The effect of storage temperature on the biological activity of extracellular vesicles for the complement system
Extracellular vesicles (EVs) are mediators of intercellular communication by transporting cargo containing proteins, lipids, mRNA, and miRNA. There is increasing evidence that EVs have various roles in regulating migration, invasion, stemness, survival, and immune functions. Previously, we have foun...
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| Veröffentlicht in: | In vitro cellular & developmental biology. Animal 2018-06, Vol.54 (6), p.423-429 |
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| creator | Park, Sang June Jeon, Hyungtaek Yoo, Seung-Min Lee, Myung-Shin |
| description | Extracellular vesicles (EVs) are mediators of intercellular communication by transporting cargo containing proteins, lipids, mRNA, and miRNA. There is increasing evidence that EVs have various roles in regulating migration, invasion, stemness, survival, and immune functions. Previously, we have found that EVs from Kaposi's sarcoma-associated herpesvirus (KSHV)-infected human endothelial cells have the potential to activate the complement system. Although many studies have shown that the physical properties of EVs can be changed by their storage condition, there have been few studies for the stability of biological activity of EVs in various storage conditions. In this study, we investigated various conditions to identify the best conditions to store EVs with functional stability for 25 d. Furthermore, the correlation between the function and other characteristics of EVs, including the expression of EV markers, size distribution, and particle number, were also analyzed. Our results demonstrated that storage temperature is an important factor to maintain the activity of EVs and would be useful information for basic research and clinical application using EVs. |
| doi_str_mv | 10.1007/s11626-018-0261-7 |
| format | Article |
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There is increasing evidence that EVs have various roles in regulating migration, invasion, stemness, survival, and immune functions. Previously, we have found that EVs from Kaposi's sarcoma-associated herpesvirus (KSHV)-infected human endothelial cells have the potential to activate the complement system. Although many studies have shown that the physical properties of EVs can be changed by their storage condition, there have been few studies for the stability of biological activity of EVs in various storage conditions. In this study, we investigated various conditions to identify the best conditions to store EVs with functional stability for 25 d. Furthermore, the correlation between the function and other characteristics of EVs, including the expression of EV markers, size distribution, and particle number, were also analyzed. Our results demonstrated that storage temperature is an important factor to maintain the activity of EVs and would be useful information for basic research and clinical application using EVs.</description><identifier>ISSN: 1071-2690</identifier><identifier>EISSN: 1543-706X</identifier><identifier>DOI: 10.1007/s11626-018-0261-7</identifier><identifier>PMID: 29748909</identifier><language>eng</language><publisher>New York: Springer Science & Business Media LLC</publisher><subject>Angiogenesis ; Animal Genetics and Genomics ; Biological activity ; Biomarkers - metabolism ; Biomedical and Life Sciences ; BIOTECHNOLOGY ; Cancer ; Cell Biology ; Cell Culture ; Cell signaling ; Communications systems ; Complement activation ; Developmental Biology ; Drug resistance ; Endothelial cells ; Extracellular vesicles ; Extracellular Vesicles - physiology ; Herpesvirus 8, Human ; Human Umbilical Vein Endothelial Cells ; Humans ; Immunoglobulins ; Infections ; Life Sciences ; Lipids ; Migration ; miRNA ; mRNA ; Nanoparticles ; Particle size distribution ; Physical properties ; Proteomics ; Sarcoma ; Size distribution ; Specimen Handling - methods ; Stability ; Stem Cells ; Storage ; Storage conditions ; Storage temperature ; Studies ; Temperature ; Tetraspanin 28 - metabolism ; Tetraspanin 30 - metabolism ; Time Factors ; Vesicles ; Viral infections ; Viruses</subject><ispartof>In vitro cellular & developmental biology. Animal, 2018-06, Vol.54 (6), p.423-429</ispartof><rights>2018 Society for In Vitro Biology</rights><rights>The Society for In Vitro Biology 2018</rights><rights>Copyright Society for In Vitro Biology Jun 2018</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c460t-54cd2ed728fdf2300d4ef73ed6946021f13abc6f0bb48920d31b85e56febc0e63</citedby><cites>FETCH-LOGICAL-c460t-54cd2ed728fdf2300d4ef73ed6946021f13abc6f0bb48920d31b85e56febc0e63</cites><orcidid>0000-0001-9145-6190</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.jstor.org/stable/pdf/26578105$$EPDF$$P50$$Gjstor$$H</linktopdf><linktohtml>$$Uhttps://www.jstor.org/stable/26578105$$EHTML$$P50$$Gjstor$$H</linktohtml><link.rule.ids>314,780,784,803,27924,27925,41488,42557,51319,58017,58250</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/29748909$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Park, Sang June</creatorcontrib><creatorcontrib>Jeon, Hyungtaek</creatorcontrib><creatorcontrib>Yoo, Seung-Min</creatorcontrib><creatorcontrib>Lee, Myung-Shin</creatorcontrib><title>The effect of storage temperature on the biological activity of extracellular vesicles for the complement system</title><title>In vitro cellular & developmental biology. Animal</title><addtitle>In Vitro Cell.Dev.Biol.-Animal</addtitle><addtitle>In Vitro Cell Dev Biol Anim</addtitle><description>Extracellular vesicles (EVs) are mediators of intercellular communication by transporting cargo containing proteins, lipids, mRNA, and miRNA. There is increasing evidence that EVs have various roles in regulating migration, invasion, stemness, survival, and immune functions. Previously, we have found that EVs from Kaposi's sarcoma-associated herpesvirus (KSHV)-infected human endothelial cells have the potential to activate the complement system. Although many studies have shown that the physical properties of EVs can be changed by their storage condition, there have been few studies for the stability of biological activity of EVs in various storage conditions. In this study, we investigated various conditions to identify the best conditions to store EVs with functional stability for 25 d. Furthermore, the correlation between the function and other characteristics of EVs, including the expression of EV markers, size distribution, and particle number, were also analyzed. Our results demonstrated that storage temperature is an important factor to maintain the activity of EVs and would be useful information for basic research and clinical application using EVs.</description><subject>Angiogenesis</subject><subject>Animal Genetics and Genomics</subject><subject>Biological activity</subject><subject>Biomarkers - metabolism</subject><subject>Biomedical and Life Sciences</subject><subject>BIOTECHNOLOGY</subject><subject>Cancer</subject><subject>Cell Biology</subject><subject>Cell Culture</subject><subject>Cell signaling</subject><subject>Communications systems</subject><subject>Complement activation</subject><subject>Developmental Biology</subject><subject>Drug resistance</subject><subject>Endothelial cells</subject><subject>Extracellular vesicles</subject><subject>Extracellular Vesicles - physiology</subject><subject>Herpesvirus 8, Human</subject><subject>Human Umbilical Vein Endothelial Cells</subject><subject>Humans</subject><subject>Immunoglobulins</subject><subject>Infections</subject><subject>Life Sciences</subject><subject>Lipids</subject><subject>Migration</subject><subject>miRNA</subject><subject>mRNA</subject><subject>Nanoparticles</subject><subject>Particle size distribution</subject><subject>Physical properties</subject><subject>Proteomics</subject><subject>Sarcoma</subject><subject>Size distribution</subject><subject>Specimen Handling - methods</subject><subject>Stability</subject><subject>Stem Cells</subject><subject>Storage</subject><subject>Storage conditions</subject><subject>Storage temperature</subject><subject>Studies</subject><subject>Temperature</subject><subject>Tetraspanin 28 - metabolism</subject><subject>Tetraspanin 30 - metabolism</subject><subject>Time Factors</subject><subject>Vesicles</subject><subject>Viral infections</subject><subject>Viruses</subject><issn>1071-2690</issn><issn>1543-706X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2018</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNp9kcFu1TAQRa0KREvLB3RBZYlNN4GxndjJElVQkCqxKVJ3luOMX_OUxMF2Kt7f45CWSl3gjS3NuXdmfAk5Z_CRAahPkTHJZQGsLoBLVqgjcsKqUhQK5N2r_AbFCi4bOCZvY9xDPg2Tb8gxb1RZN9CckPn2Hik6hzZR72hMPpgd0oTjjMGkJSD1E00Zans_-F1vzUCNTf1Dnw6rAn-nYCwOwzKYQB8w9nbASJ0Pf1XWj_OAI06JxkPMtmfktTNDxHeP9yn5-fXL7dW34ubH9ferzzeFLSWkoiptx7FTvHad4wKgK9EpgZ1scp0zx4RprXTQtnkTDp1gbV1hJR22FlCKU3K5-c7B_1owJj32cZ3TTOiXqDmImkupoMzohxfo3i9hytNpzgSo3J2rTLGNssHHGNDpOfSjCQfNQK9x6C0OnePQaxx61Vw8Oi_tiN0_xdP_Z4BvQMylaYfhufX_XN9vov0a17OprFTNoBJ_ACLToHA</recordid><startdate>20180601</startdate><enddate>20180601</enddate><creator>Park, Sang June</creator><creator>Jeon, Hyungtaek</creator><creator>Yoo, Seung-Min</creator><creator>Lee, Myung-Shin</creator><general>Springer Science & Business Media LLC</general><general>Springer US</general><general>Society for In Vitro Biology</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>4T-</scope><scope>7QL</scope><scope>7T7</scope><scope>7TK</scope><scope>7U9</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>88I</scope><scope>8AF</scope><scope>8AO</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M2P</scope><scope>M7N</scope><scope>M7P</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>Q9U</scope><scope>S0X</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0001-9145-6190</orcidid></search><sort><creationdate>20180601</creationdate><title>The effect of storage temperature on the biological activity of extracellular vesicles for the complement system</title><author>Park, Sang June ; Jeon, Hyungtaek ; Yoo, Seung-Min ; Lee, Myung-Shin</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c460t-54cd2ed728fdf2300d4ef73ed6946021f13abc6f0bb48920d31b85e56febc0e63</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2018</creationdate><topic>Angiogenesis</topic><topic>Animal Genetics and Genomics</topic><topic>Biological activity</topic><topic>Biomarkers - metabolism</topic><topic>Biomedical and Life Sciences</topic><topic>BIOTECHNOLOGY</topic><topic>Cancer</topic><topic>Cell Biology</topic><topic>Cell Culture</topic><topic>Cell signaling</topic><topic>Communications systems</topic><topic>Complement activation</topic><topic>Developmental Biology</topic><topic>Drug resistance</topic><topic>Endothelial cells</topic><topic>Extracellular vesicles</topic><topic>Extracellular Vesicles - physiology</topic><topic>Herpesvirus 8, Human</topic><topic>Human Umbilical Vein Endothelial Cells</topic><topic>Humans</topic><topic>Immunoglobulins</topic><topic>Infections</topic><topic>Life Sciences</topic><topic>Lipids</topic><topic>Migration</topic><topic>miRNA</topic><topic>mRNA</topic><topic>Nanoparticles</topic><topic>Particle size distribution</topic><topic>Physical properties</topic><topic>Proteomics</topic><topic>Sarcoma</topic><topic>Size distribution</topic><topic>Specimen Handling - methods</topic><topic>Stability</topic><topic>Stem Cells</topic><topic>Storage</topic><topic>Storage conditions</topic><topic>Storage temperature</topic><topic>Studies</topic><topic>Temperature</topic><topic>Tetraspanin 28 - 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Academic</collection><jtitle>In vitro cellular & developmental biology. Animal</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Park, Sang June</au><au>Jeon, Hyungtaek</au><au>Yoo, Seung-Min</au><au>Lee, Myung-Shin</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The effect of storage temperature on the biological activity of extracellular vesicles for the complement system</atitle><jtitle>In vitro cellular & developmental biology. Animal</jtitle><stitle>In Vitro Cell.Dev.Biol.-Animal</stitle><addtitle>In Vitro Cell Dev Biol Anim</addtitle><date>2018-06-01</date><risdate>2018</risdate><volume>54</volume><issue>6</issue><spage>423</spage><epage>429</epage><pages>423-429</pages><issn>1071-2690</issn><eissn>1543-706X</eissn><abstract>Extracellular vesicles (EVs) are mediators of intercellular communication by transporting cargo containing proteins, lipids, mRNA, and miRNA. There is increasing evidence that EVs have various roles in regulating migration, invasion, stemness, survival, and immune functions. Previously, we have found that EVs from Kaposi's sarcoma-associated herpesvirus (KSHV)-infected human endothelial cells have the potential to activate the complement system. Although many studies have shown that the physical properties of EVs can be changed by their storage condition, there have been few studies for the stability of biological activity of EVs in various storage conditions. In this study, we investigated various conditions to identify the best conditions to store EVs with functional stability for 25 d. Furthermore, the correlation between the function and other characteristics of EVs, including the expression of EV markers, size distribution, and particle number, were also analyzed. Our results demonstrated that storage temperature is an important factor to maintain the activity of EVs and would be useful information for basic research and clinical application using EVs.</abstract><cop>New York</cop><pub>Springer Science & Business Media LLC</pub><pmid>29748909</pmid><doi>10.1007/s11626-018-0261-7</doi><tpages>7</tpages><orcidid>https://orcid.org/0000-0001-9145-6190</orcidid></addata></record> |
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| ispartof | In vitro cellular & developmental biology. Animal, 2018-06, Vol.54 (6), p.423-429 |
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| subjects | Angiogenesis Animal Genetics and Genomics Biological activity Biomarkers - metabolism Biomedical and Life Sciences BIOTECHNOLOGY Cancer Cell Biology Cell Culture Cell signaling Communications systems Complement activation Developmental Biology Drug resistance Endothelial cells Extracellular vesicles Extracellular Vesicles - physiology Herpesvirus 8, Human Human Umbilical Vein Endothelial Cells Humans Immunoglobulins Infections Life Sciences Lipids Migration miRNA mRNA Nanoparticles Particle size distribution Physical properties Proteomics Sarcoma Size distribution Specimen Handling - methods Stability Stem Cells Storage Storage conditions Storage temperature Studies Temperature Tetraspanin 28 - metabolism Tetraspanin 30 - metabolism Time Factors Vesicles Viral infections Viruses |
| title | The effect of storage temperature on the biological activity of extracellular vesicles for the complement system |
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