Distribution of IgM, IgD and IgZ in mandarin fish, Siniperca chuatsi lymphoid tissues and their transcriptional changes after Flavobacterium columnare stimulation
The gene sequences of three different immunoglobulin (Ig) heavy chains, namely IgM, IgD and IgZ, were cloned from mandarin fish ( Siniperca chuatsi) recently. In this study the distribution of these three kinds of Ig-producing cells in lymphoid-related tissues as head kidney, spleen, gill and intest...
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| description | The gene sequences of three different immunoglobulin (Ig) heavy chains, namely IgM, IgD and IgZ, were cloned from mandarin fish (
Siniperca chuatsi) recently. In this study the distribution of these three kinds of Ig-producing cells in lymphoid-related tissues as head kidney, spleen, gill and intestine were investigated by using
in situ hybridization, and their transcriptional changes were also analyzed by quantitative real-time PCR during 8 weeks after immunization. IgM-producing cells could be detected obviously and abundantly in all the tissues examined. A few numbers of IgD and IgZ positive cells were both detected in head kidney and spleen. IgZ positive cells could be detected in gill moderately while IgD showed negative results, otherwise no IgD or IgZ positive cells could be detected in intestine. After stimulated with bacterial pathogen
Flavobacterium columnare G
4, the transcripts of these three Ig genes exhibited quite different kinetics. Significantly increased transcription of IgM gene was observed in almost all the tissues examined especially in boosted group. In contrast with IgM, seldom strong increase was examined for IgD and IgZ genes. For IgD, it seemed that the first injection could stimulate the immune response easier, since in almost all the tissues significant increase was detected at 1 or 2 weeks after injection. For IgZ, boosted injection could not enlarge the up-regulation of gene expression of first injection. This is the first case to report the transcriptional kinetics of three Ig genes in teleost after bacterin immunization. |
| doi_str_mv | 10.1016/j.aquaculture.2008.11.023 |
| format | Article |
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Siniperca chuatsi) recently. In this study the distribution of these three kinds of Ig-producing cells in lymphoid-related tissues as head kidney, spleen, gill and intestine were investigated by using
in situ hybridization, and their transcriptional changes were also analyzed by quantitative real-time PCR during 8 weeks after immunization. IgM-producing cells could be detected obviously and abundantly in all the tissues examined. A few numbers of IgD and IgZ positive cells were both detected in head kidney and spleen. IgZ positive cells could be detected in gill moderately while IgD showed negative results, otherwise no IgD or IgZ positive cells could be detected in intestine. After stimulated with bacterial pathogen
Flavobacterium columnare G
4, the transcripts of these three Ig genes exhibited quite different kinetics. Significantly increased transcription of IgM gene was observed in almost all the tissues examined especially in boosted group. In contrast with IgM, seldom strong increase was examined for IgD and IgZ genes. For IgD, it seemed that the first injection could stimulate the immune response easier, since in almost all the tissues significant increase was detected at 1 or 2 weeks after injection. For IgZ, boosted injection could not enlarge the up-regulation of gene expression of first injection. This is the first case to report the transcriptional kinetics of three Ig genes in teleost after bacterin immunization.</description><identifier>ISSN: 0044-8486</identifier><identifier>EISSN: 1873-5622</identifier><identifier>DOI: 10.1016/j.aquaculture.2008.11.023</identifier><identifier>CODEN: AQCLAL</identifier><language>eng</language><publisher>Amsterdam: Elsevier B.V</publisher><subject>Agnatha. Pisces ; Animal aquaculture ; animal pathogenic bacteria ; Animal productions ; Bacteria ; bacterial infections ; Biological and medical sciences ; Fish ; fish diseases ; Flavobacterium columnare ; Freshwater ; Fundamental and applied biological sciences. Psychology ; Gene expression ; gene expression regulation ; General aspects ; Genes ; immune response ; immunization ; Immunoglobulin ; immunoglobulin D ; immunoglobulin M ; immunoglobulin Z ; Immunoglobulins ; In situ hybridization ; lymphoid tissue ; Lymphoid tissues ; Mandarin fish ; Perciformes ; Quantitive real-time PCR ; Siniperca chuatsi ; Sinipercidae ; Teleostei ; Tissues ; transcription (genetics) ; Vertebrates: general zoology, morphology, phylogeny, systematics, cytogenetics, geographical distribution</subject><ispartof>Aquaculture, 2009-03, Vol.288 (1), p.14-21</ispartof><rights>2008 Elsevier B.V.</rights><rights>2009 INIST-CNRS</rights><rights>Copyright Elsevier Sequoia S.A. Mar 2, 2009</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c433t-54ec2999ef128bbfc844e16a2c67e2d5b15efda8dec0e8facf435e4534e403fd3</citedby><cites>FETCH-LOGICAL-c433t-54ec2999ef128bbfc844e16a2c67e2d5b15efda8dec0e8facf435e4534e403fd3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0044848608008429$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=21275075$$DView record in Pascal Francis$$Hfree_for_read</backlink></links><search><creatorcontrib>Tian, Jingyun</creatorcontrib><creatorcontrib>Sun, Baojian</creatorcontrib><creatorcontrib>Luo, Yanping</creatorcontrib><creatorcontrib>Zhang, Yongan</creatorcontrib><creatorcontrib>Nie, Pin</creatorcontrib><title>Distribution of IgM, IgD and IgZ in mandarin fish, Siniperca chuatsi lymphoid tissues and their transcriptional changes after Flavobacterium columnare stimulation</title><title>Aquaculture</title><description>The gene sequences of three different immunoglobulin (Ig) heavy chains, namely IgM, IgD and IgZ, were cloned from mandarin fish (
Siniperca chuatsi) recently. In this study the distribution of these three kinds of Ig-producing cells in lymphoid-related tissues as head kidney, spleen, gill and intestine were investigated by using
in situ hybridization, and their transcriptional changes were also analyzed by quantitative real-time PCR during 8 weeks after immunization. IgM-producing cells could be detected obviously and abundantly in all the tissues examined. A few numbers of IgD and IgZ positive cells were both detected in head kidney and spleen. IgZ positive cells could be detected in gill moderately while IgD showed negative results, otherwise no IgD or IgZ positive cells could be detected in intestine. After stimulated with bacterial pathogen
Flavobacterium columnare G
4, the transcripts of these three Ig genes exhibited quite different kinetics. Significantly increased transcription of IgM gene was observed in almost all the tissues examined especially in boosted group. In contrast with IgM, seldom strong increase was examined for IgD and IgZ genes. For IgD, it seemed that the first injection could stimulate the immune response easier, since in almost all the tissues significant increase was detected at 1 or 2 weeks after injection. For IgZ, boosted injection could not enlarge the up-regulation of gene expression of first injection. This is the first case to report the transcriptional kinetics of three Ig genes in teleost after bacterin immunization.</description><subject>Agnatha. Pisces</subject><subject>Animal aquaculture</subject><subject>animal pathogenic bacteria</subject><subject>Animal productions</subject><subject>Bacteria</subject><subject>bacterial infections</subject><subject>Biological and medical sciences</subject><subject>Fish</subject><subject>fish diseases</subject><subject>Flavobacterium columnare</subject><subject>Freshwater</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gene expression</subject><subject>gene expression regulation</subject><subject>General aspects</subject><subject>Genes</subject><subject>immune response</subject><subject>immunization</subject><subject>Immunoglobulin</subject><subject>immunoglobulin D</subject><subject>immunoglobulin M</subject><subject>immunoglobulin Z</subject><subject>Immunoglobulins</subject><subject>In situ hybridization</subject><subject>lymphoid tissue</subject><subject>Lymphoid tissues</subject><subject>Mandarin fish</subject><subject>Perciformes</subject><subject>Quantitive real-time PCR</subject><subject>Siniperca chuatsi</subject><subject>Sinipercidae</subject><subject>Teleostei</subject><subject>Tissues</subject><subject>transcription (genetics)</subject><subject>Vertebrates: general zoology, morphology, phylogeny, systematics, cytogenetics, geographical distribution</subject><issn>0044-8486</issn><issn>1873-5622</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2009</creationdate><recordtype>article</recordtype><recordid>eNqNkU1vEzEQhlcIJELhN2CQ4NQE22tvdo8opVCpiEPphYs18Y6TiXbXqT8q9e_wS_GSCiFOXGbm8Lzz9VbVG8FXgovmw2EFdxlsHlIOuJKctyshVlzWT6qFaNf1UjdSPq0WnCu1bFXbPK9exHjgnDeNFovq5wXFFGibE_mJeceudl_PS7hgMPUl_2A0sbHUEErhKO7P2Q1NdMRggdl9hhSJDQ_jce-pZ4lizBh_i9MeKbAUYIo20HEeAEORwLSbCZcwsMsB7v0WbKkpj8z6IY8TBGQx0ZgHmEUvq2cOhoivHvNZdXv56fvmy_L62-erzcfrpVV1nZZaoZVd16ETst1unW2VQtGAtM0aZa-3QqProe3RcmwdWKdqjUrXChWvXV-fVe9PfY_B35UjkhkpWhwGmNDnaCSvlZBrVcC3_4AHn0M5bmbUWrSt5gXqTpANPsaAzhwDjRAejOBmts4czF_Wmdk6I4Qp1hXtu8cBEC0MrrzQUvzTQJYtNF_rwr0-cQ68gV0ozO2N5KLmQney6bpCbE4ElsfdEwYTLeFksaeANpne03_s8wtjIsMC</recordid><startdate>20090302</startdate><enddate>20090302</enddate><creator>Tian, Jingyun</creator><creator>Sun, Baojian</creator><creator>Luo, Yanping</creator><creator>Zhang, Yongan</creator><creator>Nie, Pin</creator><general>Elsevier B.V</general><general>Amsterdam: Elsevier Science</general><general>Elsevier</general><general>Elsevier Sequoia S.A</general><scope>FBQ</scope><scope>IQODW</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7QR</scope><scope>7ST</scope><scope>7TN</scope><scope>7U7</scope><scope>7U9</scope><scope>8FD</scope><scope>C1K</scope><scope>F1W</scope><scope>FR3</scope><scope>H94</scope><scope>H95</scope><scope>H98</scope><scope>H99</scope><scope>L.F</scope><scope>L.G</scope><scope>M7N</scope><scope>P64</scope><scope>SOI</scope><scope>7T5</scope><scope>7T7</scope></search><sort><creationdate>20090302</creationdate><title>Distribution of IgM, IgD and IgZ in mandarin fish, Siniperca chuatsi lymphoid tissues and their transcriptional changes after Flavobacterium columnare stimulation</title><author>Tian, Jingyun ; Sun, Baojian ; Luo, Yanping ; Zhang, Yongan ; Nie, Pin</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c433t-54ec2999ef128bbfc844e16a2c67e2d5b15efda8dec0e8facf435e4534e403fd3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2009</creationdate><topic>Agnatha. Pisces</topic><topic>Animal aquaculture</topic><topic>animal pathogenic bacteria</topic><topic>Animal productions</topic><topic>Bacteria</topic><topic>bacterial infections</topic><topic>Biological and medical sciences</topic><topic>Fish</topic><topic>fish diseases</topic><topic>Flavobacterium columnare</topic><topic>Freshwater</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Gene expression</topic><topic>gene expression regulation</topic><topic>General aspects</topic><topic>Genes</topic><topic>immune response</topic><topic>immunization</topic><topic>Immunoglobulin</topic><topic>immunoglobulin D</topic><topic>immunoglobulin M</topic><topic>immunoglobulin Z</topic><topic>Immunoglobulins</topic><topic>In situ hybridization</topic><topic>lymphoid tissue</topic><topic>Lymphoid tissues</topic><topic>Mandarin fish</topic><topic>Perciformes</topic><topic>Quantitive real-time PCR</topic><topic>Siniperca chuatsi</topic><topic>Sinipercidae</topic><topic>Teleostei</topic><topic>Tissues</topic><topic>transcription (genetics)</topic><topic>Vertebrates: general zoology, morphology, phylogeny, systematics, cytogenetics, geographical distribution</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Tian, Jingyun</creatorcontrib><creatorcontrib>Sun, Baojian</creatorcontrib><creatorcontrib>Luo, Yanping</creatorcontrib><creatorcontrib>Zhang, Yongan</creatorcontrib><creatorcontrib>Nie, Pin</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Chemoreception Abstracts</collection><collection>Environment Abstracts</collection><collection>Oceanic Abstracts</collection><collection>Toxicology Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ASFA: Aquatic Sciences and Fisheries Abstracts</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) 1: Biological Sciences & Living Resources</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Aquaculture Abstracts</collection><collection>ASFA: Marine Biotechnology Abstracts</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Marine Biotechnology Abstracts</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Professional</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Environment Abstracts</collection><collection>Immunology Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><jtitle>Aquaculture</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Tian, Jingyun</au><au>Sun, Baojian</au><au>Luo, Yanping</au><au>Zhang, Yongan</au><au>Nie, Pin</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Distribution of IgM, IgD and IgZ in mandarin fish, Siniperca chuatsi lymphoid tissues and their transcriptional changes after Flavobacterium columnare stimulation</atitle><jtitle>Aquaculture</jtitle><date>2009-03-02</date><risdate>2009</risdate><volume>288</volume><issue>1</issue><spage>14</spage><epage>21</epage><pages>14-21</pages><issn>0044-8486</issn><eissn>1873-5622</eissn><coden>AQCLAL</coden><abstract>The gene sequences of three different immunoglobulin (Ig) heavy chains, namely IgM, IgD and IgZ, were cloned from mandarin fish (
Siniperca chuatsi) recently. In this study the distribution of these three kinds of Ig-producing cells in lymphoid-related tissues as head kidney, spleen, gill and intestine were investigated by using
in situ hybridization, and their transcriptional changes were also analyzed by quantitative real-time PCR during 8 weeks after immunization. IgM-producing cells could be detected obviously and abundantly in all the tissues examined. A few numbers of IgD and IgZ positive cells were both detected in head kidney and spleen. IgZ positive cells could be detected in gill moderately while IgD showed negative results, otherwise no IgD or IgZ positive cells could be detected in intestine. After stimulated with bacterial pathogen
Flavobacterium columnare G
4, the transcripts of these three Ig genes exhibited quite different kinetics. Significantly increased transcription of IgM gene was observed in almost all the tissues examined especially in boosted group. In contrast with IgM, seldom strong increase was examined for IgD and IgZ genes. For IgD, it seemed that the first injection could stimulate the immune response easier, since in almost all the tissues significant increase was detected at 1 or 2 weeks after injection. For IgZ, boosted injection could not enlarge the up-regulation of gene expression of first injection. This is the first case to report the transcriptional kinetics of three Ig genes in teleost after bacterin immunization.</abstract><cop>Amsterdam</cop><pub>Elsevier B.V</pub><doi>10.1016/j.aquaculture.2008.11.023</doi><tpages>8</tpages></addata></record> |
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| subjects | Agnatha. Pisces Animal aquaculture animal pathogenic bacteria Animal productions Bacteria bacterial infections Biological and medical sciences Fish fish diseases Flavobacterium columnare Freshwater Fundamental and applied biological sciences. Psychology Gene expression gene expression regulation General aspects Genes immune response immunization Immunoglobulin immunoglobulin D immunoglobulin M immunoglobulin Z Immunoglobulins In situ hybridization lymphoid tissue Lymphoid tissues Mandarin fish Perciformes Quantitive real-time PCR Siniperca chuatsi Sinipercidae Teleostei Tissues transcription (genetics) Vertebrates: general zoology, morphology, phylogeny, systematics, cytogenetics, geographical distribution |
| title | Distribution of IgM, IgD and IgZ in mandarin fish, Siniperca chuatsi lymphoid tissues and their transcriptional changes after Flavobacterium columnare stimulation |
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