Clonogenic assay of cells in vitro

Clonogenic assay or colony formation assay is an in vitro cell survival assay based on the ability of a single cell to grow into a colony. The colony is defined to consist of at least 50 cells. The assay essentially tests every cell in the population for its ability to undergo “unlimited” division....

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Bibliographische Detailangaben
Veröffentlicht in:	Nature protocols 2006-12, Vol.1 (5), p.2315-2319
Hauptverfasser:	Franken, Nicolaas A P, Rodermond, Hans M, Stap, Jan, Haveman, Jaap, van Bree, Chris
Format:	Artikel
Sprache:	eng
Schlagworte:	Analytical Chemistry Assaying Biological assay Biological Techniques Biomedical and Life Sciences Cell Culture Techniques Cell death Cell Proliferation - drug effects Cell Proliferation - radiation effects Cell survival Cells Cloning Colonies Colony-Forming Units Assay - methods Computational Biology/Bioinformatics Cytotoxic agents Cytotoxicity Humans Ionizing radiation Life Sciences Methods Microarrays Organic Chemistry Physiological aspects protocol Radiation Radiation Dosage Stains and staining (Microscopy) Stem cells Survival Tumors X-rays
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