Characterization of temperature-sensitive and lipopolysaccharide overproducing transposon mutants of Pseudomonas putida CA-3 affected in PHA accumulation

A library of 20 000 transposon (Tn5) mutants of the gram-negative bacterium Pseudomonas putida CA-3 was generated and screened for adverse affects in polyhydroxyalkanoates (PHA) accumulation. Two mutants of interest were characterized phenotypically. CA-3-126, a mutant disrupted in a stress-related...

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Veröffentlicht in:	FEMS microbiology letters 2009-03, Vol.292 (2), p.297-305
Hauptverfasser:	Goff, Miriam, Nikodinovic-Runic, Jasmina, O'Connor, Kevin E
Format:	Artikel
Sprache:	eng
Schlagworte:	Accumulation Bacterial Proteins - genetics Bacteriology Biological and medical sciences Biomass Carbon - metabolism Carbon sources DNA Transposable Elements Fundamental and applied biological sciences. Psychology Growth conditions High temperature Hot Temperature lipopolysaccharide Lipopolysaccharides Lipopolysaccharides - metabolism Metabolism. Enzymes Microbiology Mutagenesis, Insertional Mutants Nitrogen Nitrogen - metabolism Peptide Hydrolases - genetics Phenylalanine polyhydroxyalkanoate Polyhydroxyalkanoates Polyhydroxyalkanoates - metabolism Pseudomonas putida Pseudomonas putida - genetics Pseudomonas putida - growth & development Pseudomonas putida - metabolism Pseudomonas putida CA-3 Substrates Transaminases - genetics Transaminases - metabolism Transposons
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