Assessment of cytotoxicity and DNA damage exhibited by siloranes and oxiranes in cultured mammalian cells
The potential reactivity and structural properties of oxiranes (epoxides) are advantageous when considering polymers for medical devices. However, epoxy compounds are widely known to have genotoxic properties. The objective of the study was to evaluate the cytotoxicity and primary DNA damage effects...
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| Veröffentlicht in: | Mutation research 2007-12, Vol.634 (1), p.156-162 |
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| creator | Kostoryz, E.L. Zhu, Q. Zhao, Hong Glaros, A.G. Eick, J.D. |
| description | The potential reactivity and structural properties of oxiranes (epoxides) are advantageous when considering polymers for medical devices. However, epoxy compounds are widely known to have genotoxic properties. The objective of the study was to evaluate the cytotoxicity and primary DNA damage effects induced by oxiranes and siloranes, silicon containing oxiranes. The siloranes, Ph-Sil, Tet-Sil, and Sil-Mix and the oxiranes Cyracure™ UVR-6105 and 1,3-bis[2-(2-oxiranylmethyl) phenoxy]pentane (OMP-5) were dissolved in organic solvents and dilutions containing less than 0.5% solvent were used in biological assays. The concentration that reduced the viability of 50% (TC
50) of L929 cells was measured using the MTT assay and guided the selection of subtoxic doses for evaluation of DNA damage. Ph-Sil was more cytotoxic than OMP-5, Cyracure™ UVR-6105 and Sil-Mix. However, the TC
50 value of Tet-Sil could not be determined due to its poor solubility. DNA damage was evaluated in the sister chromatid exchange (SCE) assay with CHO cells, and the alkaline comet assay with L929 cells. In contrast to the siloranes, the oxiranes exhibited significant increases (
p
>
0.05) in SCE frequencies and DNA migration relative to their solvent controls. Our findings support previous reports that siloranes have low genotoxic potential and can be suitable components for development of biomaterials. |
| doi_str_mv | 10.1016/j.mrgentox.2007.07.003 |
| format | Article |
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50) of L929 cells was measured using the MTT assay and guided the selection of subtoxic doses for evaluation of DNA damage. Ph-Sil was more cytotoxic than OMP-5, Cyracure™ UVR-6105 and Sil-Mix. However, the TC
50 value of Tet-Sil could not be determined due to its poor solubility. DNA damage was evaluated in the sister chromatid exchange (SCE) assay with CHO cells, and the alkaline comet assay with L929 cells. In contrast to the siloranes, the oxiranes exhibited significant increases (
p
>
0.05) in SCE frequencies and DNA migration relative to their solvent controls. Our findings support previous reports that siloranes have low genotoxic potential and can be suitable components for development of biomaterials.</description><identifier>ISSN: 1383-5718</identifier><identifier>ISSN: 0027-5107</identifier><identifier>EISSN: 1879-3592</identifier><identifier>DOI: 10.1016/j.mrgentox.2007.07.003</identifier><identifier>PMID: 17719836</identifier><language>eng</language><publisher>Amsterdam: Elsevier B.V</publisher><subject>Animals ; Biological and medical sciences ; Cell culture ; Cell Survival - drug effects ; CHO Cells ; Comet Assay ; Cricetinae ; Cricetulus ; DNA damage ; DNA Damage - drug effects ; Epoxy Resins - toxicity ; Ethylene Oxide - analogs & derivatives ; Ethylene Oxide - chemistry ; Ethylene Oxide - toxicity ; Fundamental and applied biological sciences. Psychology ; Genetics of eukaryotes. Biological and molecular evolution ; Medical sciences ; Mice ; Oxiranes ; Silicon ; Silicon Compounds - toxicity ; Siloranes ; Sister Chromatid Exchange ; Sister chromatid exchanges ; Toxicology</subject><ispartof>Mutation research, 2007-12, Vol.634 (1), p.156-162</ispartof><rights>2007</rights><rights>2008 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c427t-66f126807e7a586c6fb41b99f1a59c15d925be6e46ec0a17bf4e791702f9d2013</citedby><cites>FETCH-LOGICAL-c427t-66f126807e7a586c6fb41b99f1a59c15d925be6e46ec0a17bf4e791702f9d2013</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.mrgentox.2007.07.003$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=19371748$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/17719836$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Kostoryz, E.L.</creatorcontrib><creatorcontrib>Zhu, Q.</creatorcontrib><creatorcontrib>Zhao, Hong</creatorcontrib><creatorcontrib>Glaros, A.G.</creatorcontrib><creatorcontrib>Eick, J.D.</creatorcontrib><title>Assessment of cytotoxicity and DNA damage exhibited by siloranes and oxiranes in cultured mammalian cells</title><title>Mutation research</title><addtitle>Mutat Res</addtitle><description>The potential reactivity and structural properties of oxiranes (epoxides) are advantageous when considering polymers for medical devices. However, epoxy compounds are widely known to have genotoxic properties. The objective of the study was to evaluate the cytotoxicity and primary DNA damage effects induced by oxiranes and siloranes, silicon containing oxiranes. The siloranes, Ph-Sil, Tet-Sil, and Sil-Mix and the oxiranes Cyracure™ UVR-6105 and 1,3-bis[2-(2-oxiranylmethyl) phenoxy]pentane (OMP-5) were dissolved in organic solvents and dilutions containing less than 0.5% solvent were used in biological assays. The concentration that reduced the viability of 50% (TC
50) of L929 cells was measured using the MTT assay and guided the selection of subtoxic doses for evaluation of DNA damage. Ph-Sil was more cytotoxic than OMP-5, Cyracure™ UVR-6105 and Sil-Mix. However, the TC
50 value of Tet-Sil could not be determined due to its poor solubility. DNA damage was evaluated in the sister chromatid exchange (SCE) assay with CHO cells, and the alkaline comet assay with L929 cells. In contrast to the siloranes, the oxiranes exhibited significant increases (
p
>
0.05) in SCE frequencies and DNA migration relative to their solvent controls. Our findings support previous reports that siloranes have low genotoxic potential and can be suitable components for development of biomaterials.</description><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Cell culture</subject><subject>Cell Survival - drug effects</subject><subject>CHO Cells</subject><subject>Comet Assay</subject><subject>Cricetinae</subject><subject>Cricetulus</subject><subject>DNA damage</subject><subject>DNA Damage - drug effects</subject><subject>Epoxy Resins - toxicity</subject><subject>Ethylene Oxide - analogs & derivatives</subject><subject>Ethylene Oxide - chemistry</subject><subject>Ethylene Oxide - toxicity</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Genetics of eukaryotes. Biological and molecular evolution</subject><subject>Medical sciences</subject><subject>Mice</subject><subject>Oxiranes</subject><subject>Silicon</subject><subject>Silicon Compounds - toxicity</subject><subject>Siloranes</subject><subject>Sister Chromatid Exchange</subject><subject>Sister chromatid exchanges</subject><subject>Toxicology</subject><issn>1383-5718</issn><issn>0027-5107</issn><issn>1879-3592</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2007</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkEtv1DAUhS1URB_wFypvYJfBj8SOdx0VWpAq2MDacpzr4lGcFN8Edf49TmeqLitdyQ9959yjQ8glZxvOuPq826R8D-M8PW4EY3qzDpNvyBlvtalkY8RJuctWVo3m7Sk5R9wxJphk7TtyyrXmppXqjMQtIiCmYkWnQP1-nopn9HHeUzf29MuPLe1dcvdA4fFP7OIMPe32FOMwZTcCPlFFcXjEkfplmJdcqORSckN05QuGAd-Tt8ENCB-O5wX5ffP11_W36u7n7ffr7V3la6HnSqnAhWqZBu2aVnkVupp3xgTuGuN50xvRdKCgVuCZ47oLNWjDNRPB9IJxeUE-HXwf8vR3AZxtirgmKAGnBa1gNVdFUUB1AH2eEDME-5BjcnlvObNryXZnn0u2a8l2HSaL8PK4YekS9C-yY6sF-HgEHHo3hNKNj_jCGam5rtvCXR04KH38i5At-gijhz5m8LPtp_halv84aZ-p</recordid><startdate>20071201</startdate><enddate>20071201</enddate><creator>Kostoryz, E.L.</creator><creator>Zhu, Q.</creator><creator>Zhao, Hong</creator><creator>Glaros, A.G.</creator><creator>Eick, J.D.</creator><general>Elsevier B.V</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TM</scope><scope>7U7</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope></search><sort><creationdate>20071201</creationdate><title>Assessment of cytotoxicity and DNA damage exhibited by siloranes and oxiranes in cultured mammalian cells</title><author>Kostoryz, E.L. ; Zhu, Q. ; Zhao, Hong ; Glaros, A.G. ; Eick, J.D.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c427t-66f126807e7a586c6fb41b99f1a59c15d925be6e46ec0a17bf4e791702f9d2013</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2007</creationdate><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Cell culture</topic><topic>Cell Survival - drug effects</topic><topic>CHO Cells</topic><topic>Comet Assay</topic><topic>Cricetinae</topic><topic>Cricetulus</topic><topic>DNA damage</topic><topic>DNA Damage - drug effects</topic><topic>Epoxy Resins - toxicity</topic><topic>Ethylene Oxide - analogs & derivatives</topic><topic>Ethylene Oxide - chemistry</topic><topic>Ethylene Oxide - toxicity</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Genetics of eukaryotes. Biological and molecular evolution</topic><topic>Medical sciences</topic><topic>Mice</topic><topic>Oxiranes</topic><topic>Silicon</topic><topic>Silicon Compounds - toxicity</topic><topic>Siloranes</topic><topic>Sister Chromatid Exchange</topic><topic>Sister chromatid exchanges</topic><topic>Toxicology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Kostoryz, E.L.</creatorcontrib><creatorcontrib>Zhu, Q.</creatorcontrib><creatorcontrib>Zhao, Hong</creatorcontrib><creatorcontrib>Glaros, A.G.</creatorcontrib><creatorcontrib>Eick, J.D.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Nucleic Acids Abstracts</collection><collection>Toxicology Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><jtitle>Mutation research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kostoryz, E.L.</au><au>Zhu, Q.</au><au>Zhao, Hong</au><au>Glaros, A.G.</au><au>Eick, J.D.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Assessment of cytotoxicity and DNA damage exhibited by siloranes and oxiranes in cultured mammalian cells</atitle><jtitle>Mutation research</jtitle><addtitle>Mutat Res</addtitle><date>2007-12-01</date><risdate>2007</risdate><volume>634</volume><issue>1</issue><spage>156</spage><epage>162</epage><pages>156-162</pages><issn>1383-5718</issn><issn>0027-5107</issn><eissn>1879-3592</eissn><abstract>The potential reactivity and structural properties of oxiranes (epoxides) are advantageous when considering polymers for medical devices. However, epoxy compounds are widely known to have genotoxic properties. The objective of the study was to evaluate the cytotoxicity and primary DNA damage effects induced by oxiranes and siloranes, silicon containing oxiranes. The siloranes, Ph-Sil, Tet-Sil, and Sil-Mix and the oxiranes Cyracure™ UVR-6105 and 1,3-bis[2-(2-oxiranylmethyl) phenoxy]pentane (OMP-5) were dissolved in organic solvents and dilutions containing less than 0.5% solvent were used in biological assays. The concentration that reduced the viability of 50% (TC
50) of L929 cells was measured using the MTT assay and guided the selection of subtoxic doses for evaluation of DNA damage. Ph-Sil was more cytotoxic than OMP-5, Cyracure™ UVR-6105 and Sil-Mix. However, the TC
50 value of Tet-Sil could not be determined due to its poor solubility. DNA damage was evaluated in the sister chromatid exchange (SCE) assay with CHO cells, and the alkaline comet assay with L929 cells. In contrast to the siloranes, the oxiranes exhibited significant increases (
p
>
0.05) in SCE frequencies and DNA migration relative to their solvent controls. Our findings support previous reports that siloranes have low genotoxic potential and can be suitable components for development of biomaterials.</abstract><cop>Amsterdam</cop><pub>Elsevier B.V</pub><pmid>17719836</pmid><doi>10.1016/j.mrgentox.2007.07.003</doi><tpages>7</tpages></addata></record> |
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| subjects | Animals Biological and medical sciences Cell culture Cell Survival - drug effects CHO Cells Comet Assay Cricetinae Cricetulus DNA damage DNA Damage - drug effects Epoxy Resins - toxicity Ethylene Oxide - analogs & derivatives Ethylene Oxide - chemistry Ethylene Oxide - toxicity Fundamental and applied biological sciences. Psychology Genetics of eukaryotes. Biological and molecular evolution Medical sciences Mice Oxiranes Silicon Silicon Compounds - toxicity Siloranes Sister Chromatid Exchange Sister chromatid exchanges Toxicology |
| title | Assessment of cytotoxicity and DNA damage exhibited by siloranes and oxiranes in cultured mammalian cells |
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