Occurrence and molecular characterization of Clinostomum complanatum (Trematoda: Clinostomidae) in freshwater fishes caught from Turkey
The metacercariae of Clinostomum species which known as yellow grubs have zoonotic potential by infecting humans. In the present study, a total of 403 freshwater fish specimens belonging to different genera from Central Anatolia Region of Turkey were examined for yellow grub metacercariae infections...
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| Veröffentlicht in: | Parasitology research (1987) 2018-07, Vol.117 (7), p.2117-2124 |
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| container_title | Parasitology research (1987) |
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| creator | Simsek, Emrah Yildirim, Alparslan Yilmaz, Erdal Inci, Abdullah Duzlu, Onder Onder, Zuhal Ciloglu, Arif Yetismis, Gamze Pekmezci, Gokmen Zafer |
| description | The metacercariae of
Clinostomum
species which known as yellow grubs have zoonotic potential by infecting humans. In the present study, a total of 403 freshwater fish specimens belonging to different genera from Central Anatolia Region of Turkey were examined for yellow grub metacercariae infections. Only three specimens belonging to
Squalius cephalus
were found to be infected with metacercariae with a prevalence on this host species of 2.4% and an overall prevalence of 0.7%. All the metacercariae were morphologically identified as
Clinostomum complanatum
. Partial fragments of mitochondrial cytochrome oxidase I (mt-COI) gene and internal transcribed spacer 2 (ITS-2) were amplified for sequence and phylogenetic analyses. The sequence analyses of ITS-2 and mt-COI revealed three and nine polymorphic sites leading to detection of four and five haplotypes within the related gene regions, respectively. Moreover, the intraspecific genetic distances for
C
.
complanatum
isolates ranged from 0.0 to 0.7% for ITS-2 and 0.0 to 1.4% for mt-COI data sets. Consequently, the present study has provided first combined morphologic and molecular data on
C
.
complanatum
infecting Turkish freshwater fishes. |
| doi_str_mv | 10.1007/s00436-018-5898-3 |
| format | Article |
| fullrecord | <record><control><sourceid>gale_proqu</sourceid><recordid>TN_cdi_proquest_miscellaneous_2034291106</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><galeid>A543515200</galeid><sourcerecordid>A543515200</sourcerecordid><originalsourceid>FETCH-LOGICAL-c411t-40b9029bbd46ba4a113093ede95f86af07042ccc08a186a8292d5210ed70014c3</originalsourceid><addsrcrecordid>eNp1kc9u1DAQxi0EokvhAbggS1zaQ8rYsZOYW7WigFSpl-UcOc5k1yWxFztRVV6A12aqFCohIR_8Z37faD5_jL0VcCEA6g8ZQJVVAaIpdGOaonzGNkKVshBG6-dsA4bOIER5wl7lfAsg6kqpl-xEmlqKSjcb9uvGuSUlDA65DT2f4ohuGW3i7mCTdTMm_9POPgYeB74dfYh5jtMycRen42iDnel8tks42Tn29uMT43uL59wHPiTMhztLrfjg8wEzd3bZH2YqxInvlvQd71-zF4MdM7553E_Zt6tPu-2X4vrm89ft5XXhlBBzoaAzIE3X9arqrLLkjUxij0YPTWUHqEFJ5xw0VtC9kUb2WgrAvib3ypWn7Gzte0zxx4J5biefHY7kBOOSWwmlkkYIqAh9_w96G5cUaDqidFUboWtJ1MVK7e2IrQ9DnOnbaPU4eRcDDp7eL7UqtdASgARiFbgUc044tMfkJ5vuWwHtQ6ztGmtLsbYPsbYlad49jrJ0E_Z_FX9yJECuQKZS2GN6mvX_XX8Dy3KuXA</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2056791572</pqid></control><display><type>article</type><title>Occurrence and molecular characterization of Clinostomum complanatum (Trematoda: Clinostomidae) in freshwater fishes caught from Turkey</title><source>MEDLINE</source><source>SpringerLink Journals - AutoHoldings</source><creator>Simsek, Emrah ; Yildirim, Alparslan ; Yilmaz, Erdal ; Inci, Abdullah ; Duzlu, Onder ; Onder, Zuhal ; Ciloglu, Arif ; Yetismis, Gamze ; Pekmezci, Gokmen Zafer</creator><creatorcontrib>Simsek, Emrah ; Yildirim, Alparslan ; Yilmaz, Erdal ; Inci, Abdullah ; Duzlu, Onder ; Onder, Zuhal ; Ciloglu, Arif ; Yetismis, Gamze ; Pekmezci, Gokmen Zafer</creatorcontrib><description>The metacercariae of
Clinostomum
species which known as yellow grubs have zoonotic potential by infecting humans. In the present study, a total of 403 freshwater fish specimens belonging to different genera from Central Anatolia Region of Turkey were examined for yellow grub metacercariae infections. Only three specimens belonging to
Squalius cephalus
were found to be infected with metacercariae with a prevalence on this host species of 2.4% and an overall prevalence of 0.7%. All the metacercariae were morphologically identified as
Clinostomum complanatum
. Partial fragments of mitochondrial cytochrome oxidase I (mt-COI) gene and internal transcribed spacer 2 (ITS-2) were amplified for sequence and phylogenetic analyses. The sequence analyses of ITS-2 and mt-COI revealed three and nine polymorphic sites leading to detection of four and five haplotypes within the related gene regions, respectively. Moreover, the intraspecific genetic distances for
C
.
complanatum
isolates ranged from 0.0 to 0.7% for ITS-2 and 0.0 to 1.4% for mt-COI data sets. Consequently, the present study has provided first combined morphologic and molecular data on
C
.
complanatum
infecting Turkish freshwater fishes.</description><identifier>ISSN: 0932-0113</identifier><identifier>EISSN: 1432-1955</identifier><identifier>DOI: 10.1007/s00436-018-5898-3</identifier><identifier>PMID: 29721658</identifier><language>eng</language><publisher>Berlin/Heidelberg: Springer Berlin Heidelberg</publisher><subject>Animals ; Biomedical and Life Sciences ; Biomedicine ; Clinostomum ; Cytochrome oxidase I ; DNA, Intergenic - genetics ; Electron Transport Complex IV - genetics ; Fish ; Fish Diseases - epidemiology ; Fish Diseases - parasitology ; Fishes ; Flukes ; Freshwater fishes ; Genes, Mitochondrial ; Genetic distance ; Haplotypes ; Health aspects ; Host-parasite relationships ; Humans ; Immunology ; Medical Microbiology ; Metacercariae - classification ; Metacercariae - genetics ; Metacercariae - isolation & purification ; Microbiology ; Mitochondria ; Observations ; Original Paper ; Phylogeny ; Spacer ; Trematoda - classification ; Trematoda - genetics ; Trematoda - isolation & purification ; Trematode Infections - epidemiology ; Trematode Infections - parasitology ; Trematode Infections - veterinary ; Turkey - epidemiology</subject><ispartof>Parasitology research (1987), 2018-07, Vol.117 (7), p.2117-2124</ispartof><rights>Springer-Verlag GmbH Germany, part of Springer Nature 2018</rights><rights>COPYRIGHT 2018 Springer</rights><rights>Copyright Springer Science & Business Media 2018</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c411t-40b9029bbd46ba4a113093ede95f86af07042ccc08a186a8292d5210ed70014c3</citedby><cites>FETCH-LOGICAL-c411t-40b9029bbd46ba4a113093ede95f86af07042ccc08a186a8292d5210ed70014c3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s00436-018-5898-3$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s00436-018-5898-3$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,780,784,27923,27924,41487,42556,51318</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/29721658$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Simsek, Emrah</creatorcontrib><creatorcontrib>Yildirim, Alparslan</creatorcontrib><creatorcontrib>Yilmaz, Erdal</creatorcontrib><creatorcontrib>Inci, Abdullah</creatorcontrib><creatorcontrib>Duzlu, Onder</creatorcontrib><creatorcontrib>Onder, Zuhal</creatorcontrib><creatorcontrib>Ciloglu, Arif</creatorcontrib><creatorcontrib>Yetismis, Gamze</creatorcontrib><creatorcontrib>Pekmezci, Gokmen Zafer</creatorcontrib><title>Occurrence and molecular characterization of Clinostomum complanatum (Trematoda: Clinostomidae) in freshwater fishes caught from Turkey</title><title>Parasitology research (1987)</title><addtitle>Parasitol Res</addtitle><addtitle>Parasitol Res</addtitle><description>The metacercariae of
Clinostomum
species which known as yellow grubs have zoonotic potential by infecting humans. In the present study, a total of 403 freshwater fish specimens belonging to different genera from Central Anatolia Region of Turkey were examined for yellow grub metacercariae infections. Only three specimens belonging to
Squalius cephalus
were found to be infected with metacercariae with a prevalence on this host species of 2.4% and an overall prevalence of 0.7%. All the metacercariae were morphologically identified as
Clinostomum complanatum
. Partial fragments of mitochondrial cytochrome oxidase I (mt-COI) gene and internal transcribed spacer 2 (ITS-2) were amplified for sequence and phylogenetic analyses. The sequence analyses of ITS-2 and mt-COI revealed three and nine polymorphic sites leading to detection of four and five haplotypes within the related gene regions, respectively. Moreover, the intraspecific genetic distances for
C
.
complanatum
isolates ranged from 0.0 to 0.7% for ITS-2 and 0.0 to 1.4% for mt-COI data sets. Consequently, the present study has provided first combined morphologic and molecular data on
C
.
complanatum
infecting Turkish freshwater fishes.</description><subject>Animals</subject><subject>Biomedical and Life Sciences</subject><subject>Biomedicine</subject><subject>Clinostomum</subject><subject>Cytochrome oxidase I</subject><subject>DNA, Intergenic - genetics</subject><subject>Electron Transport Complex IV - genetics</subject><subject>Fish</subject><subject>Fish Diseases - epidemiology</subject><subject>Fish Diseases - parasitology</subject><subject>Fishes</subject><subject>Flukes</subject><subject>Freshwater fishes</subject><subject>Genes, Mitochondrial</subject><subject>Genetic distance</subject><subject>Haplotypes</subject><subject>Health aspects</subject><subject>Host-parasite relationships</subject><subject>Humans</subject><subject>Immunology</subject><subject>Medical Microbiology</subject><subject>Metacercariae - classification</subject><subject>Metacercariae - genetics</subject><subject>Metacercariae - isolation & purification</subject><subject>Microbiology</subject><subject>Mitochondria</subject><subject>Observations</subject><subject>Original Paper</subject><subject>Phylogeny</subject><subject>Spacer</subject><subject>Trematoda - classification</subject><subject>Trematoda - genetics</subject><subject>Trematoda - isolation & purification</subject><subject>Trematode Infections - epidemiology</subject><subject>Trematode Infections - parasitology</subject><subject>Trematode Infections - veterinary</subject><subject>Turkey - epidemiology</subject><issn>0932-0113</issn><issn>1432-1955</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2018</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1kc9u1DAQxi0EokvhAbggS1zaQ8rYsZOYW7WigFSpl-UcOc5k1yWxFztRVV6A12aqFCohIR_8Z37faD5_jL0VcCEA6g8ZQJVVAaIpdGOaonzGNkKVshBG6-dsA4bOIER5wl7lfAsg6kqpl-xEmlqKSjcb9uvGuSUlDA65DT2f4ohuGW3i7mCTdTMm_9POPgYeB74dfYh5jtMycRen42iDnel8tks42Tn29uMT43uL59wHPiTMhztLrfjg8wEzd3bZH2YqxInvlvQd71-zF4MdM7553E_Zt6tPu-2X4vrm89ft5XXhlBBzoaAzIE3X9arqrLLkjUxij0YPTWUHqEFJ5xw0VtC9kUb2WgrAvib3ypWn7Gzte0zxx4J5biefHY7kBOOSWwmlkkYIqAh9_w96G5cUaDqidFUboWtJ1MVK7e2IrQ9DnOnbaPU4eRcDDp7eL7UqtdASgARiFbgUc044tMfkJ5vuWwHtQ6ztGmtLsbYPsbYlad49jrJ0E_Z_FX9yJECuQKZS2GN6mvX_XX8Dy3KuXA</recordid><startdate>20180701</startdate><enddate>20180701</enddate><creator>Simsek, Emrah</creator><creator>Yildirim, Alparslan</creator><creator>Yilmaz, Erdal</creator><creator>Inci, Abdullah</creator><creator>Duzlu, Onder</creator><creator>Onder, Zuhal</creator><creator>Ciloglu, Arif</creator><creator>Yetismis, Gamze</creator><creator>Pekmezci, Gokmen Zafer</creator><general>Springer Berlin Heidelberg</general><general>Springer</general><general>Springer Nature B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20180701</creationdate><title>Occurrence and molecular characterization of Clinostomum complanatum (Trematoda: Clinostomidae) in freshwater fishes caught from Turkey</title><author>Simsek, Emrah ; Yildirim, Alparslan ; Yilmaz, Erdal ; Inci, Abdullah ; Duzlu, Onder ; Onder, Zuhal ; Ciloglu, Arif ; Yetismis, Gamze ; Pekmezci, Gokmen Zafer</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c411t-40b9029bbd46ba4a113093ede95f86af07042ccc08a186a8292d5210ed70014c3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2018</creationdate><topic>Animals</topic><topic>Biomedical and Life Sciences</topic><topic>Biomedicine</topic><topic>Clinostomum</topic><topic>Cytochrome oxidase I</topic><topic>DNA, Intergenic - genetics</topic><topic>Electron Transport Complex IV - genetics</topic><topic>Fish</topic><topic>Fish Diseases - epidemiology</topic><topic>Fish Diseases - parasitology</topic><topic>Fishes</topic><topic>Flukes</topic><topic>Freshwater fishes</topic><topic>Genes, Mitochondrial</topic><topic>Genetic distance</topic><topic>Haplotypes</topic><topic>Health aspects</topic><topic>Host-parasite relationships</topic><topic>Humans</topic><topic>Immunology</topic><topic>Medical Microbiology</topic><topic>Metacercariae - classification</topic><topic>Metacercariae - genetics</topic><topic>Metacercariae - isolation & purification</topic><topic>Microbiology</topic><topic>Mitochondria</topic><topic>Observations</topic><topic>Original Paper</topic><topic>Phylogeny</topic><topic>Spacer</topic><topic>Trematoda - classification</topic><topic>Trematoda - genetics</topic><topic>Trematoda - isolation & purification</topic><topic>Trematode Infections - epidemiology</topic><topic>Trematode Infections - parasitology</topic><topic>Trematode Infections - veterinary</topic><topic>Turkey - epidemiology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Simsek, Emrah</creatorcontrib><creatorcontrib>Yildirim, Alparslan</creatorcontrib><creatorcontrib>Yilmaz, Erdal</creatorcontrib><creatorcontrib>Inci, Abdullah</creatorcontrib><creatorcontrib>Duzlu, Onder</creatorcontrib><creatorcontrib>Onder, Zuhal</creatorcontrib><creatorcontrib>Ciloglu, Arif</creatorcontrib><creatorcontrib>Yetismis, Gamze</creatorcontrib><creatorcontrib>Pekmezci, Gokmen Zafer</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Parasitology research (1987)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Simsek, Emrah</au><au>Yildirim, Alparslan</au><au>Yilmaz, Erdal</au><au>Inci, Abdullah</au><au>Duzlu, Onder</au><au>Onder, Zuhal</au><au>Ciloglu, Arif</au><au>Yetismis, Gamze</au><au>Pekmezci, Gokmen Zafer</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Occurrence and molecular characterization of Clinostomum complanatum (Trematoda: Clinostomidae) in freshwater fishes caught from Turkey</atitle><jtitle>Parasitology research (1987)</jtitle><stitle>Parasitol Res</stitle><addtitle>Parasitol Res</addtitle><date>2018-07-01</date><risdate>2018</risdate><volume>117</volume><issue>7</issue><spage>2117</spage><epage>2124</epage><pages>2117-2124</pages><issn>0932-0113</issn><eissn>1432-1955</eissn><abstract>The metacercariae of
Clinostomum
species which known as yellow grubs have zoonotic potential by infecting humans. In the present study, a total of 403 freshwater fish specimens belonging to different genera from Central Anatolia Region of Turkey were examined for yellow grub metacercariae infections. Only three specimens belonging to
Squalius cephalus
were found to be infected with metacercariae with a prevalence on this host species of 2.4% and an overall prevalence of 0.7%. All the metacercariae were morphologically identified as
Clinostomum complanatum
. Partial fragments of mitochondrial cytochrome oxidase I (mt-COI) gene and internal transcribed spacer 2 (ITS-2) were amplified for sequence and phylogenetic analyses. The sequence analyses of ITS-2 and mt-COI revealed three and nine polymorphic sites leading to detection of four and five haplotypes within the related gene regions, respectively. Moreover, the intraspecific genetic distances for
C
.
complanatum
isolates ranged from 0.0 to 0.7% for ITS-2 and 0.0 to 1.4% for mt-COI data sets. Consequently, the present study has provided first combined morphologic and molecular data on
C
.
complanatum
infecting Turkish freshwater fishes.</abstract><cop>Berlin/Heidelberg</cop><pub>Springer Berlin Heidelberg</pub><pmid>29721658</pmid><doi>10.1007/s00436-018-5898-3</doi><tpages>8</tpages></addata></record> |
| fulltext | fulltext |
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| ispartof | Parasitology research (1987), 2018-07, Vol.117 (7), p.2117-2124 |
| issn | 0932-0113 1432-1955 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_2034291106 |
| source | MEDLINE; SpringerLink Journals - AutoHoldings |
| subjects | Animals Biomedical and Life Sciences Biomedicine Clinostomum Cytochrome oxidase I DNA, Intergenic - genetics Electron Transport Complex IV - genetics Fish Fish Diseases - epidemiology Fish Diseases - parasitology Fishes Flukes Freshwater fishes Genes, Mitochondrial Genetic distance Haplotypes Health aspects Host-parasite relationships Humans Immunology Medical Microbiology Metacercariae - classification Metacercariae - genetics Metacercariae - isolation & purification Microbiology Mitochondria Observations Original Paper Phylogeny Spacer Trematoda - classification Trematoda - genetics Trematoda - isolation & purification Trematode Infections - epidemiology Trematode Infections - parasitology Trematode Infections - veterinary Turkey - epidemiology |
| title | Occurrence and molecular characterization of Clinostomum complanatum (Trematoda: Clinostomidae) in freshwater fishes caught from Turkey |
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