Antioxidant activity of enzymatic extracts from the brown seaweed Undaria pinnatifida by electron spin resonance spectroscopy

Enzyme assisted extraction of Undaria pinnatifida was performed using five proteases (Alcalase, Flavourzyme, Neutrase, Trypsin and Protamex) and six carbohydrases (AMG, Dextrozyme, Maltogenase, Promozyme, Viscozyme and Celluclast) in order to acquire extracts rich in antioxidants. Antioxidant activi...

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Veröffentlicht in:Food science & technology 2009-05, Vol.42 (4), p.874-878
Hauptverfasser: Je, Jae-Young, Park, Pyo-Jam, Kim, Eun-Kyung, Park, Jung-Suk, Yoon, Ho-Dong, Kim, Kwang-Rae, Ahn, Chang-Bum
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container_end_page 878
container_issue 4
container_start_page 874
container_title Food science & technology
container_volume 42
creator Je, Jae-Young
Park, Pyo-Jam
Kim, Eun-Kyung
Park, Jung-Suk
Yoon, Ho-Dong
Kim, Kwang-Rae
Ahn, Chang-Bum
description Enzyme assisted extraction of Undaria pinnatifida was performed using five proteases (Alcalase, Flavourzyme, Neutrase, Trypsin and Protamex) and six carbohydrases (AMG, Dextrozyme, Maltogenase, Promozyme, Viscozyme and Celluclast) in order to acquire extracts rich in antioxidants. Antioxidant activity of the extracts was investigated using electron spin resonance (ESR) spectroscopy on 1,1-diphenyl-2-picrylhydrazyl (DPPH), hydroxyl and superoxide radicals. Extracts exhibited strong radical scavenging activity on DPPH and hydroxyl radical, and activity increased with increasing extract concentration. In contrast to DPPH and hydroxyl radical scavenging activity, extracts showed weak scavenging activity on superoxide radical. Extracts exhibited weak radical scavenging activity compared to vitamin C as a reference compound. However, they are still a good source of antioxidant materials that are safe for consumption and show water-soluble properties.
doi_str_mv 10.1016/j.lwt.2008.10.012
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source Elsevier ScienceDirect Journals
subjects Antioxidant
antioxidant activity
antioxidants
Biological and medical sciences
electron paramagnetic resonance spectroscopy
Electron spin resonance
Enzymatic extraction
enzymatic treatment
extraction
Food engineering
Food industries
Free radical
free radical scavengers
free radicals
Fundamental and applied biological sciences. Psychology
General aspects
glycosidases
proteinases
superoxide dismutase
Undaria pinnatifida
title Antioxidant activity of enzymatic extracts from the brown seaweed Undaria pinnatifida by electron spin resonance spectroscopy
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