Rapid Electrochemical Assessment of Tumor Suppressor Gene Methylations in Raw Human Serum and Tumor Cells and Tissues Using Immunomagnetic Beads and Selective DNA Hybridization

We report a rapid and sensitive electrochemical strategy for the detection of gene‐specific 5‐methylcytosine DNA methylation. Magnetic beads (MBs) modified with an antibody for 5‐methylcytosines (5‐mC) are used for the capture of any 5‐mC methylated single‐stranded (ss)DNA sequence. A flanking regio...

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Veröffentlicht in:	Angewandte Chemie International Edition 2018-07, Vol.57 (27), p.8194-8198
Hauptverfasser:	Povedano, Eloy, Valverde, Alejandro, Montiel, Víctor Ruiz‐Valdepeñas, Pedrero, María, Yáñez‐Sedeño, Paloma, Barderas, Rodrigo, San Segundo‐Acosta, Pablo, Peláez‐García, Alberto, Mendiola, Marta, Hardisson, David, Campuzano, Susana, Pingarrón, José M.
Format:	Artikel
Sprache:	eng
Schlagworte:	Beads biofluids Biosensors Brain brain tissues Brain tumors Deoxyribonucleic acid DNA DNA methylation DNA methyltransferase dual detection Electrical measurement Electrochemistry Glioblastoma Glioblastoma cells Hybridization immuno-DNA sensors methylated DNA Methylguanine Nucleotide sequence O6-methylguanine-DNA methyltransferase Paraffin Pretreatment Tissues Tumor cells Tumor suppressor genes Tumors
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