Short Communication: Cytological Alterations Produced by Sweet Potato Mild Speckling Virus
The potyvirus sweet potato mild speckling (SPMSV) has the biological properties and the coat protein sequence already described. In this work, cytological alterations and the intracellular localization in Ipomoea setosa and Ipomoea batatas was studied. The observations were carried out by means of t...
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Veröffentlicht in: | Journal of phytopathology 2006-08, Vol.154 (7-8), p.504-507 |
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description | The potyvirus sweet potato mild speckling (SPMSV) has the biological properties and the coat protein sequence already described. In this work, cytological alterations and the intracellular localization in Ipomoea setosa and Ipomoea batatas was studied. The observations were carried out by means of transmission electron microscopy, complemented with immunogold techniques for the viral localization with SPMSV antiserum of local production. The observations carried out showed almost no alteration on cell components but the presence of cylindrical inclusion in the cytoplasm (bundles, laminate aggregates, and pinwheels, neither circles nor scrolls) belonging to the type-2 in the classification of Edwardson and Christie (Cylindrical Inclusions. Bulletin 894, 1996, pp. 1-11). Gold particles were localized in cytoplasms of all tissues of the leaf. |
doi_str_mv | 10.1111/j.1439-0434.2006.01131.x |
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In this work, cytological alterations and the intracellular localization in Ipomoea setosa and Ipomoea batatas was studied. The observations were carried out by means of transmission electron microscopy, complemented with immunogold techniques for the viral localization with SPMSV antiserum of local production. The observations carried out showed almost no alteration on cell components but the presence of cylindrical inclusion in the cytoplasm (bundles, laminate aggregates, and pinwheels, neither circles nor scrolls) belonging to the type-2 in the classification of Edwardson and Christie (Cylindrical Inclusions. Bulletin 894, 1996, pp. 1-11). 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In this work, cytological alterations and the intracellular localization in Ipomoea setosa and Ipomoea batatas was studied. The observations were carried out by means of transmission electron microscopy, complemented with immunogold techniques for the viral localization with SPMSV antiserum of local production. The observations carried out showed almost no alteration on cell components but the presence of cylindrical inclusion in the cytoplasm (bundles, laminate aggregates, and pinwheels, neither circles nor scrolls) belonging to the type-2 in the classification of Edwardson and Christie (Cylindrical Inclusions. Bulletin 894, 1996, pp. 1-11). Gold particles were localized in cytoplasms of all tissues of the leaf.</description><subject>Ipomoea batatas</subject><subject>Ipomoea setosa</subject><subject>Potyvirus</subject><subject>Solanum tuberosum</subject><subject>sweet potato mild speckling virus</subject><issn>0931-1785</issn><issn>1439-0434</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2006</creationdate><recordtype>article</recordtype><recordid>eNqNjk9PwkAUxDdEEyr6Hd6JW9e33QLCzTQYLyYkJRy4kNo-cXHbh_snyLe3MYYzc5nMb-YwQoBCqXo9HqTK9TzFXOcyQ5xKVEor-TMQyaW4EQnOtUrV7GkyFHfeHxAz1IiJ2Jaf7AIU3LaxM3UVDHcLKM6BLe_7bOHZBnJ_3MPKcRNrauD9DOWJKMCKQxUY3oxtoDxS_WVNt4eNcdHfi9uPynp6-PeRGL8s18VrenT8HcmHXWt8TdZWHXH0u_5RPptkU3318Bek0U4Q</recordid><startdate>20060801</startdate><enddate>20060801</enddate><creator>Nome, C F</creator><creator>Laguna, I G</creator><creator>Nome, S F</creator><scope>7T7</scope><scope>7U9</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>H94</scope><scope>P64</scope></search><sort><creationdate>20060801</creationdate><title>Short Communication: Cytological Alterations Produced by Sweet Potato Mild Speckling Virus</title><author>Nome, C F ; Laguna, I G ; Nome, S F</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-proquest_miscellaneous_203475263</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2006</creationdate><topic>Ipomoea batatas</topic><topic>Ipomoea setosa</topic><topic>Potyvirus</topic><topic>Solanum tuberosum</topic><topic>sweet potato mild speckling virus</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Nome, C F</creatorcontrib><creatorcontrib>Laguna, I G</creatorcontrib><creatorcontrib>Nome, S F</creatorcontrib><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Biotechnology and BioEngineering Abstracts</collection><jtitle>Journal of phytopathology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Nome, C F</au><au>Laguna, I G</au><au>Nome, S F</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Short Communication: Cytological Alterations Produced by Sweet Potato Mild Speckling Virus</atitle><jtitle>Journal of phytopathology</jtitle><date>2006-08-01</date><risdate>2006</risdate><volume>154</volume><issue>7-8</issue><spage>504</spage><epage>507</epage><pages>504-507</pages><issn>0931-1785</issn><eissn>1439-0434</eissn><abstract>The potyvirus sweet potato mild speckling (SPMSV) has the biological properties and the coat protein sequence already described. In this work, cytological alterations and the intracellular localization in Ipomoea setosa and Ipomoea batatas was studied. The observations were carried out by means of transmission electron microscopy, complemented with immunogold techniques for the viral localization with SPMSV antiserum of local production. The observations carried out showed almost no alteration on cell components but the presence of cylindrical inclusion in the cytoplasm (bundles, laminate aggregates, and pinwheels, neither circles nor scrolls) belonging to the type-2 in the classification of Edwardson and Christie (Cylindrical Inclusions. Bulletin 894, 1996, pp. 1-11). Gold particles were localized in cytoplasms of all tissues of the leaf.</abstract><doi>10.1111/j.1439-0434.2006.01131.x</doi></addata></record> |
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source | Wiley Online Library Journals Frontfile Complete |
subjects | Ipomoea batatas Ipomoea setosa Potyvirus Solanum tuberosum sweet potato mild speckling virus |
title | Short Communication: Cytological Alterations Produced by Sweet Potato Mild Speckling Virus |
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