A Neurotoxic Secretory Phospholipase A2 Induces Apoptosis in Motoneuron‐like Cells
Ammodytoxin A (AtxA) is a presynaptically neurotoxic secretory phospholipase A2 from snake venom. The aim of this study was to investigate the mechanism of its cytotoxicity expressed against mouse motoneuronal NSC34 cells. AtxA displayed a potent dose‐ and time‐dependent cytotoxicity that was associ...
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| Veröffentlicht in: | Annals of the New York Academy of Sciences 2009-01, Vol.1152 (1), p.215-224 |
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| creator | Pražnikar, Zala Jenko Petan, Toni Pungerčar, Jože |
| description | Ammodytoxin A (AtxA) is a presynaptically neurotoxic secretory phospholipase A2 from snake venom. The aim of this study was to investigate the mechanism of its cytotoxicity expressed against mouse motoneuronal NSC34 cells. AtxA displayed a potent dose‐ and time‐dependent cytotoxicity that was associated with apoptosis and not necrosis, as revealed by a reduction of mitochondrial membrane potential, activation of caspase‐3, and by the absence of propidium iodide staining. The cytotoxic‐ and apoptosis‐inducing effects of AtxA were specific for the motoneuronal cells; human embryonic kidney (HEK293) and mouse myoblast (C2C12) cells were shown to be resistant to the toxin. |
| doi_str_mv | 10.1111/j.1749-6632.2008.03999.x |
| format | Article |
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The cytotoxic‐ and apoptosis‐inducing effects of AtxA were specific for the motoneuronal cells; human embryonic kidney (HEK293) and mouse myoblast (C2C12) cells were shown to be resistant to the toxin.</description><identifier>ISSN: 0077-8923</identifier><identifier>EISSN: 1749-6632</identifier><identifier>EISSN: 1930-6547</identifier><identifier>DOI: 10.1111/j.1749-6632.2008.03999.x</identifier><identifier>PMID: 19161393</identifier><language>eng</language><publisher>Malden, USA: Blackwell Publishing Inc</publisher><subject>ammodytoxin A ; Animals ; apoptosis ; Apoptosis - drug effects ; Caspase 3 - metabolism ; Cell Line ; cytotoxicity ; Humans ; Membrane Potential, Mitochondrial - drug effects ; Mice ; motoneuronal cells ; Motor Neurons - cytology ; Motor Neurons - drug effects ; Motor Neurons - enzymology ; phospholipase A2 ; Phospholipases A2 - metabolism ; Phospholipases A2 - secretion ; Viper Venoms - toxicity</subject><ispartof>Annals of the New York Academy of Sciences, 2009-01, Vol.1152 (1), p.215-224</ispartof><rights>2009 New York Academy of Sciences</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fj.1749-6632.2008.03999.x$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fj.1749-6632.2008.03999.x$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1417,27924,27925,45574,45575</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/19161393$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Pražnikar, Zala Jenko</creatorcontrib><creatorcontrib>Petan, Toni</creatorcontrib><creatorcontrib>Pungerčar, Jože</creatorcontrib><title>A Neurotoxic Secretory Phospholipase A2 Induces Apoptosis in Motoneuron‐like Cells</title><title>Annals of the New York Academy of Sciences</title><addtitle>Ann N Y Acad Sci</addtitle><description>Ammodytoxin A (AtxA) is a presynaptically neurotoxic secretory phospholipase A2 from snake venom. 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The cytotoxic‐ and apoptosis‐inducing effects of AtxA were specific for the motoneuronal cells; human embryonic kidney (HEK293) and mouse myoblast (C2C12) cells were shown to be resistant to the toxin.</description><subject>ammodytoxin A</subject><subject>Animals</subject><subject>apoptosis</subject><subject>Apoptosis - drug effects</subject><subject>Caspase 3 - metabolism</subject><subject>Cell Line</subject><subject>cytotoxicity</subject><subject>Humans</subject><subject>Membrane Potential, Mitochondrial - drug effects</subject><subject>Mice</subject><subject>motoneuronal cells</subject><subject>Motor Neurons - cytology</subject><subject>Motor Neurons - drug effects</subject><subject>Motor Neurons - enzymology</subject><subject>phospholipase A2</subject><subject>Phospholipases A2 - metabolism</subject><subject>Phospholipases A2 - secretion</subject><subject>Viper Venoms - toxicity</subject><issn>0077-8923</issn><issn>1749-6632</issn><issn>1930-6547</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2009</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpFkM1OwkAUhSdGI4i-gpmVu9b5KTOdlWmIPySIJuDC1WQolzBYOrXTRtj5CD6jT2ILqHdzb3LOuTn5EMKUhLSZ61VIZaQCITgLGSFxSLhSKtwcoe6fcIy6hEgZxIrxDjrzfkUIZXEkT1GHKiooV7yLpgkeQ126ym1siieQllC5coufl84XS5fZwnjACcPDfF6n4HFSuKJy3npsc_zY5PI2nn9_fmX2DfAAssyfo5OFyTxcHHYPvdzdTgcPwejpfjhIRkHBKG1aktgI4AaYmDW35IrJKAbTp7AQnPK-SGOygAhmSsSqL2NqZGQYkDlXIKTgPXS1_1uU7r0GX-m19WnTwOTgaq8Z4RGLOG-MlwdjPVvDXBelXZtyq385NIabveHDZrD914lueeuVbrHqFqtueesdb73R49dksrv5D2oNdEc</recordid><startdate>200901</startdate><enddate>200901</enddate><creator>Pražnikar, Zala Jenko</creator><creator>Petan, Toni</creator><creator>Pungerčar, Jože</creator><general>Blackwell Publishing Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7TK</scope></search><sort><creationdate>200901</creationdate><title>A Neurotoxic Secretory Phospholipase A2 Induces Apoptosis in Motoneuron‐like Cells</title><author>Pražnikar, Zala Jenko ; Petan, Toni ; Pungerčar, Jože</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-p2119-608a6e3ae26b6087392748ea51ef631356c80fe4eb96895781a74a2e0d39e6763</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2009</creationdate><topic>ammodytoxin A</topic><topic>Animals</topic><topic>apoptosis</topic><topic>Apoptosis - drug effects</topic><topic>Caspase 3 - metabolism</topic><topic>Cell Line</topic><topic>cytotoxicity</topic><topic>Humans</topic><topic>Membrane Potential, Mitochondrial - drug effects</topic><topic>Mice</topic><topic>motoneuronal cells</topic><topic>Motor Neurons - cytology</topic><topic>Motor Neurons - drug effects</topic><topic>Motor Neurons - enzymology</topic><topic>phospholipase A2</topic><topic>Phospholipases A2 - metabolism</topic><topic>Phospholipases A2 - secretion</topic><topic>Viper Venoms - toxicity</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Pražnikar, Zala Jenko</creatorcontrib><creatorcontrib>Petan, Toni</creatorcontrib><creatorcontrib>Pungerčar, Jože</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>Neurosciences Abstracts</collection><jtitle>Annals of the New York Academy of Sciences</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Pražnikar, Zala Jenko</au><au>Petan, Toni</au><au>Pungerčar, Jože</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A Neurotoxic Secretory Phospholipase A2 Induces Apoptosis in Motoneuron‐like Cells</atitle><jtitle>Annals of the New York Academy of Sciences</jtitle><addtitle>Ann N Y Acad Sci</addtitle><date>2009-01</date><risdate>2009</risdate><volume>1152</volume><issue>1</issue><spage>215</spage><epage>224</epage><pages>215-224</pages><issn>0077-8923</issn><eissn>1749-6632</eissn><eissn>1930-6547</eissn><abstract>Ammodytoxin A (AtxA) is a presynaptically neurotoxic secretory phospholipase A2 from snake venom. 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| source | MEDLINE; Wiley Online Library Journals Frontfile Complete |
| subjects | ammodytoxin A Animals apoptosis Apoptosis - drug effects Caspase 3 - metabolism Cell Line cytotoxicity Humans Membrane Potential, Mitochondrial - drug effects Mice motoneuronal cells Motor Neurons - cytology Motor Neurons - drug effects Motor Neurons - enzymology phospholipase A2 Phospholipases A2 - metabolism Phospholipases A2 - secretion Viper Venoms - toxicity |
| title | A Neurotoxic Secretory Phospholipase A2 Induces Apoptosis in Motoneuron‐like Cells |
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