Enhanced production and immunological characterization of recombinant West Nile virus envelope domain III protein

•Envelope domain III is an important target protein for vaccine development against West Nile virus.•Large scale process developed for production of envelope domain III protein.•Two step chromatographic process employed to achieve high level of purity protein.•Vaccine potential of refolded and purif...

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Veröffentlicht in:	New biotechnology 2018-11, Vol.46, p.7-13
Hauptverfasser:	Tripathi, Nagesh K., Karothia, Divyanshi, Shrivastava, Ambuj, Banger, Swati, Kumar, Jyoti S.
Format:	Artikel
Sprache:	eng
Schlagworte:	Affinity Affinity chromatography Antibodies Aquatic insects Batch culture Batch processing Bioreactor Bioreactors Biotechnology Cell culture Chromatography Cultivation E coli Encephalitis Escherichia coli Immunogenicity Immunology Ion exchange Membrane reactors Protein purification Proteins Purification Rabbits Recombinant protein Vaccines Viruses Weight West Nile virus Wet cells
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