Development and validation of an HPLC‐UV method for quantification of elvitegravir and two other new antiretrovirals, dolutegravir and rilpivirine, in the plasma of HIV‐positive patients
Therapeutic drug monitoring may be crucial in selected clinical conditions for the management of HIV infection. In recent years, new antiretrovirals have been introduced and in particular elvitegravir (EVG) is now recommended for first‐line and simplification treatment as well as dolutegravir (DTG)...
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| Veröffentlicht in: | Biomedical chromatography 2018-09, Vol.32 (9), p.e4274-n/a |
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| container_title | Biomedical chromatography |
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| creator | Tempestilli, Massimo Ammassari, Adriana D'Avolio, Antonio Cicalini, Stefania Gallo, Anna Loredana Fazio, Simone Antinori, Andrea Pucillo, Leopoldo P. |
| description | Therapeutic drug monitoring may be crucial in selected clinical conditions for the management of HIV infection. In recent years, new antiretrovirals have been introduced and in particular elvitegravir (EVG) is now recommended for first‐line and simplification treatment as well as dolutegravir (DTG) and rilpivirine (RPV). The aim of this study was to develop and validate a high‐performance liquid chromatography–ultraviolet (HPLC‐UV) method for determining EVG and new antiretrovirals DTG and RPV in human plasma. Solid‐phase extraction was applied to a 600 μL plasma sample. Chromatographic separation of the three drugs and internal standard was achieved with a gradient of acetonitrile and phosphate buffer on a C18 reverse‐phase analytical column with a 20 min analytical run time. EVG and DTG were detected at 265 nm and RPV at 290 nm. Mean intra‐ and inter‐day precisions were |
| doi_str_mv | 10.1002/bmc.4274 |
| format | Article |
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In recent years, new antiretrovirals have been introduced and in particular elvitegravir (EVG) is now recommended for first‐line and simplification treatment as well as dolutegravir (DTG) and rilpivirine (RPV). The aim of this study was to develop and validate a high‐performance liquid chromatography–ultraviolet (HPLC‐UV) method for determining EVG and new antiretrovirals DTG and RPV in human plasma. Solid‐phase extraction was applied to a 600 μL plasma sample. Chromatographic separation of the three drugs and internal standard was achieved with a gradient of acetonitrile and phosphate buffer on a C18 reverse‐phase analytical column with a 20 min analytical run time. EVG and DTG were detected at 265 nm and RPV at 290 nm. Mean intra‐ and inter‐day precisions were < 10%; the mean accuracy was <15%. Extraction recovery ranged between 105 and 82% for the drugs analyzed. Calibration curves were optimized according to the expected ranges of drug concentrations in patients; the coefficient of determination was >0.997 for all drugs. This method allows for monitoring EVG, DTG and RPV in the plasma of HIV‐positive patients using HPLC‐UV.</description><identifier>ISSN: 0269-3879</identifier><identifier>EISSN: 1099-0801</identifier><identifier>DOI: 10.1002/bmc.4274</identifier><identifier>PMID: 29726595</identifier><language>eng</language><publisher>England</publisher><subject>antiretrovirals ; elvitegravir ; HIV ; HPLC‐UV ; therapeutic drug monitoring</subject><ispartof>Biomedical chromatography, 2018-09, Vol.32 (9), p.e4274-n/a</ispartof><rights>Copyright © 2018 John Wiley & Sons, Ltd.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3214-1de6bc1d6e5e816d07adc9338bcf326fa5b371921407a90a17cd064508871d663</citedby><cites>FETCH-LOGICAL-c3214-1de6bc1d6e5e816d07adc9338bcf326fa5b371921407a90a17cd064508871d663</cites><orcidid>0000-0002-1321-4126</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Fbmc.4274$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Fbmc.4274$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1417,27924,27925,45574,45575</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/29726595$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Tempestilli, Massimo</creatorcontrib><creatorcontrib>Ammassari, Adriana</creatorcontrib><creatorcontrib>D'Avolio, Antonio</creatorcontrib><creatorcontrib>Cicalini, Stefania</creatorcontrib><creatorcontrib>Gallo, Anna Loredana</creatorcontrib><creatorcontrib>Fazio, Simone</creatorcontrib><creatorcontrib>Antinori, Andrea</creatorcontrib><creatorcontrib>Pucillo, Leopoldo P.</creatorcontrib><title>Development and validation of an HPLC‐UV method for quantification of elvitegravir and two other new antiretrovirals, dolutegravir and rilpivirine, in the plasma of HIV‐positive patients</title><title>Biomedical chromatography</title><addtitle>Biomed Chromatogr</addtitle><description>Therapeutic drug monitoring may be crucial in selected clinical conditions for the management of HIV infection. In recent years, new antiretrovirals have been introduced and in particular elvitegravir (EVG) is now recommended for first‐line and simplification treatment as well as dolutegravir (DTG) and rilpivirine (RPV). The aim of this study was to develop and validate a high‐performance liquid chromatography–ultraviolet (HPLC‐UV) method for determining EVG and new antiretrovirals DTG and RPV in human plasma. Solid‐phase extraction was applied to a 600 μL plasma sample. Chromatographic separation of the three drugs and internal standard was achieved with a gradient of acetonitrile and phosphate buffer on a C18 reverse‐phase analytical column with a 20 min analytical run time. EVG and DTG were detected at 265 nm and RPV at 290 nm. Mean intra‐ and inter‐day precisions were < 10%; the mean accuracy was <15%. Extraction recovery ranged between 105 and 82% for the drugs analyzed. Calibration curves were optimized according to the expected ranges of drug concentrations in patients; the coefficient of determination was >0.997 for all drugs. This method allows for monitoring EVG, DTG and RPV in the plasma of HIV‐positive patients using HPLC‐UV.</description><subject>antiretrovirals</subject><subject>elvitegravir</subject><subject>HIV</subject><subject>HPLC‐UV</subject><subject>therapeutic drug monitoring</subject><issn>0269-3879</issn><issn>1099-0801</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2018</creationdate><recordtype>article</recordtype><recordid>eNp1kc1O3DAURi1UVKa0Ek-AvOyCgO0kTryk059BmgoWhW3k2DfFyImD7WTEjkfgifowfZJ6gIK66Mqyv3OPr_QhdEDJMSWEnbS9Oi5YVeygBSVCZKQm9A1aEMZFlteV2EPvQrghhAjOqrdoj4mK8VKUC_TrM8xg3djDELEcNJ6lNVpG4wbsuvSCVxfr5e_7h8sr3EO8dhp3zuPbSQ7RdEa9kGBnE-Gnl7Pxj6K4cdjFa_B4gA3e4h6idymWNhxh7ez0D--NHU26mAGOsBlwGsWjlaGXW_3q7CotMbpgoplTkP5NG4f3aLdLOvjwfO6jy69ffixX2fr829nydJ2pnNEioxp4q6jmUEJNuSaV1Erked2qLme8k2WbV1QkNCWCSFopTXhRkrqu0hTP99HHJ-_o3e0EITa9CQqslQO4KTSM5CUrSs7JK6q8C8FD14ze9NLfNZQ027aa1FazbSuhh8_Wqe1Bv4B_60lA9gRsjIW7_4qaT9-Xj8I_302jqA</recordid><startdate>201809</startdate><enddate>201809</enddate><creator>Tempestilli, Massimo</creator><creator>Ammassari, Adriana</creator><creator>D'Avolio, Antonio</creator><creator>Cicalini, Stefania</creator><creator>Gallo, Anna Loredana</creator><creator>Fazio, Simone</creator><creator>Antinori, Andrea</creator><creator>Pucillo, Leopoldo P.</creator><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0002-1321-4126</orcidid></search><sort><creationdate>201809</creationdate><title>Development and validation of an HPLC‐UV method for quantification of elvitegravir and two other new antiretrovirals, dolutegravir and rilpivirine, in the plasma of HIV‐positive patients</title><author>Tempestilli, Massimo ; Ammassari, Adriana ; D'Avolio, Antonio ; Cicalini, Stefania ; Gallo, Anna Loredana ; Fazio, Simone ; Antinori, Andrea ; Pucillo, Leopoldo P.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3214-1de6bc1d6e5e816d07adc9338bcf326fa5b371921407a90a17cd064508871d663</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2018</creationdate><topic>antiretrovirals</topic><topic>elvitegravir</topic><topic>HIV</topic><topic>HPLC‐UV</topic><topic>therapeutic drug monitoring</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Tempestilli, Massimo</creatorcontrib><creatorcontrib>Ammassari, Adriana</creatorcontrib><creatorcontrib>D'Avolio, Antonio</creatorcontrib><creatorcontrib>Cicalini, Stefania</creatorcontrib><creatorcontrib>Gallo, Anna Loredana</creatorcontrib><creatorcontrib>Fazio, Simone</creatorcontrib><creatorcontrib>Antinori, Andrea</creatorcontrib><creatorcontrib>Pucillo, Leopoldo P.</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Biomedical chromatography</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Tempestilli, Massimo</au><au>Ammassari, Adriana</au><au>D'Avolio, Antonio</au><au>Cicalini, Stefania</au><au>Gallo, Anna Loredana</au><au>Fazio, Simone</au><au>Antinori, Andrea</au><au>Pucillo, Leopoldo P.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Development and validation of an HPLC‐UV method for quantification of elvitegravir and two other new antiretrovirals, dolutegravir and rilpivirine, in the plasma of HIV‐positive patients</atitle><jtitle>Biomedical chromatography</jtitle><addtitle>Biomed Chromatogr</addtitle><date>2018-09</date><risdate>2018</risdate><volume>32</volume><issue>9</issue><spage>e4274</spage><epage>n/a</epage><pages>e4274-n/a</pages><issn>0269-3879</issn><eissn>1099-0801</eissn><abstract>Therapeutic drug monitoring may be crucial in selected clinical conditions for the management of HIV infection. In recent years, new antiretrovirals have been introduced and in particular elvitegravir (EVG) is now recommended for first‐line and simplification treatment as well as dolutegravir (DTG) and rilpivirine (RPV). The aim of this study was to develop and validate a high‐performance liquid chromatography–ultraviolet (HPLC‐UV) method for determining EVG and new antiretrovirals DTG and RPV in human plasma. Solid‐phase extraction was applied to a 600 μL plasma sample. Chromatographic separation of the three drugs and internal standard was achieved with a gradient of acetonitrile and phosphate buffer on a C18 reverse‐phase analytical column with a 20 min analytical run time. EVG and DTG were detected at 265 nm and RPV at 290 nm. Mean intra‐ and inter‐day precisions were < 10%; the mean accuracy was <15%. Extraction recovery ranged between 105 and 82% for the drugs analyzed. Calibration curves were optimized according to the expected ranges of drug concentrations in patients; the coefficient of determination was >0.997 for all drugs. This method allows for monitoring EVG, DTG and RPV in the plasma of HIV‐positive patients using HPLC‐UV.</abstract><cop>England</cop><pmid>29726595</pmid><doi>10.1002/bmc.4274</doi><tpages>4</tpages><orcidid>https://orcid.org/0000-0002-1321-4126</orcidid></addata></record> |
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| source | Wiley Journals |
| subjects | antiretrovirals elvitegravir HIV HPLC‐UV therapeutic drug monitoring |
| title | Development and validation of an HPLC‐UV method for quantification of elvitegravir and two other new antiretrovirals, dolutegravir and rilpivirine, in the plasma of HIV‐positive patients |
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