Nociceptive and inflammatory responses induced by formalin in the orofacial region of rats: Effect of anti-TNFα strategies

This study evaluated the effects of different anti-TNFα strategies on the nociceptive and inflammatory responses triggered by formalin in the rat orofacial region. Formalin injection (2.5%) into the right upper lip caused a nociceptive response that was biphasic, with the first phase observed betwee...

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Veröffentlicht in:International immunopharmacology 2009, Vol.9 (1), p.80-85
Hauptverfasser: Seadi Pereira, Paula Juliana, Noronha Dornelles, Fabiana, Santiago Santos, Diógenes, Batista Calixto, João, Bueno Morrone, Fernanda, Campos, Maria Martha
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container_end_page 85
container_issue 1
container_start_page 80
container_title International immunopharmacology
container_volume 9
creator Seadi Pereira, Paula Juliana
Noronha Dornelles, Fabiana
Santiago Santos, Diógenes
Batista Calixto, João
Bueno Morrone, Fernanda
Campos, Maria Martha
description This study evaluated the effects of different anti-TNFα strategies on the nociceptive and inflammatory responses triggered by formalin in the rat orofacial region. Formalin injection (2.5%) into the right upper lip caused a nociceptive response that was biphasic, with the first phase observed between 0 and 3 min and the second phase between 12 and 30 min. Plasma extravasation induced by formalin was time-related and reached the peak at 360 min. The monoclonal antibody anti-TNFα (25 and 50 pg/lip) significantly inhibited the second phase of formalin-induced nociceptive behavior, while the first phase remained unaltered. The systemic treatment with the chimeric anti-TNFα antibody infliximab also caused a significant inhibition of the second phase. Interestingly, the local administration of infliximab (50 pg/lip) produced a significant reduction of both phases of formalin-induced nociception. In addition, the systemic pretreatment with the preferential inhibitor of TNFα synthesis thalidomide (25 and 50 mg/kg, p.o) promoted a marked reduction of the first and second phases of formalin-evoked nociception. The local administration of the monoclonal antibody anti-TNFα (25 and 50 pg/lip) or infliximab (50 pg/lip) markedly reduced the plasma extravasation induced by formalin. Otherwise, formalin-elicited plasma extravasation was not significantly affected by the systemic administration of either infliximab (1 mg/kg; s.c) or thalidomide (50 mg/kg, p.o). Present data suggest that blocking TNFα effects, through different pharmacological tools, could represent a good alternative to control orofacial inflammatory pain that is refractory to other drugs.
doi_str_mv 10.1016/j.intimp.2008.10.001
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The local administration of the monoclonal antibody anti-TNFα (25 and 50 pg/lip) or infliximab (50 pg/lip) markedly reduced the plasma extravasation induced by formalin. Otherwise, formalin-elicited plasma extravasation was not significantly affected by the systemic administration of either infliximab (1 mg/kg; s.c) or thalidomide (50 mg/kg, p.o). Present data suggest that blocking TNFα effects, through different pharmacological tools, could represent a good alternative to control orofacial inflammatory pain that is refractory to other drugs.</description><subject>Biological and medical sciences</subject><subject>Formalin</subject><subject>Medical sciences</subject><subject>Nociception</subject><subject>Orofacial region</subject><subject>Pharmacology. 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subjects Biological and medical sciences
Formalin
Medical sciences
Nociception
Orofacial region
Pharmacology. Drug treatments
Plasma extravasation
Rat
TNFα
title Nociceptive and inflammatory responses induced by formalin in the orofacial region of rats: Effect of anti-TNFα strategies
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