Effects of the deficiency of the rhodanese-like protein RhdA in Azotobacter vinelandii
In Azotobacter vinelandii the rhdA gene codes for a protein (RhdA) of the rhodanese-homology superfamily. By combining proteomics, enzymic profiles and ultrastructural observations, the phenotype of an A. vinelandii rhdA mutant was analyzed. We found that the A. vinelandii rhdA mutant, and not the w...
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| Veröffentlicht in: | FEBS letters 2007-04, Vol.581 (8), p.1625-1630 |
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| creator | Cereda, Angelo Carpen, Aristodemo Picariello, Gianluca Iriti, Marcello Faoro, Franco Ferranti, Pasquale Pagani, Silvia |
| description | In
Azotobacter vinelandii the
rhdA gene codes for a protein (RhdA) of the rhodanese-homology superfamily. By combining proteomics, enzymic profiles and ultrastructural observations, the phenotype of an
A. vinelandii rhdA mutant was analyzed. We found that the
A. vinelandii rhdA mutant, and not the wild-type strain, accumulated polyhydroxybutyrate. RhdA deficiency enhanced the expression of enzymes of the polyhydroxybutyrate biosynthetic operon, and affected the activity of specific tricarboxylic acid cycle enzymes. The effect was dramatic on aconitase, in spite of comparable expression of aconitase polypeptides in both strains. By using a model system, we found that RhdA triggered protection from oxidants. |
| doi_str_mv | 10.1016/j.febslet.2007.03.028 |
| format | Article |
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Azotobacter vinelandii the
rhdA gene codes for a protein (RhdA) of the rhodanese-homology superfamily. By combining proteomics, enzymic profiles and ultrastructural observations, the phenotype of an
A. vinelandii rhdA mutant was analyzed. We found that the
A. vinelandii rhdA mutant, and not the wild-type strain, accumulated polyhydroxybutyrate. RhdA deficiency enhanced the expression of enzymes of the polyhydroxybutyrate biosynthetic operon, and affected the activity of specific tricarboxylic acid cycle enzymes. The effect was dramatic on aconitase, in spite of comparable expression of aconitase polypeptides in both strains. By using a model system, we found that RhdA triggered protection from oxidants.</description><identifier>ISSN: 0014-5793</identifier><identifier>EISSN: 1873-3468</identifier><identifier>DOI: 10.1016/j.febslet.2007.03.028</identifier><identifier>PMID: 17383639</identifier><language>eng</language><publisher>England: Elsevier B.V</publisher><subject>Azotobacter vinelandii ; Azotobacter vinelandii - enzymology ; Azotobacter vinelandii - genetics ; Azotobacter vinelandii - ultrastructure ; Bacterial Proteins - genetics ; Bacterial Proteins - metabolism ; Bacterial Proteins - physiology ; Electrophoresis, Gel, Two-Dimensional ; Genes, Bacterial ; Methylphenazonium Methosulfate - pharmacology ; Mutation ; Oxidants - pharmacology ; Oxidative Stress - genetics ; Phenotype ; Polyhydroxybutyrate ; Protein profiles ; Proteomics ; rhdA mutant ; Rhodanese ; Thiosulfate Sulfurtransferase - deficiency ; Thiosulfate Sulfurtransferase - genetics ; Thiosulfate Sulfurtransferase - physiology</subject><ispartof>FEBS letters, 2007-04, Vol.581 (8), p.1625-1630</ispartof><rights>2007 Federation of European Biochemical Societies</rights><rights>FEBS Letters 581 (2007) 1873-3468 © 2015 Federation of European Biochemical Societies</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c5068-f27fa5103af9ce21cd2a1ce87b8c806cd363a60f6c21d9c4857feb6c851d80383</citedby><cites>FETCH-LOGICAL-c5068-f27fa5103af9ce21cd2a1ce87b8c806cd363a60f6c21d9c4857feb6c851d80383</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1016%2Fj.febslet.2007.03.028$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0014579307002888$$EHTML$$P50$$Gelsevier$$Hfree_for_read</linktohtml><link.rule.ids>314,776,780,1411,1427,3537,27901,27902,45550,45551,46384,46808,65306</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/17383639$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Cereda, Angelo</creatorcontrib><creatorcontrib>Carpen, Aristodemo</creatorcontrib><creatorcontrib>Picariello, Gianluca</creatorcontrib><creatorcontrib>Iriti, Marcello</creatorcontrib><creatorcontrib>Faoro, Franco</creatorcontrib><creatorcontrib>Ferranti, Pasquale</creatorcontrib><creatorcontrib>Pagani, Silvia</creatorcontrib><title>Effects of the deficiency of the rhodanese-like protein RhdA in Azotobacter vinelandii</title><title>FEBS letters</title><addtitle>FEBS Lett</addtitle><description>In
Azotobacter vinelandii the
rhdA gene codes for a protein (RhdA) of the rhodanese-homology superfamily. By combining proteomics, enzymic profiles and ultrastructural observations, the phenotype of an
A. vinelandii rhdA mutant was analyzed. We found that the
A. vinelandii rhdA mutant, and not the wild-type strain, accumulated polyhydroxybutyrate. RhdA deficiency enhanced the expression of enzymes of the polyhydroxybutyrate biosynthetic operon, and affected the activity of specific tricarboxylic acid cycle enzymes. The effect was dramatic on aconitase, in spite of comparable expression of aconitase polypeptides in both strains. By using a model system, we found that RhdA triggered protection from oxidants.</description><subject>Azotobacter vinelandii</subject><subject>Azotobacter vinelandii - enzymology</subject><subject>Azotobacter vinelandii - genetics</subject><subject>Azotobacter vinelandii - ultrastructure</subject><subject>Bacterial Proteins - genetics</subject><subject>Bacterial Proteins - metabolism</subject><subject>Bacterial Proteins - physiology</subject><subject>Electrophoresis, Gel, Two-Dimensional</subject><subject>Genes, Bacterial</subject><subject>Methylphenazonium Methosulfate - pharmacology</subject><subject>Mutation</subject><subject>Oxidants - pharmacology</subject><subject>Oxidative Stress - genetics</subject><subject>Phenotype</subject><subject>Polyhydroxybutyrate</subject><subject>Protein profiles</subject><subject>Proteomics</subject><subject>rhdA mutant</subject><subject>Rhodanese</subject><subject>Thiosulfate Sulfurtransferase - deficiency</subject><subject>Thiosulfate Sulfurtransferase - genetics</subject><subject>Thiosulfate Sulfurtransferase - physiology</subject><issn>0014-5793</issn><issn>1873-3468</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2007</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNUctOwzAQtBAIyuMTQDlxS1jHTeycUKnKQ0JC4nW1XHutuqQJ2CmofD0OLeIIp9WuZmdnZwg5ppBRoOXZPLM4DTV2WQ7AM2AZ5GKLDKjgLGXDUmyTAQAdpgWv2B7ZD2EOsRe02iV7lDPBSlYNyPPEWtRdSFqbdDNMDFqnHTZ69TPxs9aoBgOmtXvB5NW3HbomuZ-ZURLr6LPt2qnSHfrk3TVYq8Y4d0h2rKoDHm3qAXm6nDyOr9Pbu6ub8eg21QWUIrU5t6qgwJStNOZUm1xRjYJPhRZQahNFqhJsqXNqKj0UBY9fl1oU1AiIPxyQ0zVvlPW2xNDJhQsa66gC22WQOTAGBYcILNZA7dsQPFr56t1C-ZWkIHtD5VxuDJW9oRKYjIbGvZPNgeV0geZ3a-NgBFyvAR-uxtX_WOXl5CJ_6NPpwwEOcSb6W-drKoyOvTv0MnxHgcb5mJE0rftD7Rd7xJ9r</recordid><startdate>20070417</startdate><enddate>20070417</enddate><creator>Cereda, Angelo</creator><creator>Carpen, Aristodemo</creator><creator>Picariello, Gianluca</creator><creator>Iriti, Marcello</creator><creator>Faoro, Franco</creator><creator>Ferranti, Pasquale</creator><creator>Pagani, Silvia</creator><general>Elsevier B.V</general><scope>6I.</scope><scope>AAFTH</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>C1K</scope></search><sort><creationdate>20070417</creationdate><title>Effects of the deficiency of the rhodanese-like protein RhdA in Azotobacter vinelandii</title><author>Cereda, Angelo ; Carpen, Aristodemo ; Picariello, Gianluca ; Iriti, Marcello ; Faoro, Franco ; Ferranti, Pasquale ; Pagani, Silvia</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c5068-f27fa5103af9ce21cd2a1ce87b8c806cd363a60f6c21d9c4857feb6c851d80383</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2007</creationdate><topic>Azotobacter vinelandii</topic><topic>Azotobacter vinelandii - enzymology</topic><topic>Azotobacter vinelandii - genetics</topic><topic>Azotobacter vinelandii - ultrastructure</topic><topic>Bacterial Proteins - genetics</topic><topic>Bacterial Proteins - metabolism</topic><topic>Bacterial Proteins - physiology</topic><topic>Electrophoresis, Gel, Two-Dimensional</topic><topic>Genes, Bacterial</topic><topic>Methylphenazonium Methosulfate - pharmacology</topic><topic>Mutation</topic><topic>Oxidants - pharmacology</topic><topic>Oxidative Stress - genetics</topic><topic>Phenotype</topic><topic>Polyhydroxybutyrate</topic><topic>Protein profiles</topic><topic>Proteomics</topic><topic>rhdA mutant</topic><topic>Rhodanese</topic><topic>Thiosulfate Sulfurtransferase - deficiency</topic><topic>Thiosulfate Sulfurtransferase - genetics</topic><topic>Thiosulfate Sulfurtransferase - physiology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Cereda, Angelo</creatorcontrib><creatorcontrib>Carpen, Aristodemo</creatorcontrib><creatorcontrib>Picariello, Gianluca</creatorcontrib><creatorcontrib>Iriti, Marcello</creatorcontrib><creatorcontrib>Faoro, Franco</creatorcontrib><creatorcontrib>Ferranti, Pasquale</creatorcontrib><creatorcontrib>Pagani, Silvia</creatorcontrib><collection>ScienceDirect Open Access Titles</collection><collection>Elsevier:ScienceDirect:Open Access</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Environmental Sciences and Pollution Management</collection><jtitle>FEBS letters</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Cereda, Angelo</au><au>Carpen, Aristodemo</au><au>Picariello, Gianluca</au><au>Iriti, Marcello</au><au>Faoro, Franco</au><au>Ferranti, Pasquale</au><au>Pagani, Silvia</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Effects of the deficiency of the rhodanese-like protein RhdA in Azotobacter vinelandii</atitle><jtitle>FEBS letters</jtitle><addtitle>FEBS Lett</addtitle><date>2007-04-17</date><risdate>2007</risdate><volume>581</volume><issue>8</issue><spage>1625</spage><epage>1630</epage><pages>1625-1630</pages><issn>0014-5793</issn><eissn>1873-3468</eissn><abstract>In
Azotobacter vinelandii the
rhdA gene codes for a protein (RhdA) of the rhodanese-homology superfamily. By combining proteomics, enzymic profiles and ultrastructural observations, the phenotype of an
A. vinelandii rhdA mutant was analyzed. We found that the
A. vinelandii rhdA mutant, and not the wild-type strain, accumulated polyhydroxybutyrate. RhdA deficiency enhanced the expression of enzymes of the polyhydroxybutyrate biosynthetic operon, and affected the activity of specific tricarboxylic acid cycle enzymes. The effect was dramatic on aconitase, in spite of comparable expression of aconitase polypeptides in both strains. By using a model system, we found that RhdA triggered protection from oxidants.</abstract><cop>England</cop><pub>Elsevier B.V</pub><pmid>17383639</pmid><doi>10.1016/j.febslet.2007.03.028</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record> |
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| source | MEDLINE; Wiley Online Library Journals Frontfile Complete; Elsevier ScienceDirect Journals; Wiley Online Library Free Content; EZB-FREE-00999 freely available EZB journals; Alma/SFX Local Collection |
| subjects | Azotobacter vinelandii Azotobacter vinelandii - enzymology Azotobacter vinelandii - genetics Azotobacter vinelandii - ultrastructure Bacterial Proteins - genetics Bacterial Proteins - metabolism Bacterial Proteins - physiology Electrophoresis, Gel, Two-Dimensional Genes, Bacterial Methylphenazonium Methosulfate - pharmacology Mutation Oxidants - pharmacology Oxidative Stress - genetics Phenotype Polyhydroxybutyrate Protein profiles Proteomics rhdA mutant Rhodanese Thiosulfate Sulfurtransferase - deficiency Thiosulfate Sulfurtransferase - genetics Thiosulfate Sulfurtransferase - physiology |
| title | Effects of the deficiency of the rhodanese-like protein RhdA in Azotobacter vinelandii |
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