Protective effects of β-glucan extracted from barley against benzo[a]pyrene-induced DNA damage in hepatic cell HepG2
The aim of the present study was to assess the genotoxic and antigenotoxic effect of β-glucan (BG) extracted from barley. The genotoxicity of BG was tested in the single-cell gel electrophoresis assays (SCGE)/HepG2 test system. Moreover, the protective effects of BG against the genotoxicity of B[a]P...
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| Veröffentlicht in: | Experimental and toxicologic pathology : official journal of the Gesellschaft für Toxikologische Pathologie 2009, Vol.61 (1), p.83-89 |
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| container_title | Experimental and toxicologic pathology : official journal of the Gesellschaft für Toxikologische Pathologie |
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| creator | Angeli, José Pedro F. Ribeiro, Lúcia R. Angeli, José Lourenço F. Mantovani, Mário S. |
| description | The aim of the present study was to assess the genotoxic and antigenotoxic effect of
β-glucan (BG) extracted from barley. The genotoxicity of BG was tested in the single-cell gel electrophoresis assays (SCGE)/HepG2 test system. Moreover, the protective effects of BG against the genotoxicity of B[a]P were studied to delineate its mechanism of antigenotoxicity using four different treatment protocols – pre-treatment, simultaneous simple, simultaneous with pre-incubation, and post-treatment. The results showed that the compound itself was devoid of mutagenic activity at the three lower concentrations studied (1, 5, and 25
μg/mL); however, genotoxic and cytotoxic effects were seen at 100 and 200
μg/mL, respectively. In combination experiments with B[a]P, pronounced inhibition of DNA migration in the SCGE assay was observed in the two simultaneous treatments, and a smaller reduction was observed in the two other treatments. Thus, the data suggest that BG acts through binding to the genotoxic compound or capturing free radicals produced during its activation. However, the protective effects observed with pre-treatment and post-treatment suggest that the BG may be modulating cell metabolism. |
| doi_str_mv | 10.1016/j.etp.2008.05.003 |
| format | Article |
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β-glucan (BG) extracted from barley. The genotoxicity of BG was tested in the single-cell gel electrophoresis assays (SCGE)/HepG2 test system. Moreover, the protective effects of BG against the genotoxicity of B[a]P were studied to delineate its mechanism of antigenotoxicity using four different treatment protocols – pre-treatment, simultaneous simple, simultaneous with pre-incubation, and post-treatment. The results showed that the compound itself was devoid of mutagenic activity at the three lower concentrations studied (1, 5, and 25
μg/mL); however, genotoxic and cytotoxic effects were seen at 100 and 200
μg/mL, respectively. In combination experiments with B[a]P, pronounced inhibition of DNA migration in the SCGE assay was observed in the two simultaneous treatments, and a smaller reduction was observed in the two other treatments. Thus, the data suggest that BG acts through binding to the genotoxic compound or capturing free radicals produced during its activation. However, the protective effects observed with pre-treatment and post-treatment suggest that the BG may be modulating cell metabolism.</description><identifier>ISSN: 0940-2993</identifier><identifier>EISSN: 1618-1433</identifier><identifier>DOI: 10.1016/j.etp.2008.05.003</identifier><identifier>PMID: 18583117</identifier><language>eng</language><publisher>Munich: Elsevier GmbH</publisher><subject>Antigenotoxicity ; Benzo(a)pyrene - toxicity ; beta-Glucans - pharmacology ; Biological and medical sciences ; Cell Line, Tumor ; Comet Assay ; DNA Damage - drug effects ; Hepatocytes - drug effects ; Hepatocytes - pathology ; HepG2 ; Hordeum - chemistry ; Hordeum vulgare ; Humans ; Investigative techniques, diagnostic techniques (general aspects) ; Medical sciences ; Mutagens - toxicity ; Pathology. Cytology. Biochemistry. Spectrometry. Miscellaneous investigative techniques ; Phytotherapy - methods ; Plant Extracts - pharmacology ; β-Glucan</subject><ispartof>Experimental and toxicologic pathology : official journal of the Gesellschaft für Toxikologische Pathologie, 2009, Vol.61 (1), p.83-89</ispartof><rights>2008 Elsevier GmbH</rights><rights>2009 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c444t-5204d0c83c3b562d3f2f56eb3d10360f4377e5cba7304284ae789cba5e5d78763</citedby><cites>FETCH-LOGICAL-c444t-5204d0c83c3b562d3f2f56eb3d10360f4377e5cba7304284ae789cba5e5d78763</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0940299308000869$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,4010,27902,27903,27904,65309</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=21067148$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/18583117$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Angeli, José Pedro F.</creatorcontrib><creatorcontrib>Ribeiro, Lúcia R.</creatorcontrib><creatorcontrib>Angeli, José Lourenço F.</creatorcontrib><creatorcontrib>Mantovani, Mário S.</creatorcontrib><title>Protective effects of β-glucan extracted from barley against benzo[a]pyrene-induced DNA damage in hepatic cell HepG2</title><title>Experimental and toxicologic pathology : official journal of the Gesellschaft für Toxikologische Pathologie</title><addtitle>Exp Toxicol Pathol</addtitle><description>The aim of the present study was to assess the genotoxic and antigenotoxic effect of
β-glucan (BG) extracted from barley. The genotoxicity of BG was tested in the single-cell gel electrophoresis assays (SCGE)/HepG2 test system. Moreover, the protective effects of BG against the genotoxicity of B[a]P were studied to delineate its mechanism of antigenotoxicity using four different treatment protocols – pre-treatment, simultaneous simple, simultaneous with pre-incubation, and post-treatment. The results showed that the compound itself was devoid of mutagenic activity at the three lower concentrations studied (1, 5, and 25
μg/mL); however, genotoxic and cytotoxic effects were seen at 100 and 200
μg/mL, respectively. In combination experiments with B[a]P, pronounced inhibition of DNA migration in the SCGE assay was observed in the two simultaneous treatments, and a smaller reduction was observed in the two other treatments. Thus, the data suggest that BG acts through binding to the genotoxic compound or capturing free radicals produced during its activation. However, the protective effects observed with pre-treatment and post-treatment suggest that the BG may be modulating cell metabolism.</description><subject>Antigenotoxicity</subject><subject>Benzo(a)pyrene - toxicity</subject><subject>beta-Glucans - pharmacology</subject><subject>Biological and medical sciences</subject><subject>Cell Line, Tumor</subject><subject>Comet Assay</subject><subject>DNA Damage - drug effects</subject><subject>Hepatocytes - drug effects</subject><subject>Hepatocytes - pathology</subject><subject>HepG2</subject><subject>Hordeum - chemistry</subject><subject>Hordeum vulgare</subject><subject>Humans</subject><subject>Investigative techniques, diagnostic techniques (general aspects)</subject><subject>Medical sciences</subject><subject>Mutagens - toxicity</subject><subject>Pathology. Cytology. Biochemistry. Spectrometry. Miscellaneous investigative techniques</subject><subject>Phytotherapy - methods</subject><subject>Plant Extracts - pharmacology</subject><subject>β-Glucan</subject><issn>0940-2993</issn><issn>1618-1433</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2009</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkc2KFDEQx4Mo7rj6AF4kF711W_nqdONp2dVdYVEPehIJ6XRlzNBfJt2L42P5ID6TGWbQm3iqKvhVUfx_hDxlUDJg1ctdictccoC6BFUCiHtkwypWF0wKcZ9soJFQ8KYRZ-RRSjsADo1iD8kZq1UtGNMbsn6I04JuCXdI0fvcJTp5-utnse1XZ0eK35do3YId9XEaaGtjj3tqtzaMaaEtjj-mz_bLvI84YhHGbnUZvXp3QTs72C3SMNKvONslOOqw7-kNztf8MXngbZ_wyamek09vXn-8vClu31-_vby4LZyUcikUB9mBq4UTrap4Jzz3qsJWdAxEBV4KrVG51moBktfSoq6bPCpUna51Jc7Ji-PdOU7fVkyLGUI6vGFHnNZkOAjd8Eb-B8irKieWQXYEXZxSiujNHMNg494wMAcpZmeyFHOQYkCZLCXvPDsdX9sBu78bJwsZeH4CbHK299GOLqQ_HGdQaSbrzL06cpgzuwsYTXIBx5x4iNmc6abwjzd-Awjnqmo</recordid><startdate>2009</startdate><enddate>2009</enddate><creator>Angeli, José Pedro F.</creator><creator>Ribeiro, Lúcia R.</creator><creator>Angeli, José Lourenço F.</creator><creator>Mantovani, Mário S.</creator><general>Elsevier GmbH</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TM</scope><scope>7U7</scope><scope>C1K</scope></search><sort><creationdate>2009</creationdate><title>Protective effects of β-glucan extracted from barley against benzo[a]pyrene-induced DNA damage in hepatic cell HepG2</title><author>Angeli, José Pedro F. ; Ribeiro, Lúcia R. ; Angeli, José Lourenço F. ; Mantovani, Mário S.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c444t-5204d0c83c3b562d3f2f56eb3d10360f4377e5cba7304284ae789cba5e5d78763</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2009</creationdate><topic>Antigenotoxicity</topic><topic>Benzo(a)pyrene - toxicity</topic><topic>beta-Glucans - pharmacology</topic><topic>Biological and medical sciences</topic><topic>Cell Line, Tumor</topic><topic>Comet Assay</topic><topic>DNA Damage - drug effects</topic><topic>Hepatocytes - drug effects</topic><topic>Hepatocytes - pathology</topic><topic>HepG2</topic><topic>Hordeum - chemistry</topic><topic>Hordeum vulgare</topic><topic>Humans</topic><topic>Investigative techniques, diagnostic techniques (general aspects)</topic><topic>Medical sciences</topic><topic>Mutagens - toxicity</topic><topic>Pathology. Cytology. Biochemistry. Spectrometry. Miscellaneous investigative techniques</topic><topic>Phytotherapy - methods</topic><topic>Plant Extracts - pharmacology</topic><topic>β-Glucan</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Angeli, José Pedro F.</creatorcontrib><creatorcontrib>Ribeiro, Lúcia R.</creatorcontrib><creatorcontrib>Angeli, José Lourenço F.</creatorcontrib><creatorcontrib>Mantovani, Mário S.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Nucleic Acids Abstracts</collection><collection>Toxicology Abstracts</collection><collection>Environmental Sciences and Pollution Management</collection><jtitle>Experimental and toxicologic pathology : official journal of the Gesellschaft für Toxikologische Pathologie</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Angeli, José Pedro F.</au><au>Ribeiro, Lúcia R.</au><au>Angeli, José Lourenço F.</au><au>Mantovani, Mário S.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Protective effects of β-glucan extracted from barley against benzo[a]pyrene-induced DNA damage in hepatic cell HepG2</atitle><jtitle>Experimental and toxicologic pathology : official journal of the Gesellschaft für Toxikologische Pathologie</jtitle><addtitle>Exp Toxicol Pathol</addtitle><date>2009</date><risdate>2009</risdate><volume>61</volume><issue>1</issue><spage>83</spage><epage>89</epage><pages>83-89</pages><issn>0940-2993</issn><eissn>1618-1433</eissn><abstract>The aim of the present study was to assess the genotoxic and antigenotoxic effect of
β-glucan (BG) extracted from barley. The genotoxicity of BG was tested in the single-cell gel electrophoresis assays (SCGE)/HepG2 test system. Moreover, the protective effects of BG against the genotoxicity of B[a]P were studied to delineate its mechanism of antigenotoxicity using four different treatment protocols – pre-treatment, simultaneous simple, simultaneous with pre-incubation, and post-treatment. The results showed that the compound itself was devoid of mutagenic activity at the three lower concentrations studied (1, 5, and 25
μg/mL); however, genotoxic and cytotoxic effects were seen at 100 and 200
μg/mL, respectively. In combination experiments with B[a]P, pronounced inhibition of DNA migration in the SCGE assay was observed in the two simultaneous treatments, and a smaller reduction was observed in the two other treatments. Thus, the data suggest that BG acts through binding to the genotoxic compound or capturing free radicals produced during its activation. However, the protective effects observed with pre-treatment and post-treatment suggest that the BG may be modulating cell metabolism.</abstract><cop>Munich</cop><pub>Elsevier GmbH</pub><pmid>18583117</pmid><doi>10.1016/j.etp.2008.05.003</doi><tpages>7</tpages></addata></record> |
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| subjects | Antigenotoxicity Benzo(a)pyrene - toxicity beta-Glucans - pharmacology Biological and medical sciences Cell Line, Tumor Comet Assay DNA Damage - drug effects Hepatocytes - drug effects Hepatocytes - pathology HepG2 Hordeum - chemistry Hordeum vulgare Humans Investigative techniques, diagnostic techniques (general aspects) Medical sciences Mutagens - toxicity Pathology. Cytology. Biochemistry. Spectrometry. Miscellaneous investigative techniques Phytotherapy - methods Plant Extracts - pharmacology β-Glucan |
| title | Protective effects of β-glucan extracted from barley against benzo[a]pyrene-induced DNA damage in hepatic cell HepG2 |
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