Aptamer-Conjugated Nanoparticles for the Collection and Detection of Multiple Cancer Cells
We have extended the use the aptamer-conjugated nanoparticles for the collection and detection of multiple cancer cells. The aptamers were selected using a cell-based SELEX strategy in our laboratory for cancer cells that, when utilized in this method, allow for the selective recognition of the cell...
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| Veröffentlicht in: | Analytical chemistry (Washington) 2007-04, Vol.79 (8), p.3075-3082 |
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| creator | Smith, Joshua E Medley, Colin D Tang, Zhiwen Shangguan, Dihua Lofton, Charles Tan, Weihong |
| description | We have extended the use the aptamer-conjugated nanoparticles for the collection and detection of multiple cancer cells. The aptamers were selected using a cell-based SELEX strategy in our laboratory for cancer cells that, when utilized in this method, allow for the selective recognition of the cells from complex mixtures including fetal bovine serum samples. Aptamer-conjugated magnetic nanoparticles were used for the selective targeting cell extraction, and aptamer-conjugated fluorescent nanoparticles were employed for sensitive cellular detection. Employing both types of nanoparticles allows for selective and sensitive detection not possible by using the particles separately. Fluorescent nanoparticles amplify the signal intensity versus a single fluorophore label resulting in improved sensitivity. In addition, aptamer-conjugated magnetic nanoparticles allow for extraction and enrichment of target cells not possible with other separation methods. Fluorescent imaging and a microplate reader were used for cellular detection to demonstrate the wide applicability of this methodology for medical diagnostics and cell enrichment and separation. |
| doi_str_mv | 10.1021/ac062151b |
| format | Article |
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The aptamers were selected using a cell-based SELEX strategy in our laboratory for cancer cells that, when utilized in this method, allow for the selective recognition of the cells from complex mixtures including fetal bovine serum samples. Aptamer-conjugated magnetic nanoparticles were used for the selective targeting cell extraction, and aptamer-conjugated fluorescent nanoparticles were employed for sensitive cellular detection. Employing both types of nanoparticles allows for selective and sensitive detection not possible by using the particles separately. Fluorescent nanoparticles amplify the signal intensity versus a single fluorophore label resulting in improved sensitivity. In addition, aptamer-conjugated magnetic nanoparticles allow for extraction and enrichment of target cells not possible with other separation methods. Fluorescent imaging and a microplate reader were used for cellular detection to demonstrate the wide applicability of this methodology for medical diagnostics and cell enrichment and separation.</description><identifier>ISSN: 0003-2700</identifier><identifier>EISSN: 1520-6882</identifier><identifier>DOI: 10.1021/ac062151b</identifier><identifier>PMID: 17348633</identifier><identifier>CODEN: ANCHAM</identifier><language>eng</language><publisher>Washington, DC: American Chemical Society</publisher><subject>Analytical chemistry ; Aptamers, Nucleotide - chemistry ; Burkitt Lymphoma - genetics ; Burkitt Lymphoma - pathology ; Cancer ; Cattle ; Cell Line, Tumor ; Cells ; Chemistry ; Embryos ; Exact sciences and technology ; Fluorescent Dyes - chemistry ; Humans ; Leukemia-Lymphoma, Adult T-Cell - genetics ; Leukemia-Lymphoma, Adult T-Cell - pathology ; Lymphoma, B-Cell - genetics ; Lymphoma, B-Cell - pathology ; Magnetics ; Nanoparticles ; Nanoparticles - chemistry ; SELEX Aptamer Technique - methods ; Spectrometric and optical methods</subject><ispartof>Analytical chemistry (Washington), 2007-04, Vol.79 (8), p.3075-3082</ispartof><rights>Copyright © 2007 American Chemical Society</rights><rights>2007 INIST-CNRS</rights><rights>Copyright American Chemical Society Apr 15, 2007</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-a474t-8fb6d3a1f5bb61bed0b25c6d5bd4fa5111c21eb6e515622d1713170ed4154d153</citedby><cites>FETCH-LOGICAL-a474t-8fb6d3a1f5bb61bed0b25c6d5bd4fa5111c21eb6e515622d1713170ed4154d153</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://pubs.acs.org/doi/pdf/10.1021/ac062151b$$EPDF$$P50$$Gacs$$H</linktopdf><linktohtml>$$Uhttps://pubs.acs.org/doi/10.1021/ac062151b$$EHTML$$P50$$Gacs$$H</linktohtml><link.rule.ids>314,776,780,2752,27053,27901,27902,56713,56763</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=18709455$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/17348633$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Smith, Joshua E</creatorcontrib><creatorcontrib>Medley, Colin D</creatorcontrib><creatorcontrib>Tang, Zhiwen</creatorcontrib><creatorcontrib>Shangguan, Dihua</creatorcontrib><creatorcontrib>Lofton, Charles</creatorcontrib><creatorcontrib>Tan, Weihong</creatorcontrib><title>Aptamer-Conjugated Nanoparticles for the Collection and Detection of Multiple Cancer Cells</title><title>Analytical chemistry (Washington)</title><addtitle>Anal. Chem</addtitle><description>We have extended the use the aptamer-conjugated nanoparticles for the collection and detection of multiple cancer cells. The aptamers were selected using a cell-based SELEX strategy in our laboratory for cancer cells that, when utilized in this method, allow for the selective recognition of the cells from complex mixtures including fetal bovine serum samples. Aptamer-conjugated magnetic nanoparticles were used for the selective targeting cell extraction, and aptamer-conjugated fluorescent nanoparticles were employed for sensitive cellular detection. Employing both types of nanoparticles allows for selective and sensitive detection not possible by using the particles separately. Fluorescent nanoparticles amplify the signal intensity versus a single fluorophore label resulting in improved sensitivity. In addition, aptamer-conjugated magnetic nanoparticles allow for extraction and enrichment of target cells not possible with other separation methods. Fluorescent imaging and a microplate reader were used for cellular detection to demonstrate the wide applicability of this methodology for medical diagnostics and cell enrichment and separation.</description><subject>Analytical chemistry</subject><subject>Aptamers, Nucleotide - chemistry</subject><subject>Burkitt Lymphoma - genetics</subject><subject>Burkitt Lymphoma - pathology</subject><subject>Cancer</subject><subject>Cattle</subject><subject>Cell Line, Tumor</subject><subject>Cells</subject><subject>Chemistry</subject><subject>Embryos</subject><subject>Exact sciences and technology</subject><subject>Fluorescent Dyes - chemistry</subject><subject>Humans</subject><subject>Leukemia-Lymphoma, Adult T-Cell - genetics</subject><subject>Leukemia-Lymphoma, Adult T-Cell - pathology</subject><subject>Lymphoma, B-Cell - genetics</subject><subject>Lymphoma, B-Cell - pathology</subject><subject>Magnetics</subject><subject>Nanoparticles</subject><subject>Nanoparticles - chemistry</subject><subject>SELEX Aptamer Technique - methods</subject><subject>Spectrometric and optical methods</subject><issn>0003-2700</issn><issn>1520-6882</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2007</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpl0F2L1DAUBuAgijuuXvgHpAgKXlRzkibpXi71E1ZdcIRlb0I-TrVjpukmKei_t8uUHdCrcMjDyzkvIU-BvgbK4I1xVDIQYO-RDQhGa9m27D7ZUEp5zRSlJ-RRzjtKASjIh-QEFG9ayfmGXJ9Pxewx1V0cd_MPU9BXX8wYJ5PK4ALmqo-pKj-x6mII6MoQx8qMvnqLZZ1iX32eQxmmsCAzOkxVhyHkx-RBb0LGJ-t7Sr6_f7ftPtYXXz986s4vatOoptRtb6XnBnphrQSLnlomnPTC-qY3AgAcA7QSBQjJmAcFHBRF34BoPAh-Sl4ecqcUb2bMRe-H7JYNzIhxzppR3krW8AU-_wfu4pzGZTfNQLVKNuJsQa8OyKWYc8JeT2nYm_RHA9W3beu7thf7bA2c7R79Ua71LuDFCkx2JvRpqWfIR9cqetaI2xPqgxtywd93_yb90lJxJfT28puW7dWlur7aanbMNS4fj_h_wb9_oqDt</recordid><startdate>20070415</startdate><enddate>20070415</enddate><creator>Smith, Joshua E</creator><creator>Medley, Colin D</creator><creator>Tang, Zhiwen</creator><creator>Shangguan, Dihua</creator><creator>Lofton, Charles</creator><creator>Tan, Weihong</creator><general>American Chemical Society</general><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QF</scope><scope>7QO</scope><scope>7QQ</scope><scope>7SC</scope><scope>7SE</scope><scope>7SP</scope><scope>7SR</scope><scope>7TA</scope><scope>7TB</scope><scope>7TM</scope><scope>7U5</scope><scope>7U7</scope><scope>7U9</scope><scope>8BQ</scope><scope>8FD</scope><scope>C1K</scope><scope>F28</scope><scope>FR3</scope><scope>H8D</scope><scope>H8G</scope><scope>H94</scope><scope>JG9</scope><scope>JQ2</scope><scope>KR7</scope><scope>L7M</scope><scope>L~C</scope><scope>L~D</scope><scope>P64</scope></search><sort><creationdate>20070415</creationdate><title>Aptamer-Conjugated Nanoparticles for the Collection and Detection of Multiple Cancer Cells</title><author>Smith, Joshua E ; Medley, Colin D ; Tang, Zhiwen ; Shangguan, Dihua ; Lofton, Charles ; Tan, Weihong</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-a474t-8fb6d3a1f5bb61bed0b25c6d5bd4fa5111c21eb6e515622d1713170ed4154d153</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2007</creationdate><topic>Analytical chemistry</topic><topic>Aptamers, Nucleotide - chemistry</topic><topic>Burkitt Lymphoma - genetics</topic><topic>Burkitt Lymphoma - pathology</topic><topic>Cancer</topic><topic>Cattle</topic><topic>Cell Line, Tumor</topic><topic>Cells</topic><topic>Chemistry</topic><topic>Embryos</topic><topic>Exact sciences and technology</topic><topic>Fluorescent Dyes - chemistry</topic><topic>Humans</topic><topic>Leukemia-Lymphoma, Adult T-Cell - genetics</topic><topic>Leukemia-Lymphoma, Adult T-Cell - pathology</topic><topic>Lymphoma, B-Cell - genetics</topic><topic>Lymphoma, B-Cell - pathology</topic><topic>Magnetics</topic><topic>Nanoparticles</topic><topic>Nanoparticles - chemistry</topic><topic>SELEX Aptamer Technique - methods</topic><topic>Spectrometric and optical methods</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Smith, Joshua E</creatorcontrib><creatorcontrib>Medley, Colin D</creatorcontrib><creatorcontrib>Tang, Zhiwen</creatorcontrib><creatorcontrib>Shangguan, Dihua</creatorcontrib><creatorcontrib>Lofton, Charles</creatorcontrib><creatorcontrib>Tan, Weihong</creatorcontrib><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Aluminium Industry Abstracts</collection><collection>Biotechnology Research Abstracts</collection><collection>Ceramic Abstracts</collection><collection>Computer and Information Systems Abstracts</collection><collection>Corrosion Abstracts</collection><collection>Electronics & Communications Abstracts</collection><collection>Engineered Materials Abstracts</collection><collection>Materials Business File</collection><collection>Mechanical & Transportation Engineering Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>Toxicology Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>METADEX</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ANTE: Abstracts in New Technology & Engineering</collection><collection>Engineering Research Database</collection><collection>Aerospace Database</collection><collection>Copper Technical Reference Library</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Materials Research Database</collection><collection>ProQuest Computer Science Collection</collection><collection>Civil Engineering Abstracts</collection><collection>Advanced Technologies Database with Aerospace</collection><collection>Computer and Information Systems Abstracts Academic</collection><collection>Computer and Information Systems Abstracts Professional</collection><collection>Biotechnology and BioEngineering Abstracts</collection><jtitle>Analytical chemistry (Washington)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Smith, Joshua E</au><au>Medley, Colin D</au><au>Tang, Zhiwen</au><au>Shangguan, Dihua</au><au>Lofton, Charles</au><au>Tan, Weihong</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Aptamer-Conjugated Nanoparticles for the Collection and Detection of Multiple Cancer Cells</atitle><jtitle>Analytical chemistry (Washington)</jtitle><addtitle>Anal. Chem</addtitle><date>2007-04-15</date><risdate>2007</risdate><volume>79</volume><issue>8</issue><spage>3075</spage><epage>3082</epage><pages>3075-3082</pages><issn>0003-2700</issn><eissn>1520-6882</eissn><coden>ANCHAM</coden><abstract>We have extended the use the aptamer-conjugated nanoparticles for the collection and detection of multiple cancer cells. The aptamers were selected using a cell-based SELEX strategy in our laboratory for cancer cells that, when utilized in this method, allow for the selective recognition of the cells from complex mixtures including fetal bovine serum samples. Aptamer-conjugated magnetic nanoparticles were used for the selective targeting cell extraction, and aptamer-conjugated fluorescent nanoparticles were employed for sensitive cellular detection. Employing both types of nanoparticles allows for selective and sensitive detection not possible by using the particles separately. Fluorescent nanoparticles amplify the signal intensity versus a single fluorophore label resulting in improved sensitivity. In addition, aptamer-conjugated magnetic nanoparticles allow for extraction and enrichment of target cells not possible with other separation methods. Fluorescent imaging and a microplate reader were used for cellular detection to demonstrate the wide applicability of this methodology for medical diagnostics and cell enrichment and separation.</abstract><cop>Washington, DC</cop><pub>American Chemical Society</pub><pmid>17348633</pmid><doi>10.1021/ac062151b</doi><tpages>8</tpages></addata></record> |
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| subjects | Analytical chemistry Aptamers, Nucleotide - chemistry Burkitt Lymphoma - genetics Burkitt Lymphoma - pathology Cancer Cattle Cell Line, Tumor Cells Chemistry Embryos Exact sciences and technology Fluorescent Dyes - chemistry Humans Leukemia-Lymphoma, Adult T-Cell - genetics Leukemia-Lymphoma, Adult T-Cell - pathology Lymphoma, B-Cell - genetics Lymphoma, B-Cell - pathology Magnetics Nanoparticles Nanoparticles - chemistry SELEX Aptamer Technique - methods Spectrometric and optical methods |
| title | Aptamer-Conjugated Nanoparticles for the Collection and Detection of Multiple Cancer Cells |
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