Reconstruction of three-dimensional human skin model composed of dendritic cells, keratinocytes and fibroblasts utilizing a handy scaffold of collagen vitrigel membrane

We previously we attempted to make a three-dimensional human skin model consisting of three different cells, dendritic cells, keratinocytes and fibroblasts (KDF-Skin) to evaluate immunoreactions in human skin; however, this model had various problems; for example (1) the incubation period for the co...

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Veröffentlicht in:	Toxicology in vitro 2009-03, Vol.23 (2), p.333-337
Hauptverfasser:	Uchino, T., Takezawa, T., Ikarashi, Y.
Format:	Artikel
Sprache:	eng
Schlagworte:	Animal Testing Alternatives B7-2 Antigen - metabolism CD86 Cell Culture Techniques - methods Cells, Cultured Coculture Techniques - methods Collagen Type I - chemistry Collagen vitrigel membrane Cytokines - metabolism Dendritic cells Dendritic Cells - cytology Dendritic Cells - drug effects Dendritic Cells - metabolism Fibroblasts - cytology Fibroblasts - drug effects Fibroblasts - metabolism Gels Humans Irritants - toxicity Keratinocytes - cytology Keratinocytes - drug effects Keratinocytes - metabolism Membranes, Artificial Models, Biological Skin sensitization Skin, Artificial Three-dimensional human skin model Time Factors Tissue Scaffolds
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