Nociceptive and inflammatory responses induced by formalin in the orofacial region of rats: Effect of anti-TNFα strategies
This study evaluated the effects of different anti-TNFα strategies on the nociceptive and inflammatory responses triggered by formalin in the rat orofacial region. Formalin injection (2.5%) into the right upper lip caused a nociceptive response that was biphasic, with the first phase observed betwee...
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Veröffentlicht in: | International immunopharmacology 2009, Vol.9 (1), p.80-85 |
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description | This study evaluated the effects of different anti-TNFα strategies on the nociceptive and inflammatory responses triggered by formalin in the rat orofacial region. Formalin injection (2.5%) into the right upper lip caused a nociceptive response that was biphasic, with the first phase observed between 0 and 3 min and the second phase between 12 and 30 min. Plasma extravasation induced by formalin was time-related and reached the peak at 360 min. The monoclonal antibody anti-TNFα (25 and 50 pg/lip) significantly inhibited the second phase of formalin-induced nociceptive behavior, while the first phase remained unaltered. The systemic treatment with the chimeric anti-TNFα antibody infliximab also caused a significant inhibition of the second phase. Interestingly, the local administration of infliximab (50 pg/lip) produced a significant reduction of both phases of formalin-induced nociception. In addition, the systemic pretreatment with the preferential inhibitor of TNFα synthesis thalidomide (25 and 50 mg/kg, p.o) promoted a marked reduction of the first and second phases of formalin-evoked nociception. The local administration of the monoclonal antibody anti-TNFα (25 and 50 pg/lip) or infliximab (50 pg/lip) markedly reduced the plasma extravasation induced by formalin. Otherwise, formalin-elicited plasma extravasation was not significantly affected by the systemic administration of either infliximab (1 mg/kg; s.c) or thalidomide (50 mg/kg, p.o). Present data suggest that blocking TNFα effects, through different pharmacological tools, could represent a good alternative to control orofacial inflammatory pain that is refractory to other drugs. |
doi_str_mv | 10.1016/j.intimp.2008.10.001 |
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Formalin injection (2.5%) into the right upper lip caused a nociceptive response that was biphasic, with the first phase observed between 0 and 3 min and the second phase between 12 and 30 min. Plasma extravasation induced by formalin was time-related and reached the peak at 360 min. The monoclonal antibody anti-TNFα (25 and 50 pg/lip) significantly inhibited the second phase of formalin-induced nociceptive behavior, while the first phase remained unaltered. The systemic treatment with the chimeric anti-TNFα antibody infliximab also caused a significant inhibition of the second phase. Interestingly, the local administration of infliximab (50 pg/lip) produced a significant reduction of both phases of formalin-induced nociception. In addition, the systemic pretreatment with the preferential inhibitor of TNFα synthesis thalidomide (25 and 50 mg/kg, p.o) promoted a marked reduction of the first and second phases of formalin-evoked nociception. The local administration of the monoclonal antibody anti-TNFα (25 and 50 pg/lip) or infliximab (50 pg/lip) markedly reduced the plasma extravasation induced by formalin. Otherwise, formalin-elicited plasma extravasation was not significantly affected by the systemic administration of either infliximab (1 mg/kg; s.c) or thalidomide (50 mg/kg, p.o). Present data suggest that blocking TNFα effects, through different pharmacological tools, could represent a good alternative to control orofacial inflammatory pain that is refractory to other drugs.</description><identifier>ISSN: 1567-5769</identifier><identifier>EISSN: 1878-1705</identifier><identifier>DOI: 10.1016/j.intimp.2008.10.001</identifier><language>eng</language><publisher>Kidlington: Elsevier B.V</publisher><subject>Biological and medical sciences ; Formalin ; Medical sciences ; Nociception ; Orofacial region ; Pharmacology. Drug treatments ; Plasma extravasation ; Rat ; TNFα</subject><ispartof>International immunopharmacology, 2009, Vol.9 (1), p.80-85</ispartof><rights>2008 Elsevier B.V.</rights><rights>2009 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c367t-c37eb46b368fd4d0f6556e4b1c9404613ab428b288f2373caa401966b795326d3</citedby><cites>FETCH-LOGICAL-c367t-c37eb46b368fd4d0f6556e4b1c9404613ab428b288f2373caa401966b795326d3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.intimp.2008.10.001$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,4024,27923,27924,27925,45995</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=21071795$$DView record in Pascal Francis$$Hfree_for_read</backlink></links><search><creatorcontrib>Seadi Pereira, Paula Juliana</creatorcontrib><creatorcontrib>Noronha Dornelles, Fabiana</creatorcontrib><creatorcontrib>Santiago Santos, Diógenes</creatorcontrib><creatorcontrib>Batista Calixto, João</creatorcontrib><creatorcontrib>Bueno Morrone, Fernanda</creatorcontrib><creatorcontrib>Campos, Maria Martha</creatorcontrib><title>Nociceptive and inflammatory responses induced by formalin in the orofacial region of rats: Effect of anti-TNFα strategies</title><title>International immunopharmacology</title><description>This study evaluated the effects of different anti-TNFα strategies on the nociceptive and inflammatory responses triggered by formalin in the rat orofacial region. Formalin injection (2.5%) into the right upper lip caused a nociceptive response that was biphasic, with the first phase observed between 0 and 3 min and the second phase between 12 and 30 min. Plasma extravasation induced by formalin was time-related and reached the peak at 360 min. The monoclonal antibody anti-TNFα (25 and 50 pg/lip) significantly inhibited the second phase of formalin-induced nociceptive behavior, while the first phase remained unaltered. The systemic treatment with the chimeric anti-TNFα antibody infliximab also caused a significant inhibition of the second phase. Interestingly, the local administration of infliximab (50 pg/lip) produced a significant reduction of both phases of formalin-induced nociception. In addition, the systemic pretreatment with the preferential inhibitor of TNFα synthesis thalidomide (25 and 50 mg/kg, p.o) promoted a marked reduction of the first and second phases of formalin-evoked nociception. The local administration of the monoclonal antibody anti-TNFα (25 and 50 pg/lip) or infliximab (50 pg/lip) markedly reduced the plasma extravasation induced by formalin. Otherwise, formalin-elicited plasma extravasation was not significantly affected by the systemic administration of either infliximab (1 mg/kg; s.c) or thalidomide (50 mg/kg, p.o). Present data suggest that blocking TNFα effects, through different pharmacological tools, could represent a good alternative to control orofacial inflammatory pain that is refractory to other drugs.</description><subject>Biological and medical sciences</subject><subject>Formalin</subject><subject>Medical sciences</subject><subject>Nociception</subject><subject>Orofacial region</subject><subject>Pharmacology. Drug treatments</subject><subject>Plasma extravasation</subject><subject>Rat</subject><subject>TNFα</subject><issn>1567-5769</issn><issn>1878-1705</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2009</creationdate><recordtype>article</recordtype><recordid>eNp9kM1O3DAURi1EJWDaN-jCm3aXqZ04doZFJYSAIiG6oWvLca6pR4kdfD1Ioz5VX4RnwtEglmz88_nca_sQ8pWzNWdc_tiufch-mtc1Y12J1ozxI3LKO9VVXLH2uKxbqapWyc0JOUPcFkAxwU_Jv_tovYU5-2egJgzUBzeaaTI5pj1NgHMMCFjiYWdhoP2eupgmM_pQMpr_Ao0pOmO9GQv-6GOg0dFkMp7TK-fA5mVvyvuqh_vrl_8UczksIOBn8smZEeHL27wif66vHi5_VXe_b24vL-4q20iVy6igF7JvZOcGMTAn21aC6LndCCYkb0wv6q6vu87VjWqsMYLxjZS92rRNLYdmRb4f-s4pPu0As548WhhHEyDuUNesUYuPAooDaFNETOD0nPxk0l5zphfTeqsPpvViekmLyFL27a2_QWtGl0ywHt9ra84UX96yIj8PHJTPPntIGq2HULT6VDzpIfqPL3oFUQiX8w</recordid><startdate>2009</startdate><enddate>2009</enddate><creator>Seadi Pereira, Paula Juliana</creator><creator>Noronha Dornelles, Fabiana</creator><creator>Santiago Santos, Diógenes</creator><creator>Batista Calixto, João</creator><creator>Bueno Morrone, Fernanda</creator><creator>Campos, Maria Martha</creator><general>Elsevier B.V</general><general>Elsevier</general><scope>IQODW</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7T5</scope><scope>H94</scope></search><sort><creationdate>2009</creationdate><title>Nociceptive and inflammatory responses induced by formalin in the orofacial region of rats: Effect of anti-TNFα strategies</title><author>Seadi Pereira, Paula Juliana ; Noronha Dornelles, Fabiana ; Santiago Santos, Diógenes ; Batista Calixto, João ; Bueno Morrone, Fernanda ; Campos, Maria Martha</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c367t-c37eb46b368fd4d0f6556e4b1c9404613ab428b288f2373caa401966b795326d3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2009</creationdate><topic>Biological and medical sciences</topic><topic>Formalin</topic><topic>Medical sciences</topic><topic>Nociception</topic><topic>Orofacial region</topic><topic>Pharmacology. Drug treatments</topic><topic>Plasma extravasation</topic><topic>Rat</topic><topic>TNFα</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Seadi Pereira, Paula Juliana</creatorcontrib><creatorcontrib>Noronha Dornelles, Fabiana</creatorcontrib><creatorcontrib>Santiago Santos, Diógenes</creatorcontrib><creatorcontrib>Batista Calixto, João</creatorcontrib><creatorcontrib>Bueno Morrone, Fernanda</creatorcontrib><creatorcontrib>Campos, Maria Martha</creatorcontrib><collection>Pascal-Francis</collection><collection>CrossRef</collection><collection>Immunology Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><jtitle>International immunopharmacology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Seadi Pereira, Paula Juliana</au><au>Noronha Dornelles, Fabiana</au><au>Santiago Santos, Diógenes</au><au>Batista Calixto, João</au><au>Bueno Morrone, Fernanda</au><au>Campos, Maria Martha</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Nociceptive and inflammatory responses induced by formalin in the orofacial region of rats: Effect of anti-TNFα strategies</atitle><jtitle>International immunopharmacology</jtitle><date>2009</date><risdate>2009</risdate><volume>9</volume><issue>1</issue><spage>80</spage><epage>85</epage><pages>80-85</pages><issn>1567-5769</issn><eissn>1878-1705</eissn><abstract>This study evaluated the effects of different anti-TNFα strategies on the nociceptive and inflammatory responses triggered by formalin in the rat orofacial region. Formalin injection (2.5%) into the right upper lip caused a nociceptive response that was biphasic, with the first phase observed between 0 and 3 min and the second phase between 12 and 30 min. Plasma extravasation induced by formalin was time-related and reached the peak at 360 min. The monoclonal antibody anti-TNFα (25 and 50 pg/lip) significantly inhibited the second phase of formalin-induced nociceptive behavior, while the first phase remained unaltered. The systemic treatment with the chimeric anti-TNFα antibody infliximab also caused a significant inhibition of the second phase. Interestingly, the local administration of infliximab (50 pg/lip) produced a significant reduction of both phases of formalin-induced nociception. In addition, the systemic pretreatment with the preferential inhibitor of TNFα synthesis thalidomide (25 and 50 mg/kg, p.o) promoted a marked reduction of the first and second phases of formalin-evoked nociception. The local administration of the monoclonal antibody anti-TNFα (25 and 50 pg/lip) or infliximab (50 pg/lip) markedly reduced the plasma extravasation induced by formalin. Otherwise, formalin-elicited plasma extravasation was not significantly affected by the systemic administration of either infliximab (1 mg/kg; s.c) or thalidomide (50 mg/kg, p.o). Present data suggest that blocking TNFα effects, through different pharmacological tools, could represent a good alternative to control orofacial inflammatory pain that is refractory to other drugs.</abstract><cop>Kidlington</cop><pub>Elsevier B.V</pub><doi>10.1016/j.intimp.2008.10.001</doi><tpages>6</tpages></addata></record> |
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subjects | Biological and medical sciences Formalin Medical sciences Nociception Orofacial region Pharmacology. Drug treatments Plasma extravasation Rat TNFα |
title | Nociceptive and inflammatory responses induced by formalin in the orofacial region of rats: Effect of anti-TNFα strategies |
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