichlorophenoxyacetic acid and 4.4 μM benzyl-adenine (BA). Embryos isolated from seeds at an advanced stage of maturation showed only organogenetic phenomena. Embryogenic tissues were successfully subcultured and multiplied on medium containing a reduced concentration of PGRs. After their isolation, somatic embryos were induced to develop and mature by transfer to PGR-free medium and subsequent culture on medium containing 0.1 μM BA. Somatic embryos developed completely and also germinated spontaneously. Embryo germination and conversion were significantly improved when subjected to a period of storage at 4°C and transplant onto woody plant medium. Plantlets were successfully transferred to soil and acclimatized in a “misted” greenhouse. |
| doi_str_mv | 10.1007/s11627-007-9030-0 |
| format | Article |
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Experiments on somatic embryogenesis induction were carried out on zygotic embryos at different phases of development and maturation. The embryo axes were isolated and cultured on media containing different plant growth regulators (PGRs). Embryogenic tissues were obtained from embryos collected at an incomplete maturation phase and cultured on a modified Murashige and Skoog medium containing 8.8 μM 2, 4-dichlorophenoxyacetic acid and 4.4 μM benzyl-adenine (BA). Embryos isolated from seeds at an advanced stage of maturation showed only organogenetic phenomena. Embryogenic tissues were successfully subcultured and multiplied on medium containing a reduced concentration of PGRs. After their isolation, somatic embryos were induced to develop and mature by transfer to PGR-free medium and subsequent culture on medium containing 0.1 μM BA. Somatic embryos developed completely and also germinated spontaneously. Embryo germination and conversion were significantly improved when subjected to a period of storage at 4°C and transplant onto woody plant medium. Plantlets were successfully transferred to soil and acclimatized in a “misted” greenhouse.</description><identifier>ISSN: 1054-5476</identifier><identifier>EISSN: 1475-2689</identifier><identifier>DOI: 10.1007/s11627-007-9030-0</identifier><language>eng</language><publisher>Heidelberg: The Society for In Vitro Biology</publisher><subject>Acclimatization ; Cold storage ; Cotyledons ; culture media ; Embryogenesis ; Embryological stage ; Embryonic growth stage ; Embryos ; Fraxinus excelsior ; Genetic engineering ; Germination ; greenhouses ; Growth regulators ; Immature zygotic embryo ; Plant cells ; Plant growth ; Plant growth regulators ; plant growth substances ; Plant reproduction ; Plantlets ; RESEARCH REPORTS ; Seeds ; Somatic embryogenesis ; Somatic embryos ; storage time ; tissues ; Trees ; Woody plants</subject><ispartof>In vitro cellular & developmental biology. Plant, 2007-03, Vol.43 (2), p.101-110</ispartof><rights>The Society for In Vitro Biology 2007</rights><rights>Copyright 2007 Springer Science+Business Media, LLC</rights><rights>Copyright Society for In Vitro Biology Mar/Apr 2007</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-b389t-d6bc3b64ca5d1229e27dbac2ee8be09259d442d3f7c8c65c630379ec951df4e13</citedby><cites>FETCH-LOGICAL-b389t-d6bc3b64ca5d1229e27dbac2ee8be09259d442d3f7c8c65c630379ec951df4e13</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://bioone.org/doi/pdf/10.1007/s11627-007-9030-0$$EPDF$$P50$$Gbioone$$H</linktopdf><linktohtml>$$Uhttps://www.jstor.org/stable/20461656$$EHTML$$P50$$Gjstor$$H</linktohtml><link.rule.ids>314,780,784,803,26978,27924,27925,52363,58017,58250</link.rule.ids></links><search><contributor>Yeung, Ed</contributor><creatorcontrib>Capuana, M.</creatorcontrib><creatorcontrib>Petrini, G.</creatorcontrib><creatorcontrib>Di Marco, A.</creatorcontrib><creatorcontrib>Giannini, R.</creatorcontrib><title>Plant regeneration of common ash (Fraxinus excelsior L.) by somatic embryogenesis</title><title>In vitro cellular & developmental biology. Plant</title><description>This is the first report on somatic embryogenesis in common ash (Fraxinus excelsior L.). Experiments on somatic embryogenesis induction were carried out on zygotic embryos at different phases of development and maturation. The embryo axes were isolated and cultured on media containing different plant growth regulators (PGRs). Embryogenic tissues were obtained from embryos collected at an incomplete maturation phase and cultured on a modified Murashige and Skoog medium containing 8.8 μM 2, 4-dichlorophenoxyacetic acid and 4.4 μM benzyl-adenine (BA). Embryos isolated from seeds at an advanced stage of maturation showed only organogenetic phenomena. Embryogenic tissues were successfully subcultured and multiplied on medium containing a reduced concentration of PGRs. After their isolation, somatic embryos were induced to develop and mature by transfer to PGR-free medium and subsequent culture on medium containing 0.1 μM BA. Somatic embryos developed completely and also germinated spontaneously. Embryo germination and conversion were significantly improved when subjected to a period of storage at 4°C and transplant onto woody plant medium. Plantlets were successfully transferred to soil and acclimatized in a “misted” greenhouse.</description><subject>Acclimatization</subject><subject>Cold storage</subject><subject>Cotyledons</subject><subject>culture media</subject><subject>Embryogenesis</subject><subject>Embryological stage</subject><subject>Embryonic growth stage</subject><subject>Embryos</subject><subject>Fraxinus excelsior</subject><subject>Genetic engineering</subject><subject>Germination</subject><subject>greenhouses</subject><subject>Growth regulators</subject><subject>Immature zygotic embryo</subject><subject>Plant cells</subject><subject>Plant growth</subject><subject>Plant growth regulators</subject><subject>plant growth substances</subject><subject>Plant reproduction</subject><subject>Plantlets</subject><subject>RESEARCH REPORTS</subject><subject>Seeds</subject><subject>Somatic embryogenesis</subject><subject>Somatic embryos</subject><subject>storage time</subject><subject>tissues</subject><subject>Trees</subject><subject>Woody plants</subject><issn>1054-5476</issn><issn>1475-2689</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2007</creationdate><recordtype>article</recordtype><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNqNUE1L5UAQDLKCrvoDPMgOexD3EO2ZzMebo4juCg9U1PMwmXQ0j5eMTueB7987IYuHPe2pC7qqurqK4pjDOQcwF8S5FqbMsLRQQQk7xT6XRpVCL-y3jEHJUkmj94rvRCsA4MDNfvFwv_bDyBK-4IDJj10cWGxZiH2fkadXdnaT_Ec3bIjhR8A1dTGx5fkvVm8ZxT4rAsO-Tts4OVBHh8Vu69eER3_nQfF8c_109adc3v2-vbpclnW1sGPZ6DpUtZbBq4YLYVGYpvZBIC5qBCuUbaQUTdWasAhaBV1BZSwGq3jTSuTVQXE6-76l-L5BGl3fUQ6Y_8G4ISdAciNAZeLPf4iruElDzua4NRKUUJMbn0khRaKErXtLXe_T1nFwU8NubthNcGrYQdaczJoVjTF9CfJhzbXSef9j3rc-Ov-SOnLPjwK4AhD5GZgcLmZG3cU44H_c_ATMq48Z</recordid><startdate>20070301</startdate><enddate>20070301</enddate><creator>Capuana, M.</creator><creator>Petrini, G.</creator><creator>Di Marco, A.</creator><creator>Giannini, R.</creator><general>The Society for In Vitro Biology</general><general>Springer</general><general>Springer Nature B.V</general><scope>FBQ</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>4T-</scope><scope>4U-</scope><scope>7X2</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88I</scope><scope>8AF</scope><scope>8AO</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0K</scope><scope>M0S</scope><scope>M2P</scope><scope>M7P</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>Q9U</scope><scope>S0X</scope><scope>7QO</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope></search><sort><creationdate>20070301</creationdate><title>Plant regeneration of common ash (Fraxinus excelsior L.) by somatic embryogenesis</title><author>Capuana, M. ; Petrini, G. ; Di Marco, A. ; Giannini, R.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-b389t-d6bc3b64ca5d1229e27dbac2ee8be09259d442d3f7c8c65c630379ec951df4e13</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2007</creationdate><topic>Acclimatization</topic><topic>Cold storage</topic><topic>Cotyledons</topic><topic>culture media</topic><topic>Embryogenesis</topic><topic>Embryological stage</topic><topic>Embryonic growth stage</topic><topic>Embryos</topic><topic>Fraxinus excelsior</topic><topic>Genetic engineering</topic><topic>Germination</topic><topic>greenhouses</topic><topic>Growth regulators</topic><topic>Immature zygotic embryo</topic><topic>Plant cells</topic><topic>Plant growth</topic><topic>Plant growth regulators</topic><topic>plant growth substances</topic><topic>Plant reproduction</topic><topic>Plantlets</topic><topic>RESEARCH REPORTS</topic><topic>Seeds</topic><topic>Somatic embryogenesis</topic><topic>Somatic embryos</topic><topic>storage time</topic><topic>tissues</topic><topic>Trees</topic><topic>Woody plants</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Capuana, M.</creatorcontrib><creatorcontrib>Petrini, G.</creatorcontrib><creatorcontrib>Di Marco, A.</creatorcontrib><creatorcontrib>Giannini, R.</creatorcontrib><collection>AGRIS</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Docstoc</collection><collection>University Readers</collection><collection>Agricultural Science Collection</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Science Database (Alumni Edition)</collection><collection>STEM Database</collection><collection>ProQuest Pharma Collection</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>Agricultural & Environmental Science Collection</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Agricultural Science Database</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Science Database</collection><collection>Biological Science Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central Basic</collection><collection>SIRS Editorial</collection><collection>Biotechnology Research Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><jtitle>In vitro cellular & developmental biology. Plant</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Capuana, M.</au><au>Petrini, G.</au><au>Di Marco, A.</au><au>Giannini, R.</au><au>Yeung, Ed</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Plant regeneration of common ash (Fraxinus excelsior L.) by somatic embryogenesis</atitle><jtitle>In vitro cellular & developmental biology. Plant</jtitle><date>2007-03-01</date><risdate>2007</risdate><volume>43</volume><issue>2</issue><spage>101</spage><epage>110</epage><pages>101-110</pages><issn>1054-5476</issn><eissn>1475-2689</eissn><abstract>This is the first report on somatic embryogenesis in common ash (Fraxinus excelsior L.). Experiments on somatic embryogenesis induction were carried out on zygotic embryos at different phases of development and maturation. The embryo axes were isolated and cultured on media containing different plant growth regulators (PGRs). Embryogenic tissues were obtained from embryos collected at an incomplete maturation phase and cultured on a modified Murashige and Skoog medium containing 8.8 μM 2, 4-dichlorophenoxyacetic acid and 4.4 μM benzyl-adenine (BA). Embryos isolated from seeds at an advanced stage of maturation showed only organogenetic phenomena. Embryogenic tissues were successfully subcultured and multiplied on medium containing a reduced concentration of PGRs. After their isolation, somatic embryos were induced to develop and mature by transfer to PGR-free medium and subsequent culture on medium containing 0.1 μM BA. Somatic embryos developed completely and also germinated spontaneously. Embryo germination and conversion were significantly improved when subjected to a period of storage at 4°C and transplant onto woody plant medium. Plantlets were successfully transferred to soil and acclimatized in a “misted” greenhouse.</abstract><cop>Heidelberg</cop><pub>The Society for In Vitro Biology</pub><doi>10.1007/s11627-007-9030-0</doi><tpages>10</tpages></addata></record> |
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| ispartof | In vitro cellular & developmental biology. Plant, 2007-03, Vol.43 (2), p.101-110 |
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| subjects | Acclimatization Cold storage Cotyledons culture media Embryogenesis Embryological stage Embryonic growth stage Embryos Fraxinus excelsior Genetic engineering Germination greenhouses Growth regulators Immature zygotic embryo Plant cells Plant growth Plant growth regulators plant growth substances Plant reproduction Plantlets RESEARCH REPORTS Seeds Somatic embryogenesis Somatic embryos storage time tissues Trees Woody plants |
| title | Plant regeneration of common ash (Fraxinus excelsior L.) by somatic embryogenesis |
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