Allogeneic mesenchymal stem cell transplantation in healthy equine superficial digital flexor tendon: A study of the local inflammatory response
The superficial digital flexor tendon (SDFT) is a structure frequently affected by injuries in high-performance athletic horses, and there are limited therapeutic options. Regenerative medicine has evolved significantly in treating different illnesses. However, understanding the cellular behaviour d...
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| Veröffentlicht in: | Research in veterinary science 2018-06, Vol.118, p.423-430 |
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| creator | Brandão, Jaqueline Souza Alvarenga, Marina Landim Pfeifer, João Pedro Hubbe dos Santos, Vitor Hugo Fonseca-Alves, Carlos Eduardo Rodrigues, Mirian Laufer-Amorim, Renée Castillo, José Antonio Lucas Alves, Ana Liz Garcia |
| description | The superficial digital flexor tendon (SDFT) is a structure frequently affected by injuries in high-performance athletic horses, and there are limited therapeutic options. Regenerative medicine has evolved significantly in treating different illnesses. However, understanding the cellular behaviour during mesenchymal stem cell (MSC) transplantation in healthy tissues is not fully known yet. To address the inflammatory response induced by allogeneic MSC transplantation, this study evaluated the local inflammatory response after the application of allogeneic adipose tissue-derived mesenchymal stem cells (AT-MSCs) in the equine tendon compared to an autologous transplant and the control group. Eighteen thoracic limbs (TL) in nine animals were divided into three groups and subjected to the application of AT-MSCs in the healthy tendon. In the allogeneic group (Gallog), the animals received an allogeneic AT-MSC application in the TL. The autologous group (Gauto) received an application of autologous cells in the TL, and in the control group (Gcont), phosphate-buffered saline (PBS) was applied. There were no significant differences among the evaluated groups in the physical, morphological, thermography, and ultrasonography analyses. A higher number of CD3-positive lymphocytes was observed in the Gauto group compared to the control (P |
| doi_str_mv | 10.1016/j.rvsc.2018.03.012 |
| format | Article |
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•Allogeneic mesenchymal stem cell transplantation do not induce deleterious inflammatory response.•The allogeneic cell banks can be used to treatment of equine disorders.•Allogeneic stem cell transplantation do not stimulate neovascularization.</description><identifier>ISSN: 0034-5288</identifier><identifier>EISSN: 1532-2661</identifier><identifier>DOI: 10.1016/j.rvsc.2018.03.012</identifier><identifier>PMID: 29715649</identifier><language>eng</language><publisher>England: Elsevier Ltd</publisher><subject>Adipose tissue ; Allogeneic ; Animals ; Autografts ; CD3 antigen ; Equine ; Growth factors ; Horse Diseases - surgery ; Horses ; Illnesses ; Immunologic response ; Inflammation ; Inflammatory response ; Injury prevention ; Lymphocytes ; Mesenchymal Stem Cell Transplantation ; Mesenchymal Stem Cells ; Mesenchyme ; Microvasculature ; Regenerative medicine ; Standard deviation ; Stem cell transplantation ; Stem cells ; Studies ; Tendinitis ; Tendons ; Thermography ; Thorax ; Tissue engineering ; Transplantation ; Transplantation, Autologous ; Transplantation, Homologous ; Ultrasound ; Variance analysis ; Veterinary medicine</subject><ispartof>Research in veterinary science, 2018-06, Vol.118, p.423-430</ispartof><rights>2018</rights><rights>Copyright © 2018. Published by Elsevier Ltd.</rights><rights>Copyright Elsevier Limited Jun 2018</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c428t-8a6eea6fc46d0f41622f01479606a97c7766415132b59a367f58ade6d0766e893</citedby><cites>FETCH-LOGICAL-c428t-8a6eea6fc46d0f41622f01479606a97c7766415132b59a367f58ade6d0766e893</cites><orcidid>0000-0001-9092-7819 ; 0000-0002-6702-6139</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.rvsc.2018.03.012$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>315,781,785,3551,27929,27930,46000</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/29715649$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Brandão, Jaqueline Souza</creatorcontrib><creatorcontrib>Alvarenga, Marina Landim</creatorcontrib><creatorcontrib>Pfeifer, João Pedro Hubbe</creatorcontrib><creatorcontrib>dos Santos, Vitor Hugo</creatorcontrib><creatorcontrib>Fonseca-Alves, Carlos Eduardo</creatorcontrib><creatorcontrib>Rodrigues, Mirian</creatorcontrib><creatorcontrib>Laufer-Amorim, Renée</creatorcontrib><creatorcontrib>Castillo, José Antonio Lucas</creatorcontrib><creatorcontrib>Alves, Ana Liz Garcia</creatorcontrib><title>Allogeneic mesenchymal stem cell transplantation in healthy equine superficial digital flexor tendon: A study of the local inflammatory response</title><title>Research in veterinary science</title><addtitle>Res Vet Sci</addtitle><description>The superficial digital flexor tendon (SDFT) is a structure frequently affected by injuries in high-performance athletic horses, and there are limited therapeutic options. Regenerative medicine has evolved significantly in treating different illnesses. However, understanding the cellular behaviour during mesenchymal stem cell (MSC) transplantation in healthy tissues is not fully known yet. To address the inflammatory response induced by allogeneic MSC transplantation, this study evaluated the local inflammatory response after the application of allogeneic adipose tissue-derived mesenchymal stem cells (AT-MSCs) in the equine tendon compared to an autologous transplant and the control group. Eighteen thoracic limbs (TL) in nine animals were divided into three groups and subjected to the application of AT-MSCs in the healthy tendon. In the allogeneic group (Gallog), the animals received an allogeneic AT-MSC application in the TL. The autologous group (Gauto) received an application of autologous cells in the TL, and in the control group (Gcont), phosphate-buffered saline (PBS) was applied. There were no significant differences among the evaluated groups in the physical, morphological, thermography, and ultrasonography analyses. A higher number of CD3-positive lymphocytes was observed in the Gauto group compared to the control (P < 0.05). Additionally, we did not observe different expressions of CD172 and microvascular density among the groups. The allogeneic transplantation of AT-MSCs did not result in an adverse or inflammatory reaction that compromised the use of these cells in this experiment. Their behaviour was similar to that of autologous transplantation.
•Allogeneic mesenchymal stem cell transplantation do not induce deleterious inflammatory response.•The allogeneic cell banks can be used to treatment of equine disorders.•Allogeneic stem cell transplantation do not stimulate neovascularization.</description><subject>Adipose tissue</subject><subject>Allogeneic</subject><subject>Animals</subject><subject>Autografts</subject><subject>CD3 antigen</subject><subject>Equine</subject><subject>Growth factors</subject><subject>Horse Diseases - surgery</subject><subject>Horses</subject><subject>Illnesses</subject><subject>Immunologic response</subject><subject>Inflammation</subject><subject>Inflammatory response</subject><subject>Injury prevention</subject><subject>Lymphocytes</subject><subject>Mesenchymal Stem Cell Transplantation</subject><subject>Mesenchymal Stem Cells</subject><subject>Mesenchyme</subject><subject>Microvasculature</subject><subject>Regenerative medicine</subject><subject>Standard deviation</subject><subject>Stem cell transplantation</subject><subject>Stem cells</subject><subject>Studies</subject><subject>Tendinitis</subject><subject>Tendons</subject><subject>Thermography</subject><subject>Thorax</subject><subject>Tissue engineering</subject><subject>Transplantation</subject><subject>Transplantation, Autologous</subject><subject>Transplantation, Homologous</subject><subject>Ultrasound</subject><subject>Variance analysis</subject><subject>Veterinary medicine</subject><issn>0034-5288</issn><issn>1532-2661</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2018</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kctu1DAUhi0EokPhBVggS2zYJPiS2AliM6q4SZXYwNpyneOOR46d2k7VvAWPjEdTWLBgdRb-_l_n-EPoNSUtJVS8P7bpPpuWETq0hLeEsidoR3vOGiYEfYp2hPCu6dkwXKAXOR8JIR2l8jm6YKOkvejGHfq19z7eQgBn8AwZgjlss_Y4F5ixAe9xSTrkxetQdHExYBfwAbQvhw3D3eoC4LwukKwzruYmd-tKndbDQ0y4QJhi-ID3tXCdNhwtLgfAPprKuGC9nmddYtpwgrzEkOElema1z_DqcV6in58__bj62lx___Ltan_dmI4NpRm0ANDCmk5MxHZUMGYJ7eQoiNCjNFIK0dGecnbTj5oLaftBT1Dh-gDDyC_Ru3PvkuLdCrmo2eXTwTpAXLNihHMu6SBpRd_-gx7jmkLdrlIdp4wLIivFzpRJMecEVi3JzTptihJ18qWO6uRLnXwpwlX1VUNvHqvXmxmmv5E_girw8QxA_Yt7B0ll46olmFwCU9QU3f_6fwOFx6it</recordid><startdate>201806</startdate><enddate>201806</enddate><creator>Brandão, Jaqueline Souza</creator><creator>Alvarenga, Marina Landim</creator><creator>Pfeifer, João Pedro Hubbe</creator><creator>dos Santos, Vitor Hugo</creator><creator>Fonseca-Alves, Carlos Eduardo</creator><creator>Rodrigues, Mirian</creator><creator>Laufer-Amorim, Renée</creator><creator>Castillo, José Antonio Lucas</creator><creator>Alves, Ana Liz Garcia</creator><general>Elsevier Ltd</general><general>Elsevier Limited</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QG</scope><scope>7QP</scope><scope>7QR</scope><scope>7T5</scope><scope>7T7</scope><scope>7TK</scope><scope>7TM</scope><scope>7TO</scope><scope>7U7</scope><scope>7U9</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>H94</scope><scope>K9.</scope><scope>M7N</scope><scope>P64</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0001-9092-7819</orcidid><orcidid>https://orcid.org/0000-0002-6702-6139</orcidid></search><sort><creationdate>201806</creationdate><title>Allogeneic mesenchymal stem cell transplantation in healthy equine superficial digital flexor tendon: A study of the local inflammatory response</title><author>Brandão, Jaqueline Souza ; Alvarenga, Marina Landim ; Pfeifer, João Pedro Hubbe ; dos Santos, Vitor Hugo ; Fonseca-Alves, Carlos Eduardo ; Rodrigues, Mirian ; Laufer-Amorim, Renée ; Castillo, José Antonio Lucas ; Alves, Ana Liz Garcia</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c428t-8a6eea6fc46d0f41622f01479606a97c7766415132b59a367f58ade6d0766e893</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2018</creationdate><topic>Adipose tissue</topic><topic>Allogeneic</topic><topic>Animals</topic><topic>Autografts</topic><topic>CD3 antigen</topic><topic>Equine</topic><topic>Growth factors</topic><topic>Horse Diseases - surgery</topic><topic>Horses</topic><topic>Illnesses</topic><topic>Immunologic response</topic><topic>Inflammation</topic><topic>Inflammatory response</topic><topic>Injury prevention</topic><topic>Lymphocytes</topic><topic>Mesenchymal Stem Cell Transplantation</topic><topic>Mesenchymal Stem Cells</topic><topic>Mesenchyme</topic><topic>Microvasculature</topic><topic>Regenerative medicine</topic><topic>Standard deviation</topic><topic>Stem cell transplantation</topic><topic>Stem cells</topic><topic>Studies</topic><topic>Tendinitis</topic><topic>Tendons</topic><topic>Thermography</topic><topic>Thorax</topic><topic>Tissue engineering</topic><topic>Transplantation</topic><topic>Transplantation, Autologous</topic><topic>Transplantation, Homologous</topic><topic>Ultrasound</topic><topic>Variance analysis</topic><topic>Veterinary medicine</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Brandão, Jaqueline Souza</creatorcontrib><creatorcontrib>Alvarenga, Marina Landim</creatorcontrib><creatorcontrib>Pfeifer, João Pedro Hubbe</creatorcontrib><creatorcontrib>dos Santos, Vitor Hugo</creatorcontrib><creatorcontrib>Fonseca-Alves, Carlos Eduardo</creatorcontrib><creatorcontrib>Rodrigues, Mirian</creatorcontrib><creatorcontrib>Laufer-Amorim, Renée</creatorcontrib><creatorcontrib>Castillo, José Antonio Lucas</creatorcontrib><creatorcontrib>Alves, Ana Liz Garcia</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Animal Behavior Abstracts</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Chemoreception Abstracts</collection><collection>Immunology Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Neurosciences Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Oncogenes and Growth Factors Abstracts</collection><collection>Toxicology Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Research in veterinary science</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Brandão, Jaqueline Souza</au><au>Alvarenga, Marina Landim</au><au>Pfeifer, João Pedro Hubbe</au><au>dos Santos, Vitor Hugo</au><au>Fonseca-Alves, Carlos Eduardo</au><au>Rodrigues, Mirian</au><au>Laufer-Amorim, Renée</au><au>Castillo, José Antonio Lucas</au><au>Alves, Ana Liz Garcia</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Allogeneic mesenchymal stem cell transplantation in healthy equine superficial digital flexor tendon: A study of the local inflammatory response</atitle><jtitle>Research in veterinary science</jtitle><addtitle>Res Vet Sci</addtitle><date>2018-06</date><risdate>2018</risdate><volume>118</volume><spage>423</spage><epage>430</epage><pages>423-430</pages><issn>0034-5288</issn><eissn>1532-2661</eissn><abstract>The superficial digital flexor tendon (SDFT) is a structure frequently affected by injuries in high-performance athletic horses, and there are limited therapeutic options. Regenerative medicine has evolved significantly in treating different illnesses. However, understanding the cellular behaviour during mesenchymal stem cell (MSC) transplantation in healthy tissues is not fully known yet. To address the inflammatory response induced by allogeneic MSC transplantation, this study evaluated the local inflammatory response after the application of allogeneic adipose tissue-derived mesenchymal stem cells (AT-MSCs) in the equine tendon compared to an autologous transplant and the control group. Eighteen thoracic limbs (TL) in nine animals were divided into three groups and subjected to the application of AT-MSCs in the healthy tendon. In the allogeneic group (Gallog), the animals received an allogeneic AT-MSC application in the TL. The autologous group (Gauto) received an application of autologous cells in the TL, and in the control group (Gcont), phosphate-buffered saline (PBS) was applied. There were no significant differences among the evaluated groups in the physical, morphological, thermography, and ultrasonography analyses. A higher number of CD3-positive lymphocytes was observed in the Gauto group compared to the control (P < 0.05). Additionally, we did not observe different expressions of CD172 and microvascular density among the groups. The allogeneic transplantation of AT-MSCs did not result in an adverse or inflammatory reaction that compromised the use of these cells in this experiment. Their behaviour was similar to that of autologous transplantation.
•Allogeneic mesenchymal stem cell transplantation do not induce deleterious inflammatory response.•The allogeneic cell banks can be used to treatment of equine disorders.•Allogeneic stem cell transplantation do not stimulate neovascularization.</abstract><cop>England</cop><pub>Elsevier Ltd</pub><pmid>29715649</pmid><doi>10.1016/j.rvsc.2018.03.012</doi><tpages>8</tpages><orcidid>https://orcid.org/0000-0001-9092-7819</orcidid><orcidid>https://orcid.org/0000-0002-6702-6139</orcidid><oa>free_for_read</oa></addata></record> |
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| subjects | Adipose tissue Allogeneic Animals Autografts CD3 antigen Equine Growth factors Horse Diseases - surgery Horses Illnesses Immunologic response Inflammation Inflammatory response Injury prevention Lymphocytes Mesenchymal Stem Cell Transplantation Mesenchymal Stem Cells Mesenchyme Microvasculature Regenerative medicine Standard deviation Stem cell transplantation Stem cells Studies Tendinitis Tendons Thermography Thorax Tissue engineering Transplantation Transplantation, Autologous Transplantation, Homologous Ultrasound Variance analysis Veterinary medicine |
| title | Allogeneic mesenchymal stem cell transplantation in healthy equine superficial digital flexor tendon: A study of the local inflammatory response |
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