Electrochemical immunosensor for differential diagnostic of Wuchereria bancrofti using a synthetic peptide

Lymphatic filariasis (LF) is a neglected tropical disease transmitted by mosquitoes and the second cause of permanent disability leading to a significant morbidity and mortality rate. Previously, we have identified epitopes of the filarial abundant larval transcript-2 (ALT-2) protein using a microar...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Veröffentlicht in:Biosensors & bioelectronics 2018-08, Vol.113, p.9-15
Hauptverfasser: Prado, Isis C., Mendes, Verônica G., Souza, André L.A., Dutra, Rosa F., De-Simone, Salvatore G.
Format: Artikel
Sprache:eng
Schlagworte:
Online-Zugang:Volltext
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
container_end_page 15
container_issue
container_start_page 9
container_title Biosensors & bioelectronics
container_volume 113
creator Prado, Isis C.
Mendes, Verônica G.
Souza, André L.A.
Dutra, Rosa F.
De-Simone, Salvatore G.
description Lymphatic filariasis (LF) is a neglected tropical disease transmitted by mosquitoes and the second cause of permanent disability leading to a significant morbidity and mortality rate. Previously, we have identified epitopes of the filarial abundant larval transcript-2 (ALT-2) protein using a microarray mapping. In this study, one of the epitopes (Wb/ALT2-A5) was used to construct an electrochemical immunosensor. Electrochemical technique of cyclic voltammetry was performed for detecting the signal generated by the interaction between the (Wb/ALT2-A5) peptide and circulating antibodies of serum human samples. (Wb/ALT2-A5) epitope antigens were successfully immobilized on the working electrode of a screen-printed carbon electrode (SPCE) by their amine groups via chitosan film by coupling with glutaraldehyde as crosslinker. After the sensor ready, a pool of human sera infected with Wuchereria bancrofti was added to its surface. Electrochemical responses were generated by applying a potential of − 0.6 to 0.6 V, scan rate of 0.025 V/s. A detection limit of 5.0 µg mL−1 for the synthetic peptides (Wb/ALT2-A5) and 0.002 µg mL−1 for human serum, with a sensitivity of 1.86 µA. The performance of this assay was successfully tested in human serum samples from infected and healthy patients. Thus, this proposed immunosensor, which is able to identify circulating antibodies, can be applied to the diagnosis of the W. bancrofti parasitic disease. •The lymphatic filariasis (LF) is a parasitic infection, exclusive to humans.•In endemic countries, about 120 million individuals are infected by this helminth.•The diagnosis is performed by parasitological, immunological techniques among others.•The mapping of epitopes of the ALT-2 from the worm Wuchereria bancrofti was carried.•The immunosensor was capable of identifying circulating antibodies in human sera.
doi_str_mv 10.1016/j.bios.2018.04.045
format Article
fullrecord <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_2033384219</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>S0956566318303063</els_id><sourcerecordid>2033384219</sourcerecordid><originalsourceid>FETCH-LOGICAL-c356t-c970bf6c6d91397194b15aaf0d1c3cb61a8d02401f2926364a9a26beea88dead3</originalsourceid><addsrcrecordid>eNp9kEtLxDAUhYMoOj7-gAvp0k3HPNq0ATcivkBwo7gMaXKjd2ibMWkF_70ZRl0KN2Rxzzmc-xFyyuiSUSYvVssOQ1pyytolrfLUO2TB2kaUFRf1LllQVcuyllIckMOUVpTShim6Tw64aqhqmnZBVjc92CkG-w4DWtMXOAzzGBKMKcTC5-fQe4gwTpi3Ds1b3k5oi-CL1znbIkQ0RWdGG4OfsJgTjm-FKdLXOL3DRrmG9YQOjsmeN32Ck5__iLzc3jxf35ePT3cP11ePpRW1nEqbu3VeWukUEyoXrjpWG-OpY1bYTjLTOsoryjxXXApZGWW47ABM2zowThyR823uOoaPGdKkB0wW-t6MEOakORVCtBVnKkv5Vpq7pxTB63XEwcQvzajeMNYrvWGsN4w1rfLU2XT2kz93A7g_yy_ULLjcCiBf-YkQdbIIowWHMcPWLuB_-d8It5At</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2033384219</pqid></control><display><type>article</type><title>Electrochemical immunosensor for differential diagnostic of Wuchereria bancrofti using a synthetic peptide</title><source>Elsevier ScienceDirect Journals Complete</source><creator>Prado, Isis C. ; Mendes, Verônica G. ; Souza, André L.A. ; Dutra, Rosa F. ; De-Simone, Salvatore G.</creator><creatorcontrib>Prado, Isis C. ; Mendes, Verônica G. ; Souza, André L.A. ; Dutra, Rosa F. ; De-Simone, Salvatore G.</creatorcontrib><description>Lymphatic filariasis (LF) is a neglected tropical disease transmitted by mosquitoes and the second cause of permanent disability leading to a significant morbidity and mortality rate. Previously, we have identified epitopes of the filarial abundant larval transcript-2 (ALT-2) protein using a microarray mapping. In this study, one of the epitopes (Wb/ALT2-A5) was used to construct an electrochemical immunosensor. Electrochemical technique of cyclic voltammetry was performed for detecting the signal generated by the interaction between the (Wb/ALT2-A5) peptide and circulating antibodies of serum human samples. (Wb/ALT2-A5) epitope antigens were successfully immobilized on the working electrode of a screen-printed carbon electrode (SPCE) by their amine groups via chitosan film by coupling with glutaraldehyde as crosslinker. After the sensor ready, a pool of human sera infected with Wuchereria bancrofti was added to its surface. Electrochemical responses were generated by applying a potential of − 0.6 to 0.6 V, scan rate of 0.025 V/s. A detection limit of 5.0 µg mL−1 for the synthetic peptides (Wb/ALT2-A5) and 0.002 µg mL−1 for human serum, with a sensitivity of 1.86 µA. The performance of this assay was successfully tested in human serum samples from infected and healthy patients. Thus, this proposed immunosensor, which is able to identify circulating antibodies, can be applied to the diagnosis of the W. bancrofti parasitic disease. •The lymphatic filariasis (LF) is a parasitic infection, exclusive to humans.•In endemic countries, about 120 million individuals are infected by this helminth.•The diagnosis is performed by parasitological, immunological techniques among others.•The mapping of epitopes of the ALT-2 from the worm Wuchereria bancrofti was carried.•The immunosensor was capable of identifying circulating antibodies in human sera.</description><identifier>ISSN: 0956-5663</identifier><identifier>EISSN: 1873-4235</identifier><identifier>DOI: 10.1016/j.bios.2018.04.045</identifier><identifier>PMID: 29709778</identifier><language>eng</language><publisher>England: Elsevier B.V</publisher><subject>Diagnostic ; Electroanalytic ; Electroanalytical immunoassay ; Epitope ; Wuchereria bancrofti</subject><ispartof>Biosensors &amp; bioelectronics, 2018-08, Vol.113, p.9-15</ispartof><rights>2018 Elsevier B.V.</rights><rights>Copyright © 2018 Elsevier B.V. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c356t-c970bf6c6d91397194b15aaf0d1c3cb61a8d02401f2926364a9a26beea88dead3</citedby><cites>FETCH-LOGICAL-c356t-c970bf6c6d91397194b15aaf0d1c3cb61a8d02401f2926364a9a26beea88dead3</cites><orcidid>0000-0001-8314-4218 ; 0000-0002-4860-4642</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.bios.2018.04.045$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/29709778$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Prado, Isis C.</creatorcontrib><creatorcontrib>Mendes, Verônica G.</creatorcontrib><creatorcontrib>Souza, André L.A.</creatorcontrib><creatorcontrib>Dutra, Rosa F.</creatorcontrib><creatorcontrib>De-Simone, Salvatore G.</creatorcontrib><title>Electrochemical immunosensor for differential diagnostic of Wuchereria bancrofti using a synthetic peptide</title><title>Biosensors &amp; bioelectronics</title><addtitle>Biosens Bioelectron</addtitle><description>Lymphatic filariasis (LF) is a neglected tropical disease transmitted by mosquitoes and the second cause of permanent disability leading to a significant morbidity and mortality rate. Previously, we have identified epitopes of the filarial abundant larval transcript-2 (ALT-2) protein using a microarray mapping. In this study, one of the epitopes (Wb/ALT2-A5) was used to construct an electrochemical immunosensor. Electrochemical technique of cyclic voltammetry was performed for detecting the signal generated by the interaction between the (Wb/ALT2-A5) peptide and circulating antibodies of serum human samples. (Wb/ALT2-A5) epitope antigens were successfully immobilized on the working electrode of a screen-printed carbon electrode (SPCE) by their amine groups via chitosan film by coupling with glutaraldehyde as crosslinker. After the sensor ready, a pool of human sera infected with Wuchereria bancrofti was added to its surface. Electrochemical responses were generated by applying a potential of − 0.6 to 0.6 V, scan rate of 0.025 V/s. A detection limit of 5.0 µg mL−1 for the synthetic peptides (Wb/ALT2-A5) and 0.002 µg mL−1 for human serum, with a sensitivity of 1.86 µA. The performance of this assay was successfully tested in human serum samples from infected and healthy patients. Thus, this proposed immunosensor, which is able to identify circulating antibodies, can be applied to the diagnosis of the W. bancrofti parasitic disease. •The lymphatic filariasis (LF) is a parasitic infection, exclusive to humans.•In endemic countries, about 120 million individuals are infected by this helminth.•The diagnosis is performed by parasitological, immunological techniques among others.•The mapping of epitopes of the ALT-2 from the worm Wuchereria bancrofti was carried.•The immunosensor was capable of identifying circulating antibodies in human sera.</description><subject>Diagnostic</subject><subject>Electroanalytic</subject><subject>Electroanalytical immunoassay</subject><subject>Epitope</subject><subject>Wuchereria bancrofti</subject><issn>0956-5663</issn><issn>1873-4235</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2018</creationdate><recordtype>article</recordtype><recordid>eNp9kEtLxDAUhYMoOj7-gAvp0k3HPNq0ATcivkBwo7gMaXKjd2ibMWkF_70ZRl0KN2Rxzzmc-xFyyuiSUSYvVssOQ1pyytolrfLUO2TB2kaUFRf1LllQVcuyllIckMOUVpTShim6Tw64aqhqmnZBVjc92CkG-w4DWtMXOAzzGBKMKcTC5-fQe4gwTpi3Ds1b3k5oi-CL1znbIkQ0RWdGG4OfsJgTjm-FKdLXOL3DRrmG9YQOjsmeN32Ck5__iLzc3jxf35ePT3cP11ePpRW1nEqbu3VeWukUEyoXrjpWG-OpY1bYTjLTOsoryjxXXApZGWW47ABM2zowThyR823uOoaPGdKkB0wW-t6MEOakORVCtBVnKkv5Vpq7pxTB63XEwcQvzajeMNYrvWGsN4w1rfLU2XT2kz93A7g_yy_ULLjcCiBf-YkQdbIIowWHMcPWLuB_-d8It5At</recordid><startdate>20180815</startdate><enddate>20180815</enddate><creator>Prado, Isis C.</creator><creator>Mendes, Verônica G.</creator><creator>Souza, André L.A.</creator><creator>Dutra, Rosa F.</creator><creator>De-Simone, Salvatore G.</creator><general>Elsevier B.V</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0001-8314-4218</orcidid><orcidid>https://orcid.org/0000-0002-4860-4642</orcidid></search><sort><creationdate>20180815</creationdate><title>Electrochemical immunosensor for differential diagnostic of Wuchereria bancrofti using a synthetic peptide</title><author>Prado, Isis C. ; Mendes, Verônica G. ; Souza, André L.A. ; Dutra, Rosa F. ; De-Simone, Salvatore G.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c356t-c970bf6c6d91397194b15aaf0d1c3cb61a8d02401f2926364a9a26beea88dead3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2018</creationdate><topic>Diagnostic</topic><topic>Electroanalytic</topic><topic>Electroanalytical immunoassay</topic><topic>Epitope</topic><topic>Wuchereria bancrofti</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Prado, Isis C.</creatorcontrib><creatorcontrib>Mendes, Verônica G.</creatorcontrib><creatorcontrib>Souza, André L.A.</creatorcontrib><creatorcontrib>Dutra, Rosa F.</creatorcontrib><creatorcontrib>De-Simone, Salvatore G.</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Biosensors &amp; bioelectronics</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Prado, Isis C.</au><au>Mendes, Verônica G.</au><au>Souza, André L.A.</au><au>Dutra, Rosa F.</au><au>De-Simone, Salvatore G.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Electrochemical immunosensor for differential diagnostic of Wuchereria bancrofti using a synthetic peptide</atitle><jtitle>Biosensors &amp; bioelectronics</jtitle><addtitle>Biosens Bioelectron</addtitle><date>2018-08-15</date><risdate>2018</risdate><volume>113</volume><spage>9</spage><epage>15</epage><pages>9-15</pages><issn>0956-5663</issn><eissn>1873-4235</eissn><abstract>Lymphatic filariasis (LF) is a neglected tropical disease transmitted by mosquitoes and the second cause of permanent disability leading to a significant morbidity and mortality rate. Previously, we have identified epitopes of the filarial abundant larval transcript-2 (ALT-2) protein using a microarray mapping. In this study, one of the epitopes (Wb/ALT2-A5) was used to construct an electrochemical immunosensor. Electrochemical technique of cyclic voltammetry was performed for detecting the signal generated by the interaction between the (Wb/ALT2-A5) peptide and circulating antibodies of serum human samples. (Wb/ALT2-A5) epitope antigens were successfully immobilized on the working electrode of a screen-printed carbon electrode (SPCE) by their amine groups via chitosan film by coupling with glutaraldehyde as crosslinker. After the sensor ready, a pool of human sera infected with Wuchereria bancrofti was added to its surface. Electrochemical responses were generated by applying a potential of − 0.6 to 0.6 V, scan rate of 0.025 V/s. A detection limit of 5.0 µg mL−1 for the synthetic peptides (Wb/ALT2-A5) and 0.002 µg mL−1 for human serum, with a sensitivity of 1.86 µA. The performance of this assay was successfully tested in human serum samples from infected and healthy patients. Thus, this proposed immunosensor, which is able to identify circulating antibodies, can be applied to the diagnosis of the W. bancrofti parasitic disease. •The lymphatic filariasis (LF) is a parasitic infection, exclusive to humans.•In endemic countries, about 120 million individuals are infected by this helminth.•The diagnosis is performed by parasitological, immunological techniques among others.•The mapping of epitopes of the ALT-2 from the worm Wuchereria bancrofti was carried.•The immunosensor was capable of identifying circulating antibodies in human sera.</abstract><cop>England</cop><pub>Elsevier B.V</pub><pmid>29709778</pmid><doi>10.1016/j.bios.2018.04.045</doi><tpages>7</tpages><orcidid>https://orcid.org/0000-0001-8314-4218</orcidid><orcidid>https://orcid.org/0000-0002-4860-4642</orcidid></addata></record>
fulltext fulltext
identifier ISSN: 0956-5663
ispartof Biosensors & bioelectronics, 2018-08, Vol.113, p.9-15
issn 0956-5663
1873-4235
language eng
recordid cdi_proquest_miscellaneous_2033384219
source Elsevier ScienceDirect Journals Complete
subjects Diagnostic
Electroanalytic
Electroanalytical immunoassay
Epitope
Wuchereria bancrofti
title Electrochemical immunosensor for differential diagnostic of Wuchereria bancrofti using a synthetic peptide
url https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-25T20%3A22%3A45IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Electrochemical%20immunosensor%20for%20differential%20diagnostic%20of%20Wuchereria%20bancrofti%20using%20a%20synthetic%20peptide&rft.jtitle=Biosensors%20&%20bioelectronics&rft.au=Prado,%20Isis%20C.&rft.date=2018-08-15&rft.volume=113&rft.spage=9&rft.epage=15&rft.pages=9-15&rft.issn=0956-5663&rft.eissn=1873-4235&rft_id=info:doi/10.1016/j.bios.2018.04.045&rft_dat=%3Cproquest_cross%3E2033384219%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2033384219&rft_id=info:pmid/29709778&rft_els_id=S0956566318303063&rfr_iscdi=true