Screening of Differentiation-Specific Molecular Biomarkers for Colon Cancer
Background/Aims: Owing to the lack of effective molecular markers to evaluate colon cancer differentiation grade, screening of effective molecular markers for the diagnosis and treatment of colon cancer is of great significance. This study is a screening study for molecular markers related to the di...
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| Veröffentlicht in: | Cellular physiology and biochemistry 2018-01, Vol.46 (6), p.2543-2550 |
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| description | Background/Aims: Owing to the lack of effective molecular markers to evaluate colon cancer differentiation grade, screening of effective molecular markers for the diagnosis and treatment of colon cancer is of great significance. This study is a screening study for molecular markers related to the differentiation of colon using the tissue-specific genes of colon. Methods: This study compared the expression profiles of colon cancer at various differentiation grades and screened the down-regulated genes associated with decreased differentiation. IL22RA1 gene was derived from the intersection of obtained gene and colon tissue-specific genes. We used DriverDB and The Human Protein Atlas to analyze the expression level of IL22RA1 in various tissue cells, also used Kaplan-Meier method to analyze the correlation between IL22RA1 and the survival of colon cancer patients, and then used the ROC curve to analyze the specificity and sensitivity of IL22RA1 diagnosis of differentiated colon cancer. Results: We found that IL22RA1 gene expression was progressively down-regulated in high-differentiated, moderate-differentiated, low-differentiated, and undifferentiated colon cancer tissues. Both RNA and protein levels of IL22RA1 were higher in colon tissues and colon cancer tissues than in other normal and cancer tissues. Comparison of IL22RA1 expression in different cancer cells found that IL22RA1 expression was significantly higher in CACO-2 colon cancer cells than in other cancer cells. Survival analysis showed that IL22RA1 gene expression was positively correlated with the overall survival rate of colon cancer patients (P=0.0224). ROC curve analysis revealed that IL22RA1 expression had good specificity and sensitivity to stage II colon cancer. Conclusion: These findings suggest that IL22RA1 serves as a specific molecular marker for the differentiation of colon cancer. |
| doi_str_mv | 10.1159/000489660 |
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This study is a screening study for molecular markers related to the differentiation of colon using the tissue-specific genes of colon. Methods: This study compared the expression profiles of colon cancer at various differentiation grades and screened the down-regulated genes associated with decreased differentiation. IL22RA1 gene was derived from the intersection of obtained gene and colon tissue-specific genes. We used DriverDB and The Human Protein Atlas to analyze the expression level of IL22RA1 in various tissue cells, also used Kaplan-Meier method to analyze the correlation between IL22RA1 and the survival of colon cancer patients, and then used the ROC curve to analyze the specificity and sensitivity of IL22RA1 diagnosis of differentiated colon cancer. Results: We found that IL22RA1 gene expression was progressively down-regulated in high-differentiated, moderate-differentiated, low-differentiated, and undifferentiated colon cancer tissues. Both RNA and protein levels of IL22RA1 were higher in colon tissues and colon cancer tissues than in other normal and cancer tissues. Comparison of IL22RA1 expression in different cancer cells found that IL22RA1 expression was significantly higher in CACO-2 colon cancer cells than in other cancer cells. Survival analysis showed that IL22RA1 gene expression was positively correlated with the overall survival rate of colon cancer patients (P=0.0224). ROC curve analysis revealed that IL22RA1 expression had good specificity and sensitivity to stage II colon cancer. Conclusion: These findings suggest that IL22RA1 serves as a specific molecular marker for the differentiation of colon cancer.</description><identifier>ISSN: 1015-8987</identifier><identifier>EISSN: 1421-9778</identifier><identifier>DOI: 10.1159/000489660</identifier><identifier>PMID: 29742509</identifier><language>eng</language><publisher>Basel, Switzerland: S. Karger AG</publisher><subject>Bioinformatics ; Biomarkers ; Biomarkers, Tumor - analysis ; Biomarkers, Tumor - genetics ; Caco-2 Cells ; Cell cycle ; Cell Differentiation ; Colon - metabolism ; Colon - pathology ; Colon cancer ; Colonic Neoplasms - diagnosis ; Colonic Neoplasms - genetics ; Colonic Neoplasms - pathology ; Colorectal cancer ; Datasets ; Differentiation grade ; Down-Regulation ; Gene expression ; Gene Expression Regulation, Neoplastic ; Genomes ; Humans ; Lymphatic system ; Medical prognosis ; Metastasis ; Molecular marker ; Original Paper ; Proteins ; Receptors, Interleukin - analysis ; Receptors, Interleukin - genetics ; Survival Analysis ; Tumorigenesis ; Tumors ; Variance analysis</subject><ispartof>Cellular physiology and biochemistry, 2018-01, Vol.46 (6), p.2543-2550</ispartof><rights>2018 The Author(s). Published by S. Karger AG, Basel</rights><rights>2018 The Author(s). Published by S. Karger AG, Basel.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c463t-f29f1ce7ed354c20b8ca4f1774bfa8a728eb18a40569b4a4ec70c422df4bd39d3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>315,781,785,865,2103,27639,27928,27929</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/29742509$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Qi, Lu</creatorcontrib><creatorcontrib>Ding, Yanqing</creatorcontrib><title>Screening of Differentiation-Specific Molecular Biomarkers for Colon Cancer</title><title>Cellular physiology and biochemistry</title><addtitle>Cell Physiol Biochem</addtitle><description>Background/Aims: Owing to the lack of effective molecular markers to evaluate colon cancer differentiation grade, screening of effective molecular markers for the diagnosis and treatment of colon cancer is of great significance. This study is a screening study for molecular markers related to the differentiation of colon using the tissue-specific genes of colon. Methods: This study compared the expression profiles of colon cancer at various differentiation grades and screened the down-regulated genes associated with decreased differentiation. IL22RA1 gene was derived from the intersection of obtained gene and colon tissue-specific genes. We used DriverDB and The Human Protein Atlas to analyze the expression level of IL22RA1 in various tissue cells, also used Kaplan-Meier method to analyze the correlation between IL22RA1 and the survival of colon cancer patients, and then used the ROC curve to analyze the specificity and sensitivity of IL22RA1 diagnosis of differentiated colon cancer. Results: We found that IL22RA1 gene expression was progressively down-regulated in high-differentiated, moderate-differentiated, low-differentiated, and undifferentiated colon cancer tissues. Both RNA and protein levels of IL22RA1 were higher in colon tissues and colon cancer tissues than in other normal and cancer tissues. Comparison of IL22RA1 expression in different cancer cells found that IL22RA1 expression was significantly higher in CACO-2 colon cancer cells than in other cancer cells. Survival analysis showed that IL22RA1 gene expression was positively correlated with the overall survival rate of colon cancer patients (P=0.0224). ROC curve analysis revealed that IL22RA1 expression had good specificity and sensitivity to stage II colon cancer. Conclusion: These findings suggest that IL22RA1 serves as a specific molecular marker for the differentiation of colon cancer.</description><subject>Bioinformatics</subject><subject>Biomarkers</subject><subject>Biomarkers, Tumor - analysis</subject><subject>Biomarkers, Tumor - genetics</subject><subject>Caco-2 Cells</subject><subject>Cell cycle</subject><subject>Cell Differentiation</subject><subject>Colon - metabolism</subject><subject>Colon - pathology</subject><subject>Colon cancer</subject><subject>Colonic Neoplasms - diagnosis</subject><subject>Colonic Neoplasms - genetics</subject><subject>Colonic Neoplasms - pathology</subject><subject>Colorectal cancer</subject><subject>Datasets</subject><subject>Differentiation grade</subject><subject>Down-Regulation</subject><subject>Gene expression</subject><subject>Gene Expression Regulation, Neoplastic</subject><subject>Genomes</subject><subject>Humans</subject><subject>Lymphatic system</subject><subject>Medical prognosis</subject><subject>Metastasis</subject><subject>Molecular marker</subject><subject>Original Paper</subject><subject>Proteins</subject><subject>Receptors, Interleukin - analysis</subject><subject>Receptors, Interleukin - genetics</subject><subject>Survival Analysis</subject><subject>Tumorigenesis</subject><subject>Tumors</subject><subject>Variance analysis</subject><issn>1015-8987</issn><issn>1421-9778</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2018</creationdate><recordtype>article</recordtype><sourceid>M--</sourceid><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DOA</sourceid><recordid>eNptkU1v1DAQhi0EoqVw4I5QJC5wCIwdJ7aPNHxVFIFUOFsTZ7zyNhsvdnLg32PYZZEQJ49Hj56Z0cvYYw4vOW_NKwCQ2nQd3GHnXApeG6X03VIDb2tttDpjD3LeQvkqI-6zM2GUFC2Yc_bxxiWiOcybKvrqTfCeEs1LwCXEub7Zkws-uOpTnMitE6bqMsQdpltKufIxVX2c4lz1ODtKD9k9j1OmR8f3gn179_Zr_6G-_vz-qn99XTvZNUvthfHckaKxaaUTMGiH0nOl5OBRoxKaBq5RQtuZQaIkp8BJIUYvh7ExY3PBrg7eMeLW7lMoC_2wEYP93YhpYzEtwU1kB4KGNGjUTdGLDgdPnjopoCVqsCuu5wfXPsXvK-XF7kJ2NE04U1yzFdAo6LjQoqDP_kG3cU1zudQKzhVXIJu2UC8OlEsx50T-tCAH-yste0qrsE-PxnXY0Xgi_8Tzd-Qtpg2lE9B_uTwo7H70hXryX-o45SeReKL5</recordid><startdate>20180101</startdate><enddate>20180101</enddate><creator>Qi, Lu</creator><creator>Ding, Yanqing</creator><general>S. Karger AG</general><general>Cell Physiol Biochem Press GmbH & Co KG</general><scope>M--</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>BENPR</scope><scope>CCPQU</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>K9.</scope><scope>M0S</scope><scope>M1P</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>7X8</scope><scope>DOA</scope></search><sort><creationdate>20180101</creationdate><title>Screening of Differentiation-Specific Molecular Biomarkers for Colon Cancer</title><author>Qi, Lu ; Ding, Yanqing</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c463t-f29f1ce7ed354c20b8ca4f1774bfa8a728eb18a40569b4a4ec70c422df4bd39d3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2018</creationdate><topic>Bioinformatics</topic><topic>Biomarkers</topic><topic>Biomarkers, Tumor - analysis</topic><topic>Biomarkers, Tumor - genetics</topic><topic>Caco-2 Cells</topic><topic>Cell cycle</topic><topic>Cell Differentiation</topic><topic>Colon - metabolism</topic><topic>Colon - pathology</topic><topic>Colon cancer</topic><topic>Colonic Neoplasms - diagnosis</topic><topic>Colonic Neoplasms - genetics</topic><topic>Colonic Neoplasms - pathology</topic><topic>Colorectal cancer</topic><topic>Datasets</topic><topic>Differentiation grade</topic><topic>Down-Regulation</topic><topic>Gene expression</topic><topic>Gene Expression Regulation, Neoplastic</topic><topic>Genomes</topic><topic>Humans</topic><topic>Lymphatic system</topic><topic>Medical prognosis</topic><topic>Metastasis</topic><topic>Molecular marker</topic><topic>Original Paper</topic><topic>Proteins</topic><topic>Receptors, Interleukin - analysis</topic><topic>Receptors, Interleukin - genetics</topic><topic>Survival Analysis</topic><topic>Tumorigenesis</topic><topic>Tumors</topic><topic>Variance analysis</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Qi, Lu</creatorcontrib><creatorcontrib>Ding, Yanqing</creatorcontrib><collection>Karger Open Access</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>Proquest Central</collection><collection>ProQuest One Community College</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>MEDLINE - Academic</collection><collection>DOAJ Directory of Open Access Journals</collection><jtitle>Cellular physiology and biochemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Qi, Lu</au><au>Ding, Yanqing</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Screening of Differentiation-Specific Molecular Biomarkers for Colon Cancer</atitle><jtitle>Cellular physiology and biochemistry</jtitle><addtitle>Cell Physiol Biochem</addtitle><date>2018-01-01</date><risdate>2018</risdate><volume>46</volume><issue>6</issue><spage>2543</spage><epage>2550</epage><pages>2543-2550</pages><issn>1015-8987</issn><eissn>1421-9778</eissn><abstract>Background/Aims: Owing to the lack of effective molecular markers to evaluate colon cancer differentiation grade, screening of effective molecular markers for the diagnosis and treatment of colon cancer is of great significance. This study is a screening study for molecular markers related to the differentiation of colon using the tissue-specific genes of colon. Methods: This study compared the expression profiles of colon cancer at various differentiation grades and screened the down-regulated genes associated with decreased differentiation. IL22RA1 gene was derived from the intersection of obtained gene and colon tissue-specific genes. We used DriverDB and The Human Protein Atlas to analyze the expression level of IL22RA1 in various tissue cells, also used Kaplan-Meier method to analyze the correlation between IL22RA1 and the survival of colon cancer patients, and then used the ROC curve to analyze the specificity and sensitivity of IL22RA1 diagnosis of differentiated colon cancer. Results: We found that IL22RA1 gene expression was progressively down-regulated in high-differentiated, moderate-differentiated, low-differentiated, and undifferentiated colon cancer tissues. Both RNA and protein levels of IL22RA1 were higher in colon tissues and colon cancer tissues than in other normal and cancer tissues. Comparison of IL22RA1 expression in different cancer cells found that IL22RA1 expression was significantly higher in CACO-2 colon cancer cells than in other cancer cells. Survival analysis showed that IL22RA1 gene expression was positively correlated with the overall survival rate of colon cancer patients (P=0.0224). ROC curve analysis revealed that IL22RA1 expression had good specificity and sensitivity to stage II colon cancer. Conclusion: These findings suggest that IL22RA1 serves as a specific molecular marker for the differentiation of colon cancer.</abstract><cop>Basel, Switzerland</cop><pub>S. Karger AG</pub><pmid>29742509</pmid><doi>10.1159/000489660</doi><tpages>8</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Bioinformatics Biomarkers Biomarkers, Tumor - analysis Biomarkers, Tumor - genetics Caco-2 Cells Cell cycle Cell Differentiation Colon - metabolism Colon - pathology Colon cancer Colonic Neoplasms - diagnosis Colonic Neoplasms - genetics Colonic Neoplasms - pathology Colorectal cancer Datasets Differentiation grade Down-Regulation Gene expression Gene Expression Regulation, Neoplastic Genomes Humans Lymphatic system Medical prognosis Metastasis Molecular marker Original Paper Proteins Receptors, Interleukin - analysis Receptors, Interleukin - genetics Survival Analysis Tumorigenesis Tumors Variance analysis |
| title | Screening of Differentiation-Specific Molecular Biomarkers for Colon Cancer |
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